LOCUS       sequence-210336-        7135 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GsX chimera with G-alpha-q C-terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7135)
  AUTHORS   Ballister ER, Rodgers J, Martial F, Lucas RJ
  TITLE     A live cell assay of GPCR coupling allows identification of 
            optogenetic tools for controlling Go and Gi signaling.
  JOURNAL   BMC Biol. 2018 Jan 16;16(1):10. doi: 10.1186/s12915-017-0475-2.
  PUBMED    29338718
REFERENCE   2  (bases 1 to 7135)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2018-01-16"; volume: "16(1):10. doi"; pages: " 
            10.1186/s12915-017-0475-2"
COMMENT     Sequence Label: pEM705 Gsq
FEATURES             Location/Qualifiers
     source          1..7135
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          138..1154
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1078..1100
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1162..1181
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1224..1233
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2511..2530)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2576..2631
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2630..2649)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     protein_bind    2995..3028
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    3041..3162
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3078..3097)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3132..3151
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3181..3201)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3181..3199)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3220..3236)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3220..3236)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3233..3255)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3244..3260
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3268..3274,3275..3292,3293..3298))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3313..3334
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3451..3468)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3622..4210)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3702..3721)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4381..5172,5173..5241))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5004..5023
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5242..5346)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    5419..5467
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    5679..5712
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             5717..6316
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5717..5736)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6213..6233
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(6452..6469)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6458..6569
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        6714..7017
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ggcggggcga ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg
       61 gcgcgctccg aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc
      121 gaagcgcgcg gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg
      181 ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc
      241 gggacggccc ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt
      301 ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc ggggggagcg
      361 gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc
      421 ccggcggctg tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg
      481 aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag
      541 gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc
      601 tgcaaccccc cctgcacccc cctccccgag ttgctgagca cggcccggct tcgggtgcgg
      661 ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg
      721 gggtgccggg cggggcgggg ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg
      781 gcccccggag cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt
      841 aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg
      901 ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga
      961 aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc
     1021 agcctcgggg ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt
     1081 tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt catgccttct
     1141 tctttttcct acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca
     1201 aagaattgct cgaccaccgg cgcgccacca tgggctgcct cgggaacagt aagaccgagg
     1261 accagcgcaa cgaggagaag gcgcagcgtg aggccaacaa aaagatcgag aagcagctgc
     1321 agaaggacaa gcaggtctac cgggccacgc accgcctgct gctgctgggt gctggagaat
     1381 ctggtaaaag caccattgtg aagcagatga ggatcctgca tgttaatggg tttaatggag
     1441 agggcggcga agaggacccg caggctgcaa ggagcaacag cgatggtgag aaggcaacca
     1501 aagtgcagga catcaaaaac aacctgaaag aggcgattga aaccattgtg gccgccatga
     1561 gcaacctggt gccccccgtg gagctggcca accccgagaa ccagttcaga gtggactaca
     1621 ttctgagtgt gatgaacgtg cctgactttg acttccctcc cgaattctat gagcatgcca
     1681 aggctctgtg ggaggatgaa ggagtgcgtg cctgctacga acgctccaac gagtaccagc
     1741 tgattgactg tgcccagtac ttcctggaca agatcgacgt gatcaagcag gctgactatg
     1801 tgccgagcga tcaggacctg cttcgctgcc gtgtcctgac ttctggaatc tttgagacca
     1861 agttccaggt ggacaaagtc aacttccaca tgtttgacgt gggtggccag cgcgatgaac
     1921 gccgcaagtg gatccagtgc ttcaacgatg tgactgccat catcttcgtg gtggccagca
     1981 gcagctacaa catggtcatc cgggaggaca accagaccaa ccgcctgcag gaggctctga
     2041 acctcttcaa gagcatctgg aacaacagat ggctgcgcac catctctgtg atcctgttcc
     2101 tcaacaagca agatctgctc gctgagaaag tccttgctgg gaaatcgaag attgaggact
     2161 actttccaga atttgctcgc tacactactc ctgaggatgc tactcccgag cccggagagg
     2221 acccacgcgt gacccgggcc aagtacttca ttcgagatga gtttctgagg atcagcactg
     2281 ccagtggaga tgggcgtcac tactgctacc ctcatttcac ctgcgctgtg gacactgaga
     2341 acatccgccg tgtgttcaac gactgccgtg acaccatcct ccagttgaac ctgaaggagt
     2401 acaatctggt ctgactcgag gcggccgcaa ttcactcctc aggtgcaggc tgcctatcag
     2461 aaggtggtgg ctggtgtggc caatgccctg gctcacaaat accactgaga tctttttccc
     2521 tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa
     2581 aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg
     2641 acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca
     2701 acatatgccc atatgctggc tgccatgaac aaaggttggc tataaagagg tcatcagtat
     2761 atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag
     2821 atttttttta tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta
     2881 catgttttac tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct
     2941 cttctcttat ggagatccct cgacctgcac cgtcgagcta gcatatggat ccatataact
     3001 tcgtataatg tatgctatac gaagttatta cgataccgtc aacttgttta ttgcagctta
     3061 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     3121 gcattctagt tgtggtttgt ccaaactcat caatgtatct taggtaccca gcttttgttc
     3181 cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     3241 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     3301 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     3361 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     3421 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     3481 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     3541 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     3601 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     3661 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     3721 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     3781 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     3841 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     3901 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     3961 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     4021 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     4081 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     4141 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     4201 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     4261 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     4321 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     4381 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     4441 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     4501 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     4561 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     4621 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     4681 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     4741 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     4801 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     4861 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     4921 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     4981 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     5041 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     5101 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     5161 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     5221 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     5281 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     5341 tccgcgcaca tttccccgaa aagtgccaag cttaataaac acggaaggag acaataccgg
     5401 aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggg tgttgggtcg
     5461 tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga
     5521 ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg
     5581 ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat agccactggc
     5641 cccgtgggtt agggacgggg tcccccattc gagaataaat aacttcgtat aaagtatcct
     5701 atacgaagtt atcaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     5761 cgtcccccgg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     5821 caccgtcgac ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     5881 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
     5941 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     6001 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     6061 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
     6121 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     6181 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     6241 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     6301 caagcccggt gcctgacgcc cgccccacga cccgcagcgc ccgaccgaaa ggagcgcacg
     6361 accccatggc tccgaccgaa gccacccggg gcggccccgc cgaccccgca cccgcccccg
     6421 aggcccaccg actctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca
     6481 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     6541 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     6601 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag cactagttat
     6661 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     6721 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     6781 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     6841 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     6901 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     6961 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc
     7021 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt
     7081 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg ggggg
//
