LOCUS       sequence-210259-        4714 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of OPNb.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4714)
  TITLE     Georg Weber Lab Plasmids
REFERENCE   2  (bases 1 to 4714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Bluescript-OPNb
FEATURES             Location/Qualifiers
     source          1..4714
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    39..60
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(75..80,81..98,99..105)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    113..129
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     118..140
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     137..153
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     137..153
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        174..192
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     229..245
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     229..245
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2034..2050)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2035..2051)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(2075..2094)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2076..2094)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2101..2118)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2101..2117)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2110..2132)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(2259..2714)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2396..2417)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2608..2627
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2741..2845
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2846..2914,2915..3706)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3064..3083)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3877..4465
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4366..4385
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4619..4636
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
       61 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
      121 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct cgaaattaac
      181 cctcactaaa gggaacaaaa gctggagctc caccgcggtg gcggccgctc tagaactagt
      241 ggatcccccg ggctgcagga attccgtaga ggcagcagca gcaggaggag gcagagcaca
      301 gcatcgtcgg gaccagactc gtctcaggcc agttgcagcc ttctcagcca aacgccgacc
      361 aaggaaaact cactaccatg agaattgcag tgatttgctt ttgcctccta ggcatcacct
      421 gtgccatacc agttaaacag gctgattctg gaagttctga ggaaaagcag ctttacaaca
      481 aatacccaga tgctgtggcc acatggctaa accctgaccc atctcagaag cagaatctcc
      541 tagccccaca gacccttcca agtaagtcca acgaaagcca tgaccacatg gatgatatgg
      601 atgatgaaga tgatgatgac catgtggaca gccaggactc cattgactcg aacgactctg
      661 atgatgtaga tgacactgat gattctcacc agtctgatga gtctcaccat tctgatgaat
      721 ctgatgaact ggtcactgat tttcccacgg acctgccagc aaccgaagtt ttcactccag
      781 ttgtccccac agtagacaca tatgatggcc gaggtgatag tgtggtttat ggactgaggt
      841 caaaatctaa gaagtttcgc agacctgaca tccagtaccc tgatgctaca gacgaggaca
      901 tcacctcaca catggaaagc gaggagttga atggtgcata caaggccatc cccgttgccc
      961 aggacctgaa cgcgccttct gattgggaca gccgtgggaa ggacagttat gaaacgagtc
     1021 agctggatga ccagagtgct gaaacccaca gccacaagca gtccagatta tataagcgga
     1081 aagccaatga tgagagcaat gagcattccg atgtgattga tagtcaggaa ctttccaaag
     1141 tcagccgtga attccacagc catgaatttc acagccatga agatatgctg gttgtagacc
     1201 ccaaaagtaa ggaagaagat aaacacctga aatttcgtat ttctcatgaa ttagatagtg
     1261 catcttctga ggtcaattaa aaggagaaaa aatacaattt ctcactttgc atttagtcaa
     1321 aagaaaaaat gctttatagc aaaatgaaag agaacatgaa atgcttcttt ctcagtttat
     1381 tggttgaatg tgtatctatt tgagtctgga aataactaat gtgtttgata attagtttag
     1441 tttgtggctt catggaaact ccctgtaaac taaaagcttc agggttatgt ctatgttcat
     1501 tctatagaag aaatgcaaac tatcactgta ttttaatatt tgttattctc tcatgaatag
     1561 aaatttatgt agaagcaaac aaaatacttt tacccactta aaaagagaat ataacatttt
     1621 atgtcactat aatcttttgt tttttaagtt agtgtatatt ttgttgtgat tatctttttg
     1681 tggtgtgaat aaatctttta tcttgaatgt aataagaaaa aaaaaaaaaa aaaaaaaaaa
     1741 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     1801 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     1861 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     1921 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     1981 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaggaattcg atatcaagct tatcgatacc
     2041 gtcgacctcg agggggggcc cggtacccaa ttcgccctat agtgagtcgt attacaattc
     2101 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     2161 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     2221 cccttcccaa cagttgcgca gcctgaatgg cgaatggcaa attgtaagcg ttaatatttt
     2281 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
     2341 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
     2401 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
     2461 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
     2521 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
     2581 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
     2641 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
     2701 gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     2761 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     2821 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     2881 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     2941 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     3001 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     3061 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     3121 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     3181 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     3241 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     3301 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     3361 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     3421 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     3481 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     3541 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     3601 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     3661 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     3721 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     3781 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     3841 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     3901 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     3961 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     4021 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     4081 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     4141 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     4201 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     4261 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     4321 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     4381 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     4441 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     4501 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     4561 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     4621 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     4681 ttggccgatt cattaatgca gctggcacga cagg
//
