LOCUS       sequence-210582-        7821 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Truncated Hsh155 (aa 103-972).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7821)
  AUTHORS   Carrocci TJ, Paulson JC, Hoskins AA
  TITLE     Functional Analysis of Hsh155/SF3b1 Interactions with the U2 
            snRNA/Branch Site Duplex.
  JOURNAL   RNA. 2018 May 11. pii: rna.065664.118. doi: 10.1261/rna.065664.118.
  PUBMED    29752352
REFERENCE   2  (bases 1 to 7821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2018 
            May 11. pii: rna.065664.118. doi: 10.1261/rna.065664.118."
COMMENT     Sequence Label: Hsh155 103-972
FEATURES             Location/Qualifiers
     source          1..7821
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        187..467
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             468..1142
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(1241..1696)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1378..1399)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1590..1609
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1826..1848
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1840..1857
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1841..1857
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1867..1886
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1867..1885
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5047..5067)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5047..5065)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5086..5102)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5086..5102)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5099..5121)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5110..5126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5134..5140,5141..5158,5159..5164))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(5317..5334)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5488..6076)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5568..5587)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6247..7038,7039..7107))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6870..6889
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7108..7212)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    7249..7752
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     7794..7812
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaacg acattactat atatataata taggaagcat ttaatagaca gcatcgtaat
      241 atatgtgtac tttgcagtta tgacgccaga tggcagtagt ggaagatatt ctttattgaa
      301 aaatagcttg tcaccttacg tacaatcttg atccggagct tttctttttt tgccgattaa
      361 gaattaattc ggtcgaaaaa agaaaaggag agggccaaga gggagggcat tggtgactat
      421 tgagcacgtg agtatacgtg attaagcaca caaaggcagc ttggagtatg tctgttatta
      481 atttcacagg tagttctggt ccattggtga aagtttgcgg cttgcagagc acagaggccg
      541 cagaatgtgc tctagattcc gatgctgact tgctgggtat tatatgtgtg cccaatagaa
      601 agagaacaat tgacccggtt attgcaagga aaatttcaag tcttgtaaaa gcatataaaa
      661 atagttcagg cactccgaaa tacttggttg gcgtgtttcg taatcaacct aaggaggatg
      721 ttttggctct ggtcaatgat tacggcattg atatcgtcca actgcatgga gatgagtcgt
      781 ggcaagaata ccaagagttc ctcggtttgc cagttattaa aagactcgta tttccaaaag
      841 actgcaacat actactcagt gcagcttcac agaaacctca ttcgtttatt cccttgtttg
      901 attcagaagc aggtgggaca ggtgaacttt tggattggaa ctcgatttct gactgggttg
      961 gaaggcaaga gagccccgaa agcttacatt ttatgttagc tggtggactg acgccagaaa
     1021 atgttggtga tgcgcttaga ttaaatggcg ttattggtgt tgatgtaagc ggaggtgtgg
     1081 agacaaatgg tgtaaaagac tctaacaaaa tagcaaattt cgtcaaaaat gctaagaaat
     1141 aggttattac tgagtagtat ttatttaagt attgtttgtg cacttgccta tgcggtgtga
     1201 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt
     1261 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     1321 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     1381 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     1441 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     1501 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     1561 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     1621 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     1681 ccgctacagg gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     1741 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     1801 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag
     1861 cgcgcgtaat acgactcact atagggcgaa ttgggtaccg ggccccccct cgagcatgta
     1921 ctttccgcct aactaccatc acctgtcttt atttattccg ctgacagata tgcacaacca
     1981 attatcgtct cttgaaagct ttttagacgt gattgctgtt atttggcacg ttgttagtat
     2041 ttaagcttat tcgggtttgc attgatattt gcccgagcag aaatctcctt ctcgatgagt
     2101 tagaactgaa acatctcttc aattacttat acataataaa catagaaagc caggacctgt
     2161 catcatggta aaagggtacg agccacctga tgaatcttca acagcggtca aagaaaactc
     2221 tgatagtgct ttggttaatg tagaaggaat ccatgatttg atgtttttta aaccttctga
     2281 ccataaatat ttcgccgacg ttatatccaa aaaacccata gatgagttaa ataaggatga
     2341 aaagaaagaa agaaccctgt cgatgttact tctgaaaata aaaaatggca atacagcgag
     2401 taggaggact tctatgcgga ttttaaccga taaagctgtt acatttggtc cagaaatgat
     2461 atttaaccgc ttattgccca tattactaga cagaagtttg gaagaccagg aaagacatct
     2521 aatgatcaag accatagatc gcgtccttta ccagctagga gatttaacaa aaccttacgt
     2581 tcacaaaata cttgtagtag ctgccccatt gctaatagat gaagatccaa tggttcgttc
     2641 aacaggccag gagataatca ccaatctgtc tacagtcgct ggcctaaaaa ccattctaac
     2701 agtaatgaga cctgatattg agaatgagga tgagtatgtt agaaatgtaa cttcaagagc
     2761 ggcggctgtg gtggcaaaag cccttggtgt taatcaattg cttcctttca tcaatgcagc
     2821 atgccactca cggaagtctt ggaaagcaag gcatactggc ataaaaattg tgcagcaaat
     2881 aggtatactt ttgggaatag gtgtcttgaa tcatcttact gggctgatga gttgtatcaa
     2941 agactgccta atggatgatc acgttccggt gcgaattgtt acagcacata ctttatcaac
     3001 actagcagaa aattcttatc catatggtat cgaagttttc aacgttgtac tggagccatt
     3061 atggaagggt ataagaagtc atcgtggtaa agtgctatct tcttttttaa aagctgttgg
     3121 atctatgatc ccattgatgg acccagagta tgccggttat tataccacag aagcaatgag
     3181 aataattaga cgtgaattcg actctcccga tgatgaaatg aaaaagacca ttcttttagt
     3241 attacaaaaa tgtagcgctg tagaatccat aacgccaaaa tttctgagag aagaaatcgc
     3301 gccagaattc tttcaaaaat tttgggtcag acgagtggca ttggatagac cattaaacaa
     3361 agttgttaca tatactactg ttacattagc aaagaaatta ggttgttctt ataccattga
     3421 taaattatta acaccattga gagatgaagc tgagccgttc agaacaatgg ctgtccatgc
     3481 tgttactaga acagtaaact tattagggac agcagattta gatgagagat tagaaacgag
     3541 gcttattgat gcgctcttga tagcatttca agaacaaaca aatagtgatt ctataatctt
     3601 taaagggttt ggggcagtga cggtgtcact tgacatccgt atgaaaccgt ttttagcacc
     3661 cattgtgagt acgattttaa atcatttaaa gcataaaact cccttggttc gtcaacatgc
     3721 agcagatcta tgtgccatac taataccagt catcaagaac tgtcacgaat tcgaaatgtt
     3781 gaataaattg aacattatat tgtatgaatc cttaggcgaa gtatatccag aggtattagg
     3841 ttctataatc aacgctatgt actgtattac tagcgtaatg gatttggata aactacaacc
     3901 tcctattaat caaattttac caaccttaac tccaatttta agaaacaagc atagaaaagt
     3961 cgaagtaaac actatcaaat ttgttggact tattggtaaa ttagcaccta cctatgcacc
     4021 tcctaaagaa tggatgagaa tatgttttga actattagaa cttttgaaaa gtacaaataa
     4081 agaaataaga agatcagcaa atgccacgtt tggattcatt gctgaagcaa ttggacctca
     4141 cgatgttctt gttgcgttat tgaacaattt aaaggttcaa gaacgtcagt tacgtgtatg
     4201 cacggctgtt gctatcggta tcgttgctaa agtgtgtggt ccttacaacg tgctgccagt
     4261 aatcatgaat gaatacacaa caccagagac taatgttcaa aacggtgttc tcaaggccat
     4321 gtcattcatg tttgagtaca ttggtaacat gtctaaggat tacatatact tcataacacc
     4381 attattagaa gatgcactca ccgataggga tttagttcat cgtcagacag cgtcgaacgt
     4441 aataacccac ttagctttaa actgttcagg tacaggtcac gaagatgcct tcattcattt
     4501 aatgaatctt ctaataccca atatttttga aacttcacca catgctatta tgcgtatttt
     4561 agaaggtttg gaggcactga gtcaagctct tggccctgga ctattcatga actacatatg
     4621 ggcaggattg ttccatccag cgaagaatgt aaggaaagca ttttggaggg tgtataataa
     4681 catgtacgta atgtaccagg acgccatggt acctttttac cccgttacac cagacaacaa
     4741 tgaagaatat atagaagaac tggatttagt tctgtgaata tatatagagc atataaataa
     4801 ccacaacttg taagaatatt ttacttgaca cacctatatc atcacgtcac gatgccttca
     4861 tagattatga tgaaatataa acagaacctg ctattggaaa aaaaagggca ggaaaaaatt
     4921 ttgtgaaagt gagtaaaaga aagatgaaga gcgagacatg gataataaga aatagatgtc
     4981 actaatggac aattggaaga ctgatatgga aagttacgat gaaggaggag ctcccagctt
     5041 ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc
     5101 tgtgtgaaat tgttatccgc tcacaattcc acacaacata ggagccggaa gcataaagtg
     5161 taaagcctgg ggtgcctaat gagtgaggta actcacatta attgcgttgc gctcactgcc
     5221 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     5281 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     5341 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     5401 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     5461 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     5521 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     5581 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     5641 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     5701 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     5761 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     5821 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     5881 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     5941 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     6001 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     6061 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     6121 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     6181 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     6241 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     6301 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     6361 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     6421 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     6481 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     6541 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     6601 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     6661 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     6721 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     6781 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     6841 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     6901 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     6961 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     7021 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     7081 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     7141 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     7201 aggggttccg cgcacatttc cccgaaaagt gccacctggg tccttttcat cacgtgctat
     7261 aaaaataatt ataatttaaa ttttttaata taaatatata aattaaaaat agaaagtaaa
     7321 aaaagaaatt aaagaaaaaa tagtttttgt tttccgaaga tgtaaaagac tctaggggga
     7381 tcgccaacaa atactacctt ttatcttgct cttcctgctc tcaggtatta atgccgaatt
     7441 gtttcatctt gtctgtgtag aagaccacac acgaaaatcc tgtgatttta cattttactt
     7501 atcgttaatc gaatgtatat ctatttaatc tgcttttctt gtctaataaa tatatatgta
     7561 aagtacgctt tttgttgaaa ttttttaaac ctttgtttat ttttttttct tcattccgta
     7621 actcttctac cttctttatt tactttctaa aatccaaata caaaacataa aaataaataa
     7681 acacagagta aattcccaaa ttattccatc attaaaagat acgaggcgcg tgtaagttac
     7741 aggcaagcga tccgtcctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     7801 gtatcacgag gccctttcgt c
//
