LOCUS       sequence-210266-        9174 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Broad host bacterial expression vector with constitutive PsbA 
            promoter. Expresses mTurquoise2; codon optimized for P. 
            fluorescens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9174)
  AUTHORS   Wilton R, Ahrendt AJ, Shinde S, Sholto-Douglas DJ, Johnson JL, 
            Brennan MB, Kemner KM
  TITLE     A New Suite of Plasmid Vectors for Fluorescence-Based Imaging of 
            Root Colonizing Pseudomonads.
  JOURNAL   Front Plant Sci. 2018 Feb 1;8:2242. doi: 10.3389/fpls.2017.02242. 
            eCollection 2017.
  PUBMED    29449848
REFERENCE   2  (bases 1 to 9174)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci."; date: "2018-02-1"; volume: "8:2242. doi"; pages: " 
            10.3389/fpls.2017.02242. eCollection 2017"
COMMENT     Sequence Label: pSW002-PpsbA_mTurquoise2
FEATURES             Location/Qualifiers
     source          1..9174
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      722..916
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     rep_origin      1625..2170
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     2071..2090
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2287..2304
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(2791..2808)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             3545..3567
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             complement(4522..5172)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     CDS             5278..6477
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             7699..8328
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             join(8757..9174,1..656)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
ORIGIN
        1 tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg gaagggaagg
       61 gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag ttctgccggc
      121 gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg ttaaacacca
      181 cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg acggtatccg
      241 agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg cggccggagt
      301 acatcgagat cgagctagct gattggatgt accgcgagat cacagaaggc aagaacccgg
      361 acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc cgttttctct
      421 accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc aagacgatct
      481 acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg cgcaagctga
      541 tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag gctggcccga
      601 tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt tcctaatgta
      661 cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa ggtctctttc
      721 ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg aacccgtaca
      781 ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact gatataaaag
      841 agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact cttaaaaccc
      901 gcctggcctg tgcataactg tctggccagc gcacagccga agagctgcaa aaagcgccta
      961 cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc gcggccgatg
     1021 gccgctcaaa aatggctggc ctacggccag gcaatctacc agggcgcgga caagccgcgc
     1081 cgtcgccact cgaccgccgg cgctgaggtc tgcctcgtga agaaggtgtt gctgactcat
     1141 accaggcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     1201 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     1261 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     1321 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     1381 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     1441 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     1501 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     1561 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agacccctta ataagatgat
     1621 cttcttgaga tcgttttggt ctgcgcgtaa tctcttgctc tgaaaacgaa aaaaccgcct
     1681 tgcagggcgg tttttcgaag gttctctgag ctaccaactc tttgaaccga ggtaactggc
     1741 ttggaggagc gcagtcacca aaacttgtcc tttcagttta gccttaaccg gcgcatgact
     1801 tcaagactaa ctcctctaaa tcaattacca gtggctgctg ccagtggtgc ttttgcatgt
     1861 ctttccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc ggactgaacg
     1921 gggggttcgt gcatacagtc cagcttggag cgaactgcct acccggaact gagtgtcagg
     1981 cgtggaatga gacaaacgcg gccataacag cggaatgaca ccggtaaacc gaaaggcagg
     2041 aacaggagag cgcacgaggg agccgccagg gggaaacgcc tggtatcttt atagtcctgt
     2101 cgggtttcgc caccactgat ttgagcgtca gatttcgtga tgcttgtcag gggggcggag
     2161 cctatggaaa aacggctttg ccgcggccct ctcacttccc tgttaagtat cttcctggca
     2221 tcttccagga aatctccgcc ccgttcgtaa gccatttccg ctcgccgcag tcgaacgacc
     2281 gagcgtagcg agtcagtgag cgaggaagcg gaatatatcc tgtatcacat attctgctga
     2341 cgcaccggtg cagccttttt tctcctgcca catgaagcac ttcactgaca ccctcatcag
     2401 tgccaacata gtaagccagt atacactccg ctagcgctga ggtctgcctc gtgaagaagg
     2461 tgttgctgac tcataccagg tagtccgagg agctttatgc ttgtaaaccg ttttgtgaaa
     2521 aaatttttaa aataaaaaag gggacctcta gggtccccaa ttaattagta atataatcta
     2581 ttaaaggtca ttcaaaaggt catccaccgg atcaattccc ctgctcgcgc aggctgggtg
     2641 ccaagctctc gggtaacatc aaggcccgat ccttggagcc cttgccctcc cgcacgatga
     2701 tcgtgccgtg atcgaaatcc agatccttga cccgcagttg caaaccctca ctgatccgct
     2761 agtccgaggc ctcgagatgc ttaaagctta gtgatggtga tgatgatgtg cggccgctta
     2821 cttgtacagc tcgtccatgc ccagggtgat gccggccgcg gtcacgaact ccagcagaac
     2881 catgtggtcg cgtttttcgt taggatcttt actgagttta ctttgggtgg agaggtaatg
     2941 gttgtcaggc agcagaacgg ggccgtcgcc gatgggggta ttttgttgat aatggtctgc
     3001 gagttgaaca ccgccgtctt cgatattatg gcggatttta aaattagctt tgatgccgtt
     3061 tttctgtttg tcagcggtga tgtaaacgtt gtcggagaaa tagttgtatt cgagtttgtg
     3121 gcccaggatg ttaccgtctt ccttaaaatc aatgcccttc agttcgatgc ggttgaccag
     3181 ggtatcgcct tcgaatttca cttcggcccg agttttgtag ttgccgtcgt ctttgaagaa
     3241 aatggtgcgt tcttgaacat agccttctgg catggcgctt ttgaagaaat cgtgttgttt
     3301 catatggtcg gggtaacggg caaagcattg gacgccccag ctcaaggtgg tcacgagggt
     3361 aggccaagga acagggagtt tgccggtagt gcagataaat ttcagggtca gtttgccgta
     3421 agttgcatcg ccctcgcctt cgccgctcac ggaaaattta tggccattca cgtcgccatc
     3481 gagttccacg aggattggca caacgccggt aaagagctct tcgcccttgg aattcatatg
     3541 tatatctcct tcttaaagtt aaacaaaatt atttcaagag gatcgatccc ccgtctctct
     3601 aaaattgcag tcatggtaaa atcttggttt atttaattta atcatcaggg actcccaagc
     3661 acattaattc ttctcaaaat agagaattga tagcttgtta ttcaacagta taacatgtct
     3721 tatacgcccg tgtcaaccaa tattcattga gatccacgcg tcgacgtcat atggatccga
     3781 tatcgccgtg gcggccagct tttgccattc tcaccggatt cagtcgtcac tcatggtgat
     3841 ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga
     3901 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag
     3961 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg
     4021 aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg
     4081 ttgtaacact ggcagagcat tacgctgact tgacgggacg gcggctttgt tgaataaatc
     4141 gaacttttgc tgagttgaag gatcagatca cgcatcttcc cgacaacgca gaccgttccg
     4201 tggcaaagca aaagttcaaa atcaccaact ggtccaccta caacaaagct ctcatcaacc
     4261 gtggctccct cactttctgg ctggatgatg gggcgattca ggcctggtat gagtcagcaa
     4321 caccttcttc acgaggcaga cctcagcgcc agaaggccgc cagagaggcc gagcgcggcc
     4381 gtgaggcttg gacgctaggg cagggcatga aaaagcccgt agcgggctgc tacgggcgtc
     4441 tgacgcggtg gaaaggggga ggggatgttg tctacatggc tctgctgtag tgagtgggtt
     4501 gcgctccggc agcggtcctg atcaatcgtc accctttctc ggtccttcaa cgttcctgac
     4561 aacgagcctc cttttcgcca atccatcgac aatcaccgcg agtccctgct cgaacgctgc
     4621 gtccggaccg gcttcgtcga aggcgtctat cgcggcccgc aacagcggcg agagcggagc
     4681 ctgttcaacg gtgccgccgc gctcgccggc atcgctgtcg ccggcctgct cctcaagcac
     4741 ggccccaaca gtgaagtagc tgattgtcat cagcgcattg acggcgtccc cggccgaaaa
     4801 acccgcctcg cagaggaagc gaagctgcgc gtcggccgtt tccatctgcg gtgcgcccgg
     4861 tcgcgtgccg gcatggatgc gcgcgccatc gcggtaggcg agcagcgcct gcctgaagct
     4921 gcgggcattc ccgatcagaa atgagcgcca gtcgtcgtcg gctctcggca ccgaatgcgt
     4981 atgattctcc gccagcatgg cttcggccag tgcgtcgagc agcgcccgct tgttcctgaa
     5041 gtgccagtaa agcgccggct gctgaacccc caaccgttcc gccagtttgc gtgtcgtcag
     5101 accgtctacg ccgacctcgt tcaacaggtc cagggcggca cggatcactg tattcggctg
     5161 caactttgtc atgcttgaca ctttatcact gataaacata atatgtccac caacttatca
     5221 gtgataaaga atccgcgcgt tcaatcggac cagcggaggc tggtccggag gccagacgtg
     5281 aaacccaaca tacccctgat cgtaattctg agcactgtcg cgctcgacgc tgtcggcatc
     5341 ggcctgatta tgccggtgct gccgggcctc ctgcgcgatc tggttcactc gaacgacgtc
     5401 accgcccact atggcattct gctggcgctg tatgcgttgg tgcaatttgc ctgcgcacct
     5461 gtgctgggcg cgctgtcgga tcgtttcggg cggcggccaa tcttgctcgt ctcgctggcc
     5521 ggcgccactg tcgactacgc catcatggcg acagcgcctt tcctttgggt tctctatatc
     5581 gggcggatcg tggccggcat caccggggcg actggggcgg tagccggcgc ttatattgcc
     5641 gatatcactg atggcgatga gcgcgcgcgg cacttcggct tcatgagcgc ctgtttcggg
     5701 ttcgggatgg tcgcgggacc tgtgctcggt gggctgatgg gcggtttctc cccccacgct
     5761 ccgttcttcg ccgcggcagc cttgaacggc ctcaatttcc tgacgggctg tttccttttg
     5821 ccggagtcgc acaaaggcga acgccggccg ttacgccggg aggctctcaa cccgctcgct
     5881 tcgttccggt gggcccgggg catgaccgtc gtcgccgccc tgatggcggt cttcttcatc
     5941 atgcaacttg tcggacaggt gccggccgcg ctttgggtca ttttcggcga ggatcgcttt
     6001 cactgggacg cgaccacgat cggcatttcg cttgccgcat ttggcattct gcattcactc
     6061 gcccaggcaa tgatcaccgg ccctgtagcc gcccggctcg gcgaaaggcg ggcactcatg
     6121 ctcggaatga ttgccgacgg cacaggctac atcctgcttg ccttcgcgac acggggatgg
     6181 atggcgttcc cgatcatggt cctgcttgct tcgggtggca tcggaatgcc ggcgctgcaa
     6241 gcaatgttgt ccaggcaggt ggatgaggaa cgtcaggggc agctgcaagg ctcactggcg
     6301 gcgctcacca gcctgacctc gatcgtcgga cccctcctct tcacggcgat ctatgcggct
     6361 tctataacaa cgtggaacgg gtgggcatgg attgcaggcg ctgccctcta cttgctctgc
     6421 ctgccggcgc tgcgtcgcgg gctttggagc ggcgcagggc aacgagccga tcgctgatcg
     6481 tggaaacgat aggggccgct ctagtactag ctagaactat gatccaaccc ctccgctgct
     6541 atagtgcagt cggcttctga cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca
     6601 agtcctaagt tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt
     6661 gttttagtcg cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca
     6721 agagcgccgc cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga
     6781 ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca
     6841 ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg
     6901 acgttgtgac agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca
     6961 ttgccgagcg catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg
     7021 acaccaccac gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg
     7081 agcgttccct aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg
     7141 tgaagtttgg cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga
     7201 tcgaccagga aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga
     7261 ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg
     7321 gtgccttccg tgaggacgca ttgaccgagg ccgacgccct ggcagccgcc gagaatgaac
     7381 gccaagagga acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac
     7441 cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt
     7501 ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg
     7561 gccggccagc ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt
     7621 tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca
     7681 aatacgcaag gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc
     7741 aagacgacca tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg
     7801 ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa
     7861 ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc
     7921 cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg
     7981 atcaaggcag ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc
     8041 accgccgacc tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa
     8101 gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag
     8161 gcgctggccg ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac
     8221 ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc
     8281 cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta
     8341 aagagaaaat gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca
     8401 gcaaggctgc aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc
     8461 agttgccggc ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca
     8521 ttaccgagct gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa
     8581 atgagtagat gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc
     8641 accgacgccg tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc
     8701 tgggttgtct gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga
     8761 gcggtcgcaa accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg
     8821 agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg
     8881 gtgaatcgtg gcaagcagcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag
     8941 ccggtgcgcc gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc
     9001 cgatgctcta tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc
     9061 gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc
     9121 acgtagaggt ttccgcaggg ccggccggca tggccagtgt gtgggattac gacc
//
