LOCUS       sequence-21015-e         616 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 616)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG305GAL-ccdB-Cerulean
FEATURES             Location/Qualifiers
     source          1..616
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(83..207)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(284..305)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(545..564)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gacatcaaaa acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata
       61 cacagccagt ctgcaggtcg accatagtga ctggatatgt tgtgttttac agtattatgt
      121 agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc
      181 tcgttcagct ttcttgtaca aagtggtgat gggctgcagg aattcgatat caagcttaat
      241 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
      301 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
      361 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
      421 cgtgaccacc ctgacctggg gcgtgcagtg cttcgcccgc taccccgacc acatgaagca
      481 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
      541 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
      601 gaaccgcatc gagctg
//
