LOCUS       sequence-210128-        6640 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry vector for adenoviral expression of Ecdysone receptor-based 
            inducible transgene expression switch..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6640)
  AUTHORS   Lee S, Sohn KC, Choi DK, Won M, Park KA, Ju SK, Kang K, Bae YK, Hur 
            GM, Ro H
  TITLE     Ecdysone Receptor-based Singular Gene Switches for Regulated 
            Transgene Expression in Cells and Adult Rodent Tissues.
  JOURNAL   Mol Ther Nucleic Acids. 2016 Sep 27;5(9):e367. doi: 
            10.1038/mtna.2016.74.
  PUBMED    27673563
REFERENCE   2  (bases 1 to 6640)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Nucleic Acids."; date: "2016-09-27"; volume: "5(9):e367. doi"; 
            pages: " 10.1038/mtna.2016.74"
COMMENT     Sequence Label: pENTR-EUI
FEATURES             Location/Qualifiers
     source          1..6640
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     enhancer        837..1216
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1217..1420
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1371..1391
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        1465..1483
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1503..1940
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLQSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTV"
     promoter        complement(3045..3063)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3083..3100)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3089..3313
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    4039..4059
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        4061..4079
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     4104..4120
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     4105..4121
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4460..4478
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    4592..4726
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4616..4635)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4670..4689
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    complement(4765..4864)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(4882..4900)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4905..4921)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4905..4921)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5023..5042)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             5034..5843
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(5101..5120)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      5990..6578
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6479..6498
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
      721 ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
      781 ccgcatagtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      841 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      901 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      961 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     1021 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     1081 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     1141 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     1201 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
     1261 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
     1321 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
     1381 cggtaggcgt gtacggtggg aggtctatat aagcagagct ctctggctaa ctagagaacc
     1441 cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc aagcttatcg
     1501 atatgaagct acagtcttct atcgaacaag catgcgatat ttgccgactt aaaaagctca
     1561 agtgctccaa agaaaaaccg aagtgcgcca agtgtctgaa gaacaactgg gagtgtcgct
     1621 actctcccaa aaccaaaagg tctccgctga ctagggcaca tctgacagaa gtggaatcaa
     1681 ggctagaaag actggaacag ctatttctac tgatttttcc tcgagaagac cttgacatga
     1741 ttttgaaaat ggattcttta caggatataa aagcattgtt aacaggatta tttgtacaag
     1801 ataatgtgaa taaagatgcc gtcacagata gattggcttc agtggagact gatatgcctc
     1861 taacattgag acagcataga ataagtgcga catcatcatc ggaagagagt agtaacaaag
     1921 gtcaaagaca gttgactgta gagttcctgg atatggccga cttcgagttt gagcagatgt
     1981 ttaccgatgc ccttggaatt gacgagtacg gtgggctgca gatgaggcct gaatgtgtca
     2041 tacaggagcc cagtaaaaat aaagacaggc aaagacaaaa gaaagacaaa ggaatattat
     2101 tacctgttag tacgaccaca gtcgaagacc acatgccccc gatcatgcaa tgtgatccac
     2161 ctccgcccga ggccgccagg attcacgaag tcgtcccgag gtatctttcg gagaagctga
     2221 tggagcagaa caggcagaag aacataccac cattgtcggc gaatcagaag tctctgatcg
     2281 cgaggctcgt gtggtaccag gagggatatg agcagccctc cgacgaggat ctcaaaagag
     2341 taacgcagac ttggcagtcg gatgaagagg acgaggaatc cgatctaccc ttccgccaga
     2401 tcacggagat gacgatctta acggtccagt tgatcgtcga gttcgccaag ggtctaccgg
     2461 gcttttcgaa gatatcacag tctgatcaaa tcaccttatt aaaagcctcg tccagcgagg
     2521 tgatgatgct gcgggtggcg aggcgatacg acgccgcgtc cgacagcatt ctgttcgcca
     2581 acaacaaggc gtacacgcgc gacaactacc gcaaggcggg catggccgag gtcatcgaag
     2641 acctcctaca cttctgccgg tgcatgttcg cgatgggcat ggacaatgtg cactttgcac
     2701 tgctcacggc catcgttata ttctcagatc ggcccgggct cgagcagccg tcgctggtag
     2761 aagagatcca gagatactac ctgaacacgt tgcgaattta catcatcaac cagaacagcg
     2821 cgtcgtcgcg ctgcgccgtg atctacggca ggatcctgag cgtgctgacc gagctacgca
     2881 cgctcggcac gcaaaactcc aacatgtgca tctcgctgaa gctgaagaac aggaagctgc
     2941 cgccgttcct cgaggagatc tgggacgtgg cggaggtggc cacgacgcat cccacggtgc
     3001 tgccgcccac caacccggtg gtgctatagt ctagagggcc ctattctata gtgtcaccta
     3061 aatgctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     3121 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     3181 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     3241 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc cggggatgcg
     3301 gtgggctcta tggcttctga ggcggaaagt tgagaggaca ttccaatcat aggctgccca
     3361 tccaccctct gtgtcctcct gttaattagg tcacttaaca aaaaggaaat tgggtagggg
     3421 tttttcacag accgctttct aagggtaatt ttaaaatatc tgggaagtcc cttccactgc
     3481 tgtgttccag aagtgttggt aaacagccca caaatgtcaa cagcagaaac atacaagctg
     3541 tcagctttgc acaagggccc aacaccctgc tcatcaagaa gcactgtggt tgctgtgtta
     3601 gtaatgtgca aaacaggagg cacattttcc ccacctgtgt aggttccaaa atatctagtg
     3661 ttttcatttt tacttggatc aggaacccag cactccactg gataagcatt atccttatcc
     3721 aaaacagcct tgtggtcagt gttcatctgc tgactgtcaa ctgtagcatt ttttggggtt
     3781 acagtttgag caggatattt ggtcctgtag tttgctaaca caccctgcag ctccaaaggt
     3841 tccccaccaa cagcaaaaaa atgaaaattt gacccttgaa tgggttttcc agcaccattt
     3901 tcatgagttt tttgtgtccc tgaatgcaag tttaacatag cagttacccc aataacctca
     3961 gttttaacag taacagcttc ccacatcaaa atatttccac aggttaagtc ctcatttaaa
     4021 ttaggcaaag gaattgctca actttgtata gaaaagttgg taatacgact cactataggg
     4081 cgaattgggt accgggcccc ccctcgaggt cgacggtatc gataagctta ggcctccaag
     4141 gcggagtact gtcctccggg ctggcggagt actgtcctcc ggcaaggtcg gagtactgtc
     4201 ctccgacact agaggtcgga gtactgtcct ccgacgcaag gcggagtact gtcctccggg
     4261 ctgcggagta ctgtcctccg gcaaggtcgg agtactgtcc tccgacacta gaggtcggag
     4321 tactgtcctc cgacgcaagg cggagtactg tcctccgggc tggcggagta ctgtcctccg
     4381 gcaagggtcg actctagagg gtatataatg gatcccatcg cgtctcagcc tcactttgag
     4441 ctcctccaca cggagcttga tttaggtgac actatagaat acaagctact tgttcttttt
     4501 gcaggatccc atcgattcga attcacgtgc tagcgcttaa gaaattcaag gcctctcgag
     4561 cctctagaac tatagtgagt cgtattacgt agatccagac atgataagat acattgatga
     4621 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     4681 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     4741 cattcatttt atgtttcagg gccgacccag ctttcttgta caaagttggc attataagaa
     4801 agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta
     4861 tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct
     4921 ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat
     4981 catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc
     5041 atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct gatttatatg
     5101 ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg
     5161 ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg
     5221 ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca
     5281 agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa
     5341 cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg
     5401 cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc
     5461 gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg
     5521 attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac
     5581 ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta
     5641 tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc
     5701 gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga
     5761 aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt
     5821 tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac tggcagagca
     5881 ttacgctgac ttgacgggac ggcgcaagct catgaccaaa atcccttaac gtgagttacg
     5941 cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     6001 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     6061 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     6121 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     6181 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     6241 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     6301 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     6361 tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     6421 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     6481 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     6541 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     6601 tacggttcct ggccttttgc tggccttttg ctcacatgtt
//
