LOCUS       sequence-210274-       10926 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human PCDH7 transcript variant a in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10926)
  AUTHORS   Zhou X, Updegraff BL, Guo Y, Peyton M, Girard L, Larsen JE, Xie XJ, 
            Zhou Y, Hwang TH, Xie Y, Rodriguez-Canales J, Villalobos P, Behrens 
            C, Wistuba II, Minna JD, O'Donnell KA
  TITLE     PROTOCADHERIN 7 Acts through SET and PP2A to Potentiate MAPK 
            Signaling by EGFR and KRAS during Lung Tumorigenesis.
  JOURNAL   Cancer Res. 2017 Jan 1;77(1):187-197. doi: 
            10.1158/0008-5472.CAN-16-1267-T. Epub 2016 Nov 7.
  PUBMED    27821484
REFERENCE   2  (bases 1 to 10926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/0008-5472.CAN-16-1267-T"; journalName: "Cancer Res"; date: 
            "2017-01-1- 1"; volume: "77"; issue: "1"; pages: "187-197"
COMMENT     Sequence Label: pLX303-PCDH7-a
FEATURES             Location/Qualifiers
     source          1..10926
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1710..2210
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2129..2147
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2222..2617
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    2677..2794
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2911..3214
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3215..3418
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3369..3389
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    3441..3465
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6712..6736)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6779..7367
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6832..6852)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7250..7261)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7439..7672
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7750..7871
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7787..7806)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7841..7860
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7911..8046
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8066..8085)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8067..8085)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8095..8112)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8095..8111)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8104..8126)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8253..8708
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8340..8359)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8550..8571
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8734..8838
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8839..8907,8908..9699)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9057..9076)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9870..10458
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10359..10378
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10612..10629
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10746..10767
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10782..10787,10788..10805,10806..10812)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10820..10836
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10825..10847
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10844..10860
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10844..10860
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10879..10899
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10881..10899
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggaccct tgcgcctttt ccaaggcagc cctgggtttg
     1741 cgcagggacg cggctgctct gggcgtggtt ccgggaaacg cagcggcgcc gaccctgggt
     1801 ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat cttcgccgct acccttgtgg
     1861 gccccccggc gacgcttcct gctccgcccc taagtcggga aggttccttg cggttcgcgg
     1921 cgtgccggac gtgacaaacg gaagccgcac gtctcactag taccctcgca gacggacagc
     1981 gccagggagc aatggcagcg cgccgaccgc gatgggctgt ggccaatagc ggctgctcag
     2041 cagggcgcgc cgagagcagc ggccgggaag gggcggtgcg ggaggcgggg tgtggggcgg
     2101 tagtgtgggc cctgttcctg cccgcgcggt gttccgcatt ctgcaagcct ccggagcgca
     2161 cgtcggcagt cggctccctc gttgaccgaa tcaccgacct ctctccccag ggggtaccac
     2221 catggccaag cctttgtctc aagaagaatc caccctcatt gaaagagcaa cggctacaat
     2281 caacagcatc cccatctctg aagactacag cgtcgccagc gcagctctct ctagcgacgg
     2341 ccgcatcttc actggtgtca atgtatatca ttttactggg ggaccttgtg cagaactcgt
     2401 ggtgctgggc actgctgctg ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg
     2461 aaatgagaac aggggcatct tgagcccctg cggacggtgc cgacaggtgc ttctcgatct
     2521 gcatcctggg atcaaagcca tagtgaagga cagtgatgga cagccgacgg cagttgggat
     2581 tcgtgaattg ctgccctctg gttatgtgtg ggagggcctg cagctgcagt agtaagaatt
     2641 ctagatcttg agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
     2701 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
     2761 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
     2821 tccactttgg cgccggctcg agggggcccg gggttattaa tagtaatcaa ttacggggtc
     2881 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     2941 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     3001 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     3061 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     3121 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     3181 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
     3241 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     3301 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
     3361 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct
     3421 ctggctaact gtcgggatca acaagtttgt acaaaaaagc aggctccgcg gccgccccct
     3481 tcaccatgct gaggatgcgg accgcgggat gggcgcgcgg ctggtgcttg ggctgctgcc
     3541 tcctcctgcc gctctcgctc agcctggcgg ccgccaagca gctcctccgg taccggctgg
     3601 ccgaggaggg ccccgccgac gtccgcatcg gcaacgtggc ttcagacctg ggcatcgtga
     3661 ccggatcggg tgaggtgact ttcagcctgg agtccggttc cgagtacctg aagatcgaca
     3721 acctcactgg cgagctgagc acgagcgagc ggcgcatcga ccgcgagaag ctgccccagt
     3781 gtcagatgat cttcgacgag aacgagtgct tcctggactt cgaggtgtcg gtgatcgggc
     3841 cctcgcagag ctgggtggac ctgtttgagg gtcaggtcat cgtgcttgac atcaacgaca
     3901 acacgcccac cttcccgtcg cccgtgctca cgctcacggt ggaggagaat cggccggtgg
     3961 gcacacttta cctgctgccc acagccaccg accgcgactt cggccgcaac ggcatcgagc
     4021 gctacgagct gctccaggag cccggaggcg gcggcagcgg cggcgagagc cggcgcgccg
     4081 gggcggccga cagcgccccc taccccgggg gcggcgggaa cggcgcgagc ggcggcggct
     4141 cgggaggctc caagcggcgg ctggacgcat cagagggcgg cggcggcacc aaccccggcg
     4201 gccgcagcag cgtgttcgag ctgcaggtgg cggacacccc ggacggcgag aagcagccgc
     4261 agctgatcgt gaagggggcg ctggaccgcg agcagcgcga ctcctacgag ctgaccctgc
     4321 gagtgcgcga cggcggcgac ccgcctcgct cctcgcaggc catcctacgg gtcctcatca
     4381 ccgacgtgaa cgacaacagc ccccgcttcg agaagagcgt gtacgaggcc gacttggctg
     4441 agaacagcgc cccggggacc cccatcctgc aactgcgcgc agccgacttg gacgtggggg
     4501 tcaacgggca gatcgaatac gtgttcgggg cggccaccga gtcggtgagg cggctgctgc
     4561 gccttgacga gacgtccggc tggctcagcg tcctgcaccg gatcgaccgc gaggaggtga
     4621 accagctgcg cttcacggtc atggcccgcg accgcgggca gccccccaag accgacaagg
     4681 ccaccgtggt ccttaacatc aaagacgaga acgacaacgt gccgtccatt gaaatccgca
     4741 agattgggcg catccccctc aaggacgggg tggccaacgt ggccgaggac gttctggtcg
     4801 acacccccat cgctctggtg caggtgtccg accgagacca aggcgagaac ggggtggtca
     4861 cctgcaccgt ggtgggcgac gtgcccttcc agctcaagcc agccagcgac accgagggcg
     4921 accagaacaa gaaaaagtac ttcttgcaca cctcgacccc tctggactat gaggccaccc
     4981 gggagttcaa cgtggtcatc gtggcggtgg actcaggcag ccccagcctc tcgagcaaca
     5041 actccctgat tgtcaaggtg ggagacacca acgacaaccc gcccatgttc ggccagtcgg
     5101 tggtggaggt ttacttccct gagaacaaca tcccgggcga gagggtggcc acggtgctgg
     5161 cgacagacgc agacagcggt aagaacgccg agatcgccta ctcgctggac tcctctgtga
     5221 tggggatctt tgccatcgat cccgattctg gggacatcct ggtcaatacc gtgctggacc
     5281 gcgagcagac tgacaggtat gagtttaaag ttaacgccaa agacaaaggc atccccgtgc
     5341 tgcagggcag cactacggtg attgtgcagg tggctgataa aaatgacaat gaccctaagt
     5401 ttatgcagga cgtcttcacc ttttatgtga aagaaaactt gcagcccaac agccctgtgg
     5461 ggatggtcac cgtgatggat gctgacaagg ggcggaatgc agagatgagc ctgtacatag
     5521 aggagaacaa taacattttt tctattgaaa atgacacggg gaccatttac tccacaatgt
     5581 cttttgaccg ggaacatcag accacataca ctttcagagt caaggctgtg gatgggggag
     5641 atcctcccag atctgccaca gctacagtct cgctttttgt gatggatgaa aatgacaatg
     5701 ctcccacagt tacccttccc aaaaacattt cctacacttt actgccacct tcgagtaatg
     5761 tcaggacagt agtagctaca gtgttggcaa cagacagtga tgatggcatc aatgcagacc
     5821 tgaactacag cattgtggga ggaaatccct tcaagctgtt tgaaattgat cccactagtg
     5881 gtgtggtttc cttagtggga aaactcaccc aaaagcatta tggcttgcac aggttggtgg
     5941 tgcaagtgaa tgacagtggg cagccttccc agtccaccac gactctggtg cacgtgtttg
     6001 tcaatgaaag tgtttctaat gcaactgcga ttgactccca gatagctaga agtttgcaca
     6061 tcccactcac ccaggatata gctggtgacc caagctatga aattagcaaa cagagactca
     6121 gtattgtcat tggcgtggtt gctggcatta tgacggtgat tctaatcatc ttaattgtag
     6181 tgatggcaag gtactgcagg tccaaaaata aaaatggcta tgaagccggc aaaaaagatc
     6241 acgaagactt ttttacaccc caacagcatg acaaatctaa aaagcctaaa aaggacaaga
     6301 aaaacaaaaa atctaagcag cctctctaca gcagcattgt cactgtggag gcttctaagc
     6361 caaatggaca gaggtatgat agtgtcaatg agaagctgtc agacagccca agcatggggc
     6421 gatacaggtc cgttaatggt gggcccggca gtcctgacct ggcaaggcat tacaaatcta
     6481 gttccccatt gcctactgtt cagcttcatc cccagtcacc aactgcagga aaaaaacacc
     6541 aggccgtaca agatctacca ccagccaaca catttgtggg agcaggagac aacatttcaa
     6601 ttggatcaga tcactgctct gagtacagct gtcaaaccaa taacaagtac agcaaacaga
     6661 tgcgtctaca tccatacatt actgtgtttg gctgaaaggg tgggcgcgcc gacccagctt
     6721 tcttgtacaa agtggtttag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa
     6781 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     6841 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     6901 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     6961 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     7021 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     7081 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     7141 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     7201 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     7261 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     7321 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcgtc gactttaaga
     7381 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     7441 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     7501 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     7561 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     7621 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtacgtat
     7681 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     7741 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     7801 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     7861 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc
     7921 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     7981 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     8041 gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
     8101 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     8161 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     8221 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
     8281 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     8341 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     8401 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     8461 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     8521 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     8581 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     8641 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     8701 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     8761 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     8821 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     8881 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     8941 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     9001 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     9061 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     9121 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     9181 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     9241 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     9301 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     9361 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     9421 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     9481 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     9541 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     9601 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     9661 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     9721 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     9781 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     9841 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     9901 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     9961 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
    10021 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
    10081 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
    10141 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
    10201 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
    10261 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
    10321 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
    10381 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
    10441 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
    10501 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
    10561 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
    10621 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
    10681 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
    10741 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
    10801 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
    10861 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa
    10921 gcttaa
//
