LOCUS       sequence-210385-        1113 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid expresses Venus fluorescent protein fused to endogenous
            Histone H2B (H2B) gene of Capsaspora (CAOG_01818). It can be used to
            transfect Capsaspora cells and visualize nucleus in vivo..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Parra-Acero H, Ros-Rocher N, Perez-Posada A, Kozyczkowska A, 
            Sanchez-Pons N, Nakata A, Suga H, Najle SR, Ruiz-Trillo I
  TITLE     Transfection of Capsaspora owczarzaki, a close unicellular relative 
            of animals.
  JOURNAL   Development. 2018 May 11. pii: dev.162107. doi: 10.1242/dev.162107.
  PUBMED    29752387
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development. 2018 May 11. pii: dev.162107. doi: 
            10.1242/dev.162107."
COMMENT     Sequence Label: pONSY-CoH2B:Venus
FEATURES             Location/Qualifiers
     source          1..1113
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(394..396,397..399,400..1113)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(439..460)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(700..719)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1047..1068
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atgccgccga aggtcgcaaa gaagggtgag aagcgtgccg gcaaggccgc tgccaaggct
       61 gcctctggcg ccgacaagga caagaagaag aagaagcgcc gcaaggaatc gtacggcatc
      121 tacatctaca aggtcctccg ccaagtccac gccgacaccg gcatctccaa ccgcgccatg
      181 aacatcatga actcgttcgt caacgacatc ttcgagcgca ttgctggcga ggcgtcccgt
      241 ctggcccact tcaacaaccg ctcgaccatc tcgtctcgcg agatccagac tgccgtccgt
      301 ctcctcctcc ccggcgaact tgccaagcac gccgtctctg agggcaccaa ggccgtcacc
      361 aagtacacct ccggcgccaa gactagtgat atcatggtga gcaagggcga ggagctgttc
      421 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
      481 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gctgatctgc
      541 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctggg ctacggcctg
      601 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
      661 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
      721 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
      781 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
      841 aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc
      901 cacaacatcg aggacggcgg cgtgcagctc gccgaccact accagcagaa cacccccatc
      961 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc cgccctgagc
     1021 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     1081 atcactctcg gcatggacga gctgtacaag taa
//
