LOCUS       sequence-210280-        8508 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Wild type Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag
            tag for protein expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8508)
  AUTHORS   Rousseau BA, Hou Z, Gramelspacher MJ, Zhang Y
  TITLE     Programmable RNA Cleavage and Recognition by a Natural CRISPR-Cas9 
            System from Neisseria meningitidis.
  JOURNAL   Mol Cell. 2018 Mar 1;69(5):906-914.e4. doi: 
            10.1016/j.molcel.2018.01.025. Epub 2018 Feb 15.
  PUBMED    29456189
REFERENCE   2  (bases 1 to 8508)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2018.01.025"; journalName: "Mol Cell"; date: 
            "2018-03-1- 1"; volume: "69"; issue: "5"; pages: "906-914.e4"
COMMENT     Sequence Label: pET28b-NmCas9-Flag
FEATURES             Location/Qualifiers
     source          1..8508
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     74..93
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     177..196
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        177..195
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    196..220
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             235..257
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             264..3509
                     /codon_start=1
                     /product="Cas9 endonuclease from the Neisseria meningitidis
                     Type II CRISPR/Cas system (Esvelt et al., 2013)"
                     /label=NmCas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MAAFKPNSINYILGLDIGIASVGWAMVEIDEEENPIRLIDLGVRV
                     FERAEVPKTGDSLAMARRLARSVRRLTRRRAHRLLRTRRLLKREGVLQAANFDENGLIK
                     SLPNTPWQLRAAALDRKLTPLEWSAVLLHLIKHRGYLSQRKNEGETADKELGALLKGVA
                     GNAHALQTGDFRTPAELALNKFEKESGHIRNQRSDYSHTFSRKDLQAELILLFEKQKEF
                     GNPHVSGGLKEGIETLLMTQRPALSGDAVQKMLGHCTFEPAEPKAAKNTYTAERFIWLT
                     KLNNLRILEQGSERPLTDTERATLMDEPYRKSKLTYAQARKLLGLEDTAFFKGLRYGKD
                     NAEASTLMEMKAYHAISRALEKEGLKDKKSPLNLSPELQDEIGTAFSLFKTDEDITGRL
                     KDRIQPEILEALLKHISFDKFVQISLKALRRIVPLMEQGKRYDEACAEIYGDHYGKKNT
                     EEKIYLPPIPADEIRNPVVLRALSQARKVINGVVRRYGSPARIHIETAREVGKSFKDRK
                     EIEKRQEENRKDREKAAAKFREYFPNFVGEPKSKDILKLRLYEQQHGKCLYSGKEINLG
                     RLNEKGYVEIDHALPFSRTWDDSFNNKVLVLGSENQNKGNQTPYEYFNGKDNSREWQEF
                     KARVETSRFPRSKKQRILLQKFDEDGFKERNLNDTRYVNRFLCQFVADRMRLTGKGKKR
                     VFASNGQITNLLRGFWGLRKVRAENDRHHALDAVVVACSTVAMQQKITRFVRYKEMNAF
                     DGKTIDKETGEVLHQKTHFPQPWEFFAQEVMIRVFGKPDGKPEFEEADTLEKLRTLLAE
                     KLSSRPEAVHEYVTPLFVSRAPNRKMSGQGHMETVKSAKRLDEGVSVLRVPLTQLKLKD
                     LEKMVNREREPKLYEALKARLEAHKDDPAKAFAEPFYKYDKAGNRTQQVKAVRVEQVQK
                     TGVWVRNHNGIADNATMVRVDVFEKGDKYYLVPIYSWQVAKGILPDRAVVQGKDEEDWQ
                     LIDDSFNFKFSLHPNDLVEVITKKARMFGYFASCHRGTGNINIRIHDLDHKIGKNGILE
                     GIGVKTALSFQKYQIDELGKEIRPCRLKKRPPVR"
     CDS             3510..3533
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3546..3566
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     primer_bind     complement(3619..3637)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      3633..3680
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      3717..4172
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3804..3823)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4014..4035
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(4264..5079)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(4264..5079)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4987..5006
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     5071..5090
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      5201..5789
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5690..5709
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5943..5960
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5975..6114
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6040..6059)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6200..6222
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(6216..6407)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(7216..8298)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     8260..8279
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(8299..8376)
                     /gene="lacI"
                     /label=lacI promoter
ORIGIN
        1 cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc
       61 gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg
      121 tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat
      181 acgactcact ataggggaat tgtgagcgga taacaattcc cctctagaaa taattttgtt
      241 taactttaag aaggagatat accatggctg ccttcaaacc taattcaatc aactacatcc
      301 tcggcctcga tatcggcatc gcatccgtcg gctgggcgat ggtagaaatt gacgaagaag
      361 aaaaccccat ccgcctgatt gatttgggcg tgcgcgtatt tgagcgtgcc gaagtaccga
      421 aaacaggcga ctcccttgcc atggcaaggc gtttggcgcg cagtgttcgc cgcctgaccc
      481 gccgtcgcgc ccaccgcctg cttcggaccc gccgcctatt gaaacgcgaa ggcgtattac
      541 aagccgccaa ttttgacgaa aacggcttga ttaaatcctt accgaataca ccatggcaac
      601 ttcgcgcagc cgcattagac cgcaaactga cgcctttaga gtggtcggca gtcttgttgc
      661 atttaatcaa acatcgcggc tatttatcgc aacggaaaaa cgagggcgaa actgccgata
      721 aggagcttgg cgctttgctt aaaggcgtag ccggcaatgc ccatgcctta cagacaggcg
      781 atttccgcac accggccgaa ttggctttaa ataaatttga gaaagaaagc ggccatatcc
      841 gcaatcagcg cagcgattat tcgcatacgt tcagccgcaa agatttacag gcggagctga
      901 ttttgctgtt tgaaaaacaa aaagaatttg gcaatccgca tgtttcaggc ggccttaaag
      961 aaggtattga aaccctactg atgacgcaac gccctgccct gtccggcgat gccgttcaaa
     1021 aaatgttggg gcattgcacc ttcgaaccgg cagagccgaa agccgctaaa aacacctaca
     1081 cagccgaacg tttcatctgg ctgaccaagc tgaacaacct gcgtatttta gagcaaggca
     1141 gcgagcggcc attgaccgat accgaacgcg ccacgcttat ggacgagcca tacagaaaat
     1201 ccaaactgac ttacgcacaa gcccgtaagc tgctgggttt agaagatacc gcctttttca
     1261 aaggcttgcg ctatggtaaa gacaatgccg aagcctcaac attgatggaa atgaaggcct
     1321 accatgccat cagccgtgca ctggaaaaag aaggattgaa agacaaaaaa tccccattaa
     1381 acctttctcc cgaattacaa gacgaaatcg gcacggcatt ctccctgttc aaaaccgatg
     1441 aagacattac aggccgtctg aaagaccgta tacagcccga aatcttagaa gcgctgttga
     1501 aacacatcag cttcgataag ttcgtccaaa tttccttgaa agcattgcgc cgaattgtgc
     1561 ctctaatgga acaaggcaaa cgttacgatg aagcctgcgc cgaaatctac ggagaccatt
     1621 acggcaagaa gaatacggaa gaaaagattt atctgccgcc gattcccgcc gacgaaatcc
     1681 gcaaccccgt cgtcttgcgc gccttatctc aagcacgtaa ggtcattaac ggcgtggtac
     1741 gccgttacgg ctccccagct cgtatccata ttgaaactgc aagggaagta ggtaaatcgt
     1801 ttaaagaccg caaagaaatt gagaaacgcc aagaagaaaa ccgcaaagac cgggaaaaag
     1861 ccgccgccaa attccgagag tatttcccca attttgtcgg agaacccaaa tccaaagata
     1921 ttctgaaact gcgcctgtac gagcaacaac acggcaaatg cctgtattcg ggcaaagaaa
     1981 tcaacttagg ccgtctgaac gaaaaaggct atgtcgaaat cgaccatgcc ctgccgttct
     2041 cgcgcacatg ggacgacagt ttcaacaata aagtactggt attgggcagc gaaaaccaaa
     2101 acaaaggcaa tcaaacccct tacgaatact tcaacggcaa agacaacagc cgcgaatggc
     2161 aggaatttaa agcgcgtgtc gaaaccagcc gtttcccgcg cagtaaaaaa caacggattc
     2221 tgctgcaaaa attcgatgaa gacggcttta aagaacgcaa tctgaacgac acgcgctacg
     2281 tcaaccgttt cctgtgtcaa tttgttgccg accgtatgcg gctgacaggt aaaggcaaga
     2341 aacgtgtctt tgcatccaac ggacaaatta ccaatctgtt gcgcggcttt tggggattgc
     2401 gcaaagtgcg tgcggaaaac gaccgccatc acgccttgga cgccgtcgtc gttgcctgct
     2461 cgaccgttgc catgcagcag aaaattaccc gttttgtacg ctataaagag atgaacgcgt
     2521 ttgacggtaa aaccatagac aaagaaacag gagaagtgct gcatcaaaaa acacacttcc
     2581 cacaaccttg ggaatttttc gcacaagaag tcatgattcg cgtcttcggc aaaccggacg
     2641 gcaaacccga attcgaagaa gccgataccc tagaaaaact gcgcacgttg cttgccgaaa
     2701 aattatcatc tcgccccgaa gccgtacacg aatacgttac gccactgttt gtttcacgcg
     2761 cgcccaatcg gaagatgagc gggcaagggc atatggagac cgtcaaatcc gccaaacgac
     2821 tggacgaagg cgtcagcgtg ttgcgcgtac cgctgacaca gttaaaactg aaagacttgg
     2881 aaaaaatggt caatcgggag cgcgaaccta agctatacga agcactgaaa gcacggctgg
     2941 aagcacataa agacgatcct gccaaagcct ttgccgagcc gttttacaaa tacgataaag
     3001 caggcaaccg cacccaacag gtaaaagccg tacgcgtaga gcaagtacag aaaaccggcg
     3061 tatgggtgcg caaccataac ggtattgccg acaacgcaac catggtgcgc gtagatgtgt
     3121 ttgagaaagg cgacaagtat tatctggtac cgatttacag ttggcaggta gcgaaaggga
     3181 ttttgccgga tagggctgtt gtacaaggaa aagatgaaga agattggcaa cttattgatg
     3241 atagtttcaa ctttaaattc tcattacacc ctaatgattt agtcgaggtt ataacaaaaa
     3301 aagctagaat gtttggttac tttgccagct gccatcgagg cacaggtaat atcaatatac
     3361 gcattcatga tcttgatcat aaaattggca aaaatggaat actggaaggt atcggcgtca
     3421 aaaccgccct ttcattccaa aaataccaaa ttgacgaact gggcaaagaa atcagaccat
     3481 gccgtctgaa aaaacgcccg cctgtccgtg attataaaga tgatgatgat aaatgagcac
     3541 tcgagcacca ccaccaccac caccactgag atccggctgc taacaaagcc cgaaaggaag
     3601 ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac
     3661 gggtcttgag gggttttttg ctgaaaggag gaactatatc cggattggcg aatgggacgc
     3721 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     3781 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     3841 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     3901 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     3961 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     4021 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     4081 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     4141 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
     4201 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgaattaa
     4261 ttcttagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc
     4321 aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt
     4381 ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca
     4441 acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac
     4501 gactgaatcc ggtgagaatg gcaaaagttt atgcatttct ttccagactt gttcaacagg
     4561 ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga
     4621 ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat
     4681 cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg
     4741 atattcttct aatacctgga atgctgtttt cccggggatc gcagtggtga gtaaccatgc
     4801 atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca
     4861 gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag
     4921 aaacaactct ggcgcatcgg gcttcccata caatcgatag attgtcgcac ctgattgccc
     4981 gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg
     5041 cggcctagag caagacgttt cccgttgaat atggctcata acaccccttg tattactgtt
     5101 tatgtaagca gacagtttta ttgttcatga ccaaaatccc ttaacgtgag ttttcgttcc
     5161 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     5221 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     5281 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     5341 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     5401 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     5461 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     5521 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     5581 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     5641 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     5701 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     5761 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     5821 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     5881 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     5941 gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc
     6001 atctgtgcgg tatttcacac cgcaatggtg cactctcagt acaatctgct ctgatgccgc
     6061 atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga
     6121 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     6181 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     6241 aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg
     6301 tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt
     6361 ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg
     6421 taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg
     6481 atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg
     6541 gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt
     6601 aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac
     6661 ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag
     6721 accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc
     6781 tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc
     6841 ctcaacgaca ggagcacgat catgcgcacc cgtggggccg ccatgccggc gataatggcc
     6901 tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc
     6961 aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc
     7021 tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag
     7081 acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg
     7141 ttgaaggctc tcaagggcat cggtcgagat cccggtgcct aatgagtgag ctaacttaca
     7201 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     7261 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca gggtggtttt
     7321 tcttttcacc agtgagacgg gcaacagctg attgcccttc accgcctggc cctgagagag
     7381 ttgcagcaag cggtccacgc tggtttgccc cagcaggcga aaatcctgtt tgatggtggt
     7441 taacggcggg atataacatg agctgtcttc ggtatcgtcg tatcccacta ccgagatatc
     7501 cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg ccatctgatc
     7561 gttggcaacc agcatcgcag tgggaacgat gccctcattc agcatttgca tggtttgttg
     7621 aaaaccggac atggcactcc agtcgccttc ccgttccgct atcggctgaa tttgattgcg
     7681 agtgagatat ttatgccagc cagccagacg cagacgcgcc gagacagaac ttaatgggcc
     7741 cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc ccagtcgcgt
     7801 accgtcttca tgggagaaaa taatactgtt gatgggtgtc tggtcagaga catcaagaaa
     7861 taacgccgga acattagtgc aggcagcttc cacagcaatg gcatcctggt catccagcgg
     7921 atagttaatg atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg ccgctttaca
     7981 ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg ctggcaccca gttgatcggc
     8041 gcgagattta atcgccgcga caatttgcga cggcgcgtgc agggccagac tggaggtggc
     8101 aacgccaatc agcaacgact gtttgcccgc cagttgttgt gccacgcggt tgggaatgta
     8161 attcagctcc gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa cgtggctggc
     8221 ctggttcacc acgcgggaaa cggtctgata agagacaccg gcatactctg cgacatcgta
     8281 taacgttact ggtttcacat tcaccaccct gaattgactc tcttccgggc gctatcatgc
     8341 cataccgcga aaggttttgc gccattcgat ggtgtccggg atctcgacgc tctcccttat
     8401 gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg
     8461 caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggcca
//
