LOCUS       sequence-210540-       11339 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of FLAG-YWAA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11339)
  AUTHORS   Lu G, Middleton RE, Sun H, Naniong M, Ott CJ, Mitsiades CS, Wong KK,
            Bradner JE, Kaelin WG Jr
  TITLE     The myeloma drug lenalidomide promotes the cereblon-dependent 
            destruction of Ikaros proteins.
  JOURNAL   Science. 2014 Jan 17;343(6168):305-9. doi: 10.1126/science.1244917. 
            Epub 2013 Nov 29.
  PUBMED    24292623
REFERENCE   2  (bases 1 to 11339)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1244917"; journalName: "Science"; date: 
            "2014-01-17- 17"; volume: "343"; issue: "6168"; pages: "305-9"
COMMENT     Sequence Label: plenti-UbcP-FLAG-YWAA-pGK-HYG
FEATURES             Location/Qualifiers
     source          1..11339
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        78..1289
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1126..1145
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      1335..1344
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1344..1367
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    1389..1413
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2740..2764)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2908..3407
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2943..2966)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3340..3359
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3425..4450
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             3425..4450
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(4481..4514)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    4570..5158
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4623..4643)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5041..5052)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5161..5177)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5162..5178)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5367..5600
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5672..5793
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5709..5728)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5763..5782
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5833..5968
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5988..6007)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5989..6007)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(6030..6388)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6070..6091)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6282..6301
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6415..6519
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6521..6878
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6521..6712
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6544..6564)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6729..6864
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6913..7707
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6913..7707
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6967..6986)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7577..7596
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7895..7914)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7939..7986
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8315..8903
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8804..8823
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        9078..9381
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9383..9581
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9536..9556
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             9599..9779
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9826..9951
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10448..10681
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10866..10910
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11208..11325
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggacagcaga gatccagttt ggttagtacc gggcccgctc tagccctaga tttaacccaa
       61 cagaaggctc gacgcgtggc ctccgcgccg ggttttggcg cctcccgcgg gcgcccccct
      121 cctcacggcg agcgctgcca cgtcagacga agggcgcagc gagcgtcctg atccttccgc
      181 ccggacgctc aggacagcgg cccgctgctc ataagactcg gccttagaac cccagtatca
      241 gcagaaggac attttaggac gggacttggg tgactctagg gcactggttt tctttccaga
      301 gagcggaaca ggcgaggaaa agtagtccct tctcggcgat tctgcggagg gatctccgtg
      361 gggcggtgaa cgccgatgat tatataagga cgcgccgggt gtggcacagc tagttccgtc
      421 gcagccggga tttgggtcgc ggttcttgtt tgtggatcgc tgtgatcgtc acttggtgag
      481 tagcgggctg ctgggctggc cggggctttc gtggccgccg ggccgctcgg tgggacggaa
      541 gcgtgtggag agaccgccaa gggctgtagt ctgggtccgc gagcaaggtt gccctgaact
      601 gggggttggg gggagcgcag caaaatggcg gctgttcccg agtcttgaat ggaagacgct
      661 tgtgaggcgg gctgtgaggt cgttgaaaca aggtgggggg catggtgggc ggcaagaacc
      721 caaggtcttg aggccttcgc taatgcggga aagctcttat tcgggtgaga tgggctgggg
      781 caccatctgg ggaccctgac gtgaagtttg tcactgactg gagaactcgg tttgtcgtct
      841 gttgcggggg cggcagttat ggcggtgccg ttgggcagtg cacccgtacc tttgggagcg
      901 cgcgccctcg tcgtgtcgtg acgtcacccg ttctgttggc ttataatgca gggtggggcc
      961 acctgccggt aggtgtgcgg taggcttttc tccgtcgcag gacgcagggt tcgggcctag
     1021 ggtaggctct cctgaatcga caggcgccgg acctctggtg aggggaggga taagtgaggc
     1081 gtcagtttct ttggtcggtt ttatgtacct atcttcttaa gtagctgaag ctccggtttt
     1141 gaactatgcg ctcggggttg gcgagtgtgt tttgtgaagt tttttaggca ccttttgaaa
     1201 tgtaatcatt tgggtcaata tgtaattttc agtgttagac tagtaaattg tccgctaaat
     1261 tctggccgtt tttggctttt ttgttagacg ctagactgcc ggatcgaatt gtcgacgaat
     1321 tcggatccag atctgccacc atggactaca aggacgacga tgacaagggc ggagctcgag
     1381 gttatcaaac aagtttgtac aaaaaagttg gcatggccgg cgaaggagat cagcaggacg
     1441 ctgcgcacaa catgggcaac cacctgccgc tcctgcctga gagtgaggaa gaagatgaaa
     1501 tggaagttga agaccaggat agtaaagaag ccaaaaaacc aaacatcata aattttgaca
     1561 ccagtctgcc gacatcacat acatacctag gtgctgatat ggaagaattt catggcagga
     1621 ctttgcacga tgacgacagc tgtcaggtga ttccagttct tccacaagtg atgatgatcc
     1681 tgattcccgg acagacatta cctcttcagc tttttcaccc tcaagaagtc agtatggtgc
     1741 ggaatttaat tcagaaagat agaacctttg ctgttcttgc atacagcaat gtacaggaaa
     1801 gggaagcaca gtttggaaca acagcagaga tatatgccta tcgagaagaa caggattttg
     1861 gaattgagat agtgaaagtg aaagcaattg gaagacaaag gttcaaagtc cttgagctaa
     1921 gaacacagtc agatggaatc cagcaagcta aagtgcaaat tcttcccgaa tgtgtgttgc
     1981 cttcaaccat gtctgcagtt caattagaat ccctcaataa gtgccagata tttccttcaa
     2041 aacctgtctc aagagaagac caatgttcat ataaatggtg gcagaaatac cagaagagaa
     2101 agtttcattg tgcaaatcta acttcatggc ctcgctggct gtattcctta tatgatgctg
     2161 agaccttaat ggacagaatc aagaaacagc tacgtgaatg ggatgaaaat ctaaaagatg
     2221 attctcttcc ttcaaatcca atagattttt cttacagagt agctgcttgt cttcctattg
     2281 atgatgtatt gagaattcag ctccttaaaa ttggcagtgc tatccagcga cttcgctgtg
     2341 aattagacat tatgaataaa tgtacttccc tttgctgtaa acaatgtcaa gaaacagaaa
     2401 taacaaccaa aaatgaaata ttcagtttat ccttatgtgg gccgatggca gcttatgtga
     2461 atcctcatgg atatgtgcat gagacactta ctgtgtataa ggcttgcaac ttgaatctga
     2521 taggccggcc ttctacagaa cacagctggt ttcctggggc tgccgcgact gttgcccagt
     2581 gtaagatctg tgcaagccat attggatgga agtttacggc caccaaaaaa gacatgtcac
     2641 ctcaaaaatt ttggggctta acgcgatctg ctctgttgcc cacgatccca gacactgaag
     2701 atgaaataag tccagacaaa gtaatacttt gcttgtaaga cccagctttc ttgtacaaag
     2761 tggttcgata acgaattccg cccccccccc ctaacgttac tggccgaagc cgcttggaat
     2821 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg
     2881 tgagggccgc gtttaacgaa ttctaccggg taggggaggc gcttttccca aggcagtctg
     2941 gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc
     3001 gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg
     3061 cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt
     3121 gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg
     3181 ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg
     3241 ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca
     3301 ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc
     3361 gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcatcccgc
     3421 caccatgaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt
     3481 cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt
     3541 cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa
     3601 agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga
     3661 cattggggaa ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac
     3721 gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat
     3781 ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca
     3841 aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt
     3901 gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga
     3961 tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt
     4021 cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga
     4081 ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt
     4141 ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc
     4201 gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt
     4261 tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc
     4321 cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga
     4381 tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc
     4441 aaaggaatag tgtacaagta ggaattcgtc gagggaccta ataacttcgt atagcataca
     4501 ttatacgaag ttatacatgt ttaagggttc cggttccact aggtacaatt cgatatcaag
     4561 cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     4621 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     4681 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     4741 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     4801 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     4861 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     4921 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     4981 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     5041 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     5101 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat
     5161 cgataccgtc gacctcgatc gagacctaga aaaacatgga gcaatcacaa gtagcaatac
     5221 agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt
     5281 tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     5341 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     5401 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     5461 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     5521 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     5581 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     5641 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     5701 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     5761 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     5821 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     5881 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     5941 aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt
     6001 cgtattagca tgtgagcaaa aggccagcat aaatcaaaag aatagaccga gatagggttg
     6061 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     6121 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     6181 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     6241 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     6301 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     6361 gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga
     6421 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     6481 ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc
     6541 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     6601 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     6661 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     6721 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     6781 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     6841 agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga
     6901 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     6961 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     7021 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     7081 ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     7141 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     7201 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     7261 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     7321 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     7381 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     7441 agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     7501 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     7561 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     7621 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     7681 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag
     7741 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg
     7801 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     7861 tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg
     7921 aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt
     7981 ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac
     8041 cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg
     8101 gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt
     8161 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     8221 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     8281 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     8341 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     8401 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     8461 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     8521 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     8581 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     8641 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     8701 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     8761 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     8821 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     8881 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     8941 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     9001 gtattaccgc catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc
     9061 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     9121 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     9181 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     9241 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     9301 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     9361 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     9421 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     9481 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     9541 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg cctgtactgg
     9601 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     9661 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     9721 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     9781 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
     9841 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
     9901 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
     9961 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
    10021 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
    10081 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
    10141 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
    10201 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
    10261 aacaaaagta agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag
    10321 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
    10381 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
    10441 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
    10501 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
    10561 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
    10621 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
    10681 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
    10741 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
    10801 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
    10861 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
    10921 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
    10981 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
    11041 caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa
    11101 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatcgg
    11161 cactgcgtgc gccaattctg cagacaaatg gcagtattca tccacaattt taaaagaaaa
    11221 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
    11281 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacag
//
