LOCUS       sequence-210250-        6370 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6370)
  AUTHORS   Beraud C, Sun SC, Ganchi P, Ballard DW, Greene WC
  TITLE     Human T-cell leukemia virus type I Tax associates with and is 
            negatively regulated by the NF-kappa B2 p100 gene product: 
            implications for viral latency.
  JOURNAL   Mol Cell Biol. 1994 Feb . 14(2):1374-82.
  PUBMED    8289813
REFERENCE   2  (bases 1 to 6370)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 1994 Feb . 14(2):1374-82."
COMMENT     Sequence Label: pCMV4-p52
FEATURES             Location/Qualifiers
     source          1..6370
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        42..421
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        422..625
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     576..596
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(2238..2254)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2238..2254)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     polyA_signal    2262..2884
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2461..2480)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(2908..2928)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2913..3242
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3093..3228
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          3281..3377
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     primer_bind     complement(3453..3472)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3454..3472)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3486..3502)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3486..3502)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3499..3521)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3510..3526
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3534..3540,3541..3558,3559..3564))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3579..3600
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3717..3734)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3888..4476)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3968..3987)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4647..5438,5439..5507))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5270..5289
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5508..5612)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5639..6094
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5726..5745)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5936..5957
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6220..6242
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6234..6251
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6235..6251
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtacatttat attggctcat gtccaatatg accgccatgt tgacattgat tattgactag
       61 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
      121 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      181 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      241 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      301 tccgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      361 gaccttacgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      421 ggtgatgcgg ttttggcagt acaccaatgg gcgtggatag cggtttgact cacggggatt
      481 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
      541 ctttccaaaa tgtcgtaata accccgcccc gttgacgcaa atgggcggta ggcgtgtacg
      601 gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagaattgtt tttattttta
      661 attttctttc aaatacttcc atcgaattca gatctggtac cacgcgtatc gataagcttc
      721 accatggaga gttgctacaa cccaggtctg gatggtatta ttgaatatga tgatttcaaa
      781 ttgaactcct ccattgtgga acccaaggag ccagccccag aaacagctga tggcccctac
      841 ctggtgatcg tggaacagcc taagcagaga ggcttccgat ttcgatatgg ctgtgaaggc
      901 ccctcccatg gaggactgcc cggtgcctcc agtgagaagg gccgaaagac ctatcccact
      961 gtcaagatct gtaactacga gggaccagcc aagatcgagg tggacctggt aacacacagt
     1021 gacccacctc gtgctcatgc ccacagtctg gtgggcaagc aatgctcgga gctggggatc
     1081 tgcgccgttt ctgtggggcc caaggacatg actgcccaat ttaacaacct gggtgtcctg
     1141 catgtgacta agaagaacat gatggggact atgatacaaa aacttcagag gcagcggctc
     1201 cgctctaggc cccagggcct tacggaggcc gagcagcggg agctggagca agaggccaaa
     1261 gaactgaaga aggtgatgga tctgagtata gtgcggctgc gcttctctgc cttccttaga
     1321 gccagtgatg gctccttctc cctgcccctg aagccagtca tctcccagcc catccatgac
     1381 agcaaatctc cgggggcatc aaacctgaag atttctcgaa tggacaagac agcaggctct
     1441 gtgcggggtg gagatgaagt ttatctgctt tgtgacaagg tgcagaaaga tgacattgag
     1501 gttcggttct atgaggatga tgagaatgga tggcaggcct ttggggactt ctctcccaca
     1561 gatgtgcata aacagtatgc cattgtgttc cggacacccc cctatcacaa gatgaagatt
     1621 gagcggcctg taacagtgtt tctgcaactg aaacgcaagc gaggagggga cgtgtctgat
     1681 tccaaacagt tcacctatta ccctctggtg gaagacaagg aagaggtgca gcggaagcgg
     1741 aggaaggcct tgcccacctt ctcccagccc ttcgggggtg gctcccacat gggtggaggc
     1801 tctgggggtg cagccggggg ctacggagga gctggaggag gtgagggggt actgatggag
     1861 ggaggggtaa aggtaagaga agctgtggag gaaaaaaatc tgggggaggc cgggcgtggt
     1921 ggctcacgcc tgtaatccag ccctttggga ggccaaggca ggcagattac ctgagatcag
     1981 gagttcaaga ccagcttggc caacagcgtg aaacctcgtc tctactaaaa atacaaacat
     2041 tagctgggca tggtggcagg cgcctgtaat cccagctact cgggaggctg aggcaggaga
     2101 atcgcttgaa ccctgggaga caagaggttg cagtaagctg agatcacacc actgcactcc
     2161 aggctgggca ataagagcga aactccgtct caaaaaaaaa aaaaaaaaaa gcggccgcga
     2221 attcctgcag cccgggggat ccactagttc tagaggatcc cgggtggcat ccctgtgacc
     2281 cctccccagt gcctctcctg gccctggaag ttgccactcc agtgcccacc agccttgtcc
     2341 taataaaatt aagttgcatc attttgtctg actaggtgtc cttctataat attatggggt
     2401 ggaggggggt ggtatggagc aaggggcaag ttgggaagac aacctgtagg gcctgcgggg
     2461 tctattggga accaagctgg agtgcagtgg cacaatcttg gctcactgca atctccgcct
     2521 cctgggttca agcgattctc ctgcctcagc ctcccgagtt gttgggattc caggcatgca
     2581 tgaccaggct cagctaattt ttgttttttt ggtagagacg gggtttcacc atattggcca
     2641 ggctggtctc caactcctaa tctcaggtga tctacccacc ttggcctccc aaattgctgg
     2701 gattacaggc gtgaaccact gctcccttcc ctgtccttct gattttaaaa taactatacc
     2761 agcaggagga cgtccagaca cagcataggc tacctggcca tgcccaaccg gtgggacatt
     2821 tgagttgctt gcttggcact gtcctctcat gcgttgggtc cactcagtag atgcctgttg
     2881 aattgggtac gcggccagct tggctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     2941 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     3001 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     3061 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     3121 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     3181 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     3241 aaaagctcct cgaggaactg aaaaaccaga aagttaactg gtaagtttag tctttttgtc
     3301 ttttatttca ggtcccggat ccggtggtgg tgcaaatcaa agaactgctc ctcagtggat
     3361 gttgccttta cttctaggcc tgtacggaag tgttacttct gctctaaaag ctgctgcagg
     3421 tcgactctag ggccgcgtac cgagctcgaa ttccctatag tgagtcgtat taaattcgta
     3481 atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     3541 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     3601 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     3661 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     3721 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     3781 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     3841 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     3901 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     3961 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     4021 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     4081 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     4141 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     4201 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     4261 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     4321 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     4381 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     4441 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     4501 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     4561 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     4621 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     4681 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     4741 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     4801 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     4861 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     4921 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     4981 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     5041 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     5101 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     5161 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     5221 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     5281 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     5341 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     5401 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     5461 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     5521 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     5581 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     5641 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     5701 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     5761 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     5821 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     5881 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     5941 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     6001 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     6061 gcgaatttta acaaaatatt aacgcttaca atttccattc gccattcagg ctgcgcaact
     6121 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
     6181 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
     6241 cgacggccag tgccaagctg atctatacat tgaatcaata ttggcaatta gccatattag
     6301 tcattggtta tatagcataa atcaatattg gctattggcc attgcatacg ttgtatctat
     6361 atcataatat
//
