LOCUS       sequence-210214-        9147 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry plasmid (attL1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9147)
  AUTHORS   Lee K, Zhang Y, Kleinstiver BP, Guo JA, Aryee MJ, Miller J, Malzahn 
            A, Zarecor S, Lawrence-Dill CJ, Joung JK, Qi Y, Wang K
  TITLE     Activities and specificities of CRISPR/Cas9 and Cas12a nucleases for
            targeted mutagenesis in maize.
  JOURNAL   Plant Biotechnol J. 2018 Jul 4. doi: 10.1111/pbi.12982.
  PUBMED    29972722
REFERENCE   2  (bases 1 to 9147)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2018 Jul 4. doi: 10.1111/pbi.12982."
COMMENT     Sequence Label: pYPQ165
FEATURES             Location/Qualifiers
     source          1..9147
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             358..1149
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1242..1830
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1731..1750
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1984..2001
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2160..2187)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2279..2365)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2414..2436
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2428..2445
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2429..2445
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2461..2560
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3954..4019
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             4026..4046
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4071..8171
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4071..8171
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             8084..8092
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             8172..8219
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     protein_bind    8894..9017
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(9034..9053)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9035..9053)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9058..9074)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9058..9074)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tggaccagcc aggacagaaa tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc
       61 acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag
      121 ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
      181 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag
      241 tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta
      301 tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacattatg
      361 agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag
      421 cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc
      481 ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca
      541 acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag
      601 attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat
      661 ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc
      721 ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat
      781 agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat
      841 ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc
      901 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa
      961 atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag
     1021 cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg
     1081 cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc
     1141 ggcaaataac cctcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc
     1201 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     1261 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     1321 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     1381 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     1441 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     1501 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     1561 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     1621 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     1681 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     1741 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     1801 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     1861 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     1921 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     1981 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     2041 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     2101 agtgagcgca acgcaattaa tacgcgtacc gcgagccagg aagagtttgt agaaacgcaa
     2161 aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc
     2221 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat
     2281 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     2341 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgctt
     2401 gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc
     2461 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
     2521 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgaattcgc ccttcaccat
     2581 ggttatacta gtgaataaaa gcatttgcgt ttggtttatc attgcgttta tacaaggaca
     2641 gagatccact gagctggaat agcttaaaac cattatcaga acaaaataaa ccattttttg
     2701 ttaagaatca gagcatagta aacaacagaa acaacctaag agaggtaact tgtccaagaa
     2761 gatagctaat tatatctatt ttataaaagt tatcatagtt tgtaagtcac aaaagatgca
     2821 aataacagag aaactaggag acttgagaat atacattctt gtatatttgt attcgagatt
     2881 gtgaaaattt gaccataagt ttaaattctt aaaaagatat atctgatcta ggtgatggtt
     2941 atagactgta attttaccac atgtttaatg atggatagtg acacacatga cacatcgaca
     3001 acactatagc atcttattta gattacaaca tgaaattttt ctgtaataca tgtctttgta
     3061 cataatttaa aagtaattcc taagaaatat atttatacaa ggagtttaaa gaaaacatag
     3121 cataaagttc aatgagtagt aaaaaccata tacagtatat agcataaagt tcaatgagtt
     3181 tattacaaaa gcattggttc actttctgta acacgacgtt aaaccttcgt ctccaatagg
     3241 agcgctactg attcaacatg ccaatatata ctaaatacgt ttctacagtc aaatgcttta
     3301 acgtttcatg attaagtgac tatttaccgt caatcctttc ccattcctcc cactaatcca
     3361 actttttaat tactcttaaa tcaccactaa gctagtaacg cctatcatga attagctcta
     3421 ctaaatctag caacctttca aatttgcagt attgcaggtg tctctgtgtc tttaaaatag
     3481 ttgccttatg atttcttcgg tttcaagatg atcaaatagt tatagatttc atgctcacac
     3541 atgctcatta gatgtgtaca tactttactt acccaaatct attttctcgc aaagattttg
     3601 atggtaaagc tgatttggtt ctattgaact aaatcaaacg agtttcagac tgagtgattc
     3661 taatccggcc cattagcccc taaacagacc cactaattac gcagctttta atagagtaat
     3721 tacacctagt ttacccacta aaccactaag cactaattat ctcacaatct aatgagcttc
     3781 cctcgtaatt acttgggctt tcactctacc atttatttgt aacagtcaag tctctactgt
     3841 ctctatataa actctctaaa gttaacacac aattctcatc acaaacaaat caaccaaagc
     3901 aacttctact ctttcttctt tcgaccttat caatctgttg agaaatctag atggattaca
     3961 aggaccacga cggggattac aaggaccacg acattgatta caaggatgat gatgacaaga
     4021 tggctccgaa gaagaagagg aaggttggca tccacggggt gccagctgct gacaagaagt
     4081 actcgatcgg cctcgatatt gggactaact ctgttggctg ggccgtgatc accgacgagt
     4141 acaaggtgcc ctcaaagaag ttcaaggtcc tgggcaacac cgatcggcat tccatcaaga
     4201 agaatctcat tggcgctctc ctgttcgaca gcggcgagac ggctgaggct acgcggctca
     4261 agcgcaccgc ccgcaggcgg tacacgcgca ggaagaatcg catctgctac ctgcaggaga
     4321 ttttctccaa cgagatggcg aaggttgacg attctttctt ccacaggctg gaggagtcat
     4381 tcctcgtgga ggaggataag aagcacgagc ggcatccaat cttcggcaac attgtcgacg
     4441 aggttgccta ccacgagaag taccctacga tctaccatct gcggaagaag ctcgtggact
     4501 ccacagataa ggcggacctc cgcctgatct acctcgctct ggcccacatg attaagttca
     4561 ggggccattt cctgatcgag ggggatctca acccggacaa tagcgatgtt gacaagctgt
     4621 tcatccagct cgtgcagacg tacaaccagc tcttcgagga gaaccccatt aatgcgtcag
     4681 gcgtcgacgc gaaggctatc ctgtccgcta ggctctcgaa gtctcggcgc ctcgagaacc
     4741 tgatcgccca gctgccgggc gagaagaaga acggcctgtt cgggaatctc attgcgctca
     4801 gcctggggct cacgcccaac ttcaagtcga atttcgatct cgctgaggac gccaagctgc
     4861 agctctccaa ggacacatac gacgatgacc tggataacct cctggcccag atcggcgatc
     4921 agtacgcgga cctgttcctc gctgccaaga atctgtcgga cgccatcctc ctgtctgata
     4981 ttctcagggt gaacaccgag attacgaagg ctccgctctc agcctccatg atcaagcgct
     5041 acgacgagca ccatcaggat ctgaccctcc tgaaggcgct ggtcaggcag cagctccccg
     5101 agaagtacaa ggagatcttc ttcgatcagt cgaagaacgg ctacgctggg tacattgacg
     5161 gcggggcctc tcaggaggag ttctacaagt tcatcaagcc gattctggag aagatggacg
     5221 gcacggagga gctgctggtg aagctcaatc gcgaggacct cctgaggaag cagcggacat
     5281 tcgataacgg cagcatccca caccagattc atctcgggga gctgcacgct atcctgagga
     5341 ggcaggagga cttctaccct ttcctcaagg ataaccgcga gaagatcgag aagattctga
     5401 ctttcaggat cccgtactac gtcggcccac tcgctagggg caactcccgc ttcgcttgga
     5461 tgacccgcaa gtcagaggag acgatcacgc cgtggaactt cgaggaggtg gtcgacaagg
     5521 gcgctagcgc tcagtcgttc atcgagagga tgacgaattt cgacaagaac ctgccaaatg
     5581 agaaggtgct ccctaagcac tcgctcctgt acgagtactt cacagtctac aacgagctga
     5641 ctaaggtgaa gtatgtgacc gagggcatga ggaagccggc tttcctgtct ggggagcaga
     5701 agaaggccat cgtggacctc ctgttcaaga ccaaccggaa ggtcacggtt aagcagctca
     5761 aggaggacta cttcaagaag attgagtgct tcgattcggt cgagatctct ggcgttgagg
     5821 accgcttcaa cgcctccctg gggacctacc acgatctcct gaagatcatt aaggataagg
     5881 acttcctgga caacgaggag aatgaggata tcctcgagga cattgtgctg acactcactc
     5941 tgttcgagga ccgggagatg atcgaggagc gcctgaagac ttacgcccat ctcttcgatg
     6001 acaaggtcat gaagcagctc aagaggagga ggtacaccgg ctgggggagg ctgagcagga
     6061 agctcatcaa cggcattcgg gacaagcagt ccgggaagac gatcctcgac ttcctgaaga
     6121 gcgatggctt cgcgaaccgc aatttcatgc agctgattca cgatgacagc ctcacattca
     6181 aggaggatat ccagaaggct caggtgagcg gccaggggga ctcgctgcac gagcatatcg
     6241 cgaacctcgc tggctcgcca gctatcaaga aggggattct gcagaccgtg aaggttgtgg
     6301 acgagctggt gaaggtcatg ggcaggcaca agcctgagaa catcgtcatt gagatggccc
     6361 gggagaatca gaccacgcag aagggccaga agaactcacg cgagaggatg aagaggatcg
     6421 aggagggcat taaggagctg gggtcccaga tcctcaagga gcacccggtg gagaacacgc
     6481 agctgcagaa tgagaagctc tacctgtact acctccagaa tggccgcgat atgtatgtgg
     6541 accaggagct ggatattaac aggctcagcg attacgacgt cgatcatatc gttccacagt
     6601 cattcctgaa ggatgactcc attgacaaca aggtcctcac caggtcggac aagaaccggg
     6661 gcaagtctga taatgttcct tcagaggagg tcgttaagaa gatgaagaac tactggcgcc
     6721 agctcctgaa tgccaagctg atcacgcagc ggaagttcga taacctcaca aaggctgaga
     6781 ggggcgggct ctctgagctg gacaaggcgg gcttcatcaa gaggcagctg gtcgagacac
     6841 ggcagatcac taagcacgtt gcgcagattc tcgactcacg gatgaacact aagtacgatg
     6901 agaatgacaa gctgatccgc gaggtgaagg tcatcaccct gaagtcaaag ctcgtctccg
     6961 acttcaggaa ggatttccag ttctacaagg ttcgggagat caacaattac caccatgccc
     7021 atgacgcgta cctgaacgcg gtggtcggca cagctctgat caagaagtac ccaaagctcg
     7081 agagcgagtt cgtgtacggg gactacaagg tttacgatgt gaggaagatg atcgccaagt
     7141 cggagcagga gattggcaag gctaccgcca agtacttctt ctactctaac attatgaatt
     7201 tcttcaagac agagatcact ctggccaatg gcgagatccg gaagcgcccc ctcatcgaga
     7261 cgaacggcga gacgggggag atcgtgtggg acaagggcag ggatttcgcg accgtcagga
     7321 aggttctctc catgccacaa gtgaatatcg tcaagaagac agaggtccag actggcgggt
     7381 tctctaagga gtcaattctg cctaagcgga acagcgacaa gctcatcgcc cgcaagaagg
     7441 actgggatcc gaagaagtac ggcgggttcg acagccccac tgtggcctac tcggtcctgg
     7501 ttgtggcgaa ggttgagaag ggcaagtcca agaagctcaa gagcgtgaag gagctgctgg
     7561 ggatcacgat tatggagcgc tccagcttcg agaagaaccc gatcgatttc ctggaggcga
     7621 agggctacaa ggaggtgaag aaggacctga tcattaagct ccccaagtac tcactcttcg
     7681 agctggagaa cggcaggaag cggatgctgg cttccgctgg cgagctgcag aaggggaacg
     7741 agctggctct gccgtccaag tatgtgaact tcctctacct ggcctcccac tacgagaagc
     7801 tcaagggcag ccccgaggac aacgagcaga agcagctgtt cgtcgagcag cacaagcatt
     7861 acctcgacga gatcattgag cagatttccg agttctccaa gcgcgtgatc ctggccgacg
     7921 cgaatctgga taaggtcctc tccgcgtaca acaagcaccg cgacaagcca atcagggagc
     7981 aggctgagaa tatcattcat ctcttcaccc tgacgaacct cggcgcccct gctgctttca
     8041 agtacttcga cacaactatc gatcgcaaga ggtacacaag cactaaggag gtcctggacg
     8101 cgaccctcat ccaccagtcg attaccggcc tctacgagac gcgcatcgac ctgtctcagc
     8161 tcgggggcga caagcggcca gcggcgacga agaaggcggg gcaggcgaag aagaagaagt
     8221 gagctcagag ctttcgttcg tatcatcggt ttcgacaacg ttcgtcaagt tcaatgcatc
     8281 agtttcattg cgcacacacc agaatcctac tgagtttgag tattatggca ttgggaaaac
     8341 tgtttttctt gtaccatttg ttgtgcttgt aatttactgt gttttttatt cggttttcgc
     8401 tatcgaactg tgaaatggaa atggatggag aagagttaat gaatgatatg gtccttttgt
     8461 tcattctcaa attaatatta tttgtttttt ctcttatttg ttgtgtgttg aatttgaaat
     8521 tataagagat atgcaaacat tttgttttga gtaaaaatgt gtcaaatcgt ggcctctaat
     8581 gaccgaagtt aatatgagga gtaaaacact tgtagttgta ccattatgct tattcactag
     8641 gcaacaaata tattttcaga cctagaaaag ctgcaaatgt tactgaatac aagtatgtcc
     8701 tcttgtgttt tagacattta tgaactttcc tttatgtaat tttccagaat ccttgtcaga
     8761 ttctaatcat tgctttataa ttatagttat actcatggat ttgtagttga gtatgaaaat
     8821 attttttaat gcattttatg acttgccaat tgttccggaa ctctagataa gcttaccgga
     8881 aagggcgaat tcgcaacttt gtatacaaaa gttgaacgag aaacgtaaaa tgatataaat
     8941 atcaatatat taaattagat tttgcataaa aaacagacta cataatactg taaaacacaa
     9001 catatccagt cactatgcca tccagctgat atcccctata gtgagtcgta ttacatggtc
     9061 atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa
     9121 gataaaaata tatcatcatg cctcctc
//
