LOCUS       sequence-210491-        1779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cyan color bright luminescent calcium indicator with intermediate 
            affinity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Hossain MN, Suzuki K, Iwano M, Matsuda T, Nagai T
  TITLE     Bioluminescent Low-Affinity Ca(2+) Indicator for ER with Multicolor 
            Calcium Imaging in Single Living Cells.
  JOURNAL   ACS Chem Biol. 2018 Mar 9. doi: 10.1021/acschembio.7b01014.
  PUBMED    29494125
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2018 Mar 9. doi: 10.1021/acschembio.7b01014."
COMMENT     Sequence Label: CeNL(Ca2+)_19u-pcDNA3
FEATURES             Location/Qualifiers
     source          1..1779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..3,4..6,7..717)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(46..67)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(307..326)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     654..675
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1378..1455
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccctgtcctg gggcgtgcag tgcttcgccc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaactactt tagcgacaac gtctatatca ccgccgacaa gcagaagaac
      481 ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcggcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagca cccagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagggt
      721 accgtcttca cactcgaaga tttcgttggg gactggcgac agacagccgg ctacaacctg
      781 gaccaagtcc ttgaacaggg aggtgtgtcc agtttgtttc agaatctcgg ggtgtccgta
      841 actccgatcc aaaggattgt cctgagcggt gaaaatgggc tgaagatcga catccatgtc
      901 atcatcccgt atgaaggtcc atggatgcat gaccaactga cagaagagca gattgcagag
      961 ttcaaagaag ccttctcatt attcgacaag gatggggacg gcaccatcac cacaaaggaa
     1021 cttggcaccg ttatgaggtc gcttggacaa aacccaacgg aagcagaatt gcaggatatg
     1081 atcaatgaag tcgatgctga tggcaatgga acgatttact ttcctgattt tcttactatg
     1141 atggctagaa aaatgaagga cacagacagc gaagaggaaa tccgagaagc attccgtgtt
     1201 tgggacaagg atgggaacgg ctacatcagc gctgctgatt tacgtcacgt catgacaaac
     1261 ctcggggaga agttaacaga tgaagaagtt gatgaaatga taagggaagc agatatcgat
     1321 ggtgaaggcc aagtaaacta tgaagagttt gtacaaatga tgacagcaaa gggggggaag
     1381 aggcgctgga agaaaaactt cattgccgtc agcgctgcca accggttcaa gaagatctcc
     1441 agctccgggg cactggagct cctgagcggc gaccaaatgg gccagatcga aaaaattttt
     1501 aaggtggtgt accctgtgga tgatcatcac tttaaggtga tcctgcacta tggcacactg
     1561 gtaatcgacg gggttacgcc gaacatgatc gactatttcg gacggccgta tgaaggcatc
     1621 gccgtgttcg acggcaaaaa gatcactgta acagggaccc tgtggaacgg caacaaaatt
     1681 atcgacgagc gcctgatcaa ccccgacggc tccctgctgt tccgagtaac catcaacgga
     1741 gtgaccggct ggcggctgtg cgaacgcatt ctggcgtaa
//
