LOCUS       sequence-210552-       11345 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of myc-CRBN.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11345)
  AUTHORS   Lu G, Middleton RE, Sun H, Naniong M, Ott CJ, Mitsiades CS, Wong KK,
            Bradner JE, Kaelin WG Jr
  TITLE     The myeloma drug lenalidomide promotes the cereblon-dependent 
            destruction of Ikaros proteins.
  JOURNAL   Science. 2014 Jan 17;343(6168):305-9. doi: 10.1126/science.1244917. 
            Epub 2013 Nov 29.
  PUBMED    24292623
REFERENCE   2  (bases 1 to 11345)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1244917"; journalName: "Science"; date: 
            "2014-01-17- 17"; volume: "343"; issue: "6168"; pages: "305-9"
COMMENT     Sequence Label: plenti-UbcP-Myc-CRBN-pGK-HYG
FEATURES             Location/Qualifiers
     source          1..11345
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    29..146
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        238..1449
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1286..1305
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      1495..1504
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1504..1533
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    1555..1579
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2906..2930)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        3074..3573
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3109..3132)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3506..3525
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3591..4616
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             3591..4616
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(4647..4680)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    4736..5324
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4789..4809)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5207..5218)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5327..5343)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5328..5344)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5533..5766
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5838..5959
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5875..5894)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5929..5948
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5999..6134
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6154..6173)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6155..6173)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(6196..6554)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6236..6257)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6448..6467
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6581..6685
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6687..7044
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6687..6878
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6710..6730)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6895..7030
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7079..7873
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7079..7873
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7133..7152)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7743..7762
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8061..8080)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8105..8152
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8481..9069
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8970..8989
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        9244..9547
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9549..9747
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9702..9722
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             9765..9945
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9992..10117
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10614..10847
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11032..11076
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gcagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca
       61 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa
      121 aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt
      181 ggttagtacc gggcccgctc tagccctaga tttaacccaa cagaaggctc gacgcgtggc
      241 ctccgcgccg ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca
      301 cgtcagacga agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg
      361 cccgctgctc ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac
      421 gggacttggg tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa
      481 agtagtccct tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat
      541 tatataagga cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc
      601 ggttcttgtt tgtggatcgc tgtgatcgtc acttggtgag tagcgggctg ctgggctggc
      661 cggggctttc gtggccgccg ggccgctcgg tgggacggaa gcgtgtggag agaccgccaa
      721 gggctgtagt ctgggtccgc gagcaaggtt gccctgaact gggggttggg gggagcgcag
      781 caaaatggcg gctgttcccg agtcttgaat ggaagacgct tgtgaggcgg gctgtgaggt
      841 cgttgaaaca aggtgggggg catggtgggc ggcaagaacc caaggtcttg aggccttcgc
      901 taatgcggga aagctcttat tcgggtgaga tgggctgggg caccatctgg ggaccctgac
      961 gtgaagtttg tcactgactg gagaactcgg tttgtcgtct gttgcggggg cggcagttat
     1021 ggcggtgccg ttgggcagtg cacccgtacc tttgggagcg cgcgccctcg tcgtgtcgtg
     1081 acgtcacccg ttctgttggc ttataatgca gggtggggcc acctgccggt aggtgtgcgg
     1141 taggcttttc tccgtcgcag gacgcagggt tcgggcctag ggtaggctct cctgaatcga
     1201 caggcgccgg acctctggtg aggggaggga taagtgaggc gtcagtttct ttggtcggtt
     1261 ttatgtacct atcttcttaa gtagctgaag ctccggtttt gaactatgcg ctcggggttg
     1321 gcgagtgtgt tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt tgggtcaata
     1381 tgtaattttc agtgttagac tagtaaattg tccgctaaat tctggccgtt tttggctttt
     1441 ttgttagacg ctagactgcc ggatcgaatt gtcgacgaat tcggatccag atctgccacc
     1501 atggagcaga agctgatctc tgaggaggat ctgggaggcg ctcgaggtta tcaaacaagt
     1561 ttgtacaaaa aagttggcat ggccggcgaa ggagatcagc aggacgctgc gcacaacatg
     1621 ggcaaccacc tgccgctcct gcctgagagt gaggaagaag atgaaatgga agttgaagac
     1681 caggatagta aagaagccaa aaaaccaaac atcataaatt ttgacaccag tctgccgaca
     1741 tcacatacat acctaggtgc tgatatggaa gaatttcatg gcaggacttt gcacgatgac
     1801 gacagctgtc aggtgattcc agttcttcca caagtgatga tgatcctgat tcccggacag
     1861 acattacctc ttcagctttt tcaccctcaa gaagtcagta tggtgcggaa tttaattcag
     1921 aaagatagaa cctttgctgt tcttgcatac agcaatgtac aggaaaggga agcacagttt
     1981 ggaacaacag cagagatata tgcctatcga gaagaacagg attttggaat tgagatagtg
     2041 aaagtgaaag caattggaag acaaaggttc aaagtccttg agctaagaac acagtcagat
     2101 ggaatccagc aagctaaagt gcaaattctt cccgaatgtg tgttgccttc aaccatgtct
     2161 gcagttcaat tagaatccct caataagtgc cagatatttc cttcaaaacc tgtctcaaga
     2221 gaagaccaat gttcatataa atggtggcag aaataccaga agagaaagtt tcattgtgca
     2281 aatctaactt catggcctcg ctggctgtat tccttatatg atgctgagac cttaatggac
     2341 agaatcaaga aacagctacg tgaatgggat gaaaatctaa aagatgattc tcttccttca
     2401 aatccaatag atttttctta cagagtagct gcttgtcttc ctattgatga tgtattgaga
     2461 attcagctcc ttaaaattgg cagtgctatc cagcgacttc gctgtgaatt agacattatg
     2521 aataaatgta cttccctttg ctgtaaacaa tgtcaagaaa cagaaataac aaccaaaaat
     2581 gaaatattca gtttatcctt atgtgggccg atggcagctt atgtgaatcc tcatggatat
     2641 gtgcatgaga cacttactgt gtataaggct tgcaacttga atctgatagg ccggccttct
     2701 acagaacaca gctggtttcc tgggtatgcc tggactgttg cccagtgtaa gatctgtgca
     2761 agccatattg gatggaagtt tacggccacc aaaaaagaca tgtcacctca aaaattttgg
     2821 ggcttaacgc gatctgctct gttgcccacg atcccagaca ctgaagatga aataagtcca
     2881 gacaaagtaa tactttgctt gtaagaccca gctttcttgt acaaagtggt tcgataacga
     2941 attccgcccc ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg
     3001 tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag ggccgcgttt
     3061 aacgaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta
     3121 gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca
     3181 tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact
     3241 cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa
     3301 atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag
     3361 cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag
     3421 aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc
     3481 gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg
     3541 ctgttctcct cttcctcatc tccgggcctt tcgacctgca tcccgccacc atgaaaaagc
     3601 ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg
     3661 acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc
     3721 gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt
     3781 atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattca
     3841 gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc
     3901 ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat gcgatcgctg
     3961 cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat
     4021 acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa
     4081 ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt
     4141 gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg
     4201 tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg
     4261 attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc
     4321 agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg cggctccggg
     4381 cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg
     4441 atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg
     4501 tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag
     4561 tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag gaatagtgta
     4621 caagtaggaa ttcgtcgagg gacctaataa cttcgtatag catacattat acgaagttat
     4681 acatgtttaa gggttccggt tccactaggt acaattcgat atcaagctta tcgataatca
     4741 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     4801 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     4861 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     4921 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     4981 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     5041 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     5101 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     5161 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     5221 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     5281 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc
     5341 tcgatcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg
     5401 ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc
     5461 aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     5521 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     5581 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     5641 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     5701 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     5761 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     5821 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     5881 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     5941 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     6001 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     6061 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     6121 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttagcatgtg
     6181 agcaaaaggc cagcataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
     6241 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
     6301 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
     6361 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
     6421 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
     6481 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
     6541 gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     6601 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     6661 tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt
     6721 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     6781 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     6841 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     6901 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     6961 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     7021 tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat
     7081 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     7141 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     7201 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     7261 agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     7321 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     7381 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     7441 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     7501 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     7561 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg
     7621 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     7681 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     7741 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     7801 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     7861 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
     7921 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     7981 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     8041 caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct
     8101 atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc
     8161 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa
     8221 tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg
     8281 ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt
     8341 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     8401 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     8461 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     8521 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     8581 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     8641 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     8701 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     8761 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     8821 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     8881 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     8941 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     9001 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     9061 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     9121 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg
     9181 cattagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
     9241 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     9301 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     9361 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     9421 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     9481 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     9541 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
     9601 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     9661 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
     9721 tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct ctctggttag
     9781 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     9841 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     9901 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
     9961 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
    10021 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
    10081 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
    10141 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
    10201 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
    10261 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
    10321 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
    10381 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
    10441 caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga tatgagggac
    10501 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
    10561 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
    10621 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
    10681 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
    10741 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
    10801 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
    10861 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
    10921 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
    10981 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
    11041 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
    11101 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
    11161 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
    11221 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
    11281 ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact gcgtgcgcca
    11341 attct
//
