LOCUS       sequence-210388-         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid expresses mCherry fluorescent protein fused to an 
            N-Myristoylation motif (NMM) from the endogenous Src2 gene 
            (CAOG_06360). It can be used to visualize the membrane and 
            filopodia..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Parra-Acero H, Ros-Rocher N, Perez-Posada A, Kozyczkowska A, 
            Sanchez-Pons N, Nakata A, Suga H, Najle SR, Ruiz-Trillo I
  TITLE     Transfection of Capsaspora owczarzaki, a close unicellular relative 
            of animals.
  JOURNAL   Development. 2018 May 11. pii: dev.162107. doi: 10.1242/dev.162107.
  PUBMED    29752387
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development. 2018 May 11. pii: dev.162107. doi: 
            10.1242/dev.162107."
COMMENT     Sequence Label: pONSY-CoNMM:mCherry
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             76..786
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(221..239)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     489..508
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     683..706
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 atgggctgct ccaactctaa accgcacgac ccgtccgatt tcaaggtttc cccttctggc
       61 gttgcgtcca acagcatggt gagcaagggc gaggaggaca acatggccat catcaaggag
      121 ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag
      181 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag
      241 ggcggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag
      301 gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc
      361 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac
      421 tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc
      481 tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg
      541 taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc
      601 ggccactacg acgccgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc
      661 ggcgcctaca acgtcaacat caagctggac atcacctccc acaacgagga ctacaccatc
      721 gtggaacagt acgagcgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac
      781 aagtaa
//
