LOCUS       sequence-210530-       11339 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of FLAG-CRBN.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11339)
  AUTHORS   Lu G, Middleton RE, Sun H, Naniong M, Ott CJ, Mitsiades CS, Wong KK,
            Bradner JE, Kaelin WG Jr
  TITLE     The myeloma drug lenalidomide promotes the cereblon-dependent 
            destruction of Ikaros proteins.
  JOURNAL   Science. 2014 Jan 17;343(6168):305-9. doi: 10.1126/science.1244917. 
            Epub 2013 Nov 29.
  PUBMED    24292623
REFERENCE   2  (bases 1 to 11339)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1244917"; journalName: "Science"; date: 
            "2014-01-17- 17"; volume: "343"; issue: "6168"; pages: "305-9"
COMMENT     Sequence Label: plenti-UbcP-FLAG-CRBN-pGK-HYG
FEATURES             Location/Qualifiers
     source          1..11339
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    251..368
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        460..1671
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1508..1527
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      1717..1726
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1726..1749
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    1771..1795
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3122..3146)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        3290..3789
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3325..3348)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3722..3741
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3807..4832
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             3807..4832
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(4863..4896)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    4952..5540
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5005..5025)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5423..5434)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5543..5559)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5544..5560)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5749..5982
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6054..6175
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6091..6110)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6145..6164
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6215..6350
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6370..6389)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6371..6389)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(6412..6770)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6452..6473)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6664..6683
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6797..6901
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6903..7260
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6903..7094
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6926..6946)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7111..7246
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7295..8089
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7295..8089
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7349..7368)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7959..7978
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8277..8296)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8321..8368
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8697..9285
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9186..9205
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        9460..9763
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9765..9963
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9918..9938
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             9981..10161
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10208..10333
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10830..11063
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11248..11292
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
       61 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
      121 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc
      181 cattcgatta gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa atggcagtat
      241 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
      301 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
      361 aaaattttcg ggtttattac agggacagca gagatccagt ttggttagta ccgggcccgc
      421 tctagcccta gatttaaccc aacagaaggc tcgacgcgtg gcctccgcgc cgggttttgg
      481 cgcctcccgc gggcgccccc ctcctcacgg cgagcgctgc cacgtcagac gaagggcgca
      541 gcgagcgtcc tgatccttcc gcccggacgc tcaggacagc ggcccgctgc tcataagact
      601 cggccttaga accccagtat cagcagaagg acattttagg acgggacttg ggtgactcta
      661 gggcactggt tttctttcca gagagcggaa caggcgagga aaagtagtcc cttctcggcg
      721 attctgcgga gggatctccg tggggcggtg aacgccgatg attatataag gacgcgccgg
      781 gtgtggcaca gctagttccg tcgcagccgg gatttgggtc gcggttcttg tttgtggatc
      841 gctgtgatcg tcacttggtg agtagcgggc tgctgggctg gccggggctt tcgtggccgc
      901 cgggccgctc ggtgggacgg aagcgtgtgg agagaccgcc aagggctgta gtctgggtcc
      961 gcgagcaagg ttgccctgaa ctgggggttg gggggagcgc agcaaaatgg cggctgttcc
     1021 cgagtcttga atggaagacg cttgtgaggc gggctgtgag gtcgttgaaa caaggtgggg
     1081 ggcatggtgg gcggcaagaa cccaaggtct tgaggccttc gctaatgcgg gaaagctctt
     1141 attcgggtga gatgggctgg ggcaccatct ggggaccctg acgtgaagtt tgtcactgac
     1201 tggagaactc ggtttgtcgt ctgttgcggg ggcggcagtt atggcggtgc cgttgggcag
     1261 tgcacccgta cctttgggag cgcgcgccct cgtcgtgtcg tgacgtcacc cgttctgttg
     1321 gcttataatg cagggtgggg ccacctgccg gtaggtgtgc ggtaggcttt tctccgtcgc
     1381 aggacgcagg gttcgggcct agggtaggct ctcctgaatc gacaggcgcc ggacctctgg
     1441 tgaggggagg gataagtgag gcgtcagttt ctttggtcgg ttttatgtac ctatcttctt
     1501 aagtagctga agctccggtt ttgaactatg cgctcggggt tggcgagtgt gttttgtgaa
     1561 gttttttagg caccttttga aatgtaatca tttgggtcaa tatgtaattt tcagtgttag
     1621 actagtaaat tgtccgctaa attctggccg tttttggctt ttttgttaga cgctagactg
     1681 ccggatcgaa ttgtcgacga attcggatcc agatctgcca ccatggacta caaggacgac
     1741 gatgacaagg gcggagctcg aggttatcaa acaagtttgt acaaaaaagt tggcatggcc
     1801 ggcgaaggag atcagcagga cgctgcgcac aacatgggca accacctgcc gctcctgcct
     1861 gagagtgagg aagaagatga aatggaagtt gaagaccagg atagtaaaga agccaaaaaa
     1921 ccaaacatca taaattttga caccagtctg ccgacatcac atacatacct aggtgctgat
     1981 atggaagaat ttcatggcag gactttgcac gatgacgaca gctgtcaggt gattccagtt
     2041 cttccacaag tgatgatgat cctgattccc ggacagacat tacctcttca gctttttcac
     2101 cctcaagaag tcagtatggt gcggaattta attcagaaag atagaacctt tgctgttctt
     2161 gcatacagca atgtacagga aagggaagca cagtttggaa caacagcaga gatatatgcc
     2221 tatcgagaag aacaggattt tggaattgag atagtgaaag tgaaagcaat tggaagacaa
     2281 aggttcaaag tccttgagct aagaacacag tcagatggaa tccagcaagc taaagtgcaa
     2341 attcttcccg aatgtgtgtt gccttcaacc atgtctgcag ttcaattaga atccctcaat
     2401 aagtgccaga tatttccttc aaaacctgtc tcaagagaag accaatgttc atataaatgg
     2461 tggcagaaat accagaagag aaagtttcat tgtgcaaatc taacttcatg gcctcgctgg
     2521 ctgtattcct tatatgatgc tgagacctta atggacagaa tcaagaaaca gctacgtgaa
     2581 tgggatgaaa atctaaaaga tgattctctt ccttcaaatc caatagattt ttcttacaga
     2641 gtagctgctt gtcttcctat tgatgatgta ttgagaattc agctccttaa aattggcagt
     2701 gctatccagc gacttcgctg tgaattagac attatgaata aatgtacttc cctttgctgt
     2761 aaacaatgtc aagaaacaga aataacaacc aaaaatgaaa tattcagttt atccttatgt
     2821 gggccgatgg cagcttatgt gaatcctcat ggatatgtgc atgagacact tactgtgtat
     2881 aaggcttgca acttgaatct gataggccgg ccttctacag aacacagctg gtttcctggg
     2941 tatgcctgga ctgttgccca gtgtaagatc tgtgcaagcc atattggatg gaagtttacg
     3001 gccaccaaaa aagacatgtc acctcaaaaa ttttggggct taacgcgatc tgctctgttg
     3061 cccacgatcc cagacactga agatgaaata agtccagaca aagtaatact ttgcttgtaa
     3121 gacccagctt tcttgtacaa agtggttcga taacgaattc cgcccccccc ccctaacgtt
     3181 actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc
     3241 atattgccgt cttttggcaa tgtgagggcc gcgtttaacg aattctaccg ggtaggggag
     3301 gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc
     3361 tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct
     3421 ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc
     3481 cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt
     3541 ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc
     3601 caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg
     3661 ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg
     3721 gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg
     3781 ggcctttcga cctgcatccc gccaccatga aaaagcctga actcaccgcg acgtctgtcg
     3841 agaagtttct gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg
     3901 aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata
     3961 gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc
     4021 tcccgattcc ggaagtgctt gacattgggg aattcagcga gagcctgacc tattgcatct
     4081 cccgccgtgc acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc
     4141 tgcagccggt cgcggaggcc atggatgcga tcgctgcggc cgatcttagc cagacgagcg
     4201 ggttcggccc attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat
     4261 gcgcgattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg
     4321 cgtccgtcgc gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc
     4381 ggcacctcgt gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa
     4441 cagcggtcat tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca
     4501 tcttcttctg gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga
     4561 ggcatccgga gcttgcagga tcgccgcggc tccgggcgta tatgctccgc attggtcttg
     4621 accaactcta tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc
     4681 gatgcgacgc aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca
     4741 gaagcgcggc cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac
     4801 gccccagcac tcgtccgagg gcaaaggaat agtgtacaag taggaattcg tcgagggacc
     4861 taataacttc gtatagcata cattatacga agttatacat gtttaagggt tccggttcca
     4921 ctaggtacaa ttcgatatca agcttatcga taatcaacct ctggattaca aaatttgtga
     4981 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     5041 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     5101 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     5161 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     5221 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     5281 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     5341 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     5401 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     5461 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     5521 cctttgggcc gcctccccgc atcgataccg tcgacctcga tcgagaccta gaaaaacatg
     5581 gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac
     5641 aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt
     5701 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     5761 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     5821 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     5881 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     5941 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     6001 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     6061 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     6121 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     6181 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     6241 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     6301 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
     6361 cccaattcgc cctatagtga gtcgtattag catgtgagca aaaggccagc ataaatcaaa
     6421 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     6481 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     6541 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     6601 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     6661 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     6721 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac
     6781 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     6841 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     6901 tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     6961 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     7021 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     7081 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     7141 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     7201 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     7261 gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
     7321 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     7381 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     7441 caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg
     7501 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     7561 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     7621 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     7681 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     7741 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     7801 actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg
     7861 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     7921 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     7981 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     8041 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
     8101 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc
     8161 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     8221 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ctagggggag gctaactgaa
     8281 acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat
     8341 aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac
     8401 tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc
     8461 ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca
     8521 ggccctgcca tagcctcagg ttactcatat atactttaga ttgatttaaa acttcatttt
     8581 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     8641 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     8701 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     8761 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     8821 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     8881 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     8941 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     9001 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     9061 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     9121 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     9181 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     9241 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     9301 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     9361 tcccctgatt ctgtggataa ccgtattacc gccatgcatt agttattaat agtaatcaat
     9421 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
     9481 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     9541 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     9601 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     9661 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     9721 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
     9781 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
     9841 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
     9901 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
     9961 cagcgcgttt tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct
    10021 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
    10081 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
    10141 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
    10201 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
    10261 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
    10321 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
    10381 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
    10441 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
    10501 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
    10561 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
    10621 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccggccg
    10681 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
    10741 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
    10801 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
    10861 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
    10921 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
    10981 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
    11041 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
    11101 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
    11161 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
    11221 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
    11281 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattatt
//
