LOCUS       sequence-210575-        6183 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Membrane Receptor, Excitation: 505 / 569, Emission: 
            516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6183)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 6183)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos2-ALK5-N-13
FEATURES             Location/Qualifiers
     source          1..6183
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        119..422
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        423..626
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     577..597
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      673..682
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2227..2907
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     polyA_signal    3029..3150
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3066..3085)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3120..3139
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3157..3612)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3294..3315)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3506..3525
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3639..3743
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3745..4102
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3745..3936
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3768..3788)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3953..4088
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4137..4931
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4137..4931
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4191..4210)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4801..4820
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5119..5138)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5163..5210
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5539..6127
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6028..6047
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcatta
       61 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      121 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      181 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      241 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      301 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      361 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      421 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
      481 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      541 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
      601 ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc
      661 ggactcagat ctgccaccat ggaggcggcg gtcgctgctc cgcgtccccg gctgctcctc
      721 ctcgtgctgg cggcggcggc ggcggcggcg gcggcgctgc tcccgggggc gacggcgtta
      781 cagtgtttct gccacctctg tacaaaagac aattttactt gtgtgacaga tgggctctgc
      841 tttgtctctg tcacagagac cacagacaaa gttatacaca acagcatgtg tatagctgaa
      901 attgacttaa ttcctcgaga taggccgttt gtatgtgcac cctcttcaaa aactgggtct
      961 gtgactacaa catattgctg caatcaggac cattgcaata aaatagaact tccaactact
     1021 gtaaagtcat cacctggcct tggtcctgtg gaactggcag ctgtcattgc tggaccagtg
     1081 tgcttcgtct gcatctcact catgttgatg gtctatatct gccacaaccg cactgtcatt
     1141 caccatcgag tgccaaatga agaggaccct tcattagatc gcccttttat ttcagagggt
     1201 actacgttga aagacttaat ttatgatatg acaacgtcag gttctggctc aggtttacca
     1261 ttgcttgttc agagaacaat tgcgagaact attgtgttac aagaaagcat tggcaaaggt
     1321 cgatttggag aagtttggag aggaaagtgg cggggagaag aagttgctgt taagatattc
     1381 tcctctagag aagaacgttc gtggttccgt gaggcagaga tttatcaaac tgtaatgtta
     1441 cgtcatgaaa acatcctggg atttatagca gcagacaata aagacaatgg tacttggact
     1501 cagctctggt tggtgtcaga ttatcatgag catggatccc tttttgatta cttaaacaga
     1561 tacacagtta ctgtggaagg aatgataaaa cttgctctgt ccacggcgag cggtcttgcc
     1621 catcttcaca tggagattgt tggtacccaa ggaaagccag ccattgctca tagagatttg
     1681 aaatcaaaga atatcttggt aaagaagaat ggaacttgct gtattgcaga cttaggactg
     1741 gcagtaagac atgattcagc cacagatacc attgatattg ctccaaacca cagagtggga
     1801 acaaaaaggt acatggcccc tgaagttctc gatgattcca taaatatgaa acattttgaa
     1861 tccttcaaac gtgctgacat ctatgcaatg ggcttagtat tctgggaaat tgctcgacga
     1921 tgttccattg gtggaattca tgaagattac caactgcctt attatgatct tgtaccttct
     1981 gacccatcag ttgaagaaat gagaaaagtt gtttgtgaac agaagttaag gccaaatatc
     2041 ccaaacagat ggcagagctg tgaagccttg agagtaatgg ctaaaattat gagagaatgt
     2101 tggtatgcca atggagcagc taggcttaca gcattgcgga ttaagaaaac attatcgcaa
     2161 ctcagtcaac aggaaggcat caaaatgggt tccgggggtg cgggcggcgg aggaccggtc
     2221 gccaccatga gtgcgattaa gccagacatg aagatcaaac tccgtatgga aggcaacgta
     2281 aacgggcacc actttgtgat cgacggagat ggtacaggca agccttttga gggaaaacag
     2341 agtatggatc ttgaagtcaa agagggcgga cctctgcctt ttgcctttga tatcctgacc
     2401 actgcattcc attacggcaa cagggtattc gccaaatatc cagacaacat acaagactat
     2461 tttaagcagt cgtttcctaa ggggtattcg tgggaacgaa gcttgacttt cgaagacggg
     2521 ggcatttgca ttgccagaaa cgacataaca atggaagggg acactttcta taataaagtt
     2581 cgattttatg gtaccaactt tcccgccaat ggtccagtta tgcagaagaa gacgctgaaa
     2641 tgggagccct ccactgagaa aatgtatgtg cgtgatggag tgctgacggg tgatattcat
     2701 atggctttgt tgcttgaagg aaatgcccat taccgatgtg acttcagaac tacttacaaa
     2761 gctaaggaga agggtgtcaa gttaccaggc taccactttg tggaccactg cattgagatt
     2821 ttaagccatg acaaagatta caacaaggtt aagctgtatg agcatgctgt tgctcattct
     2881 ggattgcctg acaatgccag acgataagcg gccgcgactc tagatcataa tcagccatac
     2941 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     3001 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     3061 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     3121 tggtttgtcc aaactcatca atgtatctta aggcgtaaat tgtaagcgtt aatattttgt
     3181 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
     3241 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
     3301 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     3361 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
     3421 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     3481 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     3541 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     3601 tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     3661 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     3721 aataatattg aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca
     3781 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     3841 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     3901 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     3961 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     4021 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     4081 tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga
     4141 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     4201 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     4261 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag
     4321 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     4381 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     4441 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     4501 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     4561 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     4621 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg
     4681 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     4741 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     4801 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     4861 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     4921 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
     4981 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     5041 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     5101 ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat
     5161 gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg
     5221 ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata
     5281 cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct
     5341 cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta
     5401 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     5461 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     5521 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     5581 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     5641 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     5701 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     5761 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     5821 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     5881 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     5941 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     6001 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     6061 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     6121 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     6181 tca
//
