LOCUS       sequence-210423-        7341 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  N-terminal SNAPf tag on BICDR1 for expression in Sf9 cells. 
            8xHis-ZZ-SNAPf N-terminal tag, TEV site to cleave 8xHis-ZZ..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7341)
  AUTHORS   Urnavicius L, Lau CK, Elshenawy MM, Morales-Rios E, Motz C, Yildiz 
            A, Carter AP
  TITLE     Cryo-EM shows how dynactin recruits two dyneins for faster movement.
  JOURNAL   Nature. 2018 Feb 7;554(7691):202-206. doi: 10.1038/nature25462.
  PUBMED    29420470
REFERENCE   2  (bases 1 to 7341)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2018-02-7- 7"; volume: "554"; issue: "7691"; pages:
            "202-206"
COMMENT     Sequence Label: His-ZZ-TEV-SNAPf-BICDR1
FEATURES             Location/Qualifiers
     source          1..7341
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(92..625)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(814..819,820..836,837..842))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(913..946)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        1023..1114
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     1039..1056
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             1181..1204
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             1247..1420
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             1421..1594
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             1652..1672
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1694..1714
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1715..2257
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="DKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVEV
                     PAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLKV
                     VKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLAV
                     KEWLLAHEGHRLGKPGLG"
     polyA_signal    4125..4259
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4162..4181)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4216..4235
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  4380..4545
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     misc_recomb     4552..5257
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     3'UTR           4640..5012
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     CDS             complement(4669..5257)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR
                     PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR
                     LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF
                     ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     rep_origin      5447..6035
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5936..5955
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_recomb     6287..7114
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             6287..6411
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(6449..7054)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     mobile_element  join(7143..7341,1..25)
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
ORIGIN
        1 ccaagggcat gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg
       61 ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa
      121 agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac
      181 cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga
      241 gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat
      301 agatatagat ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc
      361 caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt
      421 tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac
      481 gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg
      541 tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg
      601 taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg
      661 gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa
      721 caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac
      781 cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc
      841 aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc
      901 agcgaagtcg ccataacttc gtatagcata cattatacga agttatctgt aactataacg
      961 gtcctaaggt agcgagttta aacactagta tcgattcgcg acctactccg gaatattaat
     1021 agatcatgga gataattaaa atgataacca tctcgcaaat aaataagtat tttactgttt
     1081 tcgtaacagt tttgtaataa aaaaacctat aaatattccg gattattcat accgtcccac
     1141 catcgggcgc ggatctcggt ccgaaaccat gtcgtactac catcaccatc accatcacca
     1201 tcacggttct atgtgcatgg caggccttgc gcaacacgat gaagccgtgg acaacaaatt
     1261 caacaaagaa caacaaaacg cgttctatga gatcttacat ttacctaact taaacgaaga
     1321 acaacgaaac gccttcatcc aaagtttaaa agatgaccca agccaaagcg ctaacctttt
     1381 agcagaagct aaaaagctaa atgatgctca ggcgccgaaa gtagacaaca aattcaacaa
     1441 agaacaacaa aacgcgttct atgagatctt acatttacct aacttaaacg aagaacaacg
     1501 aaacgccttc atccaaagtt taaaagatga cccaagccaa agcgctaacc ttttagcaga
     1561 agctaaaaag ctaaatgatg ctcaggcgcc gaaagtagac gcaaattccg cggggaagtc
     1621 aaccgattat gatattccaa ctactgctag cgagaaccta tatttccagg gagattatga
     1681 tatccctacg actgagaatc tttattttca gggcgacaaa gactgcgaaa tgaagcgcac
     1741 caccctggat agccctctgg gcaagctgga actgtctggg tgcgaacagg gcctgcaccg
     1801 tatcatcttc ctgggcaaag gaacatctgc cgccgacgcc gtggaagtgc ctgccccagc
     1861 cgccgtgctg ggcggaccag agccactgat gcaggccacc gcctggctca acgcctactt
     1921 tcaccagcct gaggccatcg aggagttccc tgtgccagcc ctgcaccacc cagtgttcca
     1981 gcaggagagc tttacccgcc aggtgctgtg gaaactgctg aaagtggtga agttcggaga
     2041 ggtcatcagc tacagccacc tggccgccct ggccggcaat cccgccgcca ccgccgccgt
     2101 gaaaaccgcc ctgagcggaa atcccgtgcc cattctgatc ccctgccacc gggtggtgca
     2161 gggcgacctg gacgtggggg gctacgaggg cgggctcgcc gtgaaagagt ggctgctggc
     2221 ccacgagggc cacagactgg gcaagcctgg gctgggtggt tcttccgcct tctgcctggg
     2281 cttggccggc cgagcttcag cacccgccga gccggacagc gcctgctgca tggagctgcc
     2341 cgctggggcc ggggacgcag tccggagtcc cgccactgct gccgccctcg tctccttccc
     2401 cggaggtccc ggggaactgg aactggcctt agaggaggag ctggcgttgc tggcggccgg
     2461 ggagcggtcg tccgagcccg gcgagcatcc tcaggcggag ccggagtctc cggttgaggg
     2521 gcacgggccg ccgctgccgc cgccgcctac ccaggatccc gagctgctgt cggtgatccg
     2581 ccagaaggag aaggatctgg tgttggcggc ccgactaggc aaggcgctgc tcgagaggaa
     2641 ccaggacatg agccggcagt acgagcagat gcacaaggag ctgacggaca agctagagca
     2701 cctagaacaa gagaaacatg aactgagaag gcgattcgag aatcgagaag gggagtggga
     2761 aggacgggtg tctgagctgg agacggacgt gaagcagcta caggatgagt tggagaggca
     2821 gcagctccac cttcgggaag cagaccgaga aaaaacacga gctgtccagg aactgtcaga
     2881 acagaaccag aggctgctgg accaactcag cagggcatct gaagttgaga gacagctctc
     2941 catgcaggtc catgctctca aagaagactt ccgggagaaa aattcctcca ccaaccagca
     3001 tatcatccgg ctggagagtc tgcaggccga gatcaagatg ctgtcagatc gaaaacggga
     3061 gctggagcat cggctcagcg ccaccttgga ggagaatgac ctgcttcaag ggactgtgga
     3121 ggagctgcag gaccgggtgc tgatcctaga aaggcagggc catgacaaag acctacagct
     3181 gcaccaaagc cagctggagc tccaggaggt gcggctgtcc taccggcagc tgcagggcaa
     3241 ggtggaggaa ctcacggagg agaggagttt gcagagctcg gctgcgacca gcacgtccct
     3301 gctgtcagag atcgagcaga gcatggaggc cgaggagctg gagcaggaga gagagcagct
     3361 gaggctgcag ctctgggaag cctactgcca ggttcgatat ctctgctcac acctccgagg
     3421 gaatgacagt gccgactcag ccgtatccac agactcctcc atggacgagt cctcggagac
     3481 ctcgtcggcc aaggacgtgc ctgccggcag cctgcgcact gccctcaacg atctcaagag
     3541 gctgatacag agcatcgtgg acggtgtgga gcccacggtg acactgctga gcgtggagat
     3601 gaccgcactg aaggaggaaa gggaccgact cagagtgacc tctgaggaca aggagccaaa
     3661 ggagcagctt cagaaggcca tcagggaccg ggacgaggcc attgccaaga agaacgctgt
     3721 ggagctggaa ctcgccaagt gcaagatgga tatgatgtct ctgaacagcc agctactgga
     3781 tgccattcag caaaaactga acctctctca gcagctggag gcttggcagg atgacatgca
     3841 cagggtcatt gacaggcagc tgatggacac gcacctgaag gaacagagcc ggcctgctgc
     3901 tgctgccttc cctaggggcc acggcgtagg gcgggggcag gaacccagca ccgcagatgg
     3961 caaacggctc ttctccttct tcaggaagat ttaatgagct tgtcgagaag tactagagga
     4021 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     4081 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
     4141 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     4201 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatct
     4261 gatcactgct tgagcctaga agatccggct gctaacaaag cccgaaagga agctgagttg
     4321 gctgctgcca ccgctgagca ataactatca taacccctag ggtataccca tctaattgga
     4381 accagataag tgaaatctag ttccaaacta ttttgtcatt tttaattttc gtattagctt
     4441 acgacgctac acccagttcc catctatttt gtcactcttc cctaaataat ccttaaaaac
     4501 tccatttcca cccctcccag ttcccaacta ttttgtccgc ccacaaccgg tggaggaaat
     4561 tctccttgaa gtttccctgg tgttcaaagt aaaggagttt gcaccagacg cacctctgtt
     4621 cactggtccg gcgtattaaa acacgataca ttgttattag tacatttatt aagcgctaga
     4681 ttctgtgcgt tgttgattta cagacaattg ttgtacgtat tttaataatt cattaaattt
     4741 ataatcttta gggtggtatg ttagagcgaa aatcaaatga ttttcagcgt ctttatatct
     4801 gaatttaaat attaaatcct caatagattt gtaaaatagg tttcgattag tttcaaacaa
     4861 gggttgtttt tccgaaccga tggctggact atctaatgga ttttcgctca acgccacaaa
     4921 acttgccaaa tcttgtagca gcaatctagc tttgtcgata ttcgtttgtg ttttgttttg
     4981 taataaaggt tcgacgtcgt tcaaaatatt atgcgctttt gtatttcttt catcactgtc
     5041 gttagtgtac aattgactcg acgtaaacac gttaaataga gcttggacat atttaacatc
     5101 gggcgtgtta gctttattag gccgattatc gtcgtcgtcc caaccctcgt cgttagaagt
     5161 tgcttccgaa gacgattttg ccatagccac acgacgccta ttaattgtgt cggctaacac
     5221 gtccgcgatc aaatttgtag ttgagctttt tggaattacc ggttgacttg ggtcaactgt
     5281 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     5341 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     5401 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     5461 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     5521 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     5581 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     5641 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     5701 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     5761 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     5821 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     5881 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     5941 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     6001 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     6061 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     6121 gacttgggtc gctcttcctg tggatgcgca ggtatgtaca ggaagaggtt tatactaaac
     6181 tgttacattg caaacgtggt ttcgtgtgcc aagtgtgaaa accgatgttt aatcaaggct
     6241 ctgacgcatt tctacaacca cgactctaag tgtgtgggtg aagtcatgca tcttttaatc
     6301 aaatcccaag atgtgtataa accaccaaac tgccaaaaaa tgaaaactgt cgacaagctc
     6361 tgtccgtttg ctggcaactg caagggtctc aatcctattt gtaattattg aataataaaa
     6421 caattataaa tgtcaaattt gttttttatt aacgatacaa accaaacgca acaagaacat
     6481 ttgtagtatt atctataatt gaaaacgcgt agttataatc gctgaggtaa tatttaaaat
     6541 cattttcaaa tgattcacag ttaatttgcg acaatataat tttattttca cataaactag
     6601 acgccttgtc gtcttcttct tcgtattcct tctctttttc atttttctct tcataaaaat
     6661 taacatagtt attatcgtat ccatatatgt atctatcgta tagagtaaat tttttgttgt
     6721 cataaatata tatgtctttt ttaatggggt gtatagtacc gctgcgcata gtttttctgt
     6781 aatttacaac agtgctattt tctggtagtt cttcggagtg tgttgcttta attattaaat
     6841 ttatataatc aatgaatttg ggatcgtcgg ttttgtacaa tatgttgccg gcatagtacg
     6901 cagcttcttc tagttcaatt acaccatttt ttagcagcac cggattaaca taactttcca
     6961 aaatgttgta cgaaccgtta aacaaaaaca gttcacctcc cttttctata ctattgtctg
     7021 cgagcagttg tttgttgtta aaaataacag ccattgtaat gagacgcaca aactaatatc
     7081 acaaactgga aatgtctatc aatatatagt tgctgattgc gcagatgccc tgcgtaagcg
     7141 ggtgtgggcg gacaataaag tcttaaactg aacaaaatag atctaaacta tgacaataaa
     7201 gtcttaaact agacagaata gttgtaaact gaaatcagtc cagttatgct gtgaaaaagc
     7261 atactggact tttgttatgg ctaaagcaaa ctcttcattt tctgaagtgc aaattgcccg
     7321 tcgtattaaa gaggggcgtg g
//
