LOCUS       sequence-210418-        7485 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-terminal GFP tag on BICDR1 for expression in Sf9 cells. 
            8xHis-ZZ-GFP N-terminal tag, TEV site to cleave 8xHis-ZZ..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7485)
  AUTHORS   Urnavicius L, Lau CK, Elshenawy MM, Morales-Rios E, Motz C, Yildiz 
            A, Carter AP
  TITLE     Cryo-EM shows how dynactin recruits two dyneins for faster movement.
  JOURNAL   Nature. 2018 Feb 7;554(7691):202-206. doi: 10.1038/nature25462.
  PUBMED    29420470
REFERENCE   2  (bases 1 to 7485)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2018-02-7- 7"; volume: "554"; issue: "7691"; pages:
            "202-206"
COMMENT     Sequence Label: His-ZZ-TEV-BICDR1-GFP
FEATURES             Location/Qualifiers
     source          1..7485
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        74..165
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     90..107
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             232..255
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             298..471
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             472..645
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             703..723
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             745..765
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    3320..3454
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3357..3376)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3411..3430
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  3575..3740
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     misc_recomb     3747..4452
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     3'UTR           3835..4207
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     CDS             complement(3864..4452)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR
                     PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR
                     LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF
                     ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     rep_origin      4642..5230
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5131..5150
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_recomb     5482..6309
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             5482..5606
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(5644..6249)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     mobile_element  6338..6561
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(6628..7161)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(7350..7355,7356..7372,7373..7378))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(7449..7482)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 taactataac ggtcctaagg tagcgagttt aaacactagt atcgattcgc gacctactcc
       61 ggaatattaa tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta
      121 ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca
      181 taccgtccca ccatcgggcg cggatctcgg tccgaaacca tgtcgtacta ccatcaccat
      241 caccatcacc atcacggttc tatgtgcatg gcaggccttg cgcaacacga tgaagccgtg
      301 gacaacaaat tcaacaaaga acaacaaaac gcgttctatg agatcttaca tttacctaac
      361 ttaaacgaag aacaacgaaa cgccttcatc caaagtttaa aagatgaccc aagccaaagc
      421 gctaaccttt tagcagaagc taaaaagcta aatgatgctc aggcgccgaa agtagacaac
      481 aaattcaaca aagaacaaca aaacgcgttc tatgagatct tacatttacc taacttaaac
      541 gaagaacaac gaaacgcctt catccaaagt ttaaaagatg acccaagcca aagcgctaac
      601 cttttagcag aagctaaaaa gctaaatgat gctcaggcgc cgaaagtaga cgcaaattcc
      661 gcggggaagt caaccgatta tgatattcca actactgcta gcgagaacct atatttccag
      721 ggagattatg atatccctac gactgagaat ctttattttc agggcatgtc cgccttctgc
      781 ctgggcttgg ccggccgagc ttcagcaccc gccgagccgg acagcgcctg ctgcatggag
      841 ctgcccgctg gggccgggga cgcagtccgg agtcccgcca ctgctgccgc cctcgtctcc
      901 ttccccggag gtcccgggga actggaactg gccttagagg aggagctggc gttgctggcg
      961 gccggggagc ggtcgtccga gcccggcgag catcctcagg cggagccgga gtctccggtt
     1021 gaggggcacg ggccgccgct gccgccgccg cctacccagg atcccgagct gctgtcggtg
     1081 atccgccaga aggagaagga tctggtgttg gcggcccgac taggcaaggc gctgctcgag
     1141 aggaaccagg acatgagccg gcagtacgag cagatgcaca aggagctgac ggacaagcta
     1201 gagcacctag aacaagagaa acatgaactg agaaggcgat tcgagaatcg agaaggggag
     1261 tgggaaggac gggtgtctga gctggagacg gacgtgaagc agctacagga tgagttggag
     1321 aggcagcagc tccaccttcg ggaagcagac cgagaaaaaa cacgagctgt ccaggaactg
     1381 tcagaacaga accagaggct gctggaccaa ctcagcaggg catctgaagt tgagagacag
     1441 ctctccatgc aggtccatgc tctcaaagaa gacttccggg agaaaaattc ctccaccaac
     1501 cagcatatca tccggctgga gagtctgcag gccgagatca agatgctgtc agatcgaaaa
     1561 cgggagctgg agcatcggct cagcgccacc ttggaggaga atgacctgct tcaagggact
     1621 gtggaggagc tgcaggaccg ggtgctgatc ctagaaaggc agggccatga caaagaccta
     1681 cagctgcacc aaagccagct ggagctccag gaggtgcggc tgtcctaccg gcagctgcag
     1741 ggcaaggtgg aggaactcac ggaggagagg agtttgcaga gctcggctgc gaccagcacg
     1801 tccctgctgt cagagatcga gcagagcatg gaggccgagg agctggagca ggagagagag
     1861 cagctgaggc tgcagctctg ggaagcctac tgccaggttc gatatctctg ctcacacctc
     1921 cgagggaatg acagtgccga ctcagccgta tccacagact cctccatgga cgagtcctcg
     1981 gagacctcgt cggccaagga cgtgcctgcc ggcagcctgc gcactgccct caacgatctc
     2041 aagaggctga tacagagcat cgtggacggt gtggagccca cggtgacact gctgagcgtg
     2101 gagatgaccg cactgaagga ggaaagggac cgactcagag tgacctctga ggacaaggag
     2161 ccaaaggagc agcttcagaa ggccatcagg gaccgggacg aggccattgc caagaagaac
     2221 gctgtggagc tggaactcgc caagtgcaag atggatatga tgtctctgaa cagccagcta
     2281 ctggatgcca ttcagcaaaa actgaacctc tctcagcagc tggaggcttg gcaggatgac
     2341 atgcacaggg tcattgacag gcagctgatg gacacgcacc tgaaggaaca gagccggcct
     2401 gctgctgctg ccttccctag gggccacggc gtagggcggg ggcaggaacc cagcaccgca
     2461 gatggcaaac ggctcttctc cttcttcagg aagattatgt ctaaaggtga agaattattc
     2521 actggtgttg tcccaatttt ggttgaatta gatggtgatg ttaatggtca caaattttct
     2581 gtctccggtg aaggtgaagg tgatgctact tacggtaaat tgaccttaaa atttatttgt
     2641 actactggta aattgccagt tccatggcca accttagtca ctactttcgg ttatggtgtt
     2701 caatgttttg cgagataccc agatcatatg aaacaacatg actttttcaa gtctgccatg
     2761 ccagaaggtt atgttcaaga aagaactatt tttttcaaag atgacggtaa ctacaagacc
     2821 agagctgaag tcaagtttga aggtgatacc ttagttaata gaatcgaatt aaaaggtatt
     2881 gattttaaag aagatggtaa cattttaggt cacaaattgg aatacaacta taactctcac
     2941 aatgtttaca tcatggctga caaacaaaag aatggtatca aagttaactt caaaattaga
     3001 cacaacattg aagacggttc tgttcaatta gctgaccatt atcaacaaaa tactccaatt
     3061 ggtgatggtc cagtcttgtt accagacaac cattacttat ccactcaatc tgccttatcc
     3121 aaggatccaa acgaaaagag agaccacatg gtcttgttag aatttgttac tgctgctggt
     3181 attacctaat gagcttgtcg agaagtacta gaggatcata atcagccata ccacatttgt
     3241 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     3301 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     3361 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     3421 caaactcatc aatgtatctt atcatgtctg gatctgatca ctgcttgagc ctagaagatc
     3481 cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac
     3541 tatcataacc cctagggtat acccatctaa ttggaaccag ataagtgaaa tctagttcca
     3601 aactattttg tcatttttaa ttttcgtatt agcttacgac gctacaccca gttcccatct
     3661 attttgtcac tcttccctaa ataatcctta aaaactccat ttccacccct cccagttccc
     3721 aactattttg tccgcccaca accggtggag gaaattctcc ttgaagtttc cctggtgttc
     3781 aaagtaaagg agtttgcacc agacgcacct ctgttcactg gtccggcgta ttaaaacacg
     3841 atacattgtt attagtacat ttattaagcg ctagattctg tgcgttgttg atttacagac
     3901 aattgttgta cgtattttaa taattcatta aatttataat ctttagggtg gtatgttaga
     3961 gcgaaaatca aatgattttc agcgtcttta tatctgaatt taaatattaa atcctcaata
     4021 gatttgtaaa ataggtttcg attagtttca aacaagggtt gtttttccga accgatggct
     4081 ggactatcta atggattttc gctcaacgcc acaaaacttg ccaaatcttg tagcagcaat
     4141 ctagctttgt cgatattcgt ttgtgttttg ttttgtaata aaggttcgac gtcgttcaaa
     4201 atattatgcg cttttgtatt tctttcatca ctgtcgttag tgtacaattg actcgacgta
     4261 aacacgttaa atagagcttg gacatattta acatcgggcg tgttagcttt attaggccga
     4321 ttatcgtcgt cgtcccaacc ctcgtcgtta gaagttgctt ccgaagacga ttttgccata
     4381 gccacacgac gcctattaat tgtgtcggct aacacgtccg cgatcaaatt tgtagttgag
     4441 ctttttggaa ttaccggttg acttgggtca actgtcagac caagtttact catatatact
     4501 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     4561 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     4621 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     4681 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     4741 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     4801 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     4861 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     4921 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     4981 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     5041 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     5101 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     5161 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     5221 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     5281 tgctcacatg ttctttcctg cgttatcccc tgattgactt gggtcgctct tcctgtggat
     5341 gcgcaggtat gtacaggaag aggtttatac taaactgtta cattgcaaac gtggtttcgt
     5401 gtgccaagtg tgaaaaccga tgtttaatca aggctctgac gcatttctac aaccacgact
     5461 ctaagtgtgt gggtgaagtc atgcatcttt taatcaaatc ccaagatgtg tataaaccac
     5521 caaactgcca aaaaatgaaa actgtcgaca agctctgtcc gtttgctggc aactgcaagg
     5581 gtctcaatcc tatttgtaat tattgaataa taaaacaatt ataaatgtca aatttgtttt
     5641 ttattaacga tacaaaccaa acgcaacaag aacatttgta gtattatcta taattgaaaa
     5701 cgcgtagtta taatcgctga ggtaatattt aaaatcattt tcaaatgatt cacagttaat
     5761 ttgcgacaat ataattttat tttcacataa actagacgcc ttgtcgtctt cttcttcgta
     5821 ttccttctct ttttcatttt tctcttcata aaaattaaca tagttattat cgtatccata
     5881 tatgtatcta tcgtatagag taaatttttt gttgtcataa atatatatgt cttttttaat
     5941 ggggtgtata gtaccgctgc gcatagtttt tctgtaattt acaacagtgc tattttctgg
     6001 tagttcttcg gagtgtgttg ctttaattat taaatttata taatcaatga atttgggatc
     6061 gtcggttttg tacaatatgt tgccggcata gtacgcagct tcttctagtt caattacacc
     6121 attttttagc agcaccggat taacataact ttccaaaatg ttgtacgaac cgttaaacaa
     6181 aaacagttca cctccctttt ctatactatt gtctgcgagc agttgtttgt tgttaaaaat
     6241 aacagccatt gtaatgagac gcacaaacta atatcacaaa ctggaaatgt ctatcaatat
     6301 atagttgctg attgcgcaga tgccctgcgt aagcgggtgt gggcggacaa taaagtctta
     6361 aactgaacaa aatagatcta aactatgaca ataaagtctt aaactagaca gaatagttgt
     6421 aaactgaaat cagtccagtt atgctgtgaa aaagcatact ggacttttgt tatggctaaa
     6481 gcaaactctt cattttctga agtgcaaatt gcccgtcgta ttaaagaggg gcgtggccaa
     6541 gggcatgtaa agactatatt cgcggcgttg tgacaattta ccgaacaact ccgcggccgg
     6601 gaagccgatc tcggcttgaa cgaattgtta ggtggcggta cttgggtcga tatcaaagtg
     6661 catcacttct tcccgtatgc ccaactttgt atagagagcc actgcgggat cgtcaccgta
     6721 atctgcttgc acgtagatca cataagcacc aagcgcgttg gcctcatgct tgaggagatt
     6781 gatgagcgcg gtggcaatgc cctgcctccg gtgctcgccg gagactgcga gatcatagat
     6841 atagatctca ctacgcggct gctcaaactt gggcagaacg taagccgcga gagcgccaac
     6901 aaccgcttct tggtcgaagg cagcaagcgc gatgaatgtc ttactacgga gcaagttccc
     6961 gaggtaatcg gagtccggct gatgttggga gtaggtggct acgtctccga actcacgacc
     7021 gaaaagatca agagcagccc gcatggattt gacttggtca gggccgagcc tacatgtgcg
     7081 aatgatgccc atacttgagc cacctaactt tgttttaggg cgactgccct gctgcgtaac
     7141 atcgttgctg ctgcgtaaca tcgttgctgc tccataacat caaacatcga cccacggcgt
     7201 aacgcgcttg ctgcttggat gcccgaggca tagactgtac aaaaaaacag tcataacaag
     7261 ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt
     7321 tgcgtgagcg catacgctac ttgcattaca gtttacgaac cgaacaggct tatgtcaact
     7381 gggttcgtgc cttcatccgt ttccacggtg tgcgtcaccc ggcaaccttg ggcagcagcg
     7441 aagtcgccat aacttcgtat agcatacatt atacgaagtt atctg
//
