LOCUS       sequence-210415-        6792 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Full length BICDR1 for expression in Sf9 cells. 8xHis-ZZ N-terminal 
            tag, TEV site to cleave 8x-His-ZZ..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6792)
  AUTHORS   Urnavicius L, Lau CK, Elshenawy MM, Morales-Rios E, Motz C, Yildiz 
            A, Carter AP
  TITLE     Cryo-EM shows how dynactin recruits two dyneins for faster movement.
  JOURNAL   Nature. 2018 Feb 7;554(7691):202-206. doi: 10.1038/nature25462.
  PUBMED    29420470
REFERENCE   2  (bases 1 to 6792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2018-02-7- 7"; volume: "554"; issue: "7691"; pages:
            "202-206"
COMMENT     Sequence Label: His-ZZ-TEV-BICDR1
FEATURES             Location/Qualifiers
     source          1..6792
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(92..625)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(814..819,820..836,837..842))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(913..946)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        1023..1114
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     1039..1056
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             1181..1204
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             1247..1420
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             1421..1594
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             1652..1672
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1694..1714
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    3576..3710
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3613..3632)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3667..3686
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  3831..3996
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     misc_recomb     4003..4708
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     3'UTR           4091..4463
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     CDS             complement(4120..4708)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR
                     PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR
                     LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF
                     ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     rep_origin      4898..5486
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5387..5406
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_recomb     5738..6565
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             5738..5862
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(5900..6505)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     mobile_element  join(6594..6792,1..25)
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
ORIGIN
        1 ccaagggcat gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg
       61 ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa
      121 agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac
      181 cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga
      241 gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat
      301 agatatagat ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc
      361 caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt
      421 tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac
      481 gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg
      541 tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg
      601 taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg
      661 gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa
      721 caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac
      781 cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc
      841 aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc
      901 agcgaagtcg ccataacttc gtatagcata cattatacga agttatctgt aactataacg
      961 gtcctaaggt agcgagttta aacactagta tcgattcgcg acctactccg gaatattaat
     1021 agatcatgga gataattaaa atgataacca tctcgcaaat aaataagtat tttactgttt
     1081 tcgtaacagt tttgtaataa aaaaacctat aaatattccg gattattcat accgtcccac
     1141 catcgggcgc ggatctcggt ccgaaaccat gtcgtactac catcaccatc accatcacca
     1201 tcacggttct atgtgcatgg caggccttgc gcaacacgat gaagccgtgg acaacaaatt
     1261 caacaaagaa caacaaaacg cgttctatga gatcttacat ttacctaact taaacgaaga
     1321 acaacgaaac gccttcatcc aaagtttaaa agatgaccca agccaaagcg ctaacctttt
     1381 agcagaagct aaaaagctaa atgatgctca ggcgccgaaa gtagacaaca aattcaacaa
     1441 agaacaacaa aacgcgttct atgagatctt acatttacct aacttaaacg aagaacaacg
     1501 aaacgccttc atccaaagtt taaaagatga cccaagccaa agcgctaacc ttttagcaga
     1561 agctaaaaag ctaaatgatg ctcaggcgcc gaaagtagac gcaaattccg cggggaagtc
     1621 aaccgattat gatattccaa ctactgctag cgagaaccta tatttccagg gagattatga
     1681 tatccctacg actgagaatc tttattttca gggctccgcc ttctgcctgg gcttggccgg
     1741 ccgagcttca gcacccgccg agccggacag cgcctgctgc atggagctgc ccgctggggc
     1801 cggggacgca gtccggagtc ccgccactgc tgccgccctc gtctccttcc ccggaggtcc
     1861 cggggaactg gaactggcct tagaggagga gctggcgttg ctggcggccg gggagcggtc
     1921 gtccgagccc ggcgagcatc ctcaggcgga gccggagtct ccggttgagg ggcacgggcc
     1981 gccgctgccg ccgccgccta cccaggatcc cgagctgctg tcggtgatcc gccagaagga
     2041 gaaggatctg gtgttggcgg cccgactagg caaggcgctg ctcgagagga accaggacat
     2101 gagccggcag tacgagcaga tgcacaagga gctgacggac aagctagagc acctagaaca
     2161 agagaaacat gaactgagaa ggcgattcga gaatcgagaa ggggagtggg aaggacgggt
     2221 gtctgagctg gagacggacg tgaagcagct acaggatgag ttggagaggc agcagctcca
     2281 ccttcgggaa gcagaccgag aaaaaacacg agctgtccag gaactgtcag aacagaacca
     2341 gaggctgctg gaccaactca gcagggcatc tgaagttgag agacagctct ccatgcaggt
     2401 ccatgctctc aaagaagact tccgggagaa aaattcctcc accaaccagc atatcatccg
     2461 gctggagagt ctgcaggccg agatcaagat gctgtcagat cgaaaacggg agctggagca
     2521 tcggctcagc gccaccttgg aggagaatga cctgcttcaa gggactgtgg aggagctgca
     2581 ggaccgggtg ctgatcctag aaaggcaggg ccatgacaaa gacctacagc tgcaccaaag
     2641 ccagctggag ctccaggagg tgcggctgtc ctaccggcag ctgcagggca aggtggagga
     2701 actcacggag gagaggagtt tgcagagctc ggctgcgacc agcacgtccc tgctgtcaga
     2761 gatcgagcag agcatggagg ccgaggagct ggagcaggag agagagcagc tgaggctgca
     2821 gctctgggaa gcctactgcc aggttcgata tctctgctca cacctccgag ggaatgacag
     2881 tgccgactca gccgtatcca cagactcctc catggacgag tcctcggaga cctcgtcggc
     2941 caaggacgtg cctgccggca gcctgcgcac tgccctcaac gatctcaaga ggctgataca
     3001 gagcatcgtg gacggtgtgg agcccacggt gacactgctg agcgtggaga tgaccgcact
     3061 gaaggaggaa agggaccgac tcagagtgac ctctgaggac aaggagccaa aggagcagct
     3121 tcagaaggcc atcagggacc gggacgaggc cattgccaag aagaacgctg tggagctgga
     3181 actcgccaag tgcaagatgg atatgatgtc tctgaacagc cagctactgg atgccattca
     3241 gcaaaaactg aacctctctc agcagctgga ggcttggcag gatgacatgc acagggtcat
     3301 tgacaggcag ctgatggaca cgcacctgaa ggaacagagc cggcctgctg ctgctgcctt
     3361 ccctaggggc cacggcgtag ggcgggggca ggaacccagc accgcagatg gcaaacggct
     3421 cttctccttc ttcaggaaga tttaatgagc ttgtcgagaa gtactagagg atcataatca
     3481 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     3541 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     3601 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     3661 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc tgatcactgc
     3721 ttgagcctag aagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc
     3781 accgctgagc aataactatc ataaccccta gggtataccc atctaattgg aaccagataa
     3841 gtgaaatcta gttccaaact attttgtcat ttttaatttt cgtattagct tacgacgcta
     3901 cacccagttc ccatctattt tgtcactctt ccctaaataa tccttaaaaa ctccatttcc
     3961 acccctccca gttcccaact attttgtccg cccacaaccg gtggaggaaa ttctccttga
     4021 agtttccctg gtgttcaaag taaaggagtt tgcaccagac gcacctctgt tcactggtcc
     4081 ggcgtattaa aacacgatac attgttatta gtacatttat taagcgctag attctgtgcg
     4141 ttgttgattt acagacaatt gttgtacgta ttttaataat tcattaaatt tataatcttt
     4201 agggtggtat gttagagcga aaatcaaatg attttcagcg tctttatatc tgaatttaaa
     4261 tattaaatcc tcaatagatt tgtaaaatag gtttcgatta gtttcaaaca agggttgttt
     4321 ttccgaaccg atggctggac tatctaatgg attttcgctc aacgccacaa aacttgccaa
     4381 atcttgtagc agcaatctag ctttgtcgat attcgtttgt gttttgtttt gtaataaagg
     4441 ttcgacgtcg ttcaaaatat tatgcgcttt tgtatttctt tcatcactgt cgttagtgta
     4501 caattgactc gacgtaaaca cgttaaatag agcttggaca tatttaacat cgggcgtgtt
     4561 agctttatta ggccgattat cgtcgtcgtc ccaaccctcg tcgttagaag ttgcttccga
     4621 agacgatttt gccatagcca cacgacgcct attaattgtg tcggctaaca cgtccgcgat
     4681 caaatttgta gttgagcttt ttggaattac cggttgactt gggtcaactg tcagaccaag
     4741 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     4801 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     4861 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     4921 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     4981 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     5041 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     5101 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     5161 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     5221 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     5281 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     5341 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     5401 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     5461 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     5521 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tgacttgggt
     5581 cgctcttcct gtggatgcgc aggtatgtac aggaagaggt ttatactaaa ctgttacatt
     5641 gcaaacgtgg tttcgtgtgc caagtgtgaa aaccgatgtt taatcaaggc tctgacgcat
     5701 ttctacaacc acgactctaa gtgtgtgggt gaagtcatgc atcttttaat caaatcccaa
     5761 gatgtgtata aaccaccaaa ctgccaaaaa atgaaaactg tcgacaagct ctgtccgttt
     5821 gctggcaact gcaagggtct caatcctatt tgtaattatt gaataataaa acaattataa
     5881 atgtcaaatt tgttttttat taacgataca aaccaaacgc aacaagaaca tttgtagtat
     5941 tatctataat tgaaaacgcg tagttataat cgctgaggta atatttaaaa tcattttcaa
     6001 atgattcaca gttaatttgc gacaatataa ttttattttc acataaacta gacgccttgt
     6061 cgtcttcttc ttcgtattcc ttctcttttt catttttctc ttcataaaaa ttaacatagt
     6121 tattatcgta tccatatatg tatctatcgt atagagtaaa ttttttgttg tcataaatat
     6181 atatgtcttt tttaatgggg tgtatagtac cgctgcgcat agtttttctg taatttacaa
     6241 cagtgctatt ttctggtagt tcttcggagt gtgttgcttt aattattaaa tttatataat
     6301 caatgaattt gggatcgtcg gttttgtaca atatgttgcc ggcatagtac gcagcttctt
     6361 ctagttcaat tacaccattt tttagcagca ccggattaac ataactttcc aaaatgttgt
     6421 acgaaccgtt aaacaaaaac agttcacctc ccttttctat actattgtct gcgagcagtt
     6481 gtttgttgtt aaaaataaca gccattgtaa tgagacgcac aaactaatat cacaaactgg
     6541 aaatgtctat caatatatag ttgctgattg cgcagatgcc ctgcgtaagc gggtgtgggc
     6601 ggacaataaa gtcttaaact gaacaaaata gatctaaact atgacaataa agtcttaaac
     6661 tagacagaat agttgtaaac tgaaatcagt ccagttatgc tgtgaaaaag catactggac
     6721 ttttgttatg gctaaagcaa actcttcatt ttctgaagtg caaattgccc gtcgtattaa
     6781 agaggggcgt gg
//
