LOCUS       sequence-210458-       10808 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of the phage Che9c RecET system for Recombineering in 
            mycobacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10808)
  AUTHORS   Murphy KC, Papavinasasundaram K, Sassetti CM
  TITLE     Mycobacterial recombineering.
  JOURNAL   Methods Mol Biol. 2015;1285:177-99. doi: 
            10.1007/978-1-4939-2450-9_10.
  PUBMED    25779316
REFERENCE   2  (bases 1 to 10808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2015"; volume: "1285:177-99. doi"; pages: " 
            10.1007/978-1-4939-2450-9_10"
COMMENT     Sequence Label: pNitET-SacB-kan
FEATURES             Location/Qualifiers
     source          1..10808
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      2247..2293
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2821..2860
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     terminator      complement(3980..4007)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4099..4185)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             4937..5752
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(join(6062..7396,7397..7483))
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     promoter        complement(7484..7929)
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     rep_origin      8050..8638
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8539..8558
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(10724..10771)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gcgaactccc ttatgcgggt ggcgcagaat gccaggaccc ttgtcattcc acgtcaattc
       61 atgcgccttt tcacctcgta ctgtcctgcc aaacacaagc aacggaggta cgcatatgga
      121 tatcgaattc aagcttttcg aagcttatcg atgtcgacgt agttacgaga tcggcggccg
      181 catatgagtg tgcccacaca ggacggaatg caccggttcg tcgacgagga cgtctaccac
      241 gctgaccggg gctcgctgtc ggtatccggc gcgaagctgc tgttgccgcc gtcgtgtccc
      301 gcgaaattcc gctgggagat ggacaacacc cggaagccga aaaaggtctg ggacttcgga
      361 catgtcgcgc acaaactggt gctcggcaag ggtgccgagt tcgagatcct cgaccccgag
      421 gtgcacgggc tgaaggcgga cggtacgccg tcggagaagc cgaccgcgac gggcatgtgg
      481 cgcaaggccg aggctgaggc tcgcaaacag ggcaaggtgc cgattcacgt cgacctgttc
      541 acgaaggcgt acgacatggc cgaaaaggtg cgtcagcacc cgacagccgg cccgatcttc
      601 gccaatcctg acggcgaggc cgaggtcgcg ctgtactaca ccgaccccga gaccggcgtg
      661 cggctgcgtg gccggatcga ctggctcact gacgatatcg atgattacaa gacgtcgatg
      721 accgcgaacc cggccgagct gaaaaccaag ttctacaagc tcggctattt catgcaggcg
      781 gcctggtaca tcgatctact ggtcgccctc gggctcgccg agaacccgcg attccggttc
      841 atcacgcagg agaaagaacc gccctacgtc gtgactccga tccagtacga cgacgaggcg
      901 atcgaagagg ggcggcgccg caaccgccag gcgatccggc tctacgccga ctgcatggaa
      961 tcgggcaagt ggcctgacta cagcgacgac gtggtcacga tcagcctgcc ctcgtggggg
     1021 ctgccgcgac cgcagaccgt cggcgacgtc gtcaccgaca gctatatcta cgacaccgac
     1081 ccgctcgaag aggccgaccc gattgaaggg gattacatct atggctgaaa atgctgtcac
     1141 caagcaggat tcgcccaagg cacccgagac gatctcgcaa gtgctgcagg tgctcgtgcc
     1201 gcagctggcg cgtgcagtgc ccaagggtat ggaccccgac cgcatcgcgc ggatcgtgca
     1261 aaccgagatc cgcaagtcgc gcaacgcgaa agctgctggc atcgctaagc agtccctcga
     1321 cgactgcacg caagagtcat ttgccggtgc gctgctgacc tcggccgcgc tcggtctcga
     1381 gcccggtgtc aacggcgagt gctacctcgt gccctaccgc gacacccggc gcggcgtggt
     1441 cgagtgccag ctgatcatcg gctaccaggg catcgtcaaa ctgttctggc agcacccgcg
     1501 cgcctcccgg atcgacgcgc agtgggtcgg cgcgaacgac gaattccatt acaccatggg
     1561 cctcaatccg acgctgaaac acgtgaaggc caagggtgat cggggtaatc cggtctactt
     1621 ctacgcgatc gtcgaggtga ccggcgctga gccgctgtgg gacgtgttca ccgccgacga
     1681 gatcagggaa ttgcgtcgcg gcaaggtcgg atcctcgggc gacatcaagg acccgcagcg
     1741 ctggatggag cggaagaccg cgctcaaaca ggtgctgaag ctggcaccga agacgacgcg
     1801 gctcgacgcg gcgatccgcg ccgacgatcg cccgggcacc gacctgtcac agtcgcaggc
     1861 gctcgcgctg ccgtcgaccg tcaagccgac ggccgactac atcgacggcg agatcgccga
     1921 gccgcacgag gtcgatacgc caccgaagtc gtcgcgcgca caacgcgcgc agcgcgccac
     1981 cgcgccggcg cccgacgtgc agatggccaa tcccgatcag ctgaagcgcc tcggcgagat
     2041 ccagaaggcc gaaaagtaca acgacgccga ttggttcaag ttcctcgccg attcggccgg
     2101 cgtcaaagcc acgcgcgccg ccgacctcac gttcgacgag gcgaaggctg tcatcgacat
     2161 gttcgacggg cccaacgcat gagcgccccg gcgaattccg acgcggtggt tgatctgcaa
     2221 ctagcgtacg atcgactgcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
     2281 cctttcgttt tatgccatca tggccgcggt gatcagctag ccacctgacg tcgggggggg
     2341 gggaaagcca cgttgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat
     2401 catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc
     2461 atattcaacg ggaaacgtct tgctcgaggc cgcgattaaa ttccaacatg gatgctgatt
     2521 tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgct
     2581 tgtatgggaa gccccatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca
     2641 atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga
     2701 ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg
     2761 ggaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat aaattaaacc
     2821 gatacaatta aaggctcctt ttggagcctt tttttttgga gcttaaggcg aactccctta
     2881 tgcgggtggc gcagaatgcc aggacccttg tcattccacg tcaattcatg cgccttttca
     2941 cctcgtactg tcctgccaaa cacaagcaac ggaggtacgg acatgaacac tttcttctcc
     3001 tcagaccagg tctcggcgcc cgatcgcgtc gcgctctggc acgatgtcat ctgccgtagc
     3061 tatgtcccgc tcaacatcac cctcacgagc gagcaaccct tcatcggtac ggtctcgacg
     3121 ggcaacttgg gcacggtacg tatcgcgacg tcctcgtcac tgccccaaca gatcacccgc
     3181 actcgtcgct tgatcaggca ggacgagcgt gagtacctca tggttggggt gcagtccgcc
     3241 ggccatgcac tcgtgcagca gcacggcaga actgcacgag tcggtcgcgg tggactggtc
     3301 ttctgggaca cccgccatcc ctacgacatc ctcttcccga cagactggag gatgagcgta
     3361 ttccagttcc cgcgatactc tttcggcttc accgaagact tcatcggcag gatgaccgcg
     3421 gtgaacgtcg ggggcgatcg cggtatcggc cgagtggttt catccttcat gacaagcatc
     3481 aacgatgcga ccgacgcagg agacttggcg gaggtagctt cactccacaa cagtgctgtc
     3541 gatcttctgt cagcggcgat acggaccgag cttgccgatc aagccgccgc ctccgacggc
     3601 ctactcgagt gtgtgctcgc gtatatccga cagaacctgg ccgacccgaa cctgtgtgcc
     3661 tcacagatcg cggcggaaca caacgtctct gtgcggaccc tccaccgact gttctcggcc
     3721 acgggacagg gcgtggccga acacatccgt aacctccgac tcgagcgcat caagactgag
     3781 ctggcagacc caacgagccg gcgatatacg atcagcgctt tggcgagaaa atgggggttc
     3841 ctcgatccct caacgttctc acgcgcgttc aaagacgcct acggcatcac tgcccgagag
     3901 tgggcggctt ctgcatcagc atcaccgacg gaggtttcgt agtctagata tgacgacagg
     3961 aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct taatttgatg
     4021 cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct tcgcaacgtt
     4081 caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat
     4141 aaaacgaaag gcccagcctt tcgactgagc ctttcgtttt atttgatgcc tggcagttcc
     4201 ctactctcgc atggggagac cccacactac catcggcgct agagccgtga acacagggcg
     4261 aacgccagcc cgccgacggc gggggttccg accgctgcaa ctcccggtgc aaccttgtcc
     4321 cggtctattc tcttcactgc accagctcca atctggtgtg aatgcccctc gtctgttcgc
     4381 gcaggcgggg ggctctattc gtttgtcagc atcgaaagta gccagatcag ggatgcgttg
     4441 caaccgcgta tgcccaggtc agaagagtcg cacaagagtt gcagacccct ggaaagaaaa
     4501 atggccagag ggcgaaaaca ccctctgacc agcggagcgg gcgacgggaa tcgaacccgc
     4561 gtagctagtt tggaagaatg ggtgtctgcc gaccacatat atgggccggt caagataggt
     4621 ttttaccccc tctcggctgc atcctctaag tggaaagaaa ttgcaggtcg tagaagcgcg
     4681 ttgaagcctg agagttgcac aggagttgca acccggtagc cttgttcacg acgagaggag
     4741 acctagttgg cacgtcgcgg atggggatcg ctgaagactc agcgcagcgg gaggatcagc
     4801 tagccacctg acgtcggggg ggggggaaag ccacgttgtg tctcaaaatc tctgatgtta
     4861 cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag
     4921 taatacaagg ggtgttatga gccatattca acgggaaacg tcttgctcga ggccgcgatt
     4981 aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca
     5041 atcaggtgcg acaatctatc gcttgtatgg gaagccccat gcgccagagt tgtttctgaa
     5101 acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct
     5161 gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg
     5221 gttactcacc actgcgatcc ccgggaaaac agcattccag gtattagaag aatatcctga
     5281 ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc
     5341 tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg
     5401 aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt
     5461 tgaacaagtc tggaaagaaa tgcataatct tttgccattc tcaccggatt cagtcgtcac
     5521 tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat
     5581 tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg
     5641 cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa
     5701 tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt
     5761 ggttaattgg ttgtaacact ggcagagcat tacgctgact tgacgggacg gcggctttgt
     5821 tgaataaatc gaacttttgc tgagttgaag gatcagatca cgcatcttcc cgacaacgca
     5881 gaccgttccg tggcaaagca aaagttcaaa atcaccaact ggtccaccta caacaaagct
     5941 ctcaccaacc gtggctccct cactttctgg ctggatgatg gggcgattca ggcctggtat
     6001 gagtcagcaa caccttcttc acgaggcaga cctcactaga tcggcatttt cttttgcgtt
     6061 tttatttgtt aactgttaat tgtccttgtt caaggatgct gtctttgaca acagatgttt
     6121 tcttgccttt gatgttcagc aggaagcttg gcgcaaacgt tgattgtttg tctgcgtaga
     6181 atcctctgtt tgtcatatag cttgtaatca cgacattgtt tcctttcgct tgaggtacag
     6241 cgaagtgtga gtaagtaaag gttacatcgt taggatcaag atccattttt aacacaaggc
     6301 cagttttgtt cagcggcttg tatgggccag ttaaagaatt agaaacataa ccaagcatgt
     6361 aaatatcgtt agacgtaatg ccgtcaatcg tcatttttga tccgcgggag tcagtgaaca
     6421 ggtaccattt gccgttcatt ttaaagacgt tcgcgcgttc aatttcatct gttactgtgt
     6481 tagatgcaat cagcggtttc atcacttttt tcagtgtgta atcatcgttt agctcaatca
     6541 taccgagagc gccgtttgct aactcagccg tgcgtttttt atcgctttgc agaagttttt
     6601 gactttcttg acggaagaat gatgtgcttt tgccatagta tgctttgtta aataaagatt
     6661 cttcgccttg gtagccatct tcagttccag tgtttgcttc aaatactaag tatttgtggc
     6721 ctttatcttc tacgtagtga ggatctctca gcgtatggtt gtcgcctgag ctgtagttgc
     6781 cttcatcgat gaactgctgt acattttgat acgtttttcc gtcaccgtca aagattgatt
     6841 tataatcctc tacaccgttg atgttcaaag agctgtctga tgctgatacg ttaacttgtg
     6901 cagttgtcag tgtttgtttg ccgtaatgtt taccggagaa atcagtgtag aataaacgga
     6961 tttttccgtc agatgtaaat gtggctgaac ctgaccattc ttgtgtttgg tcttttagga
     7021 tagaatcatt tgcatcgaat ttgtcgctgt ctttaaagac gcggccagcg tttttccagc
     7081 tgtcaataga agtttcgccg actttttgat agaacatgta aatcgatgtg tcatccgcat
     7141 ttttaggatc tccggctaat gcaaagacga tgtggtagcc gtgatagttt gcgacagtgc
     7201 cgtcagcgtt ttgtaatggc cagctgtccc aaacgtccag gccttttgca gaagagatat
     7261 ttttaattgt ggacgaatcg aattcaggaa cttgatattt ttcatttttt tgctgttcag
     7321 ggatttgcag catatcatgg cgtgtaatat gggaaatgcc gtatgtttcc ttatatggct
     7381 tttggttcgt ttctttcgca aacgcttgag ttgcgcctcc tgccagcagt gcggtagtaa
     7441 aggttaatac tgttgcttgt tttgcaaact ttttgatgtt catcgttcat gtctcctttt
     7501 ttatgtactg tgttagcggt ctgcttcttc cagccctcct gtttgaagat ggcaagttag
     7561 ttacgcacaa taaaaaaaga cctaaaatat gtaaggggtg acgccaaagt atacactttg
     7621 ccctttacac attttaggtc ttgcctgctt tatcagtaac aaacccgcgc gatttacttt
     7681 tcgacctcat tctattagac tctcgtttgg attgcaactg gtctattttc ctcttttgtt
     7741 tgatagaaaa tcataaaagg atttgcagac tacgggccta aagaactaaa aaatctatct
     7801 gtttcttttc attctctgta ttttttatag tttctgttgc atgggcataa agttgccttt
     7861 ttaatcacaa ttcagaaaat atcataatat ctcatttcac taaataatag tgaacggcag
     7921 gtatatgtga tgggttaaaa aggatcgatc ctctagagtc gacctgcagc tggatccgca
     7981 attgtcttgg ccattgcgaa gtctagttcc atgagcgtca gaccccgtag aaaagatcaa
     8041 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     8101 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     8161 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     8221 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     8281 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     8341 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     8401 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     8461 tcccgagggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     8521 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     8581 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     8641 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     8701 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     8761 taccgctcgc cgcagccgaa cgaccgagcg caacgcgtga gcccaccagc tccgtaagtt
     8821 cgggcgctgt gtggctcgta cccgcgcatt caggcggcag ggggtctaac gggtctaagg
     8881 cggcgtgtac ggccgccaca gcggctctca gcggcccgga aacgtcctcg aaacgacgca
     8941 tgtgttcctc ctggttggta caggtggttg ggggtgctcg gctgtcgctg gtgttccacc
     9001 accagggctc gacgggagag cgggggagtg tgcagttgtg gggtggcccc tcagcgaaat
     9061 atctgacttg gagctcgtgt cggaccatac accggtgatt aatcgtggtc tactaccaag
     9121 cgtgagccac gtcgccgacg aatttgagca gctctggctg ccgtactggc cgctggcaag
     9181 cgacgatctg ctcgagggga tctaccgcca aagccgcgcg tcggccctag gccgccggta
     9241 catcgaggcg aacccaacag cgctggcaaa cctgctggtc gtggacgtag accatccaga
     9301 cgcagcgctc cgagcgctca gcgcccgggg gtcccatccg ctgcccaacg cgatcgtggg
     9361 caatcgcgcc aacggccacg cacacgcagt gtgggcactc aacgcccctg ttccacgcac
     9421 cgaatacgcg cggcgtaagc cgctcgcata catggcggcg tgcgccgaag gccttcggcg
     9481 cgccgtcgat ggcgaccgca gttactcagg cctcatgacc aaaaaccccg gccacatcgc
     9541 ctgggaaacg gaatggctcc actcagatct ctacacactc agccacatcg aggccgagct
     9601 cggcgcgaac atgccaccgc cgcgctggcg tcagcagacc acgtacaaag cggctccgac
     9661 gccgctaggg cggaattgcg cactgttcga ttccgtcagg ttgtgggcct atcgtcccgc
     9721 cctcatgcgg atctacctgc cgacccggaa cgtggacgga ctcggccgcg cgatctatgc
     9781 cgagtgccac gcgcgaaacg ccgaatttcc gtgcaacgac gtgtgtcccg gaccgctacc
     9841 ggacagcgag gtccgcgcca tcgccaacag catttggcgt tggatcacaa ccaagtcgcg
     9901 catttgggcg gacgggatcg tggtctacga ggccacactc agtgcgcgcc agtcggccat
     9961 ctcgcggaag ggcgcagcag cgcgcacggc ggcgagcaca gttgcgcggc gcgcaaagtc
    10021 cgcgtcagcc atggaggcat tgctatgagc gacggctaca gcgacggcta caaccggcag
    10081 ccgactgtcc gcaaaaagcg gcgcgtgacc gccgccgaag gcgctcgaat caccggacta
    10141 tccgaacgcc acgtcgtccg gctcgtggcg caggaacgca gcgagtggct cgccgagcag
    10201 gctgcacgcc gcgaacgcat ccgcgcctat cacgacgacg agggccactc ttggccgcaa
    10261 acggccaaac atttcgggct gcatctggac accgttaagc gactcggcta tcgggcgagg
    10321 aaagagcgtg cggcagaaca ggaagcggct caaaaggccc acaacgaagc cgacaatcca
    10381 ccgctgttct aacgcaattg gggagcgggt gtcgcggggg ttccgtgggg ggttccgttg
    10441 caacgggtcg gacaggtaaa agtcctggta gacgctagtt ttctggtttg ggccatgcct
    10501 gtctcgttgc gtgtttcgtt gcgcccgttt tgaataccag ccagacgaga cggggttcta
    10561 cgaatcttgg tcgataccaa gccatttccg ctgaatatcg gggagctcac cgccagaatc
    10621 ggtggttgtg gtgatgtacg tggcgaactc cgttgtagtg cctgtggtgg catccgtggc
    10681 gcggccgcgg taccagatct ccggatatag ttcctccttt cagcaaaaaa cccctcaaga
    10741 cccgtttaga ggccccaagg ggttatgcta gttattgctc agttaactac gtcgacatcg
    10801 atagtact
//
