LOCUS       sequence-210374-        1959 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid expresses mCherry fluorescent protein under endogenous 
            EF1a promoter. It can be used to transfect ""Capsaspora owczarzaki""
            cells and visualize them by fluorescence microscopy..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Parra-Acero H, Ros-Rocher N, Perez-Posada A, Kozyczkowska A, 
            Sanchez-Pons N, Nakata A, Suga H, Najle SR, Ruiz-Trillo I
  TITLE     Transfection of Capsaspora owczarzaki, a close unicellular relative 
            of animals.
  JOURNAL   Development. 2018 May 11. pii: dev.162107. doi: 10.1242/dev.162107.
  PUBMED    29752387
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development. 2018 May 11. pii: dev.162107. doi: 
            10.1242/dev.162107."
COMMENT     Sequence Label: pONSY-mCherry
FEATURES             Location/Qualifiers
     source          1..1959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             929..1639
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1074..1092)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1342..1361
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1536..1559
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 aaatgcacag ttagcaacga ccgacaatca gcaacctcca aactctcagt ttttttcccc
       61 caatcgttgt cgatttagct tttgatgctg cattttgaaa tagaacgaca accgaatttg
      121 ggagagagca ctcttttaca ggtttgtgaa aagaaaaaaa atcgaacgtg acttgcttcc
      181 atcttccttc tcgaagccga tctcggccca tccccgcttg tcccctcctc ccctcctcgc
      241 aatccgctag acagtgtctc tgcacactgg attgcggtgc gcgggccatc cgatgcccct
      301 ttcctcggcc tggagatgga cgtgaaagtg gaaaacgaaa aacgcgaaac agaaacatgt
      361 ggttcttttt tttttctttt tctttttcgc cttcgggctt ttctctctcg cttcctcttc
      421 tccgctctct tcttcgcgct cttttgcctc ttgcgtgaga tgctcgtctt gcaatcgtct
      481 gccactgctg cgctgtcacg atcgatcgca tcggacaagc cgtttctgtt ttgaaattta
      541 gaacgttctg gaaaacaaca aaaaattgcc tttttttcac ttttttcttt tttcgttttt
      601 ttccctcttc aacacttcct ccaactgtcg ttctgcgttt gttcttcgct ctcgtcgtgc
      661 acactcggat taggatccat tcatccccat ccccttgcgc attgccaaaa acgagcgtcg
      721 aaacgcgaac gtcagcgtgg ggtgttgacg ccgaacccca cgagtgtttt tttttaaaat
      781 gctgtttcat cgttttcttc cttctcccca cgtgcaatcc tgatttaaat aattgtcgct
      841 ttttgatttg gaaatgctca ctcttccatt tttttattat ttaacacaca cagaacaacc
      901 ttcacaggat cccgggacta gtgatatcat ggtgagcaag ggcgaggagg acaacatggc
      961 catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga
     1021 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
     1081 gaaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat
     1141 gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc
     1201 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac
     1261 cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg
     1321 caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc
     1381 ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa
     1441 gctgaaggac ggcggccact acgacgccga ggtcaagacc acctacaagg ccaagaagcc
     1501 cgtgcagctg cccggcgcct acaacgtcaa catcaagctg gacatcacct cccacaacga
     1561 ggactacacc atcgtggaac agtacgagcg cgccgagggc cgccactcca ccggcggcat
     1621 ggacgagctg tacaagtaaa ttttgtgttt gccaagacac atttgtttat ttcaatcatt
     1681 tctaagacca aaaagggatt tcatgatttt tgttgcttgc attgaagttt tttttggttg
     1741 tcattttttt tgcgttggtt atgagcgcaa tatattccat tatggcaaga aacacgacga
     1801 gttgttttta cagtggaggg atgagcaagg aatggggaaa tggagaacaa gtggaatgaa
     1861 atggtttgac tttgtgcggc gaaagtgttg ccattttcaa tcatgctgcg cctggcacct
     1921 agatgcgtgt gattgctcat tgctagtgct gttctcacc
//
