LOCUS       sequence-210224-        5061 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Parental vector for making S. rosetta expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Booth DS, Szmidt-Middleton H, King N
  TITLE     Transfection of choanoflagellates illuminates their cell biology and
            the ancestry of animal septins.
  JOURNAL   Mol Biol Cell. 2018 Dec 1;29(25):3026-3038. doi: 
            10.1091/mbc.E18-08-0514. Epub 2018 Oct 3.
  PUBMED    30281390
REFERENCE   2  (bases 1 to 5061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E18-08-0514"; journalName: "Mol Biol Cell"; date: 
            "2018-12-1- 1"; volume: "29"; issue: "25"; pages: "3026-3038"
COMMENT     Sequence Label: NK601
FEATURES             Location/Qualifiers
     source          1..5061
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(13..31)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        99..203
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(204..272,273..1064)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(422..441)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1235..1823
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1724..1743
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1977..1994
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2111..2132
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2147..2152,2153..2170,2171..2177)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2185..2201
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2190..2212
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2209..2225
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2209..2225
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4670..4687)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4670..4686)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4679..4701)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4895..4914)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5014..5036
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
       61 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
      121 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
      181 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
      241 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
      301 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
      361 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
      421 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
      481 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
      541 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
      601 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
      661 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
      721 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
      781 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
      841 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
      901 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
      961 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     1021 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     1081 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     1141 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     1201 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     1261 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     1321 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     1381 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     1441 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     1501 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     1561 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     1621 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     1681 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     1741 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     1801 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     1861 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     1921 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     1981 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     2041 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     2101 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     2161 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     2221 atgaccatga ttacgccaag cttgcatgcc tgcaggtaac tcatccttgt cagcatcgtt
     2281 tcctcacctc ctcctcctgt cgctggtgct tctggtcttg tccagcggga acccaaacaa
     2341 caacaacaac gaactaccac tgcctcggaa aatctgcggt gatttgcaca aatcgccaac
     2401 aagtgttgtg gcgtgctaaa tgagagaaac accgtatttg agcccaggta tcgaaatgtc
     2461 tgtatggctg catttgtgtt ttccaacctg aaggaaaaca gcacttgaca acgacgtttg
     2521 cccagcccgt ttttcgcggt ggatacaaat ttcacctgtg tcactgcgca ttagccccac
     2581 agtggggctt acgaaatcta caccatccca gcttccgcac cgccaaaatt aactgcgtga
     2641 aaaaacactt gcggcaacca cagcaaccac cacaagcagc caaacactcg caaacaccct
     2701 tgcttgcttc ttggcttttt tgtagtcccc tttcccttgt tttcctgttg tcgtggctga
     2761 ggtttgcgtg gcgtgttcat tttcatcagt ttgctcgctc ataacaagct tgcgactacc
     2821 tccccttttt ggtgctgtac acccgcgacc tttcttatcc tcttctcctt ctccaccacc
     2881 gctcaacgac aagatgcgcg aagttctctc cgtccacatt ggccaggccg gttgccagat
     2941 cggtaacgcc tgctgggagc tgtactgcct cgagcacggc atccaacccg atggccagat
     3001 gccctctgac aagactgtcg gctatggcga cgactcgttc aacaccttct tctccgagac
     3061 tggtgctggc aagcacgtgc cccgcgccgt gtttgtcgat ctcgagccca ccgtcgtcga
     3121 cgaggtgcgc accggcacct accgccagct gttccacccc gagcagctca tctccggcaa
     3181 ggaggacgcc gccaacaact acgcccgtgg ccactacacc gttggcaagg agctcgtcga
     3241 cattgtcctc gaccgcatcc gcaagctcgc cgaccagtgc accggtctcc agggcttcct
     3301 catcttccac tcgttcggtg gcggtaccgg ctctggcttt gcctcgctcc tcatggagcg
     3361 cctctctgtc gactacggca agaagtccaa gctcgagttt gccgtctacc ctgctcctca
     3421 ggtgtccacc gccgttgtcg agccctacaa ctcgatcctg acgtgccaca ccaccctcga
     3481 gcactctgac tgcgccttcc tcatggacaa cgaggccatc tacgacattt gccgtcgcaa
     3541 cctcgacatt gagcgcccca cctacaccaa cctgaaccgc ctcatcgccc aggttgtgtc
     3601 ctccgtgacg gcctccctgc gcttcgacgg tgcccttaac gtcgatctca ccgagttcca
     3661 gaccaacctg gtgccctacc cccgcatcca cttccctctg gtgacgtacg ctcccatcat
     3721 ctctgccgag aaggcctacc acgagcagct ctccgttgcc gagatcacca actcgtgctt
     3781 cgagcccgcc aaccagatgg tcaagtgcga cccccgccac ggcaagtaca tggcctgctg
     3841 cgtcctctac cgcggtgacg tcgtccccaa ggacgtcaac gcctcgattg ccaccatcaa
     3901 gaccaagcgc accatccagt ttgtcgactg gtgccccact ggcttcaagg ttggcatcaa
     3961 ctaccagcca cccaccgtcg tgcccggcgg cgaccttgcc aaggtgcagc gctccgtctg
     4021 catgctcagc aacaccaccg ccattgccga ggcctgggct cgtcttgacc acaagttcga
     4081 cctcatgtac gccaagcgtg ccttcgtcca ctggtacgtc ggtgagggta tggaggaagg
     4141 cgagttcgcc gaggcccgcg aggatcttgc cgccctcgag aaggattacg aggaggtcgg
     4201 cgtcgactcc atggacggcg agggcgagga cgagggcgag gagtactaag cgcaaggccc
     4261 cctcccggcc tgacctccac caccaccgtc ttcactgctg caattacctg ccattgtttt
     4321 ggctgtcctt tgatgactgg ttgatgcttc tgcatttgtt ttgtgtgtgt tgagctgtct
     4381 ggtggtggcg cgtttgttca ctcttccacc ccttgccttt gctgcaagcg accgttccat
     4441 gtccccaacc atccaataca aacactgaat ccaacaacaa ttgcagcaaa gaggaataag
     4501 ttgtccaaca gaacgaagtt atgatgagtg ttgtgtggga tgtcacacat gacgcgacac
     4561 tggcagtggc tgttttgttt ggttggtggt ttactggtcc gctgcgtggc ggtagcgatc
     4621 atagaaggca agcatgtcta gaggatcccc gggtaccgag ctcgaattca ctggccgtcg
     4681 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     4741 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     4801 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
     4861 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     4921 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     4981 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     5041 caccgtcatc accgaaacgc g
//
