LOCUS       sequence-210260-       10995 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway compatible construct for N' terminal fusion or promoter 
            driven firefly luciferase reporter, zeocin resistance cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10995)
  AUTHORS   Poliner E, Cummings C, Newton L, Farre EM
  TITLE     Identification of circadian rhythms in Nannochloropsis species using
            bioluminescence reporter lines.
  JOURNAL   Plant J. 2019 Jul;99(1):112-127. doi: 10.1111/tpj.14314. Epub 2019 
            Apr 19.
  PUBMED    30883973
REFERENCE   2  (bases 1 to 10995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.14314";
            journalName: "Plant J"; date: "2019-07"; volume: "99"; issue: "1"; 
            pages: "112-127"
COMMENT     Sequence Label: pNSA-FLUC
FEATURES             Location/Qualifiers
     source          1..10995
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1753..2127
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      2862..2871
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    2921..3045
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3070..3075,3076..3093,3094..3100)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3154..3813
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3220..3239)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4155..4460
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4339..4358
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(4501..4625)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             6313..6339
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(8120..8138)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8121..8138)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8156..8172)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8156..8172)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8169..8191)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8180..8196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8204..8210,8211..8228,8229..8234))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8249..8270
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8387..8404)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8558..9146)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8638..8657)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9317..10108,10109..10177))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9940..9959
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10178..10282)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(10360..10815)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10497..10518)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10709..10728
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10941..10963
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10955..10972
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10956..10972
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(10979..10995,1..3)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(10979..10995,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat
       61 atcactagag tatagctaca tggtagctag tagtcgtcaa aggaaataaa gacatagcgt
      121 aaagtataca aaatgacttc cgcggcgcgg gagcggcagg cagcacttgc aaaacgaagc
      181 ccaatgggcc agattacggt caacctcgtc attttattac ggtcgtcagc aagagcggtt
      241 tagaccgtcg ccggcacggg tggctcccga tattcttggc tttcgtgggt ggaggcggcg
      301 tgtgactcct catctttaag tagtggtcgc ttttgctgca tgcttttgat cacctcgtga
      361 ttgatggctg gattctcagc tgtcttctga catctttggg tgcatgggtt gaccggtgtc
      421 gcgggaggca gaccctgttc cgtggaggtg tgtggctgcg gatgcggcga cacggaagaa
      481 gggcgtgcat cacttctctc ttagtcgatt tacgatgcgc ttctgcgcac agctcattca
      541 cccacccgcc accactcctc ttgcacatgt atgcaggtgt cgggccaagt tagtcgcgcg
      601 ctgagtggcc cgaaccccgc atgtggtagt ggaatgcgtg gtcggcagaa gccttgagca
      661 cagcgaaaga agaaaacaag ataagcaggg gatggctgcg gtttgcgcgt gcctttgccg
      721 cgcagcccgt cacaacaata cgtacgatcc ttctgattgt ttgtcgctcg gcagaaaatg
      781 aggagtctag ctctgcaaca caaagaaatt cacaaatgta acaagactct gtgccattat
      841 ttcttatttc ttcctctgtt gtcccaatcg gaaaagctag ggccgtgggg tggccgtaat
      901 cccagacatt ccgggcgctg cctcgccaac gggtggctcc cgaaggactt ggctttcgtg
      961 ggcagggagg gccctgcgct gggttttaga cgtgccgcga gtgctgcctt gattcgaggt
     1021 acacgttaga gatatgaggc acatagcagt tcgctggcac ccccgcgtgt aggcgtgtcc
     1081 tcatccgtgt ttggtttggt acgtcggtta ccggaggggc ctgtgtggct gtcaagcgcg
     1141 acgaagacgg gtgagtgttg cctcgtgcct ccaggtgctt tgatgacgcg cattgcgcgt
     1201 ttcgcttatc caaagaccgc aatcactctc tgaccatgca atcaggttga tagggacgtc
     1261 aaagtgcatt ttatggctta atgctggtcg cgcgggtgta catgtggcgt ctggacggga
     1321 acctcagaac gaagaaaaca agatagggat ggctaacagt tgcacacgtc cctcgtgtgg
     1381 ctgactgaaa gacgtttgcg ccacattaat gtgtaaacat attgagctag tcttacagtt
     1441 attcaggagg aggacaaaat taagtcgtcc gaccgtgtgt tatctaggaa gccatgacgc
     1501 gagacatgtg cctagtatct ctagtacggc tgcgtcttct ttcctccctc tttgaaagga
     1561 agccgtcggt gtccgcgatt ctattctatg cgaaagctgt ggaagatgca ttagtcgcgc
     1621 tttccttgat agttgaccgt cagtggctga ttcagggaaa tcctcacatc atcctgtctt
     1681 tctcacacga cagcctcttc tttcggaata ttcccctcaa cacctaatct gtaaaccctc
     1741 gagcagctga ccatggccaa gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc
     1801 gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt cgtggaggac
     1861 gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt ccaggaccag
     1921 gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc
     1981 gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc catgaccgag
     2041 atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg caactgcgtg
     2101 cacttcgtgg ccgaggagca ggactgacaa ttgggtcacg aactccagca gggctagcgt
     2161 acgcgtacga gagctccgat gccccaattg gaaagatcca agagagacga gtagagattt
     2221 tttttttggg attgatgttt gtcgttcttt gagttgtcgt cgagttacgc cttttgtaag
     2281 aatgttccgc aggagaggag gaggatgggc atgagtgagg gtgagagggc ttgcccgctt
     2341 ttttttttaa aaacgctgaa gacgtggttg tcaaacaaac cccccataga aacgattttg
     2401 ttacggtgcg gtccagacgt cacttgaatg gctccgcgga aaggccaggg agggaagggg
     2461 ggagggagga aacatgaaac atgttgaacg gctcaacagg gtttggggga caagagaggt
     2521 agcgccctga tggactgctc cctcccctcc tttccctcaa tgtctcattc atccatgctt
     2581 cccccttctc tctctcccct ccgttccatc ccccgcgggc gtggtagtgg cgtgatggga
     2641 tccactaaaa tgtacgtgta agaaaagccg gtgagcttac gcttttgtga aagtgggagt
     2701 acgagtgttg tgtgtgtgtg tagtggtttc agaccccaga cagaggcgaa gcagaaaaag
     2761 cagacgatga agacgacgaa gaaatgagca gtctattttt atcgtggaaa cagaagaggt
     2821 gatatcgtct cctgcaggca tgcaagctga tccactagag gccatggcgg ccgcactagt
     2881 ggatcccccg ggatgcatga attcaagctt atcgatatca acaagtttgt acaaaaaagc
     2941 tgaacgagaa acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa
     3001 acagactaca taatactgta aaacacaaca tatccagtca ctatggcggc cgcattaggc
     3061 accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt gagttaggat
     3121 ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc
     3181 accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct
     3241 caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag
     3301 aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct
     3361 catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac
     3421 ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac
     3481 cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa
     3541 aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc
     3601 tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc
     3661 gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt
     3721 caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa
     3781 cagtactgcg atgagtggca gggcggggcg taaagatctg gatccggctt actaaaagcc
     3841 agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata tactgatatg
     3901 tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca gtgacagttg
     3961 acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg gtctggtaag
     4021 cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca
     4081 ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg ctgacgagaa
     4141 caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc cgttatcgtc
     4201 tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg gtgatccccc
     4261 tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg gtggtgcata
     4321 tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg gtctccgtta
     4381 tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc
     4441 tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct gcaggtcgac
     4501 catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc
     4561 taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt cttgtacaaa
     4621 gtggttgata gcttggcgcg cctcatgggc gggtccggcg aggacgcaaa gaatatcaag
     4681 aaaggccctg caccgttcta ccctttggag gacgggaccg cgggagagca gcttcacaag
     4741 gctatgaaac gttacgctct tgttccgggc acgatcgcat tcacggatgc ccacatcgag
     4801 gtcgacatta catatgctga gtacttcgag atgtccgtgc gccttgccga agctatgaag
     4861 cgttacgggc tgaacacgaa ccaccgcatc gtggtctgtt cagagaattc cctacagttc
     4921 tttatgcccg tgttgggggc ccttttcatt ggcgtcgccg tggcccccgc caacgacatt
     4981 tataacgagc gcgagctgct caacagcatg gggatttcgc agccgactgt ggtcttcgtt
     5041 tccaagaaag gtttgcaaaa gatattgaat gtacagaaga aactgcccat tatccagaag
     5101 attatcatta tggactctaa gaccgattac cagggattcc agtcgatgta cactttcgtc
     5161 acgagccatc tcccacccgg gttcaacgag tacgacttcg tgccggagag cttcgaccgc
     5221 gacaagacaa tcgcccttat tatgaactct agtggtagca ccggtttgcc caagggcgtg
     5281 gcgctgcccc atcgtaccgc ctgcgtgcgc ttctcccatg cgcgtgatcc tatcttcggc
     5341 aaccagatca ttccggacac cgcgattctt tcggtcgtgc cgtttcacca tggatttggc
     5401 atgtttacga ctttgggcta cctcatctgc ggcttccggg tggtcctgat gtaccggttt
     5461 gaggaagagc tgtttttgcg cagtctgcag gactacaaga tccagtccgc gctgctcgtc
     5521 cctacactct tttctttctt tgctaagtcc accttaatcg acaagtacga tctgtcaaac
     5581 ctccacgaga tcgcctccgg aggtgcgccc ttatctaagg aagtcgggga ggctgtcgcg
     5641 aagagatttc acctgcccgg catcaggcag ggatatggcc tgaccgagac cacttccgcc
     5701 atcttgatca cgcccgaggg tgacgataaa ccaggcgccg tggggaaagt ggtccctttt
     5761 ttcgaagcaa aggtcgttga cctggacacc ggcaagacgc tcggggtgaa ccaacgaggt
     5821 gagttgtgcg tgcgcggccc tatgattatg agcgggtacg tgaacaatcc cgaagccacc
     5881 aacgcactca ttgataagga cggatggcta cacagcggtg atatcgccta ctgggatgag
     5941 gacgagcact tcttcatcgt agaccggctg aagtcgctca tcaagtacaa ggggtatcag
     6001 gtggcccctg cggagttgga atctattctg ctccaacacc ctaatatctt cgacgccggc
     6061 gtggccggct tgcccgacga tgacgcgggc gagcttcccg ccgcggttgt ggtcctggag
     6121 cacggcaaga cgatgaccga gaaggagatc gtagactacg tggcttccca agtgactacg
     6181 gcgaagaagt tgaggggtgg agtagtgttc gtggatgagg tccccaaggg gctcaccggt
     6241 aagctcgatg cccgtaagat ccgagagatc ttgattaagg ctaagaaggg gggcaagatc
     6301 gccgtgtcgt cgtatcccta cgatgttccc gattatgcct aagagctcga aagatccaag
     6361 agagacgagt agagattttt tttttgggat tgatgtttgt cgttctttga gttgtcgtcg
     6421 agttacgcct tttgtaagaa tgttccgcag gagaggagga ggatgggcat gagtgagggt
     6481 gagagggctt gcccgctttt ttttttaaaa acgctgaaga cgtggttgtc aaacaaaccc
     6541 cccatagaaa cgattttgtt acggtgcggt ccagacgtca cttgaatggc tccgcggaaa
     6601 ggccagggag ggaagggggg agggaggaaa catgaaacat gttgaacggc tcaacagggt
     6661 ttgggggaca agagaggtag cgccctgatg gactgctccc tcccctcctt tccctcaatg
     6721 tctcattcat ccatgcttcc cccttctctc tctcccctcc gttccatccc ccgcgggcgt
     6781 ggtagtggcg tgatgggatc cactaaaatg tacgtgtaag aaaagccggt gagcttacgc
     6841 ttttgtgaaa gtgggagtac gagtgttgtg tgtgtgtgta gtggtttcag accccagaca
     6901 gaggcgaagc agaaaaagca gacgatgaag acgacgaaga aatgagcagt ctatttttat
     6961 cgtggaaaca gaagaggtga tatcgtctcg ttctttgtta tcacctaccc cgcgtgcatg
     7021 tacatgcagc ctttttattt tgtaatcttt cccgaaaaat caaccgccac ctcccccccg
     7081 ccttctctca cccatcatct tctcctgttt atcttctact ttacactaga tcgcatggca
     7141 catctccctc gcaatccatc ggtgcaacca tcatcgatcc cactcctccc tccctccctc
     7201 cctccctcct cgcatctctt ctaagaaatc cgctagctgc gaacgcagct cacctacctc
     7261 atttatcaga gcctcgattc ggtccttttc cttctcccct acctcgccgc aaacagcaac
     7321 agaagcagga gcagcaggag cagcggcacc tgtgtcaaca gcacaagggc aagtacagac
     7381 agaagacgag gcggaggagg aggaagacga tgtgatgtca ttggagctgg ctaaactatt
     7441 agccgttgct ctcaccgcct ttccccaaaa ctcttccacc catcgtgtca ctccttctat
     7501 caggggaacc atccctcttt tcctccccac cttcttcccc ttgctcttct tcttcctttg
     7561 tctctttgtt ccccctcctc tcttcgcctc ctcctccgca tcgtcctctc cttttatcaa
     7621 gtcttgcgtt gcaaaggaag caagctcttt gtgaaggacc gcgcaaggga taggagagtg
     7681 gagagtgagg tgatgcagac aaaccgagga ttcccgggcg tcagataacc taaaatcgac
     7741 tacttcttga ggtggaggag gagggggagg agacggagaa taagtgtgtg tagaaatata
     7801 gcttgctgcc tctgcttcca tcttcgataa aattgctgtt gctgttgctg ttgcggctga
     7861 tatctgctgc ggctgctgct gcacctcctg aacgaagact gcagccgcgt cttcaccctc
     7921 ttggactact ctgccctcct cccttgcctt tacaactcga tcgacgagat tcgtgccagt
     7981 gctgtcatca tcattggaaa gagcaacagc atcaccttaa gattgaatcc tgttgccggt
     8041 cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg
     8101 taatgcatag cttgagtatt ctatagtgtc acctaaatag cttggcgtaa tcatggtcat
     8161 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     8221 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     8281 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     8341 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     8401 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     8461 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     8521 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     8581 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     8641 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     8701 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     8761 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     8821 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     8881 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     8941 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     9001 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     9061 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     9121 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     9181 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     9241 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     9301 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     9361 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     9421 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     9481 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     9541 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     9601 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     9661 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     9721 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     9781 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     9841 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     9901 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     9961 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
    10021 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    10081 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    10141 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    10201 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    10261 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgat gcggtgtgaa
    10321 ataccgcaca gatgcgtaag gagaaaatac cgcatcagga aattgtaagc gttaatattt
    10381 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
    10441 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
    10501 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
    10561 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
    10621 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
    10681 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
    10741 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
    10801 cgctacaggg cgcgtccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
    10861 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
    10921 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgaattgta
    10981 atacgactca ctata
//
