LOCUS       sequence-210151-       11602 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vinculin tension sensor with point mutation affecting ability to 
            bind talin in lentiviral expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11602)
  AUTHORS   Rothenberg KE, Scott DW, Christoforou N, Hoffman BD
  TITLE     Vinculin Force-Sensitive Dynamics at Focal Adhesions Enable 
            Effective Directed Cell Migration.
  JOURNAL   Biophys J. 2018 Apr 10;114(7):1680-1694. doi: 
            10.1016/j.bpj.2018.02.019.
  PUBMED    29642037
REFERENCE   2  (bases 1 to 11602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bpj.2018.02"; journalName: "Biophys J"; date: 
            "2018-04-10- 10"; volume: "114"; issue: "7"; pages: "1680-1694"
COMMENT     Sequence Label: pRRL-VinculinTS-A50I
FEATURES             Location/Qualifiers
     source          1..11602
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    31..147
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        207..586
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        587..790
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     741..761
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        835..853
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3547..4254
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     CDS             join(4375..4377,4378..4380,4381..5091)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4420..4441)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4681..4700)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5028..5049
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5665..6253
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5718..5738)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6136..6147)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6344..6577
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6649..6770
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6686..6705)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6740..6759
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6810..6945
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(6966..6984)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6994..7011)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6994..7010)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7003..7025)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7155..7610
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7242..7261)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7452..7473
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7639..7743
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7744..7812,7813..8604)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7962..7981)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8775..9363
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9264..9283
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9517..9534
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9651..9672
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9687..9692,9693..9710,9711..9717)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9725..9741
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9730..9752
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9749..9765
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9749..9765
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9784..9804
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9786..9804
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9832..10058
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             10059..10239
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10286..10411
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10904..11137
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11322..11366
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 agtgaacgga tctcgacggt atcggttaac ttttaaaaga aaagggggga ttggggggta
       61 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
      121 aaaacaaatt acaaaattca aaattttatc gatggatccc ccgggctcga gttcgaagat
      181 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
      241 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      301 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      361 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      421 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      481 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      541 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      601 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      661 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      721 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      781 aagcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac
      841 gactcactat agggagaccc aagctggcta gcgtttaaac ttaagcttgc catgcccgtc
      901 ttccacacgc gcaccatcga gagcatcttg gagcccgtgg ctcagcagat ctcccacctg
      961 gtcatcatgc acgaggaggg ggaggtagac ggcaaggcca tcccggacct caccgccccc
     1021 gtgtcggccg tgcaggccat cgtcagcaac ctggtgcggg ttggaaaaga aactgtgcag
     1081 acaacagaag accagatctt gaaaagggat atgccaccag cattcatcaa agtagagaat
     1141 gcctgcacca agctcgttcg agcagcccag atgctgcaag cagatcctta ttcagtacca
     1201 gctcgtgact acctaattga tggatcaaga ggcatccttt ctggaacatc agacttactt
     1261 ctgacatttg atgaagcgga ggtccgtaaa atcatccgtg tctgcaaagg aatattggaa
     1321 tatctgactg tggcagaagt agtagagact atggaggatt tggtgacata tacaaagaat
     1381 ctagggccag gaatgacaaa gatggcgaaa atgatcgatg agagacaaca ggaattaact
     1441 catcaggaac atagggttat gctggtgaac tccatgaata ctgtgaagga gctattgcct
     1501 gtacttattt cagctatgaa gatctttgta accacaaaaa acactaaaag ccagggaata
     1561 gaagaggcct tgaaaaatcg caatttcaca gtagagaaaa tgagtgctga gataaatgaa
     1621 ataatccgtg tattacaact cacttcctgg gatgaagatg cctgggccag caaggacact
     1681 gaagccatga aaagagcttt agccctaata gattcaaaga tgaaccaggc aaaaggctgg
     1741 ctgagagatc caaacgcacc tccaggggat gctggtgagc aagcaataag gcagatcctt
     1801 gatgaagctg gaaaagcagg agaattgtgt gcaggcaaag aacgcaggga gattctggga
     1861 acatgcaaaa ctttgggcca aatgactgat caacttgctg atctccgagc tagaggacag
     1921 ggtgctacac cgatggcaat gcagaaggca cagcaggtgt cacaaggcct ggatttgctc
     1981 actgcaaaag tggagaatgc agcccggaaa ttggaggcca tgacaaactc taagcaggct
     2041 attgcaaaga agattgatgc tgctcagaac tggcttgcgg atcccaacgg cggaagtgaa
     2101 ggagaagaac acattcgagg aattatgtct gaagcaagga aagttgcaga attgtgtgag
     2161 gagcctaaag aaagagatga tatccttcgc tccttggggg aaatctctgc tctgacagct
     2221 aagctgtcag atctgcgacg acatgggaaa ggcgactctc ctgaggcccg tgcattggcc
     2281 aagcaaatag ctacatcact tcagaactta cagtccaaaa caaacagagc tgtagcaaat
     2341 actaggccag ttaaagctgc tgtccatttg gagggcaaga ttgagcaagc ccagaggtgg
     2401 atagataatc ctacagttga tgatcgggga gtaggccagg cagcaattcg gggtttggtt
     2461 gcagaaggtc gtcgtctagc caatgtcatg atgggacctt atcgtcagga cctgcttgcc
     2521 aaatgtgacc gtgtagacca gctggctgct cagcttgctg accttgcagc aagaggggaa
     2581 ggagagtctc ctcaggctag ggcaattgct gctcagcttc aggactccct gaaggatctc
     2641 aaagcacgga tgcaagaagc aatgacccag gaggtgtctg atgttttcag tgacactaca
     2701 acccctatta agttgttagc agtagcagcc actgctcctt ctgatactcc caatagagaa
     2761 gaggtgtttg aagaaagagc agcaaatttt gaaaaccatg ctgctagact gggagcaaca
     2821 gcagaaaaag cagctgcagt tggaactgct aataaaacta ctgtggaagg cattcaggca
     2881 acagtcaaat ctgcaaggga gcttacacca caggtagtat cggctgctcg aatcctcctg
     2941 agaaatcctg gaaatcaagc tgcttatgag cattttgaga caatgaaaaa ccaatggatt
     3001 gataatgtag aaaagatgac agggctggtg gatgaggcca tcgataccaa gtctctgttg
     3061 gatgcatcag aagaggctat taagaaggat cttgataaat gtaaagttgc aatggccaat
     3121 atgcaacctc agatgctggt agctggagcc accagcattg ctagacgagc gaaccgcatc
     3181 ctgcttgtgg caaaacggga ggttgaaaat tcagaagacc ctaaattcag ggaggctgtt
     3241 aaagcagctt ctgatgagct gagcaaaacc atatcaccga tggtaatgga tgctaaagct
     3301 gtagcaggaa atatctctga tcctggtttg cagaagagtt tcttggattc tggatacagg
     3361 attctgggag ctgtggccaa agtcagagaa gcctttcagc ctcaggagcc agactttccc
     3421 cctcctcctc ctgacctaga gcatctccat ctgactgatg agcttgctcc tccaaaacca
     3481 ccacttccag aaggtgaggt tcccccaccc agaccaccac cccctgaaga aaaagatgag
     3541 gtcgagatgg tgagcaaggg cgaggagacc acaatgggcg taatcaagcc cgacatgaag
     3601 atcaagctga agatggaggg caacgtgaat ggccacgcct tcgtgatcga gggcgagggc
     3661 gagggcaagc cctacgacgg caccaacacc atcaacctgg aggtgaagga gggagccccc
     3721 ctgcccttct cctacgacat tctgaccacc gcgttcgcct acggcaacag ggccttcacc
     3781 aagtaccccg acgacatccc caactacttc aagcagtcct tccccgaggg ctactcttgg
     3841 gagcgcacca tgaccttcga ggacaagggc atcgtgaagg tgaagtccga catctccatg
     3901 gaggaggact ccttcatcta cgagatacac ctcaagggcg agaacttccc ccccaacggc
     3961 cccgtgatgc agaagaagac caccggctgg gacgcctcca ccgagaggat gtacgtgcgc
     4021 gacggcgtgc tgaagggcga cgtcaagcac aagctgctgc tggagggcgg cggccaccac
     4081 cgcgttgact tcaagaccat ctacagggcc aagaaggcgg tgaagctgcc cgactatcac
     4141 tttgtggacc accgcatcga gatcctgaac cacgacaagg actacaacaa ggtgaccgtt
     4201 tacgagagcg ccgtggcccg caactccacc gacggcatgg acgagctgta caaggggcca
     4261 ggtggtgcag ggccaggtgg tgcagggcca ggtggtgcag ggccaggtgg tgcagggccc
     4321 ggtggtgcag gtccaggtgg tgcaggtcca ggtggtgcag gtccaggtgg tgctatggtg
     4381 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     4441 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     4501 ctgaccctga agctgatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     4561 accaccctgg gctacggcct gcagtgcttc gcccgctacc ccgaccacat gaagcagcac
     4621 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     4681 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     4741 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     4801 gagtacaact acaacagcca caacgtctat atcaccgccg acaagcagaa gaacggcatc
     4861 aaggccaact tcaagatccg ccacaacatc gaggacggcg gcgtgcagct cgccgaccac
     4921 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     4981 agctaccagt ccaaactgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     5041 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa ggcggccgcg
     5101 gagttcccag agcagaaagc aggagaagct attaatcagc ccatgatgat ggctgctagg
     5161 cagttgcatg acgaggctcg gaaatggtct agcaagggta acgacatcat tgctgctgct
     5221 aaacgaatgg cgctgctcat ggctgagatg tcacgcctgg tgcgaggagg cagcggaaac
     5281 aagcgtgccc tcatccagtg tgcaaaagat attgctaagg catcggatga agtcactcga
     5341 ttggccaaag aggtggccaa gcaatgtact gataaacgca ttagaacaaa cctcttacag
     5401 gtctgtgagc gaatcccaac catcagcacg cagctcaaaa ttctttccac agtgaaagct
     5461 accatgctgg gcaggactaa catcagcgat gaagaatcag aacaggcaac tgagatgttg
     5521 gttcataacg cccagaacct catgcagtct gtgaaggaaa ctgtgagaga agctgaagca
     5581 gcatccatta agataagaac agatgccgga ttcactctgc gctgggtcag aaagacccca
     5641 tggtatcagt aagctagagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg
     5701 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     5761 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     5821 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     5881 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     5941 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     6001 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag
     6061 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     6121 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     6181 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     6241 gccgcctccc cgcctggaat taattcgagc tcggtacctt taagaccaat gacttacaag
     6301 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     6361 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     6421 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     6481 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     6541 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     6601 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
     6661 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     6721 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     6781 ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     6841 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     6901 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa
     6961 ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga
     7021 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     7081 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     7141 tggcgaatgg cgcgacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     7201 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     7261 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     7321 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     7381 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     7441 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     7501 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     7561 ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt cccaggtggc
     7621 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     7681 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     7741 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     7801 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     7861 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     7921 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     7981 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     8041 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     8101 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     8161 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     8221 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     8281 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     8341 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     8401 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     8461 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     8521 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     8581 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     8641 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     8701 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     8761 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     8821 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     8881 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     8941 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     9001 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     9061 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     9121 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     9181 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     9241 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     9301 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     9361 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     9421 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     9481 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     9541 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     9601 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     9661 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     9721 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     9781 cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt
     9841 atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag
     9901 gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat
     9961 taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca
    10021 gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga
    10081 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
    10141 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
    10201 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
    10261 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
    10321 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
    10381 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
    10441 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
    10501 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
    10561 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
    10621 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
    10681 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
    10741 acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
    10801 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
    10861 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
    10921 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
    10981 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
    11041 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
    11101 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
    11161 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
    11221 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
    11281 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
    11341 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
    11401 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
    11461 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
    11521 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
    11581 acagagacag atccattcga tt
//
