LOCUS       sequence-210427-        6813 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  N-terminal SNAPf tag on BICD2N (1-400) for expression in Sf9 cells. 
            8xHis-ZZ-SNAPf N-terminal tag, TEV site to cleave 8xHis-ZZ..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6813)
  AUTHORS   Urnavicius L, Lau CK, Elshenawy MM, Morales-Rios E, Motz C, Yildiz 
            A, Carter AP
  TITLE     Cryo-EM shows how dynactin recruits two dyneins for faster movement.
  JOURNAL   Nature. 2018 Feb 7;554(7691):202-206. doi: 10.1038/nature25462.
  PUBMED    29420470
REFERENCE   2  (bases 1 to 6813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2018-02-7- 7"; volume: "554"; issue: "7691"; pages:
            "202-206"
COMMENT     Sequence Label: His-ZZ-TEV-SNAPf-BICD2N(1-400)
FEATURES             Location/Qualifiers
     source          1..6813
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        74..165
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     90..107
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             232..255
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             298..471
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             472..645
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             703..723
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             745..765
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             766..1308
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="DKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVEV
                     PAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLKV
                     VKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLAV
                     KEWLLAHEGHRLGKPGLG"
     CDS             1800..1811
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2648..2782
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2685..2704)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2739..2758
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  2903..3068
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     misc_recomb     3075..3780
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     3'UTR           3163..3535
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     CDS             complement(3192..3780)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR
                     PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR
                     LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF
                     ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     rep_origin      3970..4558
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4459..4478
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_recomb     4810..5637
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             4810..4934
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(4972..5577)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     mobile_element  5666..5889
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(5956..6489)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(6678..6683,6684..6700,6701..6706))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(6777..6810)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 taactataac ggtcctaagg tagcgagttt aaacactagt atcgattcgc gacctactcc
       61 ggaatattaa tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta
      121 ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca
      181 taccgtccca ccatcgggcg cggatctcgg tccgaaacca tgtcgtacta ccatcaccat
      241 caccatcacc atcacggttc tatgtgcatg gcaggccttg cgcaacacga tgaagccgtg
      301 gacaacaaat tcaacaaaga acaacaaaac gcgttctatg agatcttaca tttacctaac
      361 ttaaacgaag aacaacgaaa cgccttcatc caaagtttaa aagatgaccc aagccaaagc
      421 gctaaccttt tagcagaagc taaaaagcta aatgatgctc aggcgccgaa agtagacaac
      481 aaattcaaca aagaacaaca aaacgcgttc tatgagatct tacatttacc taacttaaac
      541 gaagaacaac gaaacgcctt catccaaagt ttaaaagatg acccaagcca aagcgctaac
      601 cttttagcag aagctaaaaa gctaaatgat gctcaggcgc cgaaagtaga cgcaaattcc
      661 gcggggaagt caaccgatta tgatattcca actactgcta gcgagaacct atatttccag
      721 ggagattatg atatccctac gactgagaat ctttattttc agggcgacaa agactgcgaa
      781 atgaagcgca ccaccctgga tagccctctg ggcaagctgg aactgtctgg gtgcgaacag
      841 ggcctgcacc gtatcatctt cctgggcaaa ggaacatctg ccgccgacgc cgtggaagtg
      901 cctgccccag ccgccgtgct gggcggacca gagccactga tgcaggccac cgcctggctc
      961 aacgcctact ttcaccagcc tgaggccatc gaggagttcc ctgtgccagc cctgcaccac
     1021 ccagtgttcc agcaggagag ctttacccgc caggtgctgt ggaaactgct gaaagtggtg
     1081 aagttcggag aggtcatcag ctacagccac ctggccgccc tggccggcaa tcccgccgcc
     1141 accgccgccg tgaaaaccgc cctgagcgga aatcccgtgc ccattctgat cccctgccac
     1201 cgggtggtgc agggcgacct ggacgtgggg ggctacgagg gcgggctcgc cgtgaaagag
     1261 tggctgctgg cccacgaggg ccacagactg ggcaagcctg ggctgggtgg ttctatgtca
     1321 gctccatcag aagaagagga atatgctagg ttggtcatgg aagcccagcc cgagtggctc
     1381 agggctgaag ttaagcgcct gtctcacgag ctcgccgaga caaccagaga gaagatccaa
     1441 gcagctgaat atggcctcgc tgttttggag gagaaacacc aactgaaact ccagttcgag
     1501 gagctggagg ttgactacga ggccattagg tcagaaatgg agcaactgaa agaagccttc
     1561 ggccaagcac ataccaatca taagaaggtt gccgcagacg gagagtccag agaagaatca
     1621 ctgatccagg agtctgctag caaggagcag tattacgtga ggaaagtcct ggagctgcag
     1681 accgagttga agcaactcag gaacgttctg accaacaccc aatctgaaaa cgagagattg
     1741 acaagcgtgg cccaagagct caaggaaatc aatcagaacg tcgagattca acgcggtaga
     1801 ttgagggacg acatcaaaga atacaaattc agggaggcaa gactcctcca agactactca
     1861 gagctggaag aggagaacat ctctctgcag aaacaagtct cagtgctcag gcaaaatcag
     1921 gtggagttcg aaggactgaa gcacgaaatc aagcgcctcg aagaggagac agagtacctg
     1981 aactcccaac tggaggacgc tatcagactg aaggagattt cagaaagaca gctcgaagaa
     2041 gccctggaaa cactgaagac agaaagggag cagaagaata acctccgcaa ggagttgagc
     2101 cactatatgt ctatcaacga ttccttctac acttcacatc tccaggtcag cttggacgga
     2161 ctgaagttca gcgacgacac tgttaccgcc gagcccaaca atgatgccga agctctcgtg
     2221 aacggattcg aacatagcgg cctggtcaaa tcctcattgg ataacaagac tagcactcca
     2281 cgcaaggatg gtctggctcc tccctctcct tccttggtgt ccgatctgtt gagcgagttg
     2341 cacatcagcg aaatccagaa gctcaagcag cagctcgtcc aaatggagcg cgagaaggtg
     2401 ggtctgctgg ccacattgca agatactcag aaacagttgg agcaggctag aggtaccttg
     2461 tcagagcagc atgagaaggt gaacaggttg accgagaatc tgagcgctct gaggtaatga
     2521 gcttgtcgag aagtactaga ggatcataat cagccatacc acatttgtag aggttttact
     2581 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     2641 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     2701 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     2761 tgtatcttat catgtctgga tctgatcact gcttgagcct agaagatccg gctgctaaca
     2821 aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta tcataacccc
     2881 tagggtatac ccatctaatt ggaaccagat aagtgaaatc tagttccaaa ctattttgtc
     2941 atttttaatt ttcgtattag cttacgacgc tacacccagt tcccatctat tttgtcactc
     3001 ttccctaaat aatccttaaa aactccattt ccacccctcc cagttcccaa ctattttgtc
     3061 cgcccacaac cggtggagga aattctcctt gaagtttccc tggtgttcaa agtaaaggag
     3121 tttgcaccag acgcacctct gttcactggt ccggcgtatt aaaacacgat acattgttat
     3181 tagtacattt attaagcgct agattctgtg cgttgttgat ttacagacaa ttgttgtacg
     3241 tattttaata attcattaaa tttataatct ttagggtggt atgttagagc gaaaatcaaa
     3301 tgattttcag cgtctttata tctgaattta aatattaaat cctcaataga tttgtaaaat
     3361 aggtttcgat tagtttcaaa caagggttgt ttttccgaac cgatggctgg actatctaat
     3421 ggattttcgc tcaacgccac aaaacttgcc aaatcttgta gcagcaatct agctttgtcg
     3481 atattcgttt gtgttttgtt ttgtaataaa ggttcgacgt cgttcaaaat attatgcgct
     3541 tttgtatttc tttcatcact gtcgttagtg tacaattgac tcgacgtaaa cacgttaaat
     3601 agagcttgga catatttaac atcgggcgtg ttagctttat taggccgatt atcgtcgtcg
     3661 tcccaaccct cgtcgttaga agttgcttcc gaagacgatt ttgccatagc cacacgacgc
     3721 ctattaattg tgtcggctaa cacgtccgcg atcaaatttg tagttgagct ttttggaatt
     3781 accggttgac ttgggtcaac tgtcagacca agtttactca tatatacttt agattgattt
     3841 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     3901 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     3961 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     4021 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     4081 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     4141 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     4201 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     4261 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     4321 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     4381 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     4441 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     4501 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     4561 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     4621 ctttcctgcg ttatcccctg attgacttgg gtcgctcttc ctgtggatgc gcaggtatgt
     4681 acaggaagag gtttatacta aactgttaca ttgcaaacgt ggtttcgtgt gccaagtgtg
     4741 aaaaccgatg tttaatcaag gctctgacgc atttctacaa ccacgactct aagtgtgtgg
     4801 gtgaagtcat gcatctttta atcaaatccc aagatgtgta taaaccacca aactgccaaa
     4861 aaatgaaaac tgtcgacaag ctctgtccgt ttgctggcaa ctgcaagggt ctcaatccta
     4921 tttgtaatta ttgaataata aaacaattat aaatgtcaaa tttgtttttt attaacgata
     4981 caaaccaaac gcaacaagaa catttgtagt attatctata attgaaaacg cgtagttata
     5041 atcgctgagg taatatttaa aatcattttc aaatgattca cagttaattt gcgacaatat
     5101 aattttattt tcacataaac tagacgcctt gtcgtcttct tcttcgtatt ccttctcttt
     5161 ttcatttttc tcttcataaa aattaacata gttattatcg tatccatata tgtatctatc
     5221 gtatagagta aattttttgt tgtcataaat atatatgtct tttttaatgg ggtgtatagt
     5281 accgctgcgc atagtttttc tgtaatttac aacagtgcta ttttctggta gttcttcgga
     5341 gtgtgttgct ttaattatta aatttatata atcaatgaat ttgggatcgt cggttttgta
     5401 caatatgttg ccggcatagt acgcagcttc ttctagttca attacaccat tttttagcag
     5461 caccggatta acataacttt ccaaaatgtt gtacgaaccg ttaaacaaaa acagttcacc
     5521 tcccttttct atactattgt ctgcgagcag ttgtttgttg ttaaaaataa cagccattgt
     5581 aatgagacgc acaaactaat atcacaaact ggaaatgtct atcaatatat agttgctgat
     5641 tgcgcagatg ccctgcgtaa gcgggtgtgg gcggacaata aagtcttaaa ctgaacaaaa
     5701 tagatctaaa ctatgacaat aaagtcttaa actagacaga atagttgtaa actgaaatca
     5761 gtccagttat gctgtgaaaa agcatactgg acttttgtta tggctaaagc aaactcttca
     5821 ttttctgaag tgcaaattgc ccgtcgtatt aaagaggggc gtggccaagg gcatgtaaag
     5881 actatattcg cggcgttgtg acaatttacc gaacaactcc gcggccggga agccgatctc
     5941 ggcttgaacg aattgttagg tggcggtact tgggtcgata tcaaagtgca tcacttcttc
     6001 ccgtatgccc aactttgtat agagagccac tgcgggatcg tcaccgtaat ctgcttgcac
     6061 gtagatcaca taagcaccaa gcgcgttggc ctcatgcttg aggagattga tgagcgcggt
     6121 ggcaatgccc tgcctccggt gctcgccgga gactgcgaga tcatagatat agatctcact
     6181 acgcggctgc tcaaacttgg gcagaacgta agccgcgaga gcgccaacaa ccgcttcttg
     6241 gtcgaaggca gcaagcgcga tgaatgtctt actacggagc aagttcccga ggtaatcgga
     6301 gtccggctga tgttgggagt aggtggctac gtctccgaac tcacgaccga aaagatcaag
     6361 agcagcccgc atggatttga cttggtcagg gccgagccta catgtgcgaa tgatgcccat
     6421 acttgagcca cctaactttg ttttagggcg actgccctgc tgcgtaacat cgttgctgct
     6481 gcgtaacatc gttgctgctc cataacatca aacatcgacc cacggcgtaa cgcgcttgct
     6541 gcttggatgc ccgaggcata gactgtacaa aaaaacagtc ataacaagcc atgaaaaccg
     6601 ccactgcgcc gttaccaccg ctgcgttcgg tcaaggttct ggaccagttg cgtgagcgca
     6661 tacgctactt gcattacagt ttacgaaccg aacaggctta tgtcaactgg gttcgtgcct
     6721 tcatccgttt ccacggtgtg cgtcacccgg caaccttggg cagcagcgaa gtcgccataa
     6781 cttcgtatag catacattat acgaagttat ctg
//
