LOCUS       sequence-21020-e         614 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG306GAL-ccdB-Cerulean
FEATURES             Location/Qualifiers
     source          1..614
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(8..132)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(140..156)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(140..156)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(171..612)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(177..200)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    495..612
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
ORIGIN
        1 cggccgccat agtgactgga tatgttgtgt tttacagtat tatgtagtct gttttttatg
       61 caaaatctaa tttaatatat tgatatttat atcattttac gtttctcgtt cagctttttt
      121 gtacaaactt gtgatggggg atccactagt tctagaatcc ggggtttttt ctccttgacg
      181 ttaaagtata gaggtatatt aacaattttt tgttgatact tttattacat ttgaataaga
      241 agtaatacaa accgaaaatg ttgaaagtat tagttaaagt ggttatgcag tttttgcatt
      301 tatatatctg ttaatagatc aaaaatcatc gcttcgctga ttaattaccc cagaaataag
      361 gctaaaaaac taatcgcatt atcatcctat ggttgttaat ttgattcgtt catttgaagg
      421 tttgtggggc caggttactg ccaatttttc ctcttcataa ccataaaagc tagtattgta
      481 gaatctttat tgttcggagc agtgcggcgc gaggcacatc tgcgtttcag gaacgcgacc
      541 ggtgaagacg aggacgcacg gaggagagtc ttccttcgga gggctgtcac ccgctcggcg
      601 gcttctaatc cgta
//
