LOCUS       sequence-210431-       10489 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For performing ORBIT - Expresses Che9c phage RecT and Bxb1 phage 
            Integrase, and contains SacB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10489)
  AUTHORS   Murphy KC, Nelson SJ, Nambi S, Papavinasasundaram K, Baer CE, 
            Sassetti CM
  TITLE     ORBIT: a New Paradigm for Genetic Engineering of Mycobacterial 
            Chromosomes.
  JOURNAL   MBio. 2018 Dec 11;9(6). pii: mBio.01467-18. doi: 
            10.1128/mBio.01467-18.
  PUBMED    30538179
REFERENCE   2  (bases 1 to 10489)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio."; 
            date: "2018-12-11"; volume: "9(6). pii: mBio.01467-18. doi"; pages: 
            " 10.1128/mBio.01467-18"
COMMENT     Sequence Label: pKM461
FEATURES             Location/Qualifiers
     source          1..10489
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      20..47
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             1533..3035
                     /codon_start=1
                     /transl_table=11
                     /gene="Bxb1"
                     /product="Bxb1 integrase"
                     /label=Bxb1 integrase
                     /note="Bxb1 large serine integrase from Mycobacterium 
                     bacteriophage Bxb1 (Ghosh et al. 2003)."
                     /protein_id="AFF61396.1"
                     /translation="MRALVVIRLSRVTDATTSPERQLESCQQLCAQRGWDVVGVAEDLD
                     VSGAVDPFDRKRRPNLARWLAFEEQPFDVIVAYRVDRLTRSIRHLQQLVHWAEDHKKLV
                     VSATEAHFDTTTPFAAVVIALMGTVAQMELEAIKERNRSAAHFNIRAGKYRGSLPPWGY
                     LPTRVDGEWRLVPDPVQRERILEVYHRVVDNHEPLHLVAHDLNRRGVLSPKDYFAQLQG
                     REPQGREWSATALKRSMISEAMLGYATLNGKTVRDDDGAPLVRAEPILTREQLEALRAE
                     LVKTSRAKPAVSTPSLLLRVLFCAVCGEPAYKFAGGGRKHPRYRCRSMGFPKHCGNGTV
                     AMAEWDAFCEEQVLDLLGDAERLEKVWVAGSDSAVELAEVNAELVDLTSLIGSPAYRAG
                     SPQREALDARIAALAARQEELEGLEARPSGWEWRETGQRFGDWWREQDTAAKNTWLRSM
                     NVRLTFDVRGGLTRTIDFGDLQEYEQHLRLGSVVERLHTGMS"
     terminator      3070..3116
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(3249..3268)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3260..4075
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3333..3352)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     complement(4136..4152)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4137..4153)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             join(7319..7405,7406..8740)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     CDS             8973..9596
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(9046..9065)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     rep_origin      9660..10248
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10149..10168
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10402..10419
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 actagttgat tagctaagca gaaggccatc ctgacggatg gcctttttgc gtttaatact
       61 gtttaaactc tagaaatatt ggatcgtcgg caccgtcacg gccgtgggag gcggcacgat
      121 ccgcgacgtg atgatcggcc gcatccccac ggtgctgcgc agtgagctct acgccatccc
      181 ggcgttgatc tgtgcgttcg cacgcacagg cccggtgtga gaagggtctc tgacgagcgg
      241 gagaactccc tatcagtgat agagtttgtc ctccctatca gtgatagata ggctctggga
      301 gtacccgtgt gtacgaccag cacggcatac atcatttcga cgccgagaga ttcgccgccc
      361 gaaatgagca cgatccgcat gcttaattaa gaaggagata tacatatggc tgaaaatgct
      421 gtcaccaagc aggattcgcc caaggcaccc gagacgatct cgcaagtgct gcaggtgctc
      481 gtgccgcagc tggcgcgtgc agtgcccaag ggtatggacc ccgaccgcat cgcgcggatc
      541 gtgcaaaccg agatccgcaa gtcgcgcaac gcgaaagctg ctggcatcgc taagcagtcc
      601 ctcgacgact gcacgcaaga gtcatttgcc ggtgcgctgc tgacctcggc cgcgctcggt
      661 ctcgagcccg gtgtcaacgg cgagtgctac ctcgtgccct accgcgacac ccggcgcggc
      721 gtggtcgagt gccagctgat catcggctac cagggcatcg tcaaactgtt ctggcagcac
      781 ccgcgcgcct cccggatcga cgcgcagtgg gtcggcgcga acgacgaatt ccattacacc
      841 atgggcctca atccgacgct gaaacacgtg aaggccaagg gtgatcgggg taatccggtc
      901 tacttctacg cgatcgtcga ggtgaccggc gctgagccgc tgtgggacgt gttcaccgcc
      961 gacgagatca gggaattgcg tcgcggcaag gtcggatcct cgggcgacat caaggacccg
     1021 cagcgctgga tggagcggaa gaccgcgctc aaacaggtgc tgaagctggc accgaagacg
     1081 acgcggctcg acgcggcgat ccgcgccgac gatcgcccgg gcaccgacct gtcacagtcg
     1141 caggcgctcg cgctgccgtc gaccgtcaag ccgacggccg actacatcga cggcgagatc
     1201 gccgagccgc acgaggtcga tacgccaccg aagtcgtcgc gcgcacaacg cgcgcagcgc
     1261 gccaccgcgc cggcgcccga cgtgcagatg gccaatcccg atcagctgaa gcgcctcggc
     1321 gagatccaga aggccgaaaa gtacaacgac gccgattggt tcaagttcct cgccgattcg
     1381 gccggcgtca aagccacgcg cgccgccgac ctcacgttcg acgaggcgaa ggctgtcatc
     1441 gacatgttcg acgggcccaa cgcatgagcg ccccggcgaa ttccgacgcg gtggttgatc
     1501 tgcgctagtg gaggcgccag aggcacttcg agatgagagc cctggtagtc atccgcctgt
     1561 cccgcgtcac cgatgctacg acttcaccgg agcgtcagct ggagtcttgc cagcagctct
     1621 gcgcccagcg cggctgggac gtcgtcgggg tagcggagga tctggacgtc tccggggcgg
     1681 tcgatccgtt cgaccggaag cgcagaccga acctggcccg gtggctagcg ttcgaggagc
     1741 aaccgttcga cgtgatcgtg gcgtaccggg tagaccggtt gacccgatcg atccggcatc
     1801 tgcagcagct ggtccactgg gccgaggacc acaagaagct ggtcgtctcc gcgaccgaag
     1861 cgcacttcga tacgacgacg ccgtttgcgg cggtcgtcat cgcgcttatg ggaacggtgg
     1921 cgcagatgga attagaagcg atcaaagagc ggaaccgttc ggctgcgcat ttcaatatcc
     1981 gcgccgggaa ataccgagga tccctgccgc cgtggggata cctgcctacg cgcgtggacg
     2041 gggagtggcg gctggtgccg gaccctgtgc agcgagagcg catcctcgag gtgtatcacc
     2101 gcgtcgtcga caaccacgag ccgctgcacc tggtggccca cgacctgaac cggcgtggtg
     2161 tcctgtcgcc gaaggactac ttcgcgcagc tgcaaggccg cgagccgcag ggccgggagt
     2221 ggtcggctac cgcgctgaag cgatcgatga tctccgaggc gatgctcggg tacgcgactc
     2281 tgaacggtaa gaccgtccga gacgacgacg gagccccgct ggtgcgggct gagccgatcc
     2341 tgacccgtga gcagctggag gcgctgcgcg ccgagctcgt gaagacctcc cgggcgaagc
     2401 ccgcggtgtc taccccgtcg ctgctgctgc gggtgttgtt ctgcgcggtg tgcggggagc
     2461 ccgcgtacaa gttcgccggg ggaggacgta agcacccgcg ctaccgctgc cgctcgatgg
     2521 ggttcccgaa gcactgcggg aacggcacgg tggcgatggc cgagtgggac gcgttctgcg
     2581 aggagcaggt gctggatctg ctcggggacg cggagcgtct ggagaaagtc tgggtagccg
     2641 gctcggactc cgcggtcgaa ctcgcggagg tgaacgcgga gctggtggac ctgacgtcgc
     2701 tgatcggctc cccggcctac cgggccggct ctccgcagcg agaagcactg gatgcccgta
     2761 ttgcggcgct ggccgcgcgg caagaggagc tggagggtct agaggctcgc ccgtctggct
     2821 gggagtggcg cgagaccggg cagcggttcg gggactggtg gcgggagcag gacaccgcgg
     2881 caaagaacac ctggcttcgg tcgatgaacg ttcggctgac gttcgacgtc cgcggcgggc
     2941 tgactcgcac gatcgacttc ggggatctgc aggagtacga gcagcatctc aggctcggca
     3001 gcgtggtcga acggctacac accgggatgt cgtaggttaa caactagcgt acgatcgact
     3061 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatgcca
     3121 tcatggccgc gggacgtcgg ggggggggga aagccacgtt gtgtctcaaa atctctgatg
     3181 ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa
     3241 cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcttgct cgaggccgcg
     3301 attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg
     3361 gcaatcaggt gcgacaatct atcgcttgta tgggaagccc catgcgccag agttgtttct
     3421 gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg
     3481 gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc
     3541 atggttactc accactgcga tccccgggaa aacagcattc caggtattag aagaatatcc
     3601 tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat
     3661 tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc
     3721 acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc
     3781 tgttgaacaa gtctggaaag aaatgcataa tcttttgcca ttctcaccgg attcagtcgt
     3841 cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg
     3901 tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa
     3961 ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga
     4021 taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga
     4081 tatcaagctt tagctaatta attggggacc ctagaggtcc ccttttttat tttatcgata
     4141 ccgtcgacct cgaggggggg cccccgggga tcatcgagcc gagaacgtta tcgaagttgg
     4201 tcatgtgtaa tcccctcgtt tgaactttgg attaagcgta gatacaccct tggacaagcc
     4261 agttggattc ggagacaagc aaattcagcc ttaaaaaggg cgaggccctg cggtggtgga
     4321 acaccgcagg gcctctaacc gctcgacgcg ctgcaccaac cagcccgcga acggctggca
     4381 gccagcgtaa ggcgcggctc atcgggcggc gttcgccacg atgtcctgca cttcgagcca
     4441 agcctcgaac acctgctggt gtgcacgact cacccggttg ttgacaccgc gcgcggccgt
     4501 gcgggctcgg tggggcggct ctgtcgccct tgccagcgtg agtagcgcgt acctcacctc
     4561 gcccaacagg tcgcacacag ccgattcgta cgccataaag ccaggtgagc ccaccagctc
     4621 cgtaagttcg ggcgctgtgt ggctcgtacc cgcgcattca ggcggcaggg ggtctaacgg
     4681 gtctaaggcg gcgtgtacgg ccgccacagc ggctctcagc ggcccggaaa cgtcctcgaa
     4741 acgacgcatg tgttcctcct ggttggtaca ggtggttggg ggtgctcggc tgtcgctggt
     4801 gttccaccac cagggctcga cgggagagcg ggggagtgtg cagttgtggg gtggcccctc
     4861 agcgaaatat ctgacttgga gctcgtgtcg gaccatacac cggtgattaa tcgtggtcta
     4921 ctaccaagcg tgagccacgt cgccgacgaa tttgagcagc tctggctgcc gtactggccg
     4981 ctggcaagcg acgatctgct cgaggggatc taccgccaaa gccgcgcgtc ggccctaggc
     5041 cgccggtaca tcgaggcgaa cccaacagcg ctggcaaacc tgctggtcgt ggacgtagac
     5101 catccagacg cagcgctccg agcgctcagc gcccgggggt cccatccgct gcccaacgcg
     5161 atcgtgggca atcgcgccaa cggccacgca cacgcagtgt gggcactcaa cgcccctgtt
     5221 ccacgcaccg aatacgcgcg gcgtaagccg ctcgcataca tggcggcgtg cgccgaaggc
     5281 cttcggcgcg ccgtcgacgg cgaccgcagt tactcaggcc tcatgaccaa aaaccccggc
     5341 cacatcgcct gggaaacgga atggctccac tcagatctct acacactcag ccacatcgag
     5401 gccgagctcg gcgcgaacat gccaccgccg cgctggcgtc agcagaccac gtacaaagcg
     5461 gctccgacgc cgctagggcg gaattgcgca ctgttcgatt ccgtcaggtt gtgggcctat
     5521 cgtcccgccc tcatgcggat ctacctgccg acccggaacg tggacggact cggccgcgcg
     5581 atctatgccg agtgccacgc gcgaaacgcc gaattcccgt gcaacgacgt gtgtcccgga
     5641 ccgctaccgg acagcgaggt ccgcgccatc gccaacagca tttggcgttg gatcacaacc
     5701 aagtcgcgca tttgggcgga cgggatcgtg gtctacgagg ccacactcag tgcgcgccag
     5761 tcggccatct cgcggaaggg cgcagcagcg cgcacggcgg cgagcacagt tgcgcggcgc
     5821 gcaaagtccg cgtcagccat ggaggcattg ctatgagcga cggctacagc gacggctaca
     5881 accggcagcc gactgtccgc aaaaagcggc gcgtgaccgc cgccgaaggc gctcgaatca
     5941 ccggactatc cgaacgccac gtcgtccggc tcgtggcgca ggaacgcagc gagtggctcg
     6001 ccgagcaggc tgcacgccgc gaacgcatcc gcgcctatca cgacgacgag ggccactctt
     6061 ggccgcaaac ggccaaacat ttcgggctgc atctggacac cgttaagcga ctcggctatc
     6121 gggcgaggaa agagcgtgcg gcagaacagg aagcggctca aaaggcccac aacgaagccg
     6181 acaatccacc gctgttctaa cgcaattggg gagcgggtgt cgcgggggtt ccgtgggggg
     6241 ttccgttgca acgggtcgga caggtaaaag tcctggtaga cgctagtttt ctggtttggg
     6301 ccatgcctgt ctcgttgcgt gtttcgttgc gcccgttttg aataccagcc agacgagacg
     6361 gggttctacg aatcttggtc gataccaagc catttccgct gaatatcggg gagctcaccg
     6421 ccagaatcgg tggttgtggt gatgtacgtg gcgaactccg ttgtagtgcc tgtggtggca
     6481 tccgtggcca ctctcgttgc acggttcgtt gtgccgttac aggccccgtt gacagctcac
     6541 cgaacgtagt taaaacatgc tggtcaaact aggtttacca acgatacgag tcagctcatc
     6601 tagggccagt tctaggcgtt gttcgttgcg cggttcgttg cgcatgtttc gtgtggttgc
     6661 tagatggctc cgcaaccaca cgcttcgagg ttgagtgctt ccagcacggg cgcgatccag
     6721 aagaacttcg tcgtgcgact gtcctcgttg atccttgccg agctgggatg gaagcccggc
     6781 cgacccaccc tggaggagat gatcgaggat gccagggcct ttcacgcccg ccgctgctga
     6841 gcgtccgccg ccgggcccgc accgccgtcg gccggcccgc tccgggctcg cagcagcggg
     6901 cttcggcgcg ggcccggggc tcccgggccg ccgggcgggg ctccgcccgg cggccgccca
     6961 tgcaacagaa actataaaaa atacagagaa tgaaaagaaa cagatagatt ttttagttct
     7021 ttaggcccgt agtctgcaaa tccttttatg attttctatc aaacaaaaga ggaaaataga
     7081 ccagttgcaa tccaaacgag agtctaatag aatgaggtcg aaaagtaaat cgcgcgggtt
     7141 tgttactgat aaagcaggca agacctaaaa tgtgtaaagg gcaaagtgta tactttggcg
     7201 tcacccctta catattttag gtcttttttt attgtgcgta actaacttgc catcttcaaa
     7261 caggagggct ggaagaagca gaccgctaac acagtacata aaaaaggaga catgaacgat
     7321 gaacatcaaa aagtttgcaa aacaagcaac agtattaacc tttactaccg cactgctggc
     7381 aggaggcgca actcaagcgt ttgcgaaaga aacgaaccaa aagccatata aggaaacata
     7441 cggcatttcc catattacac gccatgatat gctgcaaatc cctgaacagc aaaaaaatga
     7501 aaaatatcaa gttcctgaat tcgattcgtc cacaattaaa aatatctctt ctgcaaaagg
     7561 cctggacgtt tgggacagct ggccattaca aaacgctgac ggcactgtcg caaactatca
     7621 cggctaccac atcgtctttg cattagccgg agatcctaaa aatgcggatg acacatcgat
     7681 ttacatgttc tatcaaaaag tcggcgaaac ttctattgac agctggaaaa acgctggccg
     7741 cgtctttaaa gacagcgaca aattcgatgc aaatgattct atcctaaaag accaaacaca
     7801 agaatggtca ggttcagcca catttacatc tgacggaaaa atccgtttat tctacactga
     7861 tttctccggt aaacattacg gcaaacaaac actgacaact gcacaagtta acgtatcagc
     7921 atcagacagc tctttgaaca tcaacggtgt agaggattat aaatcaatct ttgacggtga
     7981 cggaaaaacg tatcaaaatg tacagcagtt catcgatgaa ggcaactaca gctcaggcga
     8041 caaccatacg ctgagagatc ctcactacgt agaagataaa ggccacaaat acttagtatt
     8101 tgaagcaaac actggaactg aagatggcta ccaaggcgaa gaatctttat ttaacaaagc
     8161 atactatggc aaaagcacat cattcttccg tcaagaaagt caaaaacttc tgcaaagcga
     8221 taaaaaacgc acggctgagt tagcaaacgg cgctctcggt atgattgagc taaacgatga
     8281 ttacacactg aaaaaagtga tgaaaccgct gattgcatct aacacagtaa cagatgaaat
     8341 tgaacgcgcg aacgtcttta aaatgaacgg caaatggtac ctgttcactg actcccgcgg
     8401 atcaaaaatg acgattgacg gcattacgtc taacgatatt tacatgcttg gttatgtttc
     8461 taattcttta actggcccat acaagccgct gaacaaaact ggccttgtgt taaaaatgga
     8521 tcttgatcct aacgatgtaa cctttactta ctcacacttc gctgtacctc aagcgaaagg
     8581 aaacaatgtc gtgattacaa gctatatgac aaacagagga ttctacgcag acaaacaatc
     8641 aacgtttgcg ccaagcttcc tgctgaacat caaaggcaag aaaacatctg ttgtcaaaga
     8701 cagcatcctt gaacaaggac aattaacagt taacaaataa gcggccgctg attagctaag
     8761 cagaaggcca tcctgacgga tgcctttttc gctttaatac tgcatgcact ctagaaatat
     8821 tggatcgtcg caccgggtta agcctggagt gcggtggtgc ctggtcggca ttttcgcagt
     8881 cgagggctct cgtgtagcct gggcgagttg ccgacgcagg cgaccctcct gccacggatc
     8941 cgcatggtac catcaggagg aatcacttcg caatgtctag attagataaa agtaaagtga
     9001 ttaacagcgc attagagctg cttaatgagg tcggaatcga aggtttaaca acccgtaaac
     9061 tcgcccagaa gcttggtgta gagcagccta cattgtattg gcatgtaaaa aataagcggg
     9121 ccctgctcga cgccttagcc attgagatgt tagataggca ccatactcac ttttgccctt
     9181 tagaagggga aagctggcaa gattttttac gtaataacgc taaaagtttt agatgtgctt
     9241 tactaagtca tcgcgatgga gcaaaagtac atttaggtac acggcctaca gaaaaacagt
     9301 atgaaactct cgaaaatcaa ttagcctttt tatgccaaca aggtttttca ctagagaacg
     9361 cgttatatgc actcagcgct gtggggcatt ttaccttagg ttgcgtattg gaagatcaag
     9421 agcatcaagt cgctaaagaa gaaagggaaa cacctactac tgatagtatg ccgccattat
     9481 tacgacaagc tatcgaatta tttgatcacc aaggtgcaga gccagccttc ttattcggcc
     9541 ttgaattgat catctgcgga ttagaaaaac aacttaaatg tgaaagtggg tcttaatgac
     9601 catgggctag atttaaatca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     9661 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     9721 gcggtggttt ttttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     9781 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     9841 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     9901 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     9961 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
    10021 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
    10081 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
    10141 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
    10201 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
    10261 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
    10321 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
    10381 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
    10441 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctaga
//
