LOCUS       sequence-210082-        3817 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty AmpR pSC101 vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Segall-Shapiro TH, Sontag ED, Voigt CA
  TITLE     Engineered promoters enable constant gene expression at any copy 
            number in bacteria.
  JOURNAL   Nat Biotechnol. 2018 Mar 19. pii: nbt.4111. doi: 10.1038/nbt.4111.
  PUBMED    29553576
REFERENCE   2  (bases 1 to 3817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 Mar 19. pii: nbt.4111. doi: 10.1038/nbt.4111."
COMMENT     Sequence Label: pTHSSe_1
FEATURES             Location/Qualifiers
     source          1..3817
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(10..801,802..870))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     633..652
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      complement(1195..1238)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      1260..1317
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1341..1358)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1990..2940)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQITSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      2988..3210
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(3702..3796)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 gagtcccgct taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
       61 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
      121 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
      181 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
      241 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
      301 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
      361 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
      421 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
      481 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
      541 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
      601 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
      661 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
      721 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
      781 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
      841 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
      901 ttatcgcgaa acgatcctca tcctgtctct tgatcagatc ttgatcccct gcgccatcag
      961 atccttggcg gcaagaaagc catccagttt actttgcagg gcttcccaac cttaccagag
     1021 ggcgccccag ctggcaattc cgacgtctaa gaaaccatta ttatcatgac attaacctat
     1081 aaaaataggc gtatcacgag gccctttcgt cttcacctcg agtgccacct gacgtctaag
     1141 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca
     1201 gataaaaaaa atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgctgcagt
     1261 ccggcaaaaa agggcaaggt gtcaccaccc tgcccttttt ctttaaaacc gaaaagatta
     1321 cttcgcgtta tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     1381 cgagcggtat cagctcactc aaaggcggta atctgttgtt tgtcggtgaa cgctctcctg
     1441 agtaggacaa atccgccgcc ctagacctag ggtacgggtt ttgctgcccg caaacgggct
     1501 gttctggtgt tgctagtttg ttatcagaat cgcagatccg gcttcagccg gtttgccggc
     1561 tgaaagcact atttcttcca gaattgccat gattttttcc ccacgggagg cgtcactggc
     1621 tcccgtgttg tcggcagctt tgattcgata agcagcatcg cctgtttcag gctgtctatg
     1681 tgtgactgtt gagctgtaac aagttgtctc aggtgttcaa tttcatgttc tagttgcttt
     1741 gttttactgg tttcacctgt tctattaggt gttacatgct gttcatctgt tacattgtcg
     1801 atctgttcat ggtgaacagc tttgaatgca ccaaaaactc gtaaaagctc tgatgtatct
     1861 atctttttta caccgttttc atctgtgcat atggacagtt ttccctttga tatgtaacgg
     1921 tgaacagttg ttctactttt gtttgttagt cttgatgctt cactgataga tacaagagcc
     1981 ataagaacct cagatccttc cgtatttagc cagtatgttc tctagtgtgg ttcgttgttt
     2041 ttgcgtgagc catgagaacg aaccattgag atcatactta ctttgcatgt cactcaaaaa
     2101 ttttgcctca aaactggtga tctgaatttt tgcagttaaa gcatcgtgta gtgtttttct
     2161 tagtccgtta tgtaggtagg aatctgatgt aatggttgtt ggtattttgt caccattcat
     2221 ttttatctgg ttgttctcaa gttcggttac gagatccatt tgtctatcta gttcaacttg
     2281 gaaaatcaac gtatcagtcg ggcggcctcg cttatcaacc accaatttca tattgctgta
     2341 agtgtttaaa tctttactta ttggtttcaa aacccattgg ttaagccttt taaactcatg
     2401 gtagttattt tcaagcatta acatgaactt aaattcatca aggctaatct ctatatttgc
     2461 cttgtgagtt ttcttttgtg ttagttcttt taataaccac tcataaatcc tcatagagta
     2521 tttgttttca aaagacttaa catgttccag attatatttt atgaattttt ttaactggaa
     2581 aagataaggc aatatctctt cactaaaaac taattctaat ttttcgcttg agaacttggc
     2641 atagtttgtc cactggaaaa tctcaaagcc tttaaccaaa ggattcctga tttccacagt
     2701 tctcgtcatc agctctctgg ttgctttagc taatacacca taagcatttt ccctactgat
     2761 gttcatcatc tgagcgtatt ggttataagt gaacgatacc gtccgttctt tccttgtagg
     2821 gttttcaatc gtggggttga gtagtgccac acagcataaa attagcttgg tttcatgctc
     2881 cgttaagtca tagcgactaa tcgctagttc atttgctttg aaaacaacta attcagacat
     2941 acatctcaat tggtctaggt gattttaatc actataccaa ttgagatggg ctagtcaatg
     3001 ataattacta gtccttttcc tttgagttgt gggtatctgt aaattctgct agacctttgc
     3061 tggaaaactt gtaaattctg ctagaccctc tgtaaattcc gctagacctt tgtgtgtttt
     3121 ttttgtttat attcaagtgg ttataattta tagaataaag aaagaataaa aaaagataaa
     3181 aagaatagat cccagccctg tgtataactc actactttag tcagttccgc agtattacaa
     3241 aaggatgtcg caaacgctgt ttgctcctct acaaaacaga ccttaaaacc ctaaaggctt
     3301 aagtagcacc ctcgcaagct cgggcaaatc gctgaatatt ccttttgtct ccgaccatca
     3361 ggcacctgag tcgctgtctt tttcgtgaca ttcagttcgc tgcgctcacg gctctggcag
     3421 tgaatggggg taaatggcac tacaggcgcc ttttatggat tcatgcaagg aaactaccca
     3481 taatacaaga aaagcccgtc acgggcttct cagggcgttt tatggcgggt ctgctatgtg
     3541 gtgctatctg actttttgct gttcagcagt tcctgccctc tgattttcca gtctgaccac
     3601 ttcggattat cccgtgacag gtcattcaga ctggctaatg cacccagtaa ggcagcggta
     3661 tcatcaacag gcttacccgt cttactgtcc ctagtgcttg gattctcacc aataaaaaac
     3721 gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga
     3781 tctatcaaca ggagtccaag cgagctctcg aacccca
//
