LOCUS       sequence-210383-        2349 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid expresses Venus fluorescent protein fused to endogenous
            Histone H2B (H2B) gene of Capsaspora (CAOG_01818). It can be used to
            transfect Capsaspora cells and visualize nucleus in vivo..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Parra-Acero H, Ros-Rocher N, Perez-Posada A, Kozyczkowska A, 
            Sanchez-Pons N, Nakata A, Suga H, Najle SR, Ruiz-Trillo I
  TITLE     Transfection of Capsaspora owczarzaki, a close unicellular relative 
            of animals.
  JOURNAL   Development. 2018 May 11. pii: dev.162107. doi: 10.1242/dev.162107.
  PUBMED    29752387
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development. 2018 May 11. pii: dev.162107. doi: 
            10.1242/dev.162107."
COMMENT     Sequence Label: pONSY-CoH2B:Venus
FEATURES             Location/Qualifiers
     source          1..2349
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1310..1312,1313..1315,1316..2029)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1355..1376)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1616..1635)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1963..1984
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 aaatgcacag ttagcaacga ccgacaatca gcaacctcca aactctcagt ttttttcccc
       61 caatcgttgt cgatttagct tttgatgctg cattttgaaa tagaacgaca accgaatttg
      121 ggagagagca ctcttttaca ggtttgtgaa aagaaaaaaa atcgaacgtg acttgcttcc
      181 atcttccttc tcgaagccga tctcggccca tccccgcttg tcccctcctc ccctcctcgc
      241 aatccgctag acagtgtctc tgcacactgg attgcggtgc gcgggccatc cgatgcccct
      301 ttcctcggcc tggagatgga cgtgaaagtg gaaaacgaaa aacgcgaaac agaaacatgt
      361 ggttcttttt tttttctttt tctttttcgc cttcgggctt ttctctctcg cttcctcttc
      421 tccgctctct tcttcgcgct cttttgcctc ttgcgtgaga tgctcgtctt gcaatcgtct
      481 gccactgctg cgctgtcacg atcgatcgca tcggacaagc cgtttctgtt ttgaaattta
      541 gaacgttctg gaaaacaaca aaaaattgcc tttttttcac ttttttcttt tttcgttttt
      601 ttccctcttc aacacttcct ccaactgtcg ttctgcgttt gttcttcgct ctcgtcgtgc
      661 acactcggat taggatccat tcatccccat ccccttgcgc attgccaaaa acgagcgtcg
      721 aaacgcgaac gtcagcgtgg ggtgttgacg ccgaacccca cgagtgtttt tttttaaaat
      781 gctgtttcat cgttttcttc cttctcccca cgtgcaatcc tgatttaaat aattgtcgct
      841 ttttgatttg gaaatgctca ctcttccatt tttttattat ttaacacaca cagaacaacc
      901 ttcacaggat cccgggatgc cgccgaaggt cgcaaagaag ggtgagaagc gtgccggcaa
      961 ggccgctgcc aaggctgcct ctggcgccga caaggacaag aagaagaaga agcgccgcaa
     1021 ggaatcgtac ggcatctaca tctacaaggt cctccgccaa gtccacgccg acaccggcat
     1081 ctccaaccgc gccatgaaca tcatgaactc gttcgtcaac gacatcttcg agcgcattgc
     1141 tggcgaggcg tcccgtctgg cccacttcaa caaccgctcg accatctcgt ctcgcgagat
     1201 ccagactgcc gtccgtctcc tcctccccgg cgaacttgcc aagcacgccg tctctgaggg
     1261 caccaaggcc gtcaccaagt acacctccgg cgccaagact agtgatatca tggtgagcaa
     1321 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     1381 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     1441 cctgaagctg atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     1501 cctgggctac ggcctgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt
     1561 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     1621 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     1681 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     1741 caactacaac agccacaacg tctatatcac cgccgacaag cagaagaacg gcatcaaggc
     1801 caacttcaag atccgccaca acatcgagga cggcggcgtg cagctcgccg accactacca
     1861 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcta
     1921 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     1981 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaaa ttttgtgttt
     2041 gccaagacac atttgtttat ttcaatcatt tctaagacca aaaagggatt tcatgatttt
     2101 tgttgcttgc attgaagttt tttttggttg tcattttttt tgcgttggtt atgagcgcaa
     2161 tatattccat tatggcaaga aacacgacga gttgttttta cagtggaggg atgagcaagg
     2221 aatggggaaa tggagaacaa gtggaatgaa atggtttgac tttgtgcggc gaaagtgttg
     2281 ccattttcaa tcatgctgcg cctggcacct agatgcgtgt gattgctcat tgctagtgct
     2341 gttctcacc
//
