LOCUS       sequence-210077-         731 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses V5-APEX2-NES in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Benhalevy D, Anastasakis DG, Hafner M
  TITLE     Proximity-CLIP provides a snapshot of protein-occupied RNA elements 
            in subcellular compartments.
  JOURNAL   Nat Methods. 2018 Dec;15(12):1074-1082. doi: 
            10.1038/s41592-018-0220-y. Epub 2018 Nov 26.
  PUBMED    30478324
REFERENCE   2  (bases 1 to 731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-018-0220-y"; journalName: "Nat Methods"; date: 
            "2018-12"; volume: "15"; issue: "12"; pages: "1074-1082"
COMMENT     Sequence Label: pEXP(V5-APEX2-NES)
FEATURES             Location/Qualifiers
     source          1..731
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(189..293)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(368..387)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
       61 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
      121 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
      181 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
      241 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
      301 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca
      361 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg
      421 tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga
      481 ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac
      541 gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
      601 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
      661 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
      721 tagtaacgcc a
//
