LOCUS       sequence-210569-        7938 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Truncated Hsh155 (aa 64-972).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7938)
  AUTHORS   Carrocci TJ, Paulson JC, Hoskins AA
  TITLE     Functional Analysis of Hsh155/SF3b1 Interactions with the U2 
            snRNA/Branch Site Duplex.
  JOURNAL   RNA. 2018 May 11. pii: rna.065664.118. doi: 10.1261/rna.065664.118.
  PUBMED    29752352
REFERENCE   2  (bases 1 to 7938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2018 
            May 11. pii: rna.065664.118. doi: 10.1261/rna.065664.118."
COMMENT     Sequence Label: Hsh155 64-972
FEATURES             Location/Qualifiers
     source          1..7938
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        187..467
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             468..1142
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(1241..1696)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1378..1399)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1590..1609
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1826..1848
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1840..1857
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1841..1857
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1867..1886
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1867..1885
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5164..5184)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5164..5182)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5203..5219)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5203..5219)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5216..5238)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5227..5243
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5251..5257,5258..5275,5276..5281))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(5434..5451)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5605..6193)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5685..5704)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6364..7155,7156..7224))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6987..7006
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7225..7329)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    7366..7869
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     7911..7929
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaacg acattactat atatataata taggaagcat ttaatagaca gcatcgtaat
      241 atatgtgtac tttgcagtta tgacgccaga tggcagtagt ggaagatatt ctttattgaa
      301 aaatagcttg tcaccttacg tacaatcttg atccggagct tttctttttt tgccgattaa
      361 gaattaattc ggtcgaaaaa agaaaaggag agggccaaga gggagggcat tggtgactat
      421 tgagcacgtg agtatacgtg attaagcaca caaaggcagc ttggagtatg tctgttatta
      481 atttcacagg tagttctggt ccattggtga aagtttgcgg cttgcagagc acagaggccg
      541 cagaatgtgc tctagattcc gatgctgact tgctgggtat tatatgtgtg cccaatagaa
      601 agagaacaat tgacccggtt attgcaagga aaatttcaag tcttgtaaaa gcatataaaa
      661 atagttcagg cactccgaaa tacttggttg gcgtgtttcg taatcaacct aaggaggatg
      721 ttttggctct ggtcaatgat tacggcattg atatcgtcca actgcatgga gatgagtcgt
      781 ggcaagaata ccaagagttc ctcggtttgc cagttattaa aagactcgta tttccaaaag
      841 actgcaacat actactcagt gcagcttcac agaaacctca ttcgtttatt cccttgtttg
      901 attcagaagc aggtgggaca ggtgaacttt tggattggaa ctcgatttct gactgggttg
      961 gaaggcaaga gagccccgaa agcttacatt ttatgttagc tggtggactg acgccagaaa
     1021 atgttggtga tgcgcttaga ttaaatggcg ttattggtgt tgatgtaagc ggaggtgtgg
     1081 agacaaatgg tgtaaaagac tctaacaaaa tagcaaattt cgtcaaaaat gctaagaaat
     1141 aggttattac tgagtagtat ttatttaagt attgtttgtg cacttgccta tgcggtgtga
     1201 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt
     1261 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     1321 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     1381 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     1441 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     1501 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     1561 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     1621 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     1681 ccgctacagg gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     1741 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     1801 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag
     1861 cgcgcgtaat acgactcact atagggcgaa ttgggtaccg ggccccccct cgagcatgta
     1921 ctttccgcct aactaccatc acctgtcttt atttattccg ctgacagata tgcacaacca
     1981 attatcgtct cttgaaagct ttttagacgt gattgctgtt atttggcacg ttgttagtat
     2041 ttaagcttat tcgggtttgc attgatattt gcccgagcag aaatctcctt ctcgatgagt
     2101 tagaactgaa acatctcttc aattacttat acataataaa catagaaagc caggacctgt
     2161 catcatgtac cacaaaagaa ggtttgatat gaagtttgag ccggattccg atacacaaac
     2221 tgttacgtct tcagaaaata cccaagacgc tgtcgtgcca aggaaaagaa aatctcgttg
     2281 ggatgtaaaa gggtacgagc cacctgatga atcttcaaca gcggtcaaag aaaactctga
     2341 tagtgctttg gttaatgtag aaggaatcca tgatttgatg ttttttaaac cttctgacca
     2401 taaatatttc gccgacgtta tatccaaaaa acccatagat gagttaaata aggatgaaaa
     2461 gaaagaaaga accctgtcga tgttacttct gaaaataaaa aatggcaata cagcgagtag
     2521 gaggacttct atgcggattt taaccgataa agctgttaca tttggtccag aaatgatatt
     2581 taaccgctta ttgcccatat tactagacag aagtttggaa gaccaggaaa gacatctaat
     2641 gatcaagacc atagatcgcg tcctttacca gctaggagat ttaacaaaac cttacgttca
     2701 caaaatactt gtagtagctg ccccattgct aatagatgaa gatccaatgg ttcgttcaac
     2761 aggccaggag ataatcacca atctgtctac agtcgctggc ctaaaaacca ttctaacagt
     2821 aatgagacct gatattgaga atgaggatga gtatgttaga aatgtaactt caagagcggc
     2881 ggctgtggtg gcaaaagccc ttggtgttaa tcaattgctt cctttcatca atgcagcatg
     2941 ccactcacgg aagtcttgga aagcaaggca tactggcata aaaattgtgc agcaaatagg
     3001 tatacttttg ggaataggtg tcttgaatca tcttactggg ctgatgagtt gtatcaaaga
     3061 ctgcctaatg gatgatcacg ttccggtgcg aattgttaca gcacatactt tatcaacact
     3121 agcagaaaat tcttatccat atggtatcga agttttcaac gttgtactgg agccattatg
     3181 gaagggtata agaagtcatc gtggtaaagt gctatcttct tttttaaaag ctgttggatc
     3241 tatgatccca ttgatggacc cagagtatgc cggttattat accacagaag caatgagaat
     3301 aattagacgt gaattcgact ctcccgatga tgaaatgaaa aagaccattc ttttagtatt
     3361 acaaaaatgt agcgctgtag aatccataac gccaaaattt ctgagagaag aaatcgcgcc
     3421 agaattcttt caaaaatttt gggtcagacg agtggcattg gatagaccat taaacaaagt
     3481 tgttacatat actactgtta cattagcaaa gaaattaggt tgttcttata ccattgataa
     3541 attattaaca ccattgagag atgaagctga gccgttcaga acaatggctg tccatgctgt
     3601 tactagaaca gtaaacttat tagggacagc agatttagat gagagattag aaacgaggct
     3661 tattgatgcg ctcttgatag catttcaaga acaaacaaat agtgattcta taatctttaa
     3721 agggtttggg gcagtgacgg tgtcacttga catccgtatg aaaccgtttt tagcacccat
     3781 tgtgagtacg attttaaatc atttaaagca taaaactccc ttggttcgtc aacatgcagc
     3841 agatctatgt gccatactaa taccagtcat caagaactgt cacgaattcg aaatgttgaa
     3901 taaattgaac attatattgt atgaatcctt aggcgaagta tatccagagg tattaggttc
     3961 tataatcaac gctatgtact gtattactag cgtaatggat ttggataaac tacaacctcc
     4021 tattaatcaa attttaccaa ccttaactcc aattttaaga aacaagcata gaaaagtcga
     4081 agtaaacact atcaaatttg ttggacttat tggtaaatta gcacctacct atgcacctcc
     4141 taaagaatgg atgagaatat gttttgaact attagaactt ttgaaaagta caaataaaga
     4201 aataagaaga tcagcaaatg ccacgtttgg attcattgct gaagcaattg gacctcacga
     4261 tgttcttgtt gcgttattga acaatttaaa ggttcaagaa cgtcagttac gtgtatgcac
     4321 ggctgttgct atcggtatcg ttgctaaagt gtgtggtcct tacaacgtgc tgccagtaat
     4381 catgaatgaa tacacaacac cagagactaa tgttcaaaac ggtgttctca aggccatgtc
     4441 attcatgttt gagtacattg gtaacatgtc taaggattac atatacttca taacaccatt
     4501 attagaagat gcactcaccg atagggattt agttcatcgt cagacagcgt cgaacgtaat
     4561 aacccactta gctttaaact gttcaggtac aggtcacgaa gatgccttca ttcatttaat
     4621 gaatcttcta atacccaata tttttgaaac ttcaccacat gctattatgc gtattttaga
     4681 aggtttggag gcactgagtc aagctcttgg ccctggacta ttcatgaact acatatgggc
     4741 aggattgttc catccagcga agaatgtaag gaaagcattt tggagggtgt ataataacat
     4801 gtacgtaatg taccaggacg ccatggtacc tttttacccc gttacaccag acaacaatga
     4861 agaatatata gaagaactgg atttagttct gtgaatatat atagagcata taaataacca
     4921 caacttgtaa gaatatttta cttgacacac ctatatcatc acgtcacgat gccttcatag
     4981 attatgatga aatataaaca gaacctgcta ttggaaaaaa aagggcagga aaaaattttg
     5041 tgaaagtgag taaaagaaag atgaagagcg agacatggat aataagaaat agatgtcact
     5101 aatggacaat tggaagactg atatggaaag ttacgatgaa ggaggagctc ccagcttttg
     5161 ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt
     5221 gtgaaattgt tatccgctca caattccaca caacatagga gccggaagca taaagtgtaa
     5281 agcctggggt gcctaatgag tgaggtaact cacattaatt gcgttgcgct cactgcccgc
     5341 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     5401 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     5461 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     5521 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     5581 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     5641 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     5701 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     5761 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     5821 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     5881 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     5941 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     6001 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     6061 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     6121 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     6181 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     6241 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     6301 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     6361 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     6421 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     6481 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     6541 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     6601 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     6661 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     6721 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     6781 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     6841 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     6901 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     6961 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     7021 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     7081 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     7141 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     7201 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     7261 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     7321 ggttccgcgc acatttcccc gaaaagtgcc acctgggtcc ttttcatcac gtgctataaa
     7381 aataattata atttaaattt tttaatataa atatataaat taaaaataga aagtaaaaaa
     7441 agaaattaaa gaaaaaatag tttttgtttt ccgaagatgt aaaagactct agggggatcg
     7501 ccaacaaata ctacctttta tcttgctctt cctgctctca ggtattaatg ccgaattgtt
     7561 tcatcttgtc tgtgtagaag accacacacg aaaatcctgt gattttacat tttacttatc
     7621 gttaatcgaa tgtatatcta tttaatctgc ttttcttgtc taataaatat atatgtaaag
     7681 tacgcttttt gttgaaattt tttaaacctt tgtttatttt tttttcttca ttccgtaact
     7741 cttctacctt ctttatttac tttctaaaat ccaaatacaa aacataaaaa taaataaaca
     7801 cagagtaaat tcccaaatta ttccatcatt aaaagatacg aggcgcgtgt aagttacagg
     7861 caagcgatcc gtcctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     7921 tcacgaggcc ctttcgtc
//
