LOCUS       sequence-210244-        9219 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway compatible construct for N' terminal fusion or promoter 
            driven renilla luciferase reporter, hygromycin resistance cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9219)
  AUTHORS   Poliner E, Cummings C, Newton L, Farre EM
  TITLE     Identification of circadian rhythms in Nannochloropsis species using
            bioluminescence reporter lines.
  JOURNAL   Plant J. 2019 Jul;99(1):112-127. doi: 10.1111/tpj.14314. Epub 2019 
            Apr 19.
  PUBMED    30883973
REFERENCE   2  (bases 1 to 9219)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.14314";
            journalName: "Plant J"; date: "2019-07"; volume: "99"; issue: "1"; 
            pages: "112-127"
COMMENT     Sequence Label: pNOC-RLUC
FEATURES             Location/Qualifiers
     source          1..9219
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             801..1826
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             801..1826
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    2064..2188
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2213..2218,2219..2236,2237..2243)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2297..2956
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2363..2382)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3298..3603
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3482..3501
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3644..3768)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             4733..4759
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(6721..6738)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6892..7480)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6972..6991)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7651..8442,8443..8511))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8274..8293
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8512..8616)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8694..9149)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8831..8852)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9043..9062
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gtaggtctct aagatggagt ggatggagga ggaggcgagc gtagcagcaa gcgtgagtta
       61 tacagccagg cacatgtcgc aatccttcgg tctcgggctt aaaatccacg cactaatcac
      121 gctgggccat gcaaagagca atgccgaggc ccaccacaca aaacgctgtg tcgcgcgttg
      181 cggcctgaag cttcatactt cttagtcgcc gccaaaaggg ctcgagagac gagacccgtt
      241 ggcatgaccg atgttgttcg acgcggtttg cttcgtcaca gtcgacgtga ttcaggaatc
      301 tggagcctgc agatcatttt tttcagcctg atatcgttct tttccactga gaaccatcag
      361 accacctttt cttccattgt gtgaaggagt aggagttgcc gtgctgcttt gtgggagaca
      421 tctgcgatgg tgaccagcct cccgtcgtct ggtcgacgtg acgagcctct tcactgttct
      481 tcgacggaga gacgcaagcg agacggctct agaccttttg gacacgcatt ctgtgtgtga
      541 actagtggac agtgatacca cgtctgaaag ctcaccactg cccatggtgc agctacttgt
      601 cacaaagttt tgactccgtc ggtatcacca ttcgcgctcg tgtgcctggt tgttccgcca
      661 cgccggcctg ccccggggcg gggcaatatt ctaaaatctc acgcaaaaca ccgcacttac
      721 ccctcacaca tattcgtgat agaccaccac caatctcagc ccgcatcaac acagtcgagg
      781 gccccggggg gcaataagat atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt
      841 ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat
      901 ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg
      961 ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga
     1021 ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc
     1081 gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc
     1141 cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg
     1201 gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga
     1261 ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg
     1321 tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc
     1381 tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg
     1441 tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct
     1501 tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc
     1561 cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac
     1621 tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg
     1681 acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg
     1741 cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca
     1801 gcactcgtcc gagggcaaag gaatagagta gatgccgacc ggagtccgca aaaatcacca
     1861 gtctctctct acaaatctat ctctctctat ttttctccag aataatgtgt gagtagttcc
     1921 cagataaggg aattagggtt cttatagggt ttcgctcatg tgttgagcat ataagaaacc
     1981 cttagtatgt atttgtattt gtaaaatact tctatcaata aaatttctaa ttcctaaaac
     2041 caaaatccag tgacccgata tcaacaagtt tgtacaaaaa agctgaacga gaaacgtaaa
     2101 atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact
     2161 gtaaaacaca acatatccag tcactatggc ggccgcatta ggcaccccag gctttacact
     2221 ttatgcttcc ggctcgtata atgtgtggat tttgagttag gatccgtcga gattttcagg
     2281 agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca
     2341 atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca
     2401 gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt
     2461 ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat
     2521 ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt
     2581 ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca
     2641 gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc
     2701 taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag
     2761 ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa
     2821 atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt
     2881 ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg
     2941 gcagggcggg gcgtaaagat ctggatccgg cttactaaaa gccagataac agtatgcgta
     3001 tttgcgcgct gatttttgcg gtataagaat atatactgat atgtataccc gaagtatgtc
     3061 aaaaagaggt atgctatgaa gcagcgtatt acagtgacag ttgacagcga cagctatcag
     3121 ttgctcaagg catatatgat gtcaatatct ccggtctggt aagcacaacc atgcagaatg
     3181 aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg atggctgagg
     3241 tcgcccggtt tattgaaatg aacggctctt ttgctgacga gaacaggggc tggtgaaatg
     3301 cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt ggatgtacag
     3361 agtgatatta ttgacacgcc cgggcgacgg atggtgatcc ccctggccag tgcacgtctg
     3421 ctgtcagata aagtctcccg tgaactttac ccggtggtgc atatcgggga tgaaagctgg
     3481 cgcatgatga ccaccgatat ggccagtgtg ccggtctccg ttatcgggga agaagtggct
     3541 gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt ctggggaata
     3601 taaatgtcag gctcccttat acacagccag tctgcaggtc gaccatagtg actggatatg
     3661 ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat
     3721 atttatatca ttttacgttt ctcgttcagc tttcttgtac aaagtggttg atagcttggc
     3781 gcgcctcatg ggcggatccg gcgccagcaa ggtgtacgac cccgagcagc gcaagcgcat
     3841 gatcaccggc cctcagtggt gggctcgctg caagcagatg aacgtgctgg acagcttcat
     3901 caactactac gacagcgaga agcacgccga gaacgccgtg atcttcctgc acggcaacgc
     3961 cgccagcagc tacctgtggc gccacgtggt gccccacatc gagcccgtgg cccgctgcat
     4021 catccccgac ctgatcggca tgggcaagag cggcaagagc ggcaacggca gctaccgcct
     4081 gctggaccac tacaagtacc tgaccgcctg gttcgagctg ctgaacctgc ccaagaagat
     4141 catcttcgtg ggccacgact ggggcgcctg cctggccttc cactacagct acgagcacca
     4201 ggacaagatc aaggccatcg tgcacgccga gagcgtggtg gacgtgatcg agagctggga
     4261 cgagtggccc gacatcgagg aggacatcgc cctgatcaag agcgaggagg gcgagaagat
     4321 ggtgctggag aacaacttct tcgtggagac catgctgccc agcaagatca tgcgcaagct
     4381 ggagcccgag gagttcgccg cctacctgga gcccttcaag gagaagggcg aggtgcgccg
     4441 tcccaccctg agctggcctc gcgagatccc cctggtgaag ggcggcaagc ccgacgtggt
     4501 gcagatcgtg cgcaactaca acgcctactt gcgcgccagc gacgacctgc ccaagatgtt
     4561 catcgagagc gaccccggct tcttcagcaa cgccatcgtg gagggcgcca agaagttccc
     4621 caacaccgag ttcgtgaagg tgaagggcct gcacttcagc caggaggacg ctcccgacga
     4681 gatgggcaag tacatcaaga gcttcgtgga gcgcgtgctg aagaacgata cgtaccccta
     4741 tgacgtgccg gactacgcct gagctcgaaa gatccaagag agacgagtag agattttttt
     4801 tttgggattg atgtttgtcg ttctttgagt tgtcgtcgag ttacgccttt tgtaagaatg
     4861 ttccgcagga gaggaggagg atgggcatga gtgagggtga gagggcttgc ccgctttttt
     4921 ttttaaaaac gctgaagacg tggttgtcaa acaaaccccc catagaaacg attttgttac
     4981 ggtgcggtcc agacgtcact tgaatggctc cgcggaaagg ccagggaggg aaggggggag
     5041 ggaggaaaca tgaaacatgt tgaacggctc aacagggttt gggggacaag agaggtagcg
     5101 ccctgatgga ctgctccctc ccctcctttc cctcaatgtc tcattcatcc atgcttcccc
     5161 cttctctctc tcccctccgt tccatccccc gcgggcgtgg tagtggcgtg atgggatcca
     5221 ctaaaatgta cgtgtaagaa aagccggtga gcttacgctt ttgtgaaagt gggagtacga
     5281 gtgttgtgtg tgtgtgtagt ggtttcagac cccagacaga ggcgaagcag aaaaagcaga
     5341 cgatgaagac gacgaagaaa tgagcagtct atttttatcg tggaaacaga agaggtgata
     5401 tcgtctcgtt ctttgttatc acctaccccg cgtgcatgta catgcagcct ttttattttg
     5461 taatctttcc cgaaaaatca accgccacct cccccccgcc ttctctcacc catcatcttc
     5521 tcctgtttat cttctacttt acactagatc gcatggcaca tctccctcgc aatccatcgg
     5581 tgcaaccatc atcgatccca ctcctccctc cctccctccc tccctcctcg catctcttct
     5641 aagaaatccg ctagctgcga acgcagctca cctacctcat ttatcagagc ctcgattcgg
     5701 tccttttcct tctcccctac ctcgccgcaa acagcaacag aagcaggagc agcaggagca
     5761 gcggcacctg tgtcaacagc acaagggcaa gtacagacag aagacgaggc ggaggaggag
     5821 gaagacgatg tgatgtcatt ggagctggct aaactattag ccgttgctct caccgccttt
     5881 ccccaaaact cttccaccca tcgtgtcact ccttctatca ggggaaccat ccctcttttc
     5941 ctccccacct tcttcccctt gctcttcttc ttcctttgtc tctttgttcc ccctcctctc
     6001 ttcgcctcct cctccgcatc gtcctctcct tttatcaagt cttgcgttgc aaaggaagca
     6061 agctctttgt gaaggaccgc gcaagggata ggagagtgga gagtgaggtg atgcagacaa
     6121 accgaggatt cccgggcgtc agataaccta aaatcgacta cttcttgagg tggaggagga
     6181 gggggaggag acggagaata agtgtgtgta gaaatatagc ttgctgcctc tgcttccatc
     6241 ttcgataaaa ttgctgttgc tgttgctgtt gcggctgata tctgctgcgg ctgctgctgc
     6301 acctcctgaa cgaagactgc agccgcgtct tcaccctctt ggactactct gccctcctcc
     6361 cttgccttta caactcgatc gacgagattc gtgccagtgc tgtcatcatc attggaaaga
     6421 gcaacagcat caccttaaga ttgaatcctg ttgccggtct tgcgatgatt atcatataat
     6481 ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga
     6541 gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa
     6601 tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctaggctgca
     6661 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     6721 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     6781 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     6841 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     6901 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     6961 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     7021 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     7081 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     7141 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     7201 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     7261 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     7321 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     7381 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     7441 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     7501 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     7561 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     7621 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     7681 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     7741 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     7801 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     7861 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     7921 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     7981 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     8041 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     8101 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     8161 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     8221 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     8281 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     8341 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     8401 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     8461 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     8521 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     8581 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     8641 tgatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggaaattgt
     8701 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
     8761 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
     8821 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     8881 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
     8941 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     9001 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     9061 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     9121 cgcgcttaat gcgccgctac agggcgcgtc cattcgccat tcaggctgcg caactgttgg
     9181 gaagggcgat cggtgcgggc ctcttcgcta ttacgccag
//
