LOCUS       sequence-210497-        6315 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent Src.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6315)
  AUTHORS   Mainou BA, Dermody TS
  TITLE     Src kinase mediates productive endocytic sorting of reovirus during 
            cell entry.
  JOURNAL   J Virol. 2011 Apr;85(7):3203-13. doi: 10.1128/JVI.02056-10. Epub 
            2011 Jan 19.
  PUBMED    21248042
REFERENCE   2  (bases 1 to 6315)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JVI.02056-10"; journalName: "J Virol"; date: "2011-04"; 
            volume: "85"; issue: "7"; pages: "3203-13"
COMMENT     Sequence Label: Src-EGFP
FEATURES             Location/Qualifiers
     source          1..6315
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2195..2204
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2201..2203,2204..2206,2207..2920)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2201..2203,2204..2206,2207..2917)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2246..2267)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2507..2526)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2854..2875
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    3043..3164
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3080..3099)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3134..3153
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3171..3626)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3308..3329)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3520..3539
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3653..3757
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3759..4116
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3759..3950
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3782..3802)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3967..4102
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4151..4945
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4151..4945
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4205..4224)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4815..4834
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5133..5152)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5177..5224
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5553..6141
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6042..6061
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggactcag atctcgagct caagcttcga attcatgggc agcaacaaga gcaagcccaa
      601 ggacgccagc cagcggcgcc gcagcctgga gccctcggaa aacgtgcacg gggcaggggg
      661 cgccttcccg gcctcacaga caccgagcaa gcccgcctcc gccgacggcc accgcgggcc
      721 cagcgccgcc ttcgtgccgc ccgcggccga gcccaagctc ttcggaggct tcaactcctc
      781 ggacaccgtc acctccccgc agagggcggg gcctctggca ggtggggtga ccacctttgt
      841 ggccctctat gactatgagt cacggacaga gactgacctg tccttcaaga aaggggagcg
      901 gctgcagatt gttaacaaca cggagggaga ctggtggctg gcacactcgc tgagcacggg
      961 acagaccggt tacatcccca gcaactatgt ggcgccctcc gactccatcc aggctgagga
     1021 gtggtacttt ggcaagatca ctagacggga atcagagcgg ctgctgctca acgccgagaa
     1081 cccgagaggg accttcctcg tgagggagag tgagaccaca aaaggtgcct actgcctctc
     1141 tgtatccgac ttcgacaatg ccaagggcct aaatgtgaaa cactacaaga tccgcaagct
     1201 ggacagcggc ggtttctaca tcacctcccg cacccagttc aacagcctgc agcagctcgt
     1261 ggcttactac tccaaacatg ctgatggcct gtgtcaccgc ctcactaccg tatgtcccac
     1321 atccaagcct cagacccagg gattggccaa ggatgcgtgg gagatccccc gggagtccct
     1381 gcggctggag gtcaagctgg gccagggttg cttcggagag gtgtggatgg ggacctggaa
     1441 cggcaccacg agggttgcca tcaaaactct gaagccaggc accatgtccc cagaggcctt
     1501 cctgcaggag gcccaagtca tgaagaaact gaggcacgag aaactggtgc agctgtatgc
     1561 tgtggtgtcg gaagaaccca tttacattgt gacagagtac atgaacaagg ggagtctgct
     1621 ggactttctc aagggggaaa cgggcaaata tttgcggcta ccccagctgg tggacatgtc
     1681 tgctcagatc gcttcaggca tggcctatgt ggagcggatg aactatgtgc accgggacct
     1741 tcgagccgcc aatatcctag taggggagaa cctggtgtgc aaagtggccg actttgggtt
     1801 ggcccggctc atagaagaca acgaatacac agcccggcaa ggtgccaaat tccccatcaa
     1861 gtggaccgcc cctgaagctg ctctgtacgg caggttcacc atcaagtcgg atgtgtggtc
     1921 ctttgggatt ctgctgaccg agctcaccac taagggaaga gtgccctatc ctgggatggt
     1981 gaaccgtgag gttctggacc aggtggagcg gggctaccgg atgccttgtc cccccgagtg
     2041 ccccgagtcc ctgcatgacc ttatgtgcca gtgctggcgg aaggagcccg aggagcggcc
     2101 caccttcgag tacctgcagg ccttcctgga agactacttt acgtccactg agccacagta
     2161 ccagcccggg gagaacctaa gggatccacc ggtcgccacc atggtgagca agggcgagga
     2221 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     2281 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     2341 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     2401 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     2461 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     2521 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     2581 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     2641 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     2701 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     2761 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     2821 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     2881 cgccgggatc actctcggca tggacgagct gtacaagtaa agcggccgcg actctagatc
     2941 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
     3001 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
     3061 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     3121 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaaggcgt aaattgtaag
     3181 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
     3241 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
     3301 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
     3361 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
     3421 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
     3481 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     3541 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     3601 gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac
     3661 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     3721 ctgataaatg cttcaataat attgaaaaag gaagagtcct gaggcggaaa gaaccagctg
     3781 tggaatgtgt gtcagttagg gtgtggaaag tccctaggct ccccagcagg cagaagtatg
     3841 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     3901 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     3961 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     4021 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     4081 tgaggaggct tttttggagg cctaggcttt tgcaaagatc gatcaagaga caggatgagg
     4141 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     4201 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     4261 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     4321 gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     4381 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     4441 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     4501 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     4561 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     4621 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag
     4681 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     4741 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     4801 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     4861 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     4921 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg
     4981 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     5041 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     5101 gagttcttcg cccaccctag ggggaggcta actgaaacac ggaaggagac aataccggaa
     5161 ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt
     5221 gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc
     5281 cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt
     5341 gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc ctcaggttac
     5401 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     5461 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     5521 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     5581 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     5641 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     5701 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     5761 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     5821 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     5881 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     5941 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     6001 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     6061 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     6121 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     6181 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     6241 attaccgcca tgcattagtt attaatagta atcaattacg gggtcattag ttcatagccc
     6301 atatatggag ttccg
//
