LOCUS       sequence-21053-e         604 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 604)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG423GPD-ccdB-Cerulean
FEATURES             Location/Qualifiers
     source          1..604
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(17..141)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(149..165)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(149..165)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(171..434)
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     complement(254..277)
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
ORIGIN
        1 cctaataggc ggccgccata gtgactggat atgttgtgtt ttacagtatt atgtagtctg
       61 ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc
      121 agcttttttg tacaaacttg tgatggggga tccactagtt ctagaatccg tcgaaactaa
      181 gttctggtgt tttaaaacta aaaaaaagac taactataaa agtagaattt aagaagttta
      241 agaaatagat ttacagaatt acaatcaata cctaccgtct ttatatactt attagtcaag
      301 taggggaata atttcaggga actggtttca accttttttt tcagcttttt ccaaatcaga
      361 gagagcagaa ggtaatagaa ggtgtaagaa aatgagatag atacatgcgt gggtcaattg
      421 ccttgtgtca tcatttactc caggcaggtt gcatcactcc attgaggttg tgcccgtttt
      481 ttgcctgttt gtgcccctgt tctctgtagt tgcgctaaaa gaatggacct atgaactgat
      541 ggttggtgaa gaaaacaata ttttggtgct gggattcttt ttttttctgg atgccagctt
      601 aaaa
//
