LOCUS       sequence-210376-        1965 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid expresses Venus fluorescent protein under endogenous 
            EF1a promoter. It can be used to transfect ""Capsaspora owczarzaki""
            cells and visualize them by fluorescence microscopy..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Parra-Acero H, Ros-Rocher N, Perez-Posada A, Kozyczkowska A, 
            Sanchez-Pons N, Nakata A, Suga H, Najle SR, Ruiz-Trillo I
  TITLE     Transfection of Capsaspora owczarzaki, a close unicellular relative 
            of animals.
  JOURNAL   Development. 2018 May 11. pii: dev.162107. doi: 10.1242/dev.162107.
  PUBMED    29752387
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development. 2018 May 11. pii: dev.162107. doi: 
            10.1242/dev.162107."
COMMENT     Sequence Label: pONSY-Venus
FEATURES             Location/Qualifiers
     source          1..1965
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(929..931,932..934,935..1648)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(974..995)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1235..1254)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1582..1603
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 aaatgcacag ttagcaacga ccgacaatca gcaacctcca aactctcagt ttttttcccc
       61 caatcgttgt cgatttagct tttgatgctg cattttgaaa tagaacgaca accgaatttg
      121 ggagagagca ctcttttaca ggtttgtgaa aagaaaaaaa atcgaacgtg acttgcttcc
      181 atcttccttc tcgaagccga tctcggccca tccccgcttg tcccctcctc ccctcctcgc
      241 aatccgctag acagtgtctc tgcacactgg attgcggtgc gcgggccatc cgatgcccct
      301 ttcctcggcc tggagatgga cgtgaaagtg gaaaacgaaa aacgcgaaac agaaacatgt
      361 ggttcttttt tttttctttt tctttttcgc cttcgggctt ttctctctcg cttcctcttc
      421 tccgctctct tcttcgcgct cttttgcctc ttgcgtgaga tgctcgtctt gcaatcgtct
      481 gccactgctg cgctgtcacg atcgatcgca tcggacaagc cgtttctgtt ttgaaattta
      541 gaacgttctg gaaaacaaca aaaaattgcc tttttttcac ttttttcttt tttcgttttt
      601 ttccctcttc aacacttcct ccaactgtcg ttctgcgttt gttcttcgct ctcgtcgtgc
      661 acactcggat taggatccat tcatccccat ccccttgcgc attgccaaaa acgagcgtcg
      721 aaacgcgaac gtcagcgtgg ggtgttgacg ccgaacccca cgagtgtttt tttttaaaat
      781 gctgtttcat cgttttcttc cttctcccca cgtgcaatcc tgatttaaat aattgtcgct
      841 ttttgatttg gaaatgctca ctcttccatt tttttattat ttaacacaca cagaacaacc
      901 ttcacaggat cccgggacta gtgatatcat ggtgagcaag ggcgaggagc tgttcaccgg
      961 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     1021 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagctga tctgcaccac
     1081 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgggctacg gcctgcagtg
     1141 cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     1201 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     1261 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     1321 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     1381 ctatatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga tccgccacaa
     1441 catcgaggac ggcggcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     1501 cggccccgtg ctgctgcccg acaaccacta cctgagctac cagtccgccc tgagcaaaga
     1561 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     1621 tctcggcatg gacgagctgt acaagtaaat tttgtgtttg ccaagacaca tttgtttatt
     1681 tcaatcattt ctaagaccaa aaagggattt catgattttt gttgcttgca ttgaagtttt
     1741 ttttggttgt catttttttt gcgttggtta tgagcgcaat atattccatt atggcaagaa
     1801 acacgacgag ttgtttttac agtggaggga tgagcaagga atggggaaat ggagaacaag
     1861 tggaatgaaa tggtttgact ttgtgcggcg aaagtgttgc cattttcaat catgctgcgc
     1921 ctggcaccta gatgcgtgtg attgctcatt gctagtgctg ttctc
//
