LOCUS       sequence-210154-       10118 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vinculin tension sensor with point mutations affecting ability to 
            bind talin and actin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10118)
  AUTHORS   Rothenberg KE, Scott DW, Christoforou N, Hoffman BD
  TITLE     Vinculin Force-Sensitive Dynamics at Focal Adhesions Enable 
            Effective Directed Cell Migration.
  JOURNAL   Biophys J. 2018 Apr 10;114(7):1680-1694. doi: 
            10.1016/j.bpj.2018.02.019.
  PUBMED    29642037
REFERENCE   2  (bases 1 to 10118)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bpj.2018.02"; journalName: "Biophys J"; date: 
            "2018-04-10- 10"; volume: "114"; issue: "7"; pages: "1680-1694"
COMMENT     Sequence Label: VinculinTS-A50I/I997A
FEATURES             Location/Qualifiers
     source          1..10118
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3575..4282
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     CDS             join(4403..4405,4406..4408,4409..5119)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4448..4469)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4709..4728)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5056..5077
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5712..5729)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5718..5942
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5988..6416
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6075..6094)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6285..6306
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6425..6445)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6430..6759
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6610..6745
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6672..6691
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6826..7620
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6880..6899)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7490..7509
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7794..7915
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7831..7850)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7885..7904
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7964..7980)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7964..7980)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7977..7999)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7988..8004
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8012..8018,8019..8036,8037..8042))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8057..8078
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8195..8212)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8366..8951)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8446..8465)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9122..9913,9914..9982))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9745..9764
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9983..10087)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttgcca tgcccgtctt ccacacgcgc accatcgaga gcatcttgga
      961 gcccgtggct cagcagatct cccacctggt catcatgcac gaggaggggg aggtagacgg
     1021 caaggccatc ccggacctca ccgcccccgt gtcggccgtg caggccatcg tcagcaacct
     1081 ggtgcgggtt ggaaaagaaa ctgtgcagac aacagaagac cagatcttga aaagggatat
     1141 gccaccagca ttcatcaaag tagagaatgc ctgcaccaag ctcgttcgag cagcccagat
     1201 gctgcaagca gatccttatt cagtaccagc tcgtgactac ctaattgatg gatcaagagg
     1261 catcctttct ggaacatcag acttacttct gacatttgat gaagcggagg tccgtaaaat
     1321 catccgtgtc tgcaaaggaa tattggaata tctgactgtg gcagaagtag tagagactat
     1381 ggaggatttg gtgacatata caaagaatct agggccagga atgacaaaga tggcgaaaat
     1441 gatcgatgag agacaacagg aattaactca tcaggaacat agggttatgc tggtgaactc
     1501 catgaatact gtgaaggagc tattgcctgt acttatttca gctatgaaga tctttgtaac
     1561 cacaaaaaac actaaaagcc agggaataga agaggccttg aaaaatcgca atttcacagt
     1621 agagaaaatg agtgctgaga taaatgaaat aatccgtgta ttacaactca cttcctggga
     1681 tgaagatgcc tgggccagca aggacactga agccatgaaa agagctttag ccctaataga
     1741 ttcaaagatg aaccaggcaa aaggctggct gagagatcca aacgcacctc caggggatgc
     1801 tggtgagcaa gcaataaggc agatccttga tgaagctgga aaagcaggag aattgtgtgc
     1861 aggcaaagaa cgcagggaga ttctgggaac atgcaaaact ttgggccaaa tgactgatca
     1921 acttgctgat ctccgagcta gaggacaggg tgctacaccg atggcaatgc agaaggcaca
     1981 gcaggtgtca caaggcctgg atttgctcac tgcaaaagtg gagaatgcag cccggaaatt
     2041 ggaggccatg acaaactcta agcaggctat tgcaaagaag attgatgctg ctcagaactg
     2101 gcttgcggat cccaacggcg gaagtgaagg agaagaacac attcgaggaa ttatgtctga
     2161 agcaaggaaa gttgcagaat tgtgtgagga gcctaaagaa agagatgata tccttcgctc
     2221 cttgggggaa atctctgctc tgacagctaa gctgtcagat ctgcgacgac atgggaaagg
     2281 cgactctcct gaggcccgtg cattggccaa gcaaatagct acatcacttc agaacttaca
     2341 gtccaaaaca aacagagctg tagcaaatac taggccagtt aaagctgctg tccatttgga
     2401 gggcaagatt gagcaagccc agaggtggat agataatcct acagttgatg atcggggagt
     2461 aggccaggca gcaattcggg gtttggttgc agaaggtcgt cgtctagcca atgtcatgat
     2521 gggaccttat cgtcaggacc tgcttgccaa atgtgaccgt gtagaccagc tggctgctca
     2581 gcttgctgac cttgcagcaa gaggggaagg agagtctcct caggctaggg caattgctgc
     2641 tcagcttcag gactccctga aggatctcaa agcacggatg caagaagcaa tgacccagga
     2701 ggtgtctgat gttttcagtg acactacaac ccctattaag ttgttagcag tagcagccac
     2761 tgctccttct gatactccca atagagaaga ggtgtttgaa gaaagagcag caaattttga
     2821 aaaccatgct gctagactgg gagcaacagc agaaaaagca gctgcagttg gaactgctaa
     2881 taaaactact gtggaaggca ttcaggcaac agtcaaatct gcaagggagc ttacaccaca
     2941 ggtagtatcg gctgctcgaa tcctcctgag aaatcctgga aatcaagctg cttatgagca
     3001 ttttgagaca atgaaaaacc aatggattga taatgtagaa aagatgacag ggctggtgga
     3061 tgaggccatc gataccaagt ctctgttgga tgcatcagaa gaggctatta agaaggatct
     3121 tgataaatgt aaagttgcaa tggccaatat gcaacctcag atgctggtag ctggagccac
     3181 cagcattgct agacgagcga accgcatcct gcttgtggca aaacgggagg ttgaaaattc
     3241 agaagaccct aaattcaggg aggctgttaa agcagcttct gatgagctga gcaaaaccat
     3301 atcaccgatg gtaatggatg ctaaagctgt agcaggaaat atctctgatc ctggtttgca
     3361 gaagagtttc ttggattctg gatacaggat tctgggagct gtggccaaag tcagagaagc
     3421 ctttcagcct caggagccag actttccccc tcctcctcct gacctagagc atctccatct
     3481 gactgatgag cttgctcctc caaaaccacc acttccagaa ggtgaggttc ccccacccag
     3541 accaccaccc cctgaagaaa aagatgaggt cgagatggtg agcaagggcg aggagaccac
     3601 aatgggcgta atcaagcccg acatgaagat caagctgaag atggagggca acgtgaatgg
     3661 ccacgccttc gtgatcgagg gcgagggcga gggcaagccc tacgacggca ccaacaccat
     3721 caacctggag gtgaaggagg gagcccccct gcccttctcc tacgacattc tgaccaccgc
     3781 gttcgcctac ggcaacaggg ccttcaccaa gtaccccgac gacatcccca actacttcaa
     3841 gcagtccttc cccgagggct actcttggga gcgcaccatg accttcgagg acaagggcat
     3901 cgtgaaggtg aagtccgaca tctccatgga ggaggactcc ttcatctacg agatacacct
     3961 caagggcgag aacttccccc ccaacggccc cgtgatgcag aagaagacca ccggctggga
     4021 cgcctccacc gagaggatgt acgtgcgcga cggcgtgctg aagggcgacg tcaagcacaa
     4081 gctgctgctg gagggcggcg gccaccaccg cgttgacttc aagaccatct acagggccaa
     4141 gaaggcggtg aagctgcccg actatcactt tgtggaccac cgcatcgaga tcctgaacca
     4201 cgacaaggac tacaacaagg tgaccgttta cgagagcgcc gtggcccgca actccaccga
     4261 cggcatggac gagctgtaca aggggccagg tggtgcaggg ccaggtggtg cagggccagg
     4321 tggtgcaggg ccaggtggtg cagggcccgg tggtgcaggt ccaggtggtg caggtccagg
     4381 tggtgcaggt ccaggtggtg ctatggtgag caagggcgag gagctgttca ccggggtggt
     4441 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     4501 gggcgagggc gatgccacct acggcaagct gaccctgaag ctgatctgca ccaccggcaa
     4561 gctgcccgtg ccctggccca ccctcgtgac caccctgggc tacggcctgc agtgcttcgc
     4621 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     4681 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     4741 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     4801 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     4861 caccgccgac aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga
     4921 ggacggcggc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     4981 cgtgctgctg cccgacaacc actacctgag ctaccagtcc aagctgagca aagaccccaa
     5041 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     5101 catggacgag ctgtacaagg cggccgcgga gttcccagag cagaaagcag gagaagctat
     5161 taatcagccc atgatgatgg ctgctaggca gttgcatgac gaggctcgga aatggtctag
     5221 caagggtaac gacatcattg ctgctgctaa acgaatggcg ctgctcatgg ctgagatgtc
     5281 acgcctggtg cgaggaggca gcggaaacaa gcgtgccctc atccagtgtg caaaagatat
     5341 tgctaaggca tcggatgaag tcactcgatt ggccaaagag gtggccaagc aatgtactga
     5401 taaacgcatt agaacaaacc tcttacaggt ctgtgagcga atcccaacca tcagcacgca
     5461 gctcaaagct ctttccacag tgaaagctac catgctgggc aggactaaca tcagcgatga
     5521 agaatcagaa caggcaactg agatgttggt tcataacgcc cagaacctca tgcagtctgt
     5581 gaaggaaact gtgagagaag ctgaagcagc atccattaag ataagaacag atgccggatt
     5641 cactctgcgc tgggtcagaa agaccccatg gtatcagtaa tctagagggc ccgtttaaac
     5701 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     5761 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     5821 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     5881 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     5941 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     6001 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     6061 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     6121 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     6181 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     6241 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     6301 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     6361 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
     6421 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     6481 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     6541 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     6601 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     6661 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     6721 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
     6781 tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
     6841 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     6901 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     6961 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
     7021 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     7081 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     7141 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     7201 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     7261 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     7321 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
     7381 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     7441 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     7501 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     7561 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     7621 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
     7681 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     7741 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
     7801 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     7861 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     7921 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     7981 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     8041 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     8101 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     8161 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     8221 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     8281 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     8341 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     8401 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     8461 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     8521 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     8581 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     8641 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     8701 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     8761 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     8821 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     8881 aacaaaccac cgctggtagc ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     8941 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     9001 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     9061 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     9121 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     9181 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     9241 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     9301 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     9361 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     9421 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     9481 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     9541 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     9601 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     9661 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     9721 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     9781 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     9841 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     9901 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     9961 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
    10021 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
    10081 ttccgcgcac atttccccga aaagtgccac ctgacgtc
//
