LOCUS       sequence-210411-        5071 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-terminal SNAPf tag on Hook3 (amino acids 1-522) for expression in 
            Sf9 cells. C-terminal SNAPf-Psc-StrepII tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5071)
  AUTHORS   Urnavicius L, Lau CK, Elshenawy MM, Morales-Rios E, Motz C, Yildiz 
            A, Carter AP
  TITLE     Cryo-EM shows how dynactin recruits two dyneins for faster movement.
  JOURNAL   Nature. 2018 Feb 7;554(7691):202-206. doi: 10.1038/nature25462.
  PUBMED    29420470
REFERENCE   2  (bases 1 to 5071)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2018-02-7- 7"; volume: "554"; issue: "7691"; pages:
            "202-206"
COMMENT     Sequence Label: Hook3(1-522)-SNAPf-Psc-StrepII
FEATURES             Location/Qualifiers
     source          1..5071
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(66..99)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        176..267
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     192..209
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             1883..2425
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="DKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVEV
                     PAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLKV
                     VKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLAV
                     KEWLLAHEGHRLGKPGLG"
     CDS             2426..2449
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             2450..2473
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             2510..2533
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     polyA_signal    2693..2827
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2730..2749)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2784..2803
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  2948..3113
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      3303..3891
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3792..3811
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  4026..4249
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4316..4849)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(5038..5043,5044..5060,5061..5066))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 ttcgtgcctt catccgtttc cacggtgtgc gtcacccggc aaccttgggc agcagcgaag
       61 tcgccataac ttcgtatagc atacattata cgaagttatc tgtaactata acggtcctaa
      121 ggtagcgagt ttaaacacta gtatcgattc gcgacctact ccggaatatt aatagatcat
      181 ggagataatt aaaatgataa ccatctcgca aataaataag tattttactg ttttcgtaac
      241 agttttgtaa taaaaaaacc tataaatatt ccggattatt cataccgtcc caccatcggg
      301 cgcggatccc tcgagcatgt tctctgttga atcattggag agggcagaat tgtgtgagtc
      361 cctgctgacc tggattcaga cattcaacgt ggacgctccc tgccagactg tcgaggatct
      421 gacaaatggc gtggttatgg ctcaggtcct ccagaagatc gacccagcat acttcgatga
      481 gaactggctc aacaggatca agaccgaagt cggagacaat tggaggctca agatttcaaa
      541 cctgaagaag attctcaagg gtatcctcga ctataaccat gaaatcttgg gtcaacaaat
      601 caacgatttc actctccctg acgtcaatct cattggtgag cactctgatg ctgctgagct
      661 gggcaggatg ctgcaactga tcctgggatg tgcagtgaac tgcgagcaga agcaggagta
      721 cattcaggca atcatgatga tggaggaatc cgtgcagcac gtggtgatga ctgctatcca
      781 ggaactcatg tccaaggaat ctcccgtctc tgcaggcaac gatgcttacg tcgatctcga
      841 tcgccagctg aagaagacaa ccgaggaact gaatgaggct ctctctgcaa aggaggagat
      901 cgctcagcgc tgccacgaac tggacatgca ggtggcagca ttgcaggagg agaagtctag
      961 cctgttggca gagaaccagg tcttgatgga gaggttgaac cagtcagatt ctatcgaaga
     1021 cccaaatagc ccagctggta gaagacacct gcaattgcaa actcaactgg agcagctcca
     1081 ggaggagaca tttcgcctgg aggcagctaa ggacgactac cgcatccgct gcgaggaatt
     1141 ggagaaagaa atcagcgaac tcaggcaaca gaacgatgag ctgactaccc tcgcagacga
     1201 ggctcaatcc ctgaaggatg agatcgatgt gttgaggcac agctcagata aagtgtccaa
     1261 gctggaaggt caggtggagt cctacaagaa gaaattggag gatctcggtg atctgcgcag
     1321 gcaggttaaa ctgctcgaag agaagaacac catgtatatg cagaatacag tctccctgga
     1381 agaggaactc agaaaggcca atgctgcaag atcacagttg gaaacttaca agagacaagt
     1441 cgtcgagttg cagaataggc tgtctgagga aagcaagaaa gctgacaagc tggactttga
     1501 atacaagaga ttgaaagaga aggttgactc cctgcagaag gagaaagata ggttgaggac
     1561 tgagagggac tcactgaaag agacaatcga ggagctgaga tgcgtccagg ctcaagaagg
     1621 ccagctgacc acacagggac tcatgcctct gggctctcag gagtctagcg attcactggc
     1681 agccgagatc gttactccag aaatcagaga gaagctcatt cgcctgcagc acgagaacaa
     1741 gatgctcaaa ctcaaccaag agggttcaga caacgagaag atcgcactgt tgcagtcttt
     1801 gctggacgac gcaaacttga ggaagaatga actcgaaact gagaacaggc tggtgaatca
     1861 gaggttgctg gaagtgcaat cagacaaaga ctgcgaaatg aagcgcacca ccctggatag
     1921 ccctctgggc aagctggaac tgtctgggtg cgaacagggc ctgcaccgta tcatcttcct
     1981 gggcaaagga acatctgccg ccgacgccgt ggaagtgcct gccccagccg ccgtgctggg
     2041 cggaccagag ccactgatgc aggccaccgc ctggctcaac gcctactttc accagcctga
     2101 ggccatcgag gagttccctg tgccagccct gcaccaccca gtgttccagc aggagagctt
     2161 tacccgccag gtgctgtgga aactgctgaa agtggtgaag ttcggagagg tcatcagcta
     2221 cagccacctg gccgccctgg ccggcaatcc cgccgccacc gccgccgtga aaaccgccct
     2281 gagcggaaat cccgtgccca ttctgatccc ctgccaccgg gtggtgcagg gcgacctgga
     2341 cgtggggggc tacgagggcg ggctcgccgt gaaagagtgg ctgctggccc acgagggcca
     2401 cagactgggc aagcctgggc tgggtctgga agttctgttt cagggtccgt ggtcacatcc
     2461 gcagtttgaa aaaggtagcg caggtagtgc agcaggtagc ggtgcaggtt ggagccatcc
     2521 tcagtttgag aaataaggta cctctagagc ctgcagtctc gacaagcttg tcgagaagta
     2581 ctagaggatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
     2641 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
     2701 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     2761 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     2821 ctggatctga tcactgcttg agcctagaag atccggctgc taacaaagcc cgaaaggaag
     2881 ctgagttggc tgctgccacc gctgagcaat aactatcata acccctaggg tatacccatc
     2941 taattggaac cagataagtg aaatctagtt ccaaactatt ttgtcatttt taattttcgt
     3001 attagcttac gacgctacac ccagttccca tctattttgt cactcttccc taaataatcc
     3061 ttaaaaactc catttccacc cctcccagtt cccaactatt ttgtccgccc acaaccggtt
     3121 gacttgggtc aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     3181 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     3241 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     3301 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     3361 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     3421 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     3481 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     3541 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     3601 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     3661 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     3721 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     3781 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3841 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     3901 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     3961 gcgttatccc ctgattgact tgggtcgctc ttcctgtgga tgcgcagatg ccctgcgtaa
     4021 gcgggtgtgg gcggacaata aagtcttaaa ctgaacaaaa tagatctaaa ctatgacaat
     4081 aaagtcttaa actagacaga atagttgtaa actgaaatca gtccagttat gctgtgaaaa
     4141 agcatactgg acttttgtta tggctaaagc aaactcttca ttttctgaag tgcaaattgc
     4201 ccgtcgtatt aaagaggggc gtggccaagg gcatgtaaag actatattcg cggcgttgtg
     4261 acaatttacc gaacaactcc gcggccggga agccgatctc ggcttgaacg aattgttagg
     4321 tggcggtact tgggtcgata tcaaagtgca tcacttcttc ccgtatgccc aactttgtat
     4381 agagagccac tgcgggatcg tcaccgtaat ctgcttgcac gtagatcaca taagcaccaa
     4441 gcgcgttggc ctcatgcttg aggagattga tgagcgcggt ggcaatgccc tgcctccggt
     4501 gctcgccgga gactgcgaga tcatagatat agatctcact acgcggctgc tcaaacttgg
     4561 gcagaacgta agccgcgaga gcgccaacaa ccgcttcttg gtcgaaggca gcaagcgcga
     4621 tgaatgtctt actacggagc aagttcccga ggtaatcgga gtccggctga tgttgggagt
     4681 aggtggctac gtctccgaac tcacgaccga aaagatcaag agcagcccgc atggatttga
     4741 cttggtcagg gccgagccta catgtgcgaa tgatgcccat acttgagcca cctaactttg
     4801 ttttagggcg actgccctgc tgcgtaacat cgttgctgct gcgtaacatc gttgctgctc
     4861 cataacatca aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata
     4921 gactgtacaa aaaaacagtc ataacaagcc atgaaaaccg ccactgcgcc gttaccaccg
     4981 ctgcgttcgg tcaaggttct ggaccagttg cgtgagcgca tacgctactt gcattacagt
     5041 ttacgaaccg aacaggctta tgtcaactgg g
//
