LOCUS       sequence-210081-        8612 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Telomere-targeting Spy sgRNA under U6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8612)
  AUTHORS   Gao XD, Tu LC, Mir A, Rodriguez T, Ding Y, Leszyk J, Dekker J, 
            Shaffer SA, Zhu LJ, Wolfe SA, Sontheimer EJ
  TITLE     C-BERST: defining subnuclear proteomic landscapes at genomic 
            elements with dCas9-APEX2.
  JOURNAL   Nat Methods. 2018 Jun;15(6):433-436. doi: 10.1038/s41592-018-0006-2.
            Epub 2018 May 7.
  PUBMED    29735996
REFERENCE   2  (bases 1 to 8612)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-018-0006-2"; journalName: "Nat Methods"; date: 
            "2018-06"; volume: "15"; issue: "6"; pages: "433-436"
COMMENT     Sequence Label: pEJS613_pTetR-P2A-BFPnls/sgTelo
FEATURES             Location/Qualifiers
     source          1..8612
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     32..51
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     285..302
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    419..440
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(455..460,461..478,479..485)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    493..509
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     498..520
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     517..533
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     517..533
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     552..572
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        554..572
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        600..826
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             827..1007
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1054..1179
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1672..1905
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2090..2134
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2432..2680
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2432..2452
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2603..2622
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    2836..2953
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3002..3512
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3431..3449
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     intron          3521..4093
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     4101..4120
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        4148..4166
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4177..4797
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(4250..4269)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             4831..4887
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4903..5613
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGIYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             5614..5634
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     LTR             5730..5963
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6035..6156
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6072..6091)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6126..6145
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6196..6331
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(6352..6370)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6380..6397)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6380..6396)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6389..6411)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6538..6993
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6625..6644)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6835..6856
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7019..7123
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7124..7192,7193..7984)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7342..7361)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8155..8612,1..131)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
       61 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
      121 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
      181 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
      241 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
      301 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
      361 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
      421 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
      481 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
      541 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
      601 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
      661 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
      721 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
      781 gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt
      841 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
      901 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
      961 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     1021 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     1081 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     1141 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     1201 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     1261 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     1321 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     1381 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     1441 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     1501 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     1561 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     1621 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     1681 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     1741 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     1801 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     1861 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     1921 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     1981 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     2041 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     2101 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     2161 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     2221 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     2281 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
     2341 agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt atcgatcacg
     2401 agactagcct cgagcggccg cccccttcac cgagggccta tttcccatga ttccttcata
     2461 tttgcatata cgatacaagg ctgttagaga gataattgga attaatttga ctgtaaacac
     2521 aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt
     2581 tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga
     2641 tttcttggct ttatatatct tgtggaaagg acgaaacacc gttagggtta gggttagggt
     2701 tgtttaagag ctatgctgga aacagcatag caagtttaaa taaggctagt ccgttatcaa
     2761 cttgaaaaag tggcaccgag tcggtgcttt ttttgaattc tcgacctcga gacaaatggc
     2821 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
     2881 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
     2941 aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggc cgcggctcga
     3001 gggggttggg gttgcgcctt ttccaaggca gccctgggtt tgcgcaggga cgcggctgct
     3061 ctgggcgtgg ttccgggaaa cgcagcggcg ccgaccctgg gtctcgcaca ttcttcacgt
     3121 ccgttcgcag cgtcacccgg atcttcgccg ctacccttgt gggccccccg gcgacgcttc
     3181 ctgctccgcc cctaagtcgg gaaggttcct tgcggttcgc ggcgtgccgg acgtgacaaa
     3241 cggaagccgc acgtctcact agtaccctcg cagacggaca gcgccaggga gcaatggcag
     3301 cgcgccgacc gcgatgggct gtggccaata gcggctgctc agcagggcgc gccgagagca
     3361 gcggccggga aggggcggtg cgggaggcgg ggtgtggggc ggtagtgtgg gccctgttcc
     3421 tgcccgcgcg gtgttccgca ttctgcaagc ctccggagcg cacgtcggca gtcggctccc
     3481 tcgttgaccg aatcaccgac ctctctcccc agggggatcc gtgagtttgg ggacccttga
     3541 ttgttctttc tttttcgcta ttgtaaaatt catgttatat ggagggggca aagttttcag
     3601 ggtgttgttt agaatgggaa gatgtccctt gtatcaccat ggaccctcat gataattttg
     3661 tttctttcac tttctactct gttgacaacc attgtctcct cttattttct tttcattttc
     3721 tgtaactttt tcgttaaact ttagcttgca tttgtaacga atttttaaat tcacttttgt
     3781 ttatttgtca gattgtaagt actttctcta atcacttttt tttcaaggca atcagggtat
     3841 attatattgt acttcagcac agttttagag aacaattgtt ataattaaat gataaggtag
     3901 aatatttctg catataaatt ctggctggcg tggaaatatt cttattggta gaaacaacta
     3961 catcctggtc atcatcctgc ctttctcttt atggttacaa tgatatacac tgtttgagat
     4021 gaggataaaa tactctgagt ccaaaccggg cccctctgct aaccatgttc atgccttctt
     4081 ctttttccta cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa
     4141 agaattgtaa tacgactcac tatagggcga attgatatgt ctagattaga taaaagtaaa
     4201 gtgattaaca gcgcattaga gctgcttaat gaggtcggaa tcgaaggttt aacaacccgt
     4261 aaactcgccc agaagctagg tgtagagcag cctacattgt attggcatgt aaaaaataag
     4321 cgggctttgc tcgacgcctt agccattgag atgttagata ggcaccatac tcacttttgc
     4381 cctttagaag gggaaagctg gcaagatttt ttacgtaata acgctaaaag ttttagatgt
     4441 gctttactaa gtcatcgcga tggagcaaaa gtacatttag gtacacggcc tacagaaaaa
     4501 cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc aacaaggttt ttcactagag
     4561 aatgcattat atgcactcag cgctgtgggg cattttactt taggttgcgt attggaagat
     4621 caagagcatc aagtcgctaa agaagaaagg gaaacaccta ctactgatag tatgccgcca
     4681 ttattacgac aagctatcga attatttgat caccaaggtg cagagccagc cttcttattc
     4741 ggccttgaat tgatcatatg cggattagaa aaacaactta aatgtgaaag tgggtccgcg
     4801 tacagcggct cccgggagtt cgctagcggt gctactaact tcagcctgct gaagcaggct
     4861 ggagacgtgg aggagaaccc tggacctggt agtggaacgc gtatggtgtc taagggcgaa
     4921 gagctgatta aggagaacat gcacatgaag ctgtacatgg agggcaccgt ggacaaccat
     4981 cacttcaagt gcacatccga gggcgaaggc aagccctacg agggcaccca gaccatgaga
     5041 atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg acatcctggc tactagcttc
     5101 ctctacggca gcaagacctt catcaaccac acccagggca tccccgactt cttcaagcag
     5161 tccttccctg agggcttcac atgggagaga gtcaccacat acgaagatgg gggcgtgctg
     5221 accgctaccc aggacaccag cctccaggac ggctgcctca tctacaacgt caagatcaga
     5281 ggggtgaact tcacatccaa cggccctgtg atgcagaaga aaacactcgg ctgggaggcc
     5341 ttcaccgaga cgctgtaccc cgctgacggc ggcctggaag gcagaaacga catggccctg
     5401 aagctcgtgg gcgggagcca tctgatcgca aacgccaaga ccacatatag atccaagaaa
     5461 cccgctaaga acctcaagat gcctggcatc tactatgtgg actacagact ggaaagaatc
     5521 aaggaggcca acaacgagac ctacgtcgag cagcacgagg tggcagtggc cagatactgc
     5581 gacctcccta gcaaactggg gcacaagctt aatccaaaga aaaagcggaa agtgtgagtc
     5641 gacctcgagg gggggcccgg tacctttaag accaatgact tacaaggcag ctgtagatct
     5701 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaaa
     5761 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     5821 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     5881 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     5941 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
     6001 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
     6061 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     6121 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
     6181 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     6241 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     6301 cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg
     6361 agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     6421 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     6481 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg
     6541 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     6601 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     6661 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     6721 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     6781 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     6841 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     6901 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     6961 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg
     7021 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     7081 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     7141 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     7201 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     7261 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     7321 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     7381 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     7441 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     7501 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     7561 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     7621 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     7681 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     7741 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     7801 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     7861 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     7921 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     7981 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     8041 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     8101 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     8161 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     8221 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     8281 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     8341 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     8401 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     8461 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     8521 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     8581 ggcggacagg tatccggtaa gcggcagggt cg
//
