LOCUS       sequence-210123-        8770 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Anion channelrhodopsin GtACR1 fused to ER export signal and targeted
            to the neuronal soma and proximal dendrites under the control of 
            internal ribosome entry sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8770)
  AUTHORS   Mardinly AR, Oldenburg IA, Pegard NC, Sridharan S, Lyall EH, Chesnov
            K, Brohawn SG, Waller L, Adesnik H
  TITLE     Precise multimodal optical control of neural ensemble activity.
  JOURNAL   Nat Neurosci. 2018 Apr 30. pii: 10.1038/s41593-018-0139-8. doi: 
            10.1038/s41593-018-0139-8.
  PUBMED    29713079
REFERENCE   2  (bases 1 to 8770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2018 Apr 30. pii: 10.1038/s41593-018-0139-8. doi: 
            10.1038/s41593-018-0139-8."
COMMENT     Sequence Label: pCAG-H2B-mRuby3-IRES-eGtACR1-ST
FEATURES             Location/Qualifiers
     source          1..8770
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..659
                     /label=chicken beta-actin promoter
     intron          660..1676
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1600..1622
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1684..1703
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1765..2142
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             2152..2862
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby2, with improved brightness and photostability (Bajar 
                     et al., 2016)"
                     /label=mRuby3
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTD
                     IALKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAV
                     AKYSNLGGGMDELYK"
     misc_feature    2897..3470
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2920..3472
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3064..3081)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3291..3310
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4398..4421
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     intron          4662..4794
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     4777..4796
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    4803..5391
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4856..4876)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5274..5285)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5418..5435)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5424..5535
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5631..5651)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5631..5649)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5670..5686)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5788..5807)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5853..5908
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5907..5926)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6269..6285)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6293..6309
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6317..6323,6324..6341,6342..6347))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6362..6383
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6442..6637
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6488..6623
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6643..6777
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6680..6699)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6734..6753
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6845..6862)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7016..7604)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7096..7115)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7775..8566,8567..8635))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8398..8417
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8636..8740)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggggggggg ggcgcgcgcc nggggggggc ggggcggggc gaggggcggg
      541 gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc
      601 cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg
      661 gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc
      721 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc
      781 cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa
      841 gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct
      961 gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg
     1021 gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
     1081 gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc
     1141 cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg
     1201 gcgcggggct cgccgtgccg ggcggggggk ggcggcaggt gggggtgccg ggcggggcgg
     1261 ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg agcgccggcg
     1321 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc
     1381 agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc
     1441 cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg
     1501 gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc
     1561 ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga
     1621 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc
     1681 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattc ggtaccgcgg
     1741 gcccgggatc caccggtagc ggccatgcca gagccagcga agtctgctcc cgccccgaaa
     1801 aagggctcca agaaggcggt gactaaggcg cagaagaaag gcggcaagaa gcgcaagcgc
     1861 agccgcaagg agagctattc catctatgtg tacaaggttc tgaagcaggt ccaccctgac
     1921 accggcattt cgtccaaggc catgggcatc atgaattcgt ttgtgaacga cattttcgag
     1981 cgcatcgcag gtgaggcttc ccgcctggcg cattacaaca agcgctcgac catcacctcc
     2041 agggagatcc agacggccgt gcgcctgctg ctgcctgggg agttggccaa gcacgccgtg
     2101 tccgagggta ctaaggccat caccaagtac accagcgcta aggcggccgc tatggtgtct
     2161 aagggcgaag agctgatcaa ggaaaatatg cgtatgaagg tggtcatgga aggttcggtc
     2221 aacggccacc aattcaaatg cacaggtgaa ggagaaggca gaccgtacga gggagtgcaa
     2281 accatgagga tcaaagtcat cgagggagga cccctgccat ttgcctttga cattcttgcc
     2341 acgtcgttca tgtatggcag ccgtaccttt atcaagtacc cggccgacat ccctgatttc
     2401 tttaaacagt cctttcctga gggttttact tgggaaagag ttacgagata cgaagatggt
     2461 ggagtcgtca ccgtcacgca ggacaccagc cttgaggatg gcgagctcgt ctacaacgtc
     2521 aaggtcagag gggtaaactt tccctccaat ggtcccgtga tgcagaagaa gaccaagggt
     2581 tgggagccta atacagagat gatgtatcca gcagatggtg gtctgagagg atacactgac
     2641 atcgcactga aagttgatgg tggtggccat ctgcactgca acttcgtgac aacttacagg
     2701 tcaaaaaaga ccgtcgggaa catcaagatg cccggtgtcc atgccgttga tcaccgcctg
     2761 gaaaggatcg aggagagtga caatgaaacc tacgtagtgc aaagagaagt ggcagttgcc
     2821 aaatacagca accttggtgg tggcatggac gagctgtaca agtgaattcc tcgactagaa
     2881 ttgggcccgg gatccgcccc tctccctccc ccccccctaa cgttactggc cgaagccgct
     2941 tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg
     3001 gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt
     3061 cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg
     3121 aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac
     3181 ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg
     3241 cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct
     3301 caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg
     3361 atctggggcc tcggtacaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag
     3421 gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa tatggccaca
     3481 gcttgtccac catgctcgag tccatgagca gcatcacctg tgatcccgcc atctacggcg
     3541 aatggagcag ggagaaccag ttctgcgtgg agaagagcct gatcaccctg gacggcatca
     3601 agtacgtcca gctggtgatg gccgtcgtga gcgcctgtca ggtgttcttc atggtgacca
     3661 gagcccccaa ggtgccctgg gaagccatct acctgcccac caccgagatg atcacctatt
     3721 ccctggcctt caccggaaac ggctacatca gagtggccaa tggcaagtac ctgccctggg
     3781 ccagaatggc cagctggctg tgcacctgcc ctatcatgct gggcctggtg agcaatatgg
     3841 ccctcgtgaa gtacaagagc atccccctca accctatgat gatcgccgcc tccagcatct
     3901 gcaccgtgtt cggcatcacc gcctccgtgg tgctagaccc gctgcacgtg tggctgtact
     3961 gtttcatcag cagcatcttc ttcatcttcg agatggtggt ggccttcgcc attttcgcca
     4021 ttaccatcca cgatttccag accatcggct cccccatgtc cctgaaggtg gtggagaggc
     4081 tgaagctgat gaggatcgtg ttctacgtga gctggatggc ctaccctatc ctgtggagct
     4141 tctccagcac cggcgcctgc atcatgagcg agaacaccag cagcgtgctg tacctgctgg
     4201 gcgacgctct gtgcaagaac acctacggca tcctgctgtg ggctacaacc tggggcctgc
     4261 tgaacggcaa gtgggacagg gattacgtga agggcaggaa cgtggacggc accctgatgc
     4321 ctgagtacga gcaggacctg gagaagggca acaccgagag gtacgaggac gccagagccg
     4381 gcgagaccgc accggtagat tacaaagacg atgacgataa gcagtcccag cccatcctca
     4441 acaccaagga gatggccccg cagagcaagc ctccagagga gctggagatg agcagcatgc
     4501 ccagccccgt ggcccctctg cccgcacgca cggagggcgt catcgacatg cggagcatgt
     4561 ccagcattga cagcttcatc agctgtgcca cggacttccc tgaagccacc agattcttct
     4621 gctacgagaa cgaggtgtga ggcgcgccct cgaggccgca ggtaagtatc aaggttacaa
     4681 gacaggttta aggagaccaa tagaaactgg gcttgtcgag acagagaaga ctcttgcgtt
     4741 tctgataggc acctattggt cttactgaca tccactttgc ctttctctcc acaggtgtcg
     4801 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     4861 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     4921 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     4981 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     5041 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     5101 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     5161 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc
     5221 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     5281 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     5341 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaattc
     5401 gagctcggta cgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     5461 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     5521 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     5581 gcaggacagc aagggggagg attgggaaga caatagccca gcttttgttc cctttagtga
     5641 gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     5701 ccgctaattc actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa
     5761 tgccctggct cacaaatacc actgagatct ttttccctct gccaaaaatt atggggacat
     5821 catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat
     5881 agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt
     5941 aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgcccata tgctggctgc
     6001 catgaacaaa ggttggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca
     6061 ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt
     6121 gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt
     6181 cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgga gatccctcga
     6241 cctgcagccc aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     6301 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     6361 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     6421 acctgtcgtg ccagcggatc cgcatctcaa ttagtcagca accatagtcc cgcccctaac
     6481 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     6541 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     6601 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctaacttgtt tattgcagct
     6661 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     6721 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatccgc
     6781 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     6841 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     6901 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     6961 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     7021 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     7081 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     7141 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     7201 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     7261 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     7321 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     7381 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     7441 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     7501 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     7561 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     7621 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     7681 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     7741 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     7801 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     7861 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     7921 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     7981 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     8041 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     8101 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     8161 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     8221 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     8281 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     8341 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     8401 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     8461 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     8521 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     8581 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     8641 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     8701 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     8761 cacctgggtc
//
