LOCUS       sequence-210387-        2028 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid expresses mCherry fluorescent protein fused to an 
            N-Myristoylation motif (NMM) from the endogenous Src2 gene 
            (CAOG_06360). It can be used to visualize the membrane and 
            filopodia..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Parra-Acero H, Ros-Rocher N, Perez-Posada A, Kozyczkowska A, 
            Sanchez-Pons N, Nakata A, Suga H, Najle SR, Ruiz-Trillo I
  TITLE     Transfection of Capsaspora owczarzaki, a close unicellular relative 
            of animals.
  JOURNAL   Development. 2018 May 11. pii: dev.162107. doi: 10.1242/dev.162107.
  PUBMED    29752387
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development. 2018 May 11. pii: dev.162107. doi: 
            10.1242/dev.162107."
COMMENT     Sequence Label: pONSY-CoNMM:mCherry
FEATURES             Location/Qualifiers
     source          1..2028
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             992..1702
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1137..1155)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1405..1424
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1599..1622
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 aaatgcacag ttagcaacga ccgacaatca gcaacctcca aactctcagt ttttttcccc
       61 caatcgttgt cgatttagct tttgatgctg cattttgaaa tagaacgaca accgaatttg
      121 ggagagagca ctcttttaca ggtttgtgaa aagaaaaaaa atcgaacgtg acttgcttcc
      181 atcttccttc tcgaagccga tctcggccca tccccgcttg tcccctcctc ccctcctcgc
      241 aatccgctag acagtgtctc tgcacactgg attgcggtgc gcgggccatc cgatgcccct
      301 ttcctcggcc tggagatgga cgtgaaagtg gaaaacgaaa aacgcgaaac agaaacatgt
      361 ggttcttttt tttttctttt tctttttcgc cttcgggctt ttctctctcg cttcctcttc
      421 tccgctctct tcttcgcgct cttttgcctc ttgcgtgaga tgctcgtctt gcaatcgtct
      481 gccactgctg cgctgtcacg atcgatcgca tcggacaagc cgtttctgtt ttgaaattta
      541 gaacgttctg gaaaacaaca aaaaattgcc tttttttcac ttttttcttt tttcgttttt
      601 ttccctcttc aacacttcct ccaactgtcg ttctgcgttt gttcttcgct ctcgtcgtgc
      661 acactcggat taggatccat tcatccccat ccccttgcgc attgccaaaa acgagcgtcg
      721 aaacgcgaac gtcagcgtgg ggtgttgacg ccgaacccca cgagtgtttt tttttaaaat
      781 gctgtttcat cgttttcttc cttctcccca cgtgcaatcc tgatttaaat aattgtcgct
      841 ttttgatttg gaaatgctca ctcttccatt tttttattat ttaacacaca cagaacaacc
      901 ttcacaggat cccgggatgg gctgctccaa ctctaaaccg cacgacccgt ccgatttcaa
      961 ggtttcccct tctggcgttg cgtccaacag catggtgagc aagggcgagg aggacaacat
     1021 ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca
     1081 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
     1141 gctgaaggtg accaagggcg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt
     1201 catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct
     1261 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt
     1321 gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg
     1381 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
     1441 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct
     1501 gaagctgaag gacggcggcc actacgacgc cgaggtcaag accacctaca aggccaagaa
     1561 gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ctggacatca cctcccacaa
     1621 cgaggactac accatcgtgg aacagtacga gcgcgccgag ggccgccact ccaccggcgg
     1681 catggacgag ctgtacaagt aaatctgaat tttgtgtttg ccaagacaca tttgtttatt
     1741 tcaatcattt ctaagaccaa aaagggattt catgattttt gttgcttgca ttgaagtttt
     1801 ttttggttgt catttttttt gcgttggtta tgagcgcaat atattccatt atggcaagaa
     1861 acacgacgag ttgtttttac agtggaggga tgagcaagga atggggaaat ggagaacaag
     1921 tggaatgaaa tggtttgact ttgtgcggcg aaagtgttgc cattttcaat catgctgcgc
     1981 ctggcaccta gatgcgtgtg attgctcatt gctagtgctg ttctcacc
//
