LOCUS       sequence-21009-e         625 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 625)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG304GAL-ccdB-Cerulean
FEATURES             Location/Qualifiers
     source          1..625
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(84..208)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(285..306)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(546..565)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tgacatcaaa aacgccatta acctgatgtt ctggggaata taaatgtcag gctcccttat
       61 acacagccag tctgcaggtc gaccatagtg actggatatg ttgtgtttta cagtattatg
      121 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt
      181 ctcgttcagc tttcttgtac aaagtggtga tgggctgcag gaattcgata tcaagcttaa
      241 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
      301 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
      361 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
      421 tcgtgaccac cctgacctgg ggcgtgcagt gcttcgcccg ctaccccgac cacatgaagc
      481 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
      541 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
      601 tgaaccgcat cgagctgaag ggcat
//
