LOCUS       sequence-210257-        9944 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway compatible construct for N' terminal fusion or promoter 
            driven firefly luciferase reporter, hygromycin resistance cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9944)
  AUTHORS   Poliner E, Cummings C, Newton L, Farre EM
  TITLE     Identification of circadian rhythms in Nannochloropsis species using
            bioluminescence reporter lines.
  JOURNAL   Plant J. 2019 Jul;99(1):112-127. doi: 10.1111/tpj.14314. Epub 2019 
            Apr 19.
  PUBMED    30883973
REFERENCE   2  (bases 1 to 9944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.14314";
            journalName: "Plant J"; date: "2019-07"; volume: "99"; issue: "1"; 
            pages: "112-127"
COMMENT     Sequence Label: pNOC-FLUC
FEATURES             Location/Qualifiers
     source          1..9944
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             801..1826
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             801..1826
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    2064..2188
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2213..2218,2219..2236,2237..2243)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2297..2956
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2363..2382)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3298..3603
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3482..3501
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3644..3768)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             5456..5482
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(7446..7463)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7617..8205)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7697..7716)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8376..9167,9168..9236))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8999..9018
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9237..9341)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9419..9874)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9556..9577)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9768..9787
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gtaggtctct aagatggagt ggatggagga ggaggcgagc gtagcagcaa gcgtgagtta
       61 tacagccagg cacatgtcgc aatccttcgg tctcgggctt aaaatccacg cactaatcac
      121 gctgggccat gcaaagagca atgccgaggc ccaccacaca aaacgctgtg tcgcgcgttg
      181 cggcctgaag cttcatactt cttagtcgcc gccaaaaggg ctcgagagac gagacccgtt
      241 ggcatgaccg atgttgttcg acgcggtttg cttcgtcaca gtcgacgtga ttcaggaatc
      301 tggagcctgc agatcatttt tttcagcctg atatcgttct tttccactga gaaccatcag
      361 accacctttt cttccattgt gtgaaggagt aggagttgcc gtgctgcttt gtgggagaca
      421 tctgcgatgg tgaccagcct cccgtcgtct ggtcgacgtg acgagcctct tcactgttct
      481 tcgacggaga gacgcaagcg agacggctct agaccttttg gacacgcatt ctgtgtgtga
      541 actagtggac agtgatacca cgtctgaaag ctcaccactg cccatggtgc agctacttgt
      601 cacaaagttt tgactccgtc ggtatcacca ttcgcgctcg tgtgcctggt tgttccgcca
      661 cgccggcctg ccccggggcg gggcaatatt ctaaaatctc acgcaaaaca ccgcacttac
      721 ccctcacaca tattcgtgat agaccaccac caatctcagc ccgcatcaac acagtcgagg
      781 gccccggggg gcaataagat atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt
      841 ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat
      901 ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg
      961 ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga
     1021 ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc
     1081 gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc
     1141 cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg
     1201 gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga
     1261 ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg
     1321 tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc
     1381 tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg
     1441 tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct
     1501 tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc
     1561 cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac
     1621 tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg
     1681 acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg
     1741 cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca
     1801 gcactcgtcc gagggcaaag gaatagagta gatgccgacc ggagtccgca aaaatcacca
     1861 gtctctctct acaaatctat ctctctctat ttttctccag aataatgtgt gagtagttcc
     1921 cagataaggg aattagggtt cttatagggt ttcgctcatg tgttgagcat ataagaaacc
     1981 cttagtatgt atttgtattt gtaaaatact tctatcaata aaatttctaa ttcctaaaac
     2041 caaaatccag tgacccgata tcaacaagtt tgtacaaaaa agctgaacga gaaacgtaaa
     2101 atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact
     2161 gtaaaacaca acatatccag tcactatggc ggccgcatta ggcaccccag gctttacact
     2221 ttatgcttcc ggctcgtata atgtgtggat tttgagttag gatccgtcga gattttcagg
     2281 agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca
     2341 atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca
     2401 gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt
     2461 ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat
     2521 ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt
     2581 ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca
     2641 gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc
     2701 taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag
     2761 ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa
     2821 atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt
     2881 ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg
     2941 gcagggcggg gcgtaaagat ctggatccgg cttactaaaa gccagataac agtatgcgta
     3001 tttgcgcgct gatttttgcg gtataagaat atatactgat atgtataccc gaagtatgtc
     3061 aaaaagaggt atgctatgaa gcagcgtatt acagtgacag ttgacagcga cagctatcag
     3121 ttgctcaagg catatatgat gtcaatatct ccggtctggt aagcacaacc atgcagaatg
     3181 aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg atggctgagg
     3241 tcgcccggtt tattgaaatg aacggctctt ttgctgacga gaacaggggc tggtgaaatg
     3301 cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt ggatgtacag
     3361 agtgatatta ttgacacgcc cgggcgacgg atggtgatcc ccctggccag tgcacgtctg
     3421 ctgtcagata aagtctcccg tgaactttac ccggtggtgc atatcgggga tgaaagctgg
     3481 cgcatgatga ccaccgatat ggccagtgtg ccggtctccg ttatcgggga agaagtggct
     3541 gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt ctggggaata
     3601 taaatgtcag gctcccttat acacagccag tctgcaggtc gaccatagtg actggatatg
     3661 ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat
     3721 atttatatca ttttacgttt ctcgttcagc tttcttgtac aaagtggttg atagcttggc
     3781 gcgcctcatg ggcgggtccg gcgaggacgc aaagaatatc aagaaaggcc ctgcaccgtt
     3841 ctaccctttg gaggacggga ccgcgggaga gcagcttcac aaggctatga aacgttacgc
     3901 tcttgttccg ggcacgatcg cattcacgga tgcccacatc gaggtcgaca ttacatatgc
     3961 tgagtacttc gagatgtccg tgcgccttgc cgaagctatg aagcgttacg ggctgaacac
     4021 gaaccaccgc atcgtggtct gttcagagaa ttccctacag ttctttatgc ccgtgttggg
     4081 ggcccttttc attggcgtcg ccgtggcccc cgccaacgac atttataacg agcgcgagct
     4141 gctcaacagc atggggattt cgcagccgac tgtggtcttc gtttccaaga aaggtttgca
     4201 aaagatattg aatgtacaga agaaactgcc cattatccag aagattatca ttatggactc
     4261 taagaccgat taccagggat tccagtcgat gtacactttc gtcacgagcc atctcccacc
     4321 cgggttcaac gagtacgact tcgtgccgga gagcttcgac cgcgacaaga caatcgccct
     4381 tattatgaac tctagtggta gcaccggttt gcccaagggc gtggcgctgc cccatcgtac
     4441 cgcctgcgtg cgcttctccc atgcgcgtga tcctatcttc ggcaaccaga tcattccgga
     4501 caccgcgatt ctttcggtcg tgccgtttca ccatggattt ggcatgttta cgactttggg
     4561 ctacctcatc tgcggcttcc gggtggtcct gatgtaccgg tttgaggaag agctgttttt
     4621 gcgcagtctg caggactaca agatccagtc cgcgctgctc gtccctacac tcttttcttt
     4681 ctttgctaag tccaccttaa tcgacaagta cgatctgtca aacctccacg agatcgcctc
     4741 cggaggtgcg cccttatcta aggaagtcgg ggaggctgtc gcgaagagat ttcacctgcc
     4801 cggcatcagg cagggatatg gcctgaccga gaccacttcc gccatcttga tcacgcccga
     4861 gggtgacgat aaaccaggcg ccgtggggaa agtggtccct tttttcgaag caaaggtcgt
     4921 tgacctggac accggcaaga cgctcggggt gaaccaacga ggtgagttgt gcgtgcgcgg
     4981 ccctatgatt atgagcgggt acgtgaacaa tcccgaagcc accaacgcac tcattgataa
     5041 ggacggatgg ctacacagcg gtgatatcgc ctactgggat gaggacgagc acttcttcat
     5101 cgtagaccgg ctgaagtcgc tcatcaagta caaggggtat caggtggccc ctgcggagtt
     5161 ggaatctatt ctgctccaac accctaatat cttcgacgcc ggcgtggccg gcttgcccga
     5221 cgatgacgcg ggcgagcttc ccgccgcggt tgtggtcctg gagcacggca agacgatgac
     5281 cgagaaggag atcgtagact acgtggcttc ccaagtgact acggcgaaga agttgagggg
     5341 tggagtagtg ttcgtggatg aggtccccaa ggggctcacc ggtaagctcg atgcccgtaa
     5401 gatccgagag atcttgatta aggctaagaa ggggggcaag atcgccgtgt cgtcgtatcc
     5461 ctacgatgtt cccgattatg cctaagagct cgaaagatcc aagagagacg agtagagatt
     5521 ttttttttgg gattgatgtt tgtcgttctt tgagttgtcg tcgagttacg ccttttgtaa
     5581 gaatgttccg caggagagga ggaggatggg catgagtgag ggtgagaggg cttgcccgct
     5641 ttttttttta aaaacgctga agacgtggtt gtcaaacaaa ccccccatag aaacgatttt
     5701 gttacggtgc ggtccagacg tcacttgaat ggctccgcgg aaaggccagg gagggaaggg
     5761 gggagggagg aaacatgaaa catgttgaac ggctcaacag ggtttggggg acaagagagg
     5821 tagcgccctg atggactgct ccctcccctc ctttccctca atgtctcatt catccatgct
     5881 tcccccttct ctctctcccc tccgttccat cccccgcggg cgtggtagtg gcgtgatggg
     5941 atccactaaa atgtacgtgt aagaaaagcc ggtgagctta cgcttttgtg aaagtgggag
     6001 tacgagtgtt gtgtgtgtgt gtagtggttt cagaccccag acagaggcga agcagaaaaa
     6061 gcagacgatg aagacgacga agaaatgagc agtctatttt tatcgtggaa acagaagagg
     6121 tgatatcgtc tcgttctttg ttatcaccta ccccgcgtgc atgtacatgc agccttttta
     6181 ttttgtaatc tttcccgaaa aatcaaccgc cacctccccc ccgccttctc tcacccatca
     6241 tcttctcctg tttatcttct actttacact agatcgcatg gcacatctcc ctcgcaatcc
     6301 atcggtgcaa ccatcatcga tcccactcct ccctccctcc ctccctccct cctcgcatct
     6361 cttctaagaa atccgctagc tgcgaacgca gctcacctac ctcatttatc agagcctcga
     6421 ttcggtcctt ttccttctcc cctacctcgc cgcaaacagc aacagaagca ggagcagcag
     6481 gagcagcggc acctgtgtca acagcacaag ggcaagtaca gacagaagac gaggcggagg
     6541 aggaggaaga cgatgtgatg tcattggagc tggctaaact attagccgtt gctctcaccg
     6601 cctttcccca aaactcttcc acccatcgtg tcactccttc tatcagggga accatccctc
     6661 ttttcctccc caccttcttc cccttgctct tcttcttcct ttgtctcttt gttccccctc
     6721 ctctcttcgc ctcctcctcc gcatcgtcct ctccttttat caagtcttgc gttgcaaagg
     6781 aagcaagctc tttgtgaagg accgcgcaag ggataggaga gtggagagtg aggtgatgca
     6841 gacaaaccga ggattcccgg gcgtcagata acctaaaatc gactacttct tgaggtggag
     6901 gaggaggggg aggagacgga gaataagtgt gtgtagaaat atagcttgct gcctctgctt
     6961 ccatcttcga taaaattgct gttgctgttg ctgttgcggc tgatatctgc tgcggctgct
     7021 gctgcacctc ctgaacgaag actgcagccg cgtcttcacc ctcttggact actctgccct
     7081 cctcccttgc ctttacaact cgatcgacga gattcgtgcc agtgctgtca tcatcattgg
     7141 aaagagcaac agcatcacct taagattgaa tcctgttgcc ggtcttgcga tgattatcat
     7201 ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt
     7261 tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa
     7321 caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactagg
     7381 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     7441 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     7501 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     7561 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     7621 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     7681 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     7741 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     7801 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     7861 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     7921 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     7981 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     8041 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     8101 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     8161 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     8221 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     8281 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     8341 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     8401 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     8461 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     8521 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     8581 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     8641 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     8701 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     8761 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     8821 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     8881 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     8941 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     9001 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     9061 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     9121 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     9181 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     9241 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     9301 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     9361 ccacctgatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggaa
     9421 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
     9481 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
     9541 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
     9601 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
     9661 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
     9721 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
     9781 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
     9841 cccgccgcgc ttaatgcgcc gctacagggc gcgtccattc gccattcagg ctgcgcaact
     9901 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccag
//
