LOCUS       sequence-210242-       10498 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10498)
  AUTHORS   Ruppert S, Wang EH, Tjian R
  TITLE     Cloning and expression of human TAFII250: a TBP-associated factor 
            implicated in cell-cycle regulation.
  JOURNAL   Nature. 1993 Mar 11. 362(6416):175-9.
  PUBMED    7680771
REFERENCE   2  (bases 1 to 10498)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            1993 Mar 11. 362(6416):175-9."
COMMENT     Sequence Label: CMV-HA-hTAF1
FEATURES             Location/Qualifiers
     source          1..10498
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        7..984
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
     promoter        1019..1037
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1019..1036
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             1075..1101
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             1075..1101
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1102..1113
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(2690..2704)
                     /codon_start=1
                     /product="tag for protein cleavage using Ni2+ ion (Dang et 
                     al., 2019)"
                     /label=SNAC tag
                     /translation="GSHHW"
     polyA_signal    7534..7668
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7558..7577)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7612..7631
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7781..7801)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7781..7799)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7820..7836)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7820..7836)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7833..7855)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7844..7860
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7868..7874,7875..7892,7893..7898))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7913..7934
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8051..8068)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8222..8810)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8302..8321)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8981..9772,9773..9841))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9604..9623
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9842..9946)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9972..10427)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10109..10130)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10321..10340
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tcgaccgcca attcaatatg gcgtatatgg actcatgcca attcaatatg gtggatctgg
       61 acctgtgcca attcaatatg gcgtatatgg actcgtgcca attcaatatg gtggatctgg
      121 accccagcca attcaatatg gcggacttgg caccatgcca attcaatatg gcggacctgg
      181 cactgtgcca actggggagg ggtctacttg gcacggtgcc aagtttgagg aggggtcttg
      241 gccctgtgcc aagtccgcca tattgaattg gcatggtgcc aataatggcg gccatattgg
      301 ctatatgcca ggatcaatat ataggcaata tccaatatgg ccctatgcca atatggctat
      361 tggccaggtt caatactatg tattggccct atgccatata gtattccata tatgggtttt
      421 cctattgacg tagatagccc ctcccaatgg gcggtcccat ataccatata tggggcttcc
      481 taataccgcc catagccact cccccattga cgtcaatggt ctctatatat ggtctttcct
      541 attgacgtca tatgggcggt cctattgacg tatatggcgc ctcccccatt gacgtcaatt
      601 acggtaaatg gcccgcctgg ctcaatgccc attgacgtca ataggaccac ccaccattga
      661 cgtcaatggg atggctcatt gcccattcat atccgttctc acgcccccta ttgacgtcaa
      721 tgacggtaaa tggcccactt ggcagtacat caatatctat taatagtaac ttggcaagta
      781 cattactatt ggaagtacgc cagggtacat tggcagtact cccattgacg tcaatggcgg
      841 taaatggccc gcgatggctg ccaagtacat ccccattgac gtcaatgggg aggggcaatg
      901 acgcaaatgg gcgttccatt gacgtaaatg ggcggtaggc gtgcctaatg ggaggtctat
      961 ataagcaatg ctcgtttagg gaaccgccat tctgcctggg gacgtcggag caagcttgat
     1021 ttaggtgaca ctatagaata caagctactt gttctttttg caggatccat gggctacccc
     1081 tacgacgtgc ccgactacgc catcgaaggc cgccatatgg gacccggctg cgatttgctg
     1141 ctgcggacag cagctaccat cactgctgcc gccatcatgt cagacacgga cagcgacgaa
     1201 gattccgctg gaggcggccc attttcttta gcgggtttcc ttttcggcaa catcaatgga
     1261 gccgggcagc tggaggggga aagcgtcttg gatgatgaat gtaagaagca cttggcaggc
     1321 ttgggggctt tggggctggg cagcctgatc actgaactca cggcaaatga agaattgacc
     1381 gggactgacg gtgccttggt aaatgatgaa gggtgggtta ggagtacaga agatgctgtg
     1441 gactattcag acatcaatga ggtggcagaa gatgaaagcc gaagatacca gcagacgatg
     1501 gggagcttgc agcccctttg ccactcagat tatgatgaag atgactatga tgctgattgt
     1561 gaagacattg attgcaagtt gatgcctcct ccacctccac ccccgggacc aatgaagaag
     1621 gataaggacc aggattctat tactggtgtg tctgaaaatg gagaaggcat catcttgccc
     1681 tccatcattg ccccttcctc tttggcctca gagaaagtgg acttcagtag ttcctctgac
     1741 tcagaatctg agatgggacc tcaggaagca acacaggcag aatctgaaga tggaaagctg
     1801 acccttccat tggctgggat tatgcagcat gatgccacca agctgttgcc aagtgtcaca
     1861 gaactttttc cagaatttcg acctggaaag gtgttacgtt ttctacgtct ttttggacca
     1921 gggaagaatg tcccatctgt ttggcggagt gctcggagaa agaggaagaa gaagcaccgt
     1981 gagctgatac aggaagagca gatccaggag gtggagtgct cagtagaatc agaagtcagc
     2041 cagaagtctt tgtggaacta cgactacgct ccaccaccac ctccagagca gtgtctctct
     2101 gatgatgaaa tcacgatgat ggctcctgtg gagtccaaat tttcccaatc aactggagat
     2161 atagataaag tgacagatac caaaccaaga gtggctgagt ggcgttatgg gcctgcccga
     2221 ctgtggtatg atatgctggg tgtccctgaa gatggcagtg ggtttgacta tggcttcaaa
     2281 ctgagaaaga cagaacatga acctgtgata aaatctagaa tgatagagga atttaggaaa
     2341 cttgaggaaa acaatggcac tgatcttctg gctgatgaaa acttcctgat ggtgacacag
     2401 ctgcattggg aggatgatat catctgggat ggggaggatg tcaaacacaa agggacaaaa
     2461 cctcagcgtg caagcctggc aggctggctt ccttctagca tgactaggaa tgcgatggct
     2521 tacaatgttc agcaaggttt tgcagccact cttgatgatg acaaaccttg gtactccatt
     2581 tttcccattg acaatgagga tctggtatat ggacgctggg aggacaatat catttgggat
     2641 gctcaggcca tgccccggct gttggaacct cctgttttga cacttgatcc caatgatgag
     2701 aacctcattt tggaaattcc tgatgagaag gaagaggcca cctctaactc cccctccaag
     2761 gagagtaaga aggaatcatc tctgaagaag agtcgaattc tcttagggaa aacaggagtc
     2821 atcaaggagg aaccacagca gaacatgtct cagccagaag tgaaagatcc atggaatctc
     2881 tccaatgatg agtattatta tcccaagcaa cagggtcttc gaggcacctt tggagggaat
     2941 attatccagc attcaattcc tgctgtggaa ttacggcagc ccttctttcc cacccacatg
     3001 gggcccatca aactccggca gttccatcgc ccacctctga aaaagtactc atttggtgca
     3061 ctttctcagc caggtcccca ctcagtccaa cctttgctaa agcacatcaa aaaaaaggcc
     3121 aagatgagag aacaagagag gcaagcttca ggtggtggag agatgttttt tatgcgcaca
     3181 cctcaggacc tcacaggcaa agatggtgat cttattcttg cagaatatag tgaggaaaat
     3241 ggacccttaa tgatgcaggt tggcatggca accaagataa agaactatta taaacggaaa
     3301 cctggaaaag atcctggagc accagattgt aaatatgggg aaactgttta ctgccataca
     3361 tctcctttcc tgggttctct ccatcctggc caattgctgc aagcatttga gaacaacctt
     3421 tttcgtgctc caatttatct tcataagatg ccagaaactg atttcttgat cattcggaca
     3481 agacagggtt actatattcg ggaattagtg gatatttttg tggttggcca gcagtgtccc
     3541 ttgtttgaag ttcctgggcc taactccaaa agggccaata cgcatattcg agactttcta
     3601 caggttttta tttaccgcct tttctggaaa agtaaagatc ggccacggag gatacgaatg
     3661 gaagatataa aaaaagcctt tccttcccat tcagaaagca gcatccggaa gaggctaaag
     3721 ctctgcgctg acttcaaacg cacagggatg gactcaaact ggtgggtgct taagtctgat
     3781 tttcgtttac caacggaaga agagatcaga gctatggtgt caccagagca gtgctgtgct
     3841 tattatagca tgatagctgc agagcaacga ctgaaggatg ctggctatgg tgagaaatcc
     3901 ttttttgctc cagaagaaga aaatgaggaa gatttccaga tgaagattga tgatgaagtt
     3961 cgcactgccc cttggaacac cacaagggcc ttcattgctg ccatgaaggg caagtgtctg
     4021 ctagaggtga ctggggtggc agatcccacg gggtgtggtg aaggattctc ctatgtgaag
     4081 attccaaaca aaccaacaca gcagaaggat gataaagaac cgcagccagt gaagaagaca
     4141 gtgacaggaa cagatgcaga ccttcgtcgc ctttccctga aaaatgccaa gcaacttcta
     4201 cgtaaatttg gtgtgcctga ggaagagatt aaaaagttgt cccgctggga agtgattgat
     4261 gtggtgcgca caatgtcaac agaacaggct cgttctggag aggggcccat gagtaaattt
     4321 gcccgtggat caaggttttc tgtggctgag catcaagagc gttacaaaga ggaatgtcag
     4381 cgcatctttg acctacagaa caaggttctg tcatcaactg aagtcttatc aactgacaca
     4441 gacagcagct cagctgaaga tagtgacttt gaagaaatgg gaaagaacat tgagaacatg
     4501 ttgcagaaca agaaaaccag ctctcagctt tcacgtgaac gggaggaaca ggagcggaag
     4561 gaactacagc gaatgctact ggcagcaggc tcagcagcat ccggaaacaa tcacagagat
     4621 gatgacacag cttccgtgac tagccttaac tcttctgcca ctggacgctg tctcaagatt
     4681 tatcgcacgt ttcgagatga agaggggaaa gagtatgttc gctgtgagac agtccgaaaa
     4741 ccagctgtca ttgatgccta tgtgcgcata cggactacaa aagatgagga attcattcga
     4801 aaatttgccc tttttgatga acaacatcgg gaagagatgc gaaaagaacg gcggaggatt
     4861 caagagcaac tgaggcggct taagaggaac caggaaaagg agaagcttaa gggtcctcct
     4921 gagaagaagc ccaagaaaat gaaggagcgt cctgacctaa aactgaaatg tggggcatgt
     4981 ggtgccattg gacacatgag gactaacaaa ttctgccccc tctattatca aacaaatgcg
     5041 ccaccttcca accctgttgc catgacagaa gaacaggagg aggagttgga aaagacagtc
     5101 attcataatg ataatgaaga acttatcaag gttgaaggga ccaaaattgt cttggggaaa
     5161 cagctaattg agagtgcgga tgaggttcgc agaaaatctc tggttctcaa gtttcctaaa
     5221 cagcagcttc ctccaaagaa gaaacggcga gttggaacca ctgttcactg tgactatttg
     5281 aatagacctc ataagtccat ccaccggcgc cgcacagacc ctatggtgac gctgtcgtcc
     5341 atcttggagt ctatcatcaa tgacatgaga gatcttccaa atacataccc tttccacact
     5401 ccagtcaatg caaaggttgt aaaggactac tacaaaatca tcactcggcc aatggaccta
     5461 caaacactcc gcgaaaacgt gcgtaaacgc ctctacccat ctcgggaaga gttcagagag
     5521 catctggagc taattgtgaa aaatagtgca acctacaatg ggccaaaaca ctcattgact
     5581 cagatctctc aatccatgct ggatctctgt gatgaaaaac tcaaagagaa agaagacaaa
     5641 ttagctcgct tagagaaagc tatcaacccc ttgctggatg atgatgacca agtggcgttt
     5701 tctttcattc tggacaacat tgtcacccag aaaatgatgg cagttccaga ttcttggcca
     5761 tttcatcacc cagttaataa gaaatttgtt ccagattatt acaaagtgat tgtcaatcca
     5821 atggatttag agaccatacg taagaacatc tccaagcaca agtatcagag tcgggagagc
     5881 tttctggatg atgtaaacct tattctggcc aacagtgtta agtataatgg acctgagagt
     5941 cagtatacta agactgccca ggagattgtg aacgtctgtt accagacatt gactgagtat
     6001 gatgaacatt tgactcaact tgagaaggat atttgtactg ctaaagaagc agctttggag
     6061 gaagcagaat tagaaagcct ggacccaatg accccagggc cctacacgcc tcagcctcct
     6121 gatttgtatg ataccaacac atccctcagt atgtctcgag atgcctctgt atttcaagat
     6181 gagagcaata tgtctgtctt ggatattccc agtgccactc cagaaaagca ggtaacacag
     6241 gaaggtgaag atggagatgg tgatcttgca gatgaagagg aaggaactgt acaacagcct
     6301 caagccagtg tcctgtatga ggatttgctt atgtctgaag gagaagatga tgaggaagat
     6361 gctgggagtg atgaagaagg agacaatcct ttctctgcta tccagctgag tgaaagtgga
     6421 agtgactctg atgtgggatc tggtggaata agacccaaac aaccccgcat gcttcaggag
     6481 aacacaagga tggacatgga aaatgaagaa agcatgatgt cctatgaggg agacggtggg
     6541 gaggcttccc atggtttgga ggatagcaac atcagttatg ggagctatga ggagcctgat
     6601 cccaagtcga acacccaaga cacaagcttc agcagcatcg gtgggtatga ggtatcagag
     6661 gaggaagaag atgaggagga ggaagagcag cgctctgggc cgagcgtact aagccaggtc
     6721 cacctgtcag aggacgagga ggacagtgag gatttccact ccattgctgg ggacagtgac
     6781 ttggactctg atgaatgagg cttcctttgg gcctccttgg tcagccttcc ctgttctcca
     6841 gcctaggtgg ttcacctttc cccaatttgt tcatatttgt acagtatctg atcctgaaat
     6901 catgaaatta actaacacct tagccttttt aaaagtagta agtaaatgat aataaatcac
     6961 ctctcctaat cttcctgggg caatgtcacc ctttgattta aaacaaagca accccctttc
     7021 ccctaccact acggaaaaga gcaagctcat ttttccgtgt cctcctttat ttaactccat
     7081 ttattgcttt tggtataatt tttccctggg gaaggagggg aaattatgaa agaactagta
     7141 actttatgtc ctcttgatgt attaggaaat ttccggccag gcgtggtggc tcacacctgt
     7201 aatctcagca ctctgggagg ccgaggcggg cagatcacct gaggtcagaa gttcgagacc
     7261 agcttggcca acatggcgaa accgcatctc tactaaaaat acaaaaatta gccaggtgtg
     7321 gtggcgtatg cctgttaatc ctagctactc gggaggctga ggcaggagaa ttacttgaac
     7381 ccgggaggca gaggttgcag tgagtggagg tcacgccact gcactccagc ctgggcgaaa
     7441 gagtgagatt cagtctcaaa aaaaaaaaaa aaaaaaactc gagggggggc ccgcctctcg
     7501 agcctctaga actatagtga gtcgtattac gtagatccag acatgataag atacattgat
     7561 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     7621 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     7681 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta attcgcggcc
     7741 gcggcgccaa tgcattgggc ccggtaccca gcttttgttc cctttagtga gggttaattg
     7801 cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     7861 ttccacacaa catacgagcc gggagcataa agtgtaaagc ctggggtgcc taatgagtga
     7921 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     7981 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     8041 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     8101 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     8161 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     8221 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     8281 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     8341 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     8401 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     8461 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     8521 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     8581 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     8641 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     8701 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     8761 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     8821 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     8881 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     8941 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     9001 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     9061 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     9121 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     9181 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     9241 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     9301 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     9361 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     9421 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     9481 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     9541 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     9601 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     9661 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     9721 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     9781 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     9841 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     9901 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     9961 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
    10021 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
    10081 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
    10141 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
    10201 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
    10261 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
    10321 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
    10381 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt
    10441 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccag
//
