LOCUS       sequence-210145-       11602 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vinculin tension sensor with point mutation affecting ability to 
            bind actin in lentiviral expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11602)
  AUTHORS   Rothenberg KE, Scott DW, Christoforou N, Hoffman BD
  TITLE     Vinculin Force-Sensitive Dynamics at Focal Adhesions Enable 
            Effective Directed Cell Migration.
  JOURNAL   Biophys J. 2018 Apr 10;114(7):1680-1694. doi: 
            10.1016/j.bpj.2018.02.019.
  PUBMED    29642037
REFERENCE   2  (bases 1 to 11602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bpj.2018.02"; journalName: "Biophys J"; date: 
            "2018-04-10- 10"; volume: "114"; issue: "7"; pages: "1680-1694"
COMMENT     Sequence Label: pRRL-VinculinTS-I997A
FEATURES             Location/Qualifiers
     source          1..11602
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    12..128
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        188..567
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        568..771
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     722..742
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        816..834
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3528..4235
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     CDS             join(4356..4358,4359..4361,4362..5072)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4401..4422)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4662..4681)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5009..5030
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5646..6234
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5699..5719)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6117..6128)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6325..6558
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6630..6751
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6667..6686)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6721..6740
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6791..6926
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(6947..6965)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6975..6992)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6975..6991)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6984..7006)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7136..7591
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7223..7242)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7433..7454
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7620..7724
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7725..7793,7794..8585)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7943..7962)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8756..9344
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9245..9264
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9498..9515
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9632..9653
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9668..9673,9674..9691,9692..9698)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9706..9722
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9711..9733
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9730..9746
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9730..9746
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9765..9785
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9767..9785
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9813..10039
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             10040..10220
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10267..10392
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10885..11118
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11303..11347
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tatcggttaa cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
       61 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaattc
      121 aaaattttat cgatggatcc cccgggctcg agttcgaaga tcgatgtacg ggccagatat
      181 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
      241 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      301 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      361 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
      421 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      481 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      541 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
      601 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
      661 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
      721 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta
      781 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
      841 caagctggct agcgtttaaa cttaagcttg ccatgcccgt cttccacacg cgcaccatcg
      901 agagcatctt ggagcccgtg gctcagcaga tctcccacct ggtcatcatg cacgaggagg
      961 gggaggtaga cggcaaggcc atcccggacc tcaccgcccc cgtgtcggcc gtgcaggccg
     1021 ctgtcagcaa cctggtgcgg gttggaaaag aaactgtgca gacaacagaa gaccagatct
     1081 tgaaaaggga tatgccacca gcattcatca aagtagagaa tgcctgcacc aagctcgttc
     1141 gagcagccca gatgctgcaa gcagatcctt attcagtacc agctcgtgac tacctaattg
     1201 atggatcaag aggcatcctt tctggaacat cagacttact tctgacattt gatgaagcgg
     1261 aggtccgtaa aatcatccgt gtctgcaaag gaatattgga atatctgact gtggcagaag
     1321 tagtagagac tatggaggat ttggtgacat atacaaagaa tctagggcca ggaatgacaa
     1381 agatggcgaa aatgatcgat gagagacaac aggaattaac tcatcaggaa catagggtta
     1441 tgctggtgaa ctccatgaat actgtgaagg agctattgcc tgtacttatt tcagctatga
     1501 agatctttgt aaccacaaaa aacactaaaa gccagggaat agaagaggcc ttgaaaaatc
     1561 gcaatttcac agtagagaaa atgagtgctg agataaatga aataatccgt gtattacaac
     1621 tcacttcctg ggatgaagat gcctgggcca gcaaggacac tgaagccatg aaaagagctt
     1681 tagccctaat agattcaaag atgaaccagg caaaaggctg gctgagagat ccaaacgcac
     1741 ctccagggga tgctggtgag caagcaataa ggcagatcct tgatgaagct ggaaaagcag
     1801 gagaattgtg tgcaggcaaa gaacgcaggg agattctggg aacatgcaaa actttgggcc
     1861 aaatgactga tcaacttgct gatctccgag ctagaggaca gggtgctaca ccgatggcaa
     1921 tgcagaaggc acagcaggtg tcacaaggcc tggatttgct cactgcaaaa gtggagaatg
     1981 cagcccggaa attggaggcc atgacaaact ctaagcaggc tattgcaaag aagattgatg
     2041 ctgctcagaa ctggcttgcg gatcccaacg gcggaagtga aggagaagaa cacattcgag
     2101 gaattatgtc tgaagcaagg aaagttgcag aattgtgtga ggagcctaaa gaaagagatg
     2161 atatccttcg ctccttgggg gaaatctctg ctctgacagc taagctgtca gatctgcgac
     2221 gacatgggaa aggcgactct cctgaggccc gtgcattggc caagcaaata gctacatcac
     2281 ttcagaactt acagtccaaa acaaacagag ctgtagcaaa tactaggcca gttaaagctg
     2341 ctgtccattt ggagggcaag attgagcaag cccagaggtg gatagataat cctacagttg
     2401 atgatcgggg agtaggccag gcagcaattc ggggtttggt tgcagaaggt cgtcgtctag
     2461 ccaatgtcat gatgggacct tatcgtcagg acctgcttgc caaatgtgac cgtgtagacc
     2521 agctggctgc tcagcttgct gaccttgcag caagagggga aggagagtct cctcaggcta
     2581 gggcaattgc tgctcagctt caggactccc tgaaggatct caaagcacgg atgcaagaag
     2641 caatgaccca ggaggtgtct gatgttttca gtgacactac aacccctatt aagttgttag
     2701 cagtagcagc cactgctcct tctgatactc ccaatagaga agaggtgttt gaagaaagag
     2761 cagcaaattt tgaaaaccat gctgctagac tgggagcaac agcagaaaaa gcagctgcag
     2821 ttggaactgc taataaaact actgtggaag gcattcaggc aacagtcaaa tctgcaaggg
     2881 agcttacacc acaggtagta tcggctgctc gaatcctcct gagaaatcct ggaaatcaag
     2941 ctgcttatga gcattttgag acaatgaaaa accaatggat tgataatgta gaaaagatga
     3001 cagggctggt ggatgaggcc atcgatacca agtctctgtt ggatgcatca gaagaggcta
     3061 ttaagaagga tcttgataaa tgtaaagttg caatggccaa tatgcaacct cagatgctgg
     3121 tagctggagc caccagcatt gctagacgag cgaaccgcat cctgcttgtg gcaaaacggg
     3181 aggttgaaaa ttcagaagac cctaaattca gggaggctgt taaagcagct tctgatgagc
     3241 tgagcaaaac catatcaccg atggtaatgg atgctaaagc tgtagcagga aatatctctg
     3301 atcctggttt gcagaagagt ttcttggatt ctggatacag gattctggga gctgtggcca
     3361 aagtcagaga agcctttcag cctcaggagc cagactttcc ccctcctcct cctgacctag
     3421 agcatctcca tctgactgat gagcttgctc ctccaaaacc accacttcca gaaggtgagg
     3481 ttcccccacc cagaccacca ccccctgaag aaaaagatga ggtcgagatg gtgagcaagg
     3541 gcgaggagac cacaatgggc gtaatcaagc ccgacatgaa gatcaagctg aagatggagg
     3601 gcaacgtgaa tggccacgcc ttcgtgatcg agggcgaggg cgagggcaag ccctacgacg
     3661 gcaccaacac catcaacctg gaggtgaagg agggagcccc cctgcccttc tcctacgaca
     3721 ttctgaccac cgcgttcgcc tacggcaaca gggccttcac caagtacccc gacgacatcc
     3781 ccaactactt caagcagtcc ttccccgagg gctactcttg ggagcgcacc atgaccttcg
     3841 aggacaaggg catcgtgaag gtgaagtccg acatctccat ggaggaggac tccttcatct
     3901 acgagataca cctcaagggc gagaacttcc cccccaacgg ccccgtgatg cagaagaaga
     3961 ccaccggctg ggacgcctcc accgagagga tgtacgtgcg cgacggcgtg ctgaagggcg
     4021 acgtcaagca caagctgctg ctggagggcg gcggccacca ccgcgttgac ttcaagacca
     4081 tctacagggc caagaaggcg gtgaagctgc ccgactatca ctttgtggac caccgcatcg
     4141 agatcctgaa ccacgacaag gactacaaca aggtgaccgt ttacgagagc gccgtggccc
     4201 gcaactccac cgacggcatg gacgagctgt acaaggggcc aggtggtgca gggccaggtg
     4261 gtgcagggcc aggtggtgca gggccaggtg gtgcagggcc cggtggtgca ggtccaggtg
     4321 gtgcaggtcc aggtggtgca ggtccaggtg gtgctatggt gagcaagggc gaggagctgt
     4381 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     4441 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagctgatct
     4501 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg ggctacggcc
     4561 tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     4621 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     4681 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     4741 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     4801 acaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc
     4861 gccacaacat cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag aacaccccca
     4921 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag tccaaactga
     4981 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     5041 ggatcactct cggcatggac gagctgtaca aggcggccgc ggagttccca gagcagaaag
     5101 caggagaagc tattaatcag cccatgatga tggctgctag gcagttgcat gacgaggctc
     5161 ggaaatggtc tagcaagggt aacgacatca ttgctgctgc taaacgaatg gcgctgctca
     5221 tggctgagat gtcacgcctg gtgcgaggag gcagcggaaa caagcgtgcc ctcatccagt
     5281 gtgcaaaaga tattgctaag gcatcggatg aagtcactcg attggccaaa gaggtggcca
     5341 agcaatgtac tgataaacgc attagaacaa acctcttaca ggtctgtgag cgaatcccaa
     5401 ccatcagcac gcagctcaaa gctctttcca cagtgaaagc taccatgctg ggcaggacta
     5461 acatcagcga tgaagaatca gaacaggcaa ctgagatgtt ggttcataac gcccagaacc
     5521 tcatgcagtc tgtgaaggaa actgtgagag aagctgaagc agcatccatt aagataagaa
     5581 cagatgccgg attcactctg cgctgggtca gaaagacccc atggtatcag taatctagag
     5641 tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     5701 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     5761 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     5821 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     5881 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     5941 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     6001 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
     6061 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     6121 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     6181 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa
     6241 ttaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     6301 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     6361 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     6421 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     6481 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     6541 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     6601 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     6661 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     6721 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     6781 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     6841 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     6901 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     6961 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     7021 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     7081 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcgacgcg
     7141 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     7201 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     7261 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     7321 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     7381 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     7441 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     7501 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     7561 aattttaaca aaatattaac gtttacaatt tcccaggtgg cacttttcgg ggaaatgtgc
     7621 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     7681 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     7741 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     7801 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     7861 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     7921 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     7981 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     8041 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     8101 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     8161 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     8221 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     8281 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     8341 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     8401 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     8461 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     8521 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     8581 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     8641 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     8701 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     8761 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     8821 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     8881 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     8941 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     9001 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     9061 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     9121 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     9181 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     9241 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     9301 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     9361 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     9421 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     9481 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     9541 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     9601 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     9661 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     9721 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
     9781 aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc
     9841 ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc
     9901 atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg
     9961 tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg
    10021 cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc
    10081 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
    10141 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
    10201 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
    10261 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
    10321 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
    10381 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
    10441 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
    10501 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
    10561 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
    10621 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
    10681 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat
    10741 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
    10801 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
    10861 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
    10921 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
    10981 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
    11041 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
    11101 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
    11161 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
    11221 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
    11281 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
    11341 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
    11401 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
    11461 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
    11521 cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg
    11581 attagtgaac ggatctcgac gg
//
