LOCUS       sequence-210527-       11342 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of HA-YWAA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11342)
  AUTHORS   Lu G, Middleton RE, Sun H, Naniong M, Ott CJ, Mitsiades CS, Wong KK,
            Bradner JE, Kaelin WG Jr
  TITLE     The myeloma drug lenalidomide promotes the cereblon-dependent 
            destruction of Ikaros proteins.
  JOURNAL   Science. 2014 Jan 17;343(6168):305-9. doi: 10.1126/science.1244917. 
            Epub 2013 Nov 29.
  PUBMED    24292623
REFERENCE   2  (bases 1 to 11342)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1244917"; journalName: "Science"; date: 
            "2014-01-17- 17"; volume: "343"; issue: "6168"; pages: "305-9"
COMMENT     Sequence Label: plenti-UbcP-HA-YWAA-pGK-HYG
FEATURES             Location/Qualifiers
     source          1..11342
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    124..241
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        333..1544
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1381..1400
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             1599..1625
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    1647..1671
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2998..3022)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        3166..3665
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3201..3224)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3598..3617
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3683..4708
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             3683..4708
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(4739..4772)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    4828..5416
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4881..4901)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5299..5310)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5419..5435)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5420..5436)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5625..5858
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5930..6051
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5967..5986)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6021..6040
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6091..6226
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6246..6265)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6247..6265)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(6288..6646)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6328..6349)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6540..6559
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6673..6777
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6779..7136
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6779..6970
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6802..6822)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6987..7122
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7171..7965
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7171..7965
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7225..7244)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7835..7854
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8153..8172)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8197..8244
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8573..9161
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9062..9081
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        9336..9639
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9641..9839
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9794..9814
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             9857..10037
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10084..10209
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10706..10939
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11124..11168
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag atccattcga
       61 ttagtgaacg gatcggcact gcgtgcgcca attctgcaga caaatggcag tattcatcca
      121 caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
      181 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
      241 tcgggtttat tacagggaca gcagagatcc agtttggtta gtaccgggcc cgctctagcc
      301 ctagatttaa cccaacagaa ggctcgacgc gtggcctccg cgccgggttt tggcgcctcc
      361 cgcgggcgcc cccctcctca cggcgagcgc tgccacgtca gacgaagggc gcagcgagcg
      421 tcctgatcct tccgcccgga cgctcaggac agcggcccgc tgctcataag actcggcctt
      481 agaaccccag tatcagcaga aggacatttt aggacgggac ttgggtgact ctagggcact
      541 ggttttcttt ccagagagcg gaacaggcga ggaaaagtag tcccttctcg gcgattctgc
      601 ggagggatct ccgtggggcg gtgaacgccg atgattatat aaggacgcgc cgggtgtggc
      661 acagctagtt ccgtcgcagc cgggatttgg gtcgcggttc ttgtttgtgg atcgctgtga
      721 tcgtcacttg gtgagtagcg ggctgctggg ctggccgggg ctttcgtggc cgccgggccg
      781 ctcggtggga cggaagcgtg tggagagacc gccaagggct gtagtctggg tccgcgagca
      841 aggttgccct gaactggggg ttggggggag cgcagcaaaa tggcggctgt tcccgagtct
      901 tgaatggaag acgcttgtga ggcgggctgt gaggtcgttg aaacaaggtg gggggcatgg
      961 tgggcggcaa gaacccaagg tcttgaggcc ttcgctaatg cgggaaagct cttattcggg
     1021 tgagatgggc tggggcacca tctggggacc ctgacgtgaa gtttgtcact gactggagaa
     1081 ctcggtttgt cgtctgttgc gggggcggca gttatggcgg tgccgttggg cagtgcaccc
     1141 gtacctttgg gagcgcgcgc cctcgtcgtg tcgtgacgtc acccgttctg ttggcttata
     1201 atgcagggtg gggccacctg ccggtaggtg tgcggtaggc ttttctccgt cgcaggacgc
     1261 agggttcggg cctagggtag gctctcctga atcgacaggc gccggacctc tggtgagggg
     1321 agggataagt gaggcgtcag tttctttggt cggttttatg tacctatctt cttaagtagc
     1381 tgaagctccg gttttgaact atgcgctcgg ggttggcgag tgtgttttgt gaagtttttt
     1441 aggcaccttt tgaaatgtaa tcatttgggt caatatgtaa ttttcagtgt tagactagta
     1501 aattgtccgc taaattctgg ccgtttttgg cttttttgtt agacgctaga ctgccggatc
     1561 gaattgtcga cgaattcgga tccagatctg ccaccatgta cccatacgat gttccagatt
     1621 acgctggcgg agctcgaggt tatcaaacaa gtttgtacaa aaaagttggc atggccggcg
     1681 aaggagatca gcaggacgct gcgcacaaca tgggcaacca cctgccgctc ctgcctgaga
     1741 gtgaggaaga agatgaaatg gaagttgaag accaggatag taaagaagcc aaaaaaccaa
     1801 acatcataaa ttttgacacc agtctgccga catcacatac atacctaggt gctgatatgg
     1861 aagaatttca tggcaggact ttgcacgatg acgacagctg tcaggtgatt ccagttcttc
     1921 cacaagtgat gatgatcctg attcccggac agacattacc tcttcagctt tttcaccctc
     1981 aagaagtcag tatggtgcgg aatttaattc agaaagatag aacctttgct gttcttgcat
     2041 acagcaatgt acaggaaagg gaagcacagt ttggaacaac agcagagata tatgcctatc
     2101 gagaagaaca ggattttgga attgagatag tgaaagtgaa agcaattgga agacaaaggt
     2161 tcaaagtcct tgagctaaga acacagtcag atggaatcca gcaagctaaa gtgcaaattc
     2221 ttcccgaatg tgtgttgcct tcaaccatgt ctgcagttca attagaatcc ctcaataagt
     2281 gccagatatt tccttcaaaa cctgtctcaa gagaagacca atgttcatat aaatggtggc
     2341 agaaatacca gaagagaaag tttcattgtg caaatctaac ttcatggcct cgctggctgt
     2401 attccttata tgatgctgag accttaatgg acagaatcaa gaaacagcta cgtgaatggg
     2461 atgaaaatct aaaagatgat tctcttcctt caaatccaat agatttttct tacagagtag
     2521 ctgcttgtct tcctattgat gatgtattga gaattcagct ccttaaaatt ggcagtgcta
     2581 tccagcgact tcgctgtgaa ttagacatta tgaataaatg tacttccctt tgctgtaaac
     2641 aatgtcaaga aacagaaata acaaccaaaa atgaaatatt cagtttatcc ttatgtgggc
     2701 cgatggcagc ttatgtgaat cctcatggat atgtgcatga gacacttact gtgtataagg
     2761 cttgcaactt gaatctgata ggccggcctt ctacagaaca cagctggttt cctggggctg
     2821 ccgcgactgt tgcccagtgt aagatctgtg caagccatat tggatggaag tttacggcca
     2881 ccaaaaaaga catgtcacct caaaaatttt ggggcttaac gcgatctgct ctgttgccca
     2941 cgatcccaga cactgaagat gaaataagtc cagacaaagt aatactttgc ttgtaagacc
     3001 cagctttctt gtacaaagtg gttcgataac gaattccgcc ccccccccct aacgttactg
     3061 gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat
     3121 tgccgtcttt tggcaatgtg agggccgcgt ttaacgaatt ctaccgggta ggggaggcgc
     3181 ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca
     3241 caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt
     3301 tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg
     3361 ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg
     3421 tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat
     3481 agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg
     3541 ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat
     3601 tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc
     3661 tttcgacctg catcccgcca ccatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa
     3721 gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga
     3781 atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg
     3841 cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc
     3901 gattccggaa gtgcttgaca ttggggaatt cagcgagagc ctgacctatt gcatctcccg
     3961 ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctgca
     4021 gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt
     4081 cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc
     4141 gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc
     4201 cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca
     4261 cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc
     4321 ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt
     4381 cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca
     4441 tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg gtcttgacca
     4501 actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg
     4561 cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag
     4621 cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc
     4681 cagcactcgt ccgagggcaa aggaatagtg tacaagtagg aattcgtcga gggacctaat
     4741 aacttcgtat agcatacatt atacgaagtt atacatgttt aagggttccg gttccactag
     4801 gtacaattcg atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga
     4861 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     4921 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     4981 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     5041 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     5101 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     5161 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     5221 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     5281 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     5341 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     5401 tgggccgcct ccccgcatcg ataccgtcga cctcgatcga gacctagaaa aacatggagc
     5461 aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga
     5521 ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa
     5581 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     5641 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     5701 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     5761 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     5821 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     5881 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     5941 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     6001 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     6061 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     6121 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     6181 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca
     6241 attcgcccta tagtgagtcg tattagcatg tgagcaaaag gccagcataa atcaaaagaa
     6301 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     6361 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     6421 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
     6481 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     6541 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
     6601 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt
     6661 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     6721 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtcct
     6781 gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     6841 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     6901 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     6961 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     7021 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     7081 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaagatc
     7141 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
     7201 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     7261 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     7321 accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg
     7381 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     7441 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     7501 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     7561 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     7621 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     7681 ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     7741 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     7801 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     7861 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     7921 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
     7981 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg
     8041 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     8101 agcgcgggga tctcatgctg gagttcttcg cccaccctag ggggaggcta actgaaacac
     8161 ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa
     8221 cgcacggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg
     8281 tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac
     8341 cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc
     8401 ctgccatagc ctcaggttac tcatatatac tttagattga tttaaaactt catttttaat
     8461 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     8521 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     8581 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     8641 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     8701 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     8761 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     8821 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     8881 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     8941 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     9001 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     9061 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     9121 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     9181 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     9241 ctgattctgt ggataaccgt attaccgcca tgcattagtt attaatagta atcaattacg
     9301 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     9361 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     9421 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     9481 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     9541 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     9601 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     9661 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     9721 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     9781 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcagc
     9841 gcgttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     9901 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     9961 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
    10021 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
    10081 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
    10141 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
    10201 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
    10261 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
    10321 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
    10381 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
    10441 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
    10501 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cggccgctga
    10561 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
    10621 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
    10681 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
    10741 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
    10801 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
    10861 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
    10921 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
    10981 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
    11041 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
    11101 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
    11161 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
    11221 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
    11281 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
    11341 cc
//
