LOCUS       sequence-210579-        7842 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Truncated Hsh155 (aa 95-972).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7842)
  AUTHORS   Carrocci TJ, Paulson JC, Hoskins AA
  TITLE     Functional Analysis of Hsh155/SF3b1 Interactions with the U2 
            snRNA/Branch Site Duplex.
  JOURNAL   RNA. 2018 May 11. pii: rna.065664.118. doi: 10.1261/rna.065664.118.
  PUBMED    29752352
REFERENCE   2  (bases 1 to 7842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2018 
            May 11. pii: rna.065664.118. doi: 10.1261/rna.065664.118."
COMMENT     Sequence Label: Hsh155 96-972
FEATURES             Location/Qualifiers
     source          1..7842
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        187..467
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             468..1142
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(1241..1696)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1378..1399)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1590..1609
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1826..1848
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1840..1857
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1841..1857
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1867..1886
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1867..1885
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5068..5088)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5068..5086)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5107..5123)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5107..5123)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5120..5142)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5131..5147
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5155..5161,5162..5179,5180..5185))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(5338..5355)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5509..6097)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5589..5608)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6268..7059,7060..7128))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6891..6910
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7129..7233)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    7270..7773
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     7815..7833
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaacg acattactat atatataata taggaagcat ttaatagaca gcatcgtaat
      241 atatgtgtac tttgcagtta tgacgccaga tggcagtagt ggaagatatt ctttattgaa
      301 aaatagcttg tcaccttacg tacaatcttg atccggagct tttctttttt tgccgattaa
      361 gaattaattc ggtcgaaaaa agaaaaggag agggccaaga gggagggcat tggtgactat
      421 tgagcacgtg agtatacgtg attaagcaca caaaggcagc ttggagtatg tctgttatta
      481 atttcacagg tagttctggt ccattggtga aagtttgcgg cttgcagagc acagaggccg
      541 cagaatgtgc tctagattcc gatgctgact tgctgggtat tatatgtgtg cccaatagaa
      601 agagaacaat tgacccggtt attgcaagga aaatttcaag tcttgtaaaa gcatataaaa
      661 atagttcagg cactccgaaa tacttggttg gcgtgtttcg taatcaacct aaggaggatg
      721 ttttggctct ggtcaatgat tacggcattg atatcgtcca actgcatgga gatgagtcgt
      781 ggcaagaata ccaagagttc ctcggtttgc cagttattaa aagactcgta tttccaaaag
      841 actgcaacat actactcagt gcagcttcac agaaacctca ttcgtttatt cccttgtttg
      901 attcagaagc aggtgggaca ggtgaacttt tggattggaa ctcgatttct gactgggttg
      961 gaaggcaaga gagccccgaa agcttacatt ttatgttagc tggtggactg acgccagaaa
     1021 atgttggtga tgcgcttaga ttaaatggcg ttattggtgt tgatgtaagc ggaggtgtgg
     1081 agacaaatgg tgtaaaagac tctaacaaaa tagcaaattt cgtcaaaaat gctaagaaat
     1141 aggttattac tgagtagtat ttatttaagt attgtttgtg cacttgccta tgcggtgtga
     1201 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt
     1261 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     1321 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     1381 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     1441 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     1501 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     1561 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     1621 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     1681 ccgctacagg gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     1741 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     1801 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag
     1861 cgcgcgtaat acgactcact atagggcgaa ttgggtaccg ggccccccct cgagcatgta
     1921 ctttccgcct aactaccatc acctgtcttt atttattccg ctgacagata tgcacaacca
     1981 attatcgtct cttgaaagct ttttagacgt gattgctgtt atttggcacg ttgttagtat
     2041 ttaagcttat tcgggtttgc attgatattt gcccgagcag aaatctcctt ctcgatgagt
     2101 tagaactgaa acatctcttc aattacttat acataataaa catagaaagc caggacctgt
     2161 catcatgaaa agaaaatctc gttgggatgt aaaagggtac gagccacctg atgaatcttc
     2221 aacagcggtc aaagaaaact ctgatagtgc tttggttaat gtagaaggaa tccatgattt
     2281 gatgtttttt aaaccttctg accataaata tttcgccgac gttatatcca aaaaacccat
     2341 agatgagtta aataaggatg aaaagaaaga aagaaccctg tcgatgttac ttctgaaaat
     2401 aaaaaatggc aatacagcga gtaggaggac ttctatgcgg attttaaccg ataaagctgt
     2461 tacatttggt ccagaaatga tatttaaccg cttattgccc atattactag acagaagttt
     2521 ggaagaccag gaaagacatc taatgatcaa gaccatagat cgcgtccttt accagctagg
     2581 agatttaaca aaaccttacg ttcacaaaat acttgtagta gctgccccat tgctaataga
     2641 tgaagatcca atggttcgtt caacaggcca ggagataatc accaatctgt ctacagtcgc
     2701 tggcctaaaa accattctaa cagtaatgag acctgatatt gagaatgagg atgagtatgt
     2761 tagaaatgta acttcaagag cggcggctgt ggtggcaaaa gcccttggtg ttaatcaatt
     2821 gcttcctttc atcaatgcag catgccactc acggaagtct tggaaagcaa ggcatactgg
     2881 cataaaaatt gtgcagcaaa taggtatact tttgggaata ggtgtcttga atcatcttac
     2941 tgggctgatg agttgtatca aagactgcct aatggatgat cacgttccgg tgcgaattgt
     3001 tacagcacat actttatcaa cactagcaga aaattcttat ccatatggta tcgaagtttt
     3061 caacgttgta ctggagccat tatggaaggg tataagaagt catcgtggta aagtgctatc
     3121 ttctttttta aaagctgttg gatctatgat cccattgatg gacccagagt atgccggtta
     3181 ttataccaca gaagcaatga gaataattag acgtgaattc gactctcccg atgatgaaat
     3241 gaaaaagacc attcttttag tattacaaaa atgtagcgct gtagaatcca taacgccaaa
     3301 atttctgaga gaagaaatcg cgccagaatt ctttcaaaaa ttttgggtca gacgagtggc
     3361 attggataga ccattaaaca aagttgttac atatactact gttacattag caaagaaatt
     3421 aggttgttct tataccattg ataaattatt aacaccattg agagatgaag ctgagccgtt
     3481 cagaacaatg gctgtccatg ctgttactag aacagtaaac ttattaggga cagcagattt
     3541 agatgagaga ttagaaacga ggcttattga tgcgctcttg atagcatttc aagaacaaac
     3601 aaatagtgat tctataatct ttaaagggtt tggggcagtg acggtgtcac ttgacatccg
     3661 tatgaaaccg tttttagcac ccattgtgag tacgatttta aatcatttaa agcataaaac
     3721 tcccttggtt cgtcaacatg cagcagatct atgtgccata ctaataccag tcatcaagaa
     3781 ctgtcacgaa ttcgaaatgt tgaataaatt gaacattata ttgtatgaat ccttaggcga
     3841 agtatatcca gaggtattag gttctataat caacgctatg tactgtatta ctagcgtaat
     3901 ggatttggat aaactacaac ctcctattaa tcaaatttta ccaaccttaa ctccaatttt
     3961 aagaaacaag catagaaaag tcgaagtaaa cactatcaaa tttgttggac ttattggtaa
     4021 attagcacct acctatgcac ctcctaaaga atggatgaga atatgttttg aactattaga
     4081 acttttgaaa agtacaaata aagaaataag aagatcagca aatgccacgt ttggattcat
     4141 tgctgaagca attggacctc acgatgttct tgttgcgtta ttgaacaatt taaaggttca
     4201 agaacgtcag ttacgtgtat gcacggctgt tgctatcggt atcgttgcta aagtgtgtgg
     4261 tccttacaac gtgctgccag taatcatgaa tgaatacaca acaccagaga ctaatgttca
     4321 aaacggtgtt ctcaaggcca tgtcattcat gtttgagtac attggtaaca tgtctaagga
     4381 ttacatatac ttcataacac cattattaga agatgcactc accgataggg atttagttca
     4441 tcgtcagaca gcgtcgaacg taataaccca cttagcttta aactgttcag gtacaggtca
     4501 cgaagatgcc ttcattcatt taatgaatct tctaataccc aatatttttg aaacttcacc
     4561 acatgctatt atgcgtattt tagaaggttt ggaggcactg agtcaagctc ttggccctgg
     4621 actattcatg aactacatat gggcaggatt gttccatcca gcgaagaatg taaggaaagc
     4681 attttggagg gtgtataata acatgtacgt aatgtaccag gacgccatgg taccttttta
     4741 ccccgttaca ccagacaaca atgaagaata tatagaagaa ctggatttag ttctgtgaat
     4801 atatatagag catataaata accacaactt gtaagaatat tttacttgac acacctatat
     4861 catcacgtca cgatgccttc atagattatg atgaaatata aacagaacct gctattggaa
     4921 aaaaaagggc aggaaaaaat tttgtgaaag tgagtaaaag aaagatgaag agcgagacat
     4981 ggataataag aaatagatgt cactaatgga caattggaag actgatatgg aaagttacga
     5041 tgaaggagga gctcccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta
     5101 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     5161 aggagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgaggt aactcacatt
     5221 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     5281 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     5341 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     5401 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     5461 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     5521 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     5581 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     5641 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     5701 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     5761 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     5821 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     5881 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     5941 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     6001 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     6061 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     6121 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     6181 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     6241 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     6301 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     6361 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     6421 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     6481 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     6541 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     6601 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     6661 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     6721 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     6781 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     6841 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     6901 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     6961 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     7021 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     7081 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     7141 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     7201 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgg
     7261 gtccttttca tcacgtgcta taaaaataat tataatttaa attttttaat ataaatatat
     7321 aaattaaaaa tagaaagtaa aaaaagaaat taaagaaaaa atagtttttg ttttccgaag
     7381 atgtaaaaga ctctaggggg atcgccaaca aatactacct tttatcttgc tcttcctgct
     7441 ctcaggtatt aatgccgaat tgtttcatct tgtctgtgta gaagaccaca cacgaaaatc
     7501 ctgtgatttt acattttact tatcgttaat cgaatgtata tctatttaat ctgcttttct
     7561 tgtctaataa atatatatgt aaagtacgct ttttgttgaa attttttaaa cctttgttta
     7621 tttttttttc ttcattccgt aactcttcta ccttctttat ttactttcta aaatccaaat
     7681 acaaaacata aaaataaata aacacagagt aaattcccaa attattccat cattaaaaga
     7741 tacgaggcgc gtgtaagtta caggcaagcg atccgtccta agaaaccatt attatcatga
     7801 cattaaccta taaaaatagg cgtatcacga ggccctttcg tc
//
