LOCUS       sequence-210111-        4356 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression vector for direct cardiac reprogramming.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4356)
  AUTHORS   Wang L, Liu Z, Yin C, Asfour H, Chen O, Li Y, Bursac N, Liu J, Qian 
            L
  TITLE     Stoichiometry of Gata4, Mef2c, and Tbx5 influences the efficiency 
            and quality of induced cardiac myocyte reprogramming.
  JOURNAL   Circ Res. 2015 Jan 16;116(2):237-44. doi: 
            10.1161/CIRCRESAHA.116.305547. Epub 2014 Nov 21.
  PUBMED    25416133
REFERENCE   2  (bases 1 to 4356)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1161/CIRCRESAHA.116.305547"; journalName: "Circ Res"; date: 
            "2015-01-16- 16"; volume: "116"; issue: "2"; pages: "237-44"
COMMENT     Sequence Label: pMx-MGT
FEATURES             Location/Qualifiers
     source          1..4356
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1333..1389
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2734..2787
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 ggatcctcta gagaattcgc catggggaga aaaaagattc agattacgag gataatggat
       61 gagcgtaaca gacaggtgac ttttacgaag aggaaatttg gattgatgaa gaaggcttat
      121 gagctgagcg tgctgtgcga ctgtgagatt gcactgatca tcttcaacag caccaacaag
      181 ctgttccagt acgccagcac tgacatggat aaggtgttgc tcaagtacac cgagtacaac
      241 gagccgcacg agagccggac aaactcagac attgtggagg cattgaacaa gaaagaaaac
      301 aaaggctctg aaagccccga tcctgactcc tcttatgcac tcaccccacg cactgaagaa
      361 aaatacaaaa aaattaatga agaatttgat aatatgatca agagtcataa aattcctgct
      421 gttccacctc ccagctttga gatgccagtt accatcccag tgtccagcca taacagtttg
      481 gtgtacagca atcctgtcag cacactggga aaccccaatc ttctgccact ggcccacccg
      541 tctctgcaga ggaatagtat gtctcctggt gtaacacata gacctccaag tgcaggtaac
      601 acaggcggtc tgatgggcgg agatctgaca tccggtgcag gcaccagcgc agggaatgga
      661 tacggcaacc cccggaactc accaggcctg ctggtctcac ctggtaacct gaacaagaat
      721 atacaagcca aatctcctcc ccctatgaat ctaggaatga ataatcgtaa gccagatctc
      781 cgcgttctta tcccacctgg cagcaagaac acgatgccat cagtgaatca aaggataaat
      841 aactcccagt cggctcagtc attggctacc ccggtggttt ccgtagcaac tcctacttta
      901 ccaggacaag gaatgggagg atatccatca gccatttcaa caacatatgg tactgagtac
      961 tctctgagta gcgcagatct gtcatctctg tctggcttca acactgccag tgcgctccac
     1021 ctcggctctg taactggctg gcagcagcag cacctacata acatgccgcc atctgccctc
     1081 agtcagttgg gagaccgtac caccacccct tcgagatacc cacaacacac cacgcgccac
     1141 gaggcgggga ggtctcctgt tgacagcttg agcagctgta gcagttccta cgatgggagc
     1201 gaccgagagg atcaccggaa cgaattccac tcccccattg gactcaccag accttcgccg
     1261 gacgaaaggg aaagtccttc agtcaagcgc atgcgactct ctgaaggatg ggcaacagct
     1321 agcggcagcg gcgccacaaa cttctctctg ctaaagcaag caggtgatgt tgaagaaaac
     1381 cccgggcctg catgcatgta ccaaagcctg gccatggccg ccaaccacgg ccccccgccc
     1441 ggcgcctacg aagcaggtgg ccctggcgcc ttcatgcaca gcgcgggcgc cgcgtcctcg
     1501 cccgtctacg tgcccactcc gcgggtgccg tcctctgtgc tgggcctgtc ctacctgcag
     1561 ggcggtggca gtgccgctgc agctggaacc acctcgggtg gcagctccgg ggccggcccg
     1621 tcgggtgcag ggcctgggac ccagcagggt agccctggct ggagccaagc tggagccgag
     1681 ggagccgcct acaccccgcc gcccgtgtcc ccgcgcttct ctttcccggg gactactggg
     1741 tccctggcgg ccgctgccgc cgctgccgca gcccgggaag ctgcagccta cggcagtggc
     1801 ggcggggcgg cgggcgctgg tctggctggc cgagagcagt acgggcgtcc gggcttcgcc
     1861 ggctcctact ccagccccta cccagcctac atggccgacg tgggagcatc ctgggccgca
     1921 gccgctgccg cctctgccgg ccccttcgac agcccagtcc tgcacagcct gcctggacgg
     1981 gccaaccctg gaagacaccc caatctcgat atgtttgatg acttctcaga aggcagagag
     2041 tgtgtcaatt gtggggccat gtccacccca ctctggaggc gagatgggac gggacactac
     2101 ctgtgcaatg cctgtggcct ctatcacaag atgaacggca tcaaccggcc cctcattaag
     2161 cctcagcgcc gcctgtccgc ttcccgccgg gtaggcctct cctgtgccaa ctgccagact
     2221 accaccacca cgctgtggcg tcgtaatgcc gagggtgagc ctgtatgtaa tgcctgcggc
     2281 ctctacatga agctccatgg ggttcccagg cctcttgcaa tgcggaagga ggggattcaa
     2341 accagaaaac ggaagcccaa gaacctgaat aaatctaaga cgccagcagg tcctgctggt
     2401 gagaccctcc ctccctccag tggtgcctcc agcggtaact ccagcaatgc cactagcagc
     2461 agcagcagca gtgaagagat gcgccccatc aagacagagc ccgggctgtc atctcactat
     2521 gggcacagca gctccatgtc ccagacattc agtactgtgt ccggccacgg gccctccatc
     2581 catccagtgc tgtctgctct gaagctgtcc ccacaaggct atgcatctcc tgtcactcag
     2641 acatcgcagg ccagctccaa gcaggactct tggaacagcc tggtcctggc tgacagtcat
     2701 ggggacataa tcaccgcggc atgcggctcc ggcgagggca ggggaagtct tctaacatgc
     2761 ggggacgtgg aggaaaatcc cggcccactc gagatggccg atacagatga gggctttggc
     2821 ctggcgcgca cgcctctgga gcctgattcc aaagacaggt cttgcgattc gaaacctgag
     2881 agtgctctgg gggctcccag caagtctcca tcatccccgc aggctgcctt cacccagcag
     2941 ggcatggaag gaatcaaggt gtttcttcat gaacgtgaac tgtggctgaa gttccacgaa
     3001 gtgggcacag agatgatcat caccaaggca gggaggagaa tgtttcctag ttacaaagtg
     3061 aaggtgactg gccttaatcc caaaacgaag tatattcttc tcatggatat tgttcccgca
     3121 gacgaccaca gatataaatt tgctgataac aaatggtccg taactggcaa agcagagcct
     3181 gccatgccgg ggcgccttta cgtgcacccg gactccccag caaccggagc ccactggatg
     3241 cgacaacttg tctccttcca gaagctcaaa ctcaccaaca accacctgga cccgtttgga
     3301 cacattatcc tgaactccat gcacaaatac cagccccgat tacacatcgt gaaagcagac
     3361 gaaaataatg ggttcggttc aaagaacact gcgttttgca cccacgtctt cccggagaca
     3421 gcttttatcg ctgtgacttc gtaccagaat cacaagatca cacagctgaa aattgagaac
     3481 aaccccttcg ccaaaggctt tcggggcagt gatgacctgg agttacacag gatgtctcgg
     3541 atgcaaagta aagagtatcc tgtggttccc aggagcacag tgaggcacaa agtcacctcc
     3601 aaccacagcc ccttcagcag cgagacccga gctctctcca cctcatccaa tttagggtcc
     3661 cagtaccagt gtgagaatgg tgtctctggc ccctcccagg accttctgcc cccacctaac
     3721 ccatacccac tggcccagga gcacagccaa atttaccact gtaccaagag gaaagatgag
     3781 gaatgttcca gcacggagca cccctataag aagccgtaca tggagacatc ccccagcgag
     3841 gaagacacct tctatcgctc gggctacccc cagcagcagg gcctgagtac ctcttacagg
     3901 acagagtcgg cccagcggca ggcctgcatg tatgccagct ccgctccccc cagcgagccc
     3961 gtgcctagcc tggaggacat cagctgtaac acatggccca gcatgccctc ctatagcagc
     4021 tgtaccgtca ccaccgtgca gcccatggac cgtcttccct accagcactt ctccgctcat
     4081 ttcacctcgg ggcccctggt ccctcggttg gctggcatgg ccaaccatgg ttctccccag
     4141 ctcggcgaag ggatgtttca gcaccagacc tcagtggccc atcagcctgt ggtcaggcag
     4201 tgcgggcctc agactggcct tcagtctccg ggcggcctcc agcccccaga gtttctctac
     4261 actcacggcg tgcccaggac cctgtccccc catcagtatc actcggtaca cggcgtcggc
     4321 atggtgccag agtggagtga gaatagctaa gtcgac
//
