LOCUS       sequence-210407-        5215 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-terminal GFP tag on Hook3 (amino acids 1-522) for expression in 
            Sf9 cells. C-terminal GFP-Psc-StrepII tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5215)
  AUTHORS   Urnavicius L, Lau CK, Elshenawy MM, Morales-Rios E, Motz C, Yildiz 
            A, Carter AP
  TITLE     Cryo-EM shows how dynactin recruits two dyneins for faster movement.
  JOURNAL   Nature. 2018 Feb 7;554(7691):202-206. doi: 10.1038/nature25462.
  PUBMED    29420470
REFERENCE   2  (bases 1 to 5215)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2018-02-7- 7"; volume: "554"; issue: "7691"; pages:
            "202-206"
COMMENT     Sequence Label: Hook3(1-522)-GFP-Psc-StrepII
FEATURES             Location/Qualifiers
     source          1..5215
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(66..99)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        176..267
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     192..209
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             2570..2593
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             2594..2617
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             2654..2677
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     polyA_signal    2837..2971
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2874..2893)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2928..2947
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  3092..3257
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      3447..4035
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3936..3955
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  4170..4393
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4460..4993)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(5182..5187,5188..5204,5205..5210))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 ttcgtgcctt catccgtttc cacggtgtgc gtcacccggc aaccttgggc agcagcgaag
       61 tcgccataac ttcgtatagc atacattata cgaagttatc tgtaactata acggtcctaa
      121 ggtagcgagt ttaaacacta gtatcgattc gcgacctact ccggaatatt aatagatcat
      181 ggagataatt aaaatgataa ccatctcgca aataaataag tattttactg ttttcgtaac
      241 agttttgtaa taaaaaaacc tataaatatt ccggattatt cataccgtcc caccatcggg
      301 cgcggatccc tcgagcatgt tctctgttga atcattggag agggcagaat tgtgtgagtc
      361 cctgctgacc tggattcaga cattcaacgt ggacgctccc tgccagactg tcgaggatct
      421 gacaaatggc gtggttatgg ctcaggtcct ccagaagatc gacccagcat acttcgatga
      481 gaactggctc aacaggatca agaccgaagt cggagacaat tggaggctca agatttcaaa
      541 cctgaagaag attctcaagg gtatcctcga ctataaccat gaaatcttgg gtcaacaaat
      601 caacgatttc actctccctg acgtcaatct cattggtgag cactctgatg ctgctgagct
      661 gggcaggatg ctgcaactga tcctgggatg tgcagtgaac tgcgagcaga agcaggagta
      721 cattcaggca atcatgatga tggaggaatc cgtgcagcac gtggtgatga ctgctatcca
      781 ggaactcatg tccaaggaat ctcccgtctc tgcaggcaac gatgcttacg tcgatctcga
      841 tcgccagctg aagaagacaa ccgaggaact gaatgaggct ctctctgcaa aggaggagat
      901 cgctcagcgc tgccacgaac tggacatgca ggtggcagca ttgcaggagg agaagtctag
      961 cctgttggca gagaaccagg tcttgatgga gaggttgaac cagtcagatt ctatcgaaga
     1021 cccaaatagc ccagctggta gaagacacct gcaattgcaa actcaactgg agcagctcca
     1081 ggaggagaca tttcgcctgg aggcagctaa ggacgactac cgcatccgct gcgaggaatt
     1141 ggagaaagaa atcagcgaac tcaggcaaca gaacgatgag ctgactaccc tcgcagacga
     1201 ggctcaatcc ctgaaggatg agatcgatgt gttgaggcac agctcagata aagtgtccaa
     1261 gctggaaggt caggtggagt cctacaagaa gaaattggag gatctcggtg atctgcgcag
     1321 gcaggttaaa ctgctcgaag agaagaacac catgtatatg cagaatacag tctccctgga
     1381 agaggaactc agaaaggcca atgctgcaag atcacagttg gaaacttaca agagacaagt
     1441 cgtcgagttg cagaataggc tgtctgagga aagcaagaaa gctgacaagc tggactttga
     1501 atacaagaga ttgaaagaga aggttgactc cctgcagaag gagaaagata ggttgaggac
     1561 tgagagggac tcactgaaag agacaatcga ggagctgaga tgcgtccagg ctcaagaagg
     1621 ccagctgacc acacagggac tcatgcctct gggctctcag gagtctagcg attcactggc
     1681 agccgagatc gttactccag aaatcagaga gaagctcatt cgcctgcagc acgagaacaa
     1741 gatgctcaaa ctcaaccaag agggttcaga caacgagaag atcgcactgt tgcagtcttt
     1801 gctggacgac gcaaacttga ggaagaatga actcgaaact gagaacaggc tggtgaatca
     1861 gaggttgctg gaagtgcaat catctaaagg tgaagaatta ttcactggtg ttgtcccaat
     1921 tttggttgaa ttagatggtg atgttaatgg tcacaaattt tctgtctccg gtgaaggtga
     1981 aggtgatgct acttacggta aattgacctt aaaatttatt tgtactactg gtaaattgcc
     2041 agttccatgg ccaaccttag tcactacttt cggttatggt gttcaatgtt ttgcgagata
     2101 cccagatcat atgaaacaac atgacttttt caagtctgcc atgccagaag gttatgttca
     2161 agaaagaact atttttttca aagatgacgg taactacaag accagagctg aagtcaagtt
     2221 tgaaggtgat accttagtta atagaatcga attaaaaggt attgatttta aagaagatgg
     2281 taacatttta ggtcacaaat tggaatacaa ctataactct cacaatgttt acatcatggc
     2341 tgacaaacaa aagaatggta tcaaagttaa cttcaaaatt agacacaaca ttgaagacgg
     2401 ttctgttcaa ttagctgacc attatcaaca aaatactcca attggtgatg gtccagtctt
     2461 gttaccagac aaccattact tatccactca atctgcctta tccaaggatc caaacgaaaa
     2521 gagagaccac atggtcttgt tagaatttgt tactgctgct ggtattaccc tggaagttct
     2581 gtttcagggt ccgtggtcac atccgcagtt tgaaaaaggt agcgcaggta gtgcagcagg
     2641 tagcggtgca ggttggagcc atcctcagtt tgagaaataa ggtacctcta gagcctgcag
     2701 tctcgacaag cttgtcgaga agtactagag gatcataatc agccatacca catttgtaga
     2761 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     2821 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     2881 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     2941 actcatcaat gtatcttatc atgtctggat ctgatcactg cttgagccta gaagatccgg
     3001 ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactat
     3061 cataacccct agggtatacc catctaattg gaaccagata agtgaaatct agttccaaac
     3121 tattttgtca tttttaattt tcgtattagc ttacgacgct acacccagtt cccatctatt
     3181 ttgtcactct tccctaaata atccttaaaa actccatttc cacccctccc agttcccaac
     3241 tattttgtcc gcccacaacc ggttgacttg ggtcaactgt cagaccaagt ttactcatat
     3301 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     3361 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     3421 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     3481 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     3541 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     3601 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     3661 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     3721 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     3781 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     3841 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     3901 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     3961 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     4021 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     4081 ccttttgctc acatgttctt tcctgcgtta tcccctgatt gacttgggtc gctcttcctg
     4141 tggatgcgca gatgccctgc gtaagcgggt gtgggcggac aataaagtct taaactgaac
     4201 aaaatagatc taaactatga caataaagtc ttaaactaga cagaatagtt gtaaactgaa
     4261 atcagtccag ttatgctgtg aaaaagcata ctggactttt gttatggcta aagcaaactc
     4321 ttcattttct gaagtgcaaa ttgcccgtcg tattaaagag gggcgtggcc aagggcatgt
     4381 aaagactata ttcgcggcgt tgtgacaatt taccgaacaa ctccgcggcc gggaagccga
     4441 tctcggcttg aacgaattgt taggtggcgg tacttgggtc gatatcaaag tgcatcactt
     4501 cttcccgtat gcccaacttt gtatagagag ccactgcggg atcgtcaccg taatctgctt
     4561 gcacgtagat cacataagca ccaagcgcgt tggcctcatg cttgaggaga ttgatgagcg
     4621 cggtggcaat gccctgcctc cggtgctcgc cggagactgc gagatcatag atatagatct
     4681 cactacgcgg ctgctcaaac ttgggcagaa cgtaagccgc gagagcgcca acaaccgctt
     4741 cttggtcgaa ggcagcaagc gcgatgaatg tcttactacg gagcaagttc ccgaggtaat
     4801 cggagtccgg ctgatgttgg gagtaggtgg ctacgtctcc gaactcacga ccgaaaagat
     4861 caagagcagc ccgcatggat ttgacttggt cagggccgag cctacatgtg cgaatgatgc
     4921 ccatacttga gccacctaac tttgttttag ggcgactgcc ctgctgcgta acatcgttgc
     4981 tgctgcgtaa catcgttgct gctccataac atcaaacatc gacccacggc gtaacgcgct
     5041 tgctgcttgg atgcccgagg catagactgt acaaaaaaac agtcataaca agccatgaaa
     5101 accgccactg cgccgttacc accgctgcgt tcggtcaagg ttctggacca gttgcgtgag
     5161 cgcatacgct acttgcatta cagtttacga accgaacagg cttatgtcaa ctggg
//
