LOCUS       sequence-210584-        7659 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Truncated Hsh155 (aa 157-972).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7659)
  AUTHORS   Carrocci TJ, Paulson JC, Hoskins AA
  TITLE     Functional Analysis of Hsh155/SF3b1 Interactions with the U2 
            snRNA/Branch Site Duplex.
  JOURNAL   RNA. 2018 May 11. pii: rna.065664.118. doi: 10.1261/rna.065664.118.
  PUBMED    29752352
REFERENCE   2  (bases 1 to 7659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2018 
            May 11. pii: rna.065664.118. doi: 10.1261/rna.065664.118."
COMMENT     Sequence Label: Hsh155 157-972
FEATURES             Location/Qualifiers
     source          1..7659
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        187..467
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             468..1142
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(1241..1696)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1378..1399)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1590..1609
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1826..1848
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1840..1857
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1841..1857
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1867..1886
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1867..1885
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4885..4905)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4885..4903)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4924..4940)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4924..4940)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4937..4959)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4948..4964
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4972..4978,4979..4996,4997..5002))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(5155..5172)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5326..5914)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5406..5425)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6085..6876,6877..6945))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6708..6727
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6946..7050)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    7087..7590
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     7632..7650
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaacg acattactat atatataata taggaagcat ttaatagaca gcatcgtaat
      241 atatgtgtac tttgcagtta tgacgccaga tggcagtagt ggaagatatt ctttattgaa
      301 aaatagcttg tcaccttacg tacaatcttg atccggagct tttctttttt tgccgattaa
      361 gaattaattc ggtcgaaaaa agaaaaggag agggccaaga gggagggcat tggtgactat
      421 tgagcacgtg agtatacgtg attaagcaca caaaggcagc ttggagtatg tctgttatta
      481 atttcacagg tagttctggt ccattggtga aagtttgcgg cttgcagagc acagaggccg
      541 cagaatgtgc tctagattcc gatgctgact tgctgggtat tatatgtgtg cccaatagaa
      601 agagaacaat tgacccggtt attgcaagga aaatttcaag tcttgtaaaa gcatataaaa
      661 atagttcagg cactccgaaa tacttggttg gcgtgtttcg taatcaacct aaggaggatg
      721 ttttggctct ggtcaatgat tacggcattg atatcgtcca actgcatgga gatgagtcgt
      781 ggcaagaata ccaagagttc ctcggtttgc cagttattaa aagactcgta tttccaaaag
      841 actgcaacat actactcagt gcagcttcac agaaacctca ttcgtttatt cccttgtttg
      901 attcagaagc aggtgggaca ggtgaacttt tggattggaa ctcgatttct gactgggttg
      961 gaaggcaaga gagccccgaa agcttacatt ttatgttagc tggtggactg acgccagaaa
     1021 atgttggtga tgcgcttaga ttaaatggcg ttattggtgt tgatgtaagc ggaggtgtgg
     1081 agacaaatgg tgtaaaagac tctaacaaaa tagcaaattt cgtcaaaaat gctaagaaat
     1141 aggttattac tgagtagtat ttatttaagt attgtttgtg cacttgccta tgcggtgtga
     1201 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt
     1261 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     1321 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     1381 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     1441 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     1501 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     1561 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     1621 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     1681 ccgctacagg gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     1741 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     1801 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag
     1861 cgcgcgtaat acgactcact atagggcgaa ttgggtaccg ggccccccct cgagcatgta
     1921 ctttccgcct aactaccatc acctgtcttt atttattccg ctgacagata tgcacaacca
     1981 attatcgtct cttgaaagct ttttagacgt gattgctgtt atttggcacg ttgttagtat
     2041 ttaagcttat tcgggtttgc attgatattt gcccgagcag aaatctcctt ctcgatgagt
     2101 tagaactgaa acatctcttc aattacttat acataataaa catagaaagc caggacctgt
     2161 catcatgaat aaggatgaaa agaaagaaag aaccctgtcg atgttacttc tgaaaataaa
     2221 aaatggcaat acagcgagta ggaggacttc tatgcggatt ttaaccgata aagctgttac
     2281 atttggtcca gaaatgatat ttaaccgctt attgcccata ttactagaca gaagtttgga
     2341 agaccaggaa agacatctaa tgatcaagac catagatcgc gtcctttacc agctaggaga
     2401 tttaacaaaa ccttacgttc acaaaatact tgtagtagct gccccattgc taatagatga
     2461 agatccaatg gttcgttcaa caggccagga gataatcacc aatctgtcta cagtcgctgg
     2521 cctaaaaacc attctaacag taatgagacc tgatattgag aatgaggatg agtatgttag
     2581 aaatgtaact tcaagagcgg cggctgtggt ggcaaaagcc cttggtgtta atcaattgct
     2641 tcctttcatc aatgcagcat gccactcacg gaagtcttgg aaagcaaggc atactggcat
     2701 aaaaattgtg cagcaaatag gtatactttt gggaataggt gtcttgaatc atcttactgg
     2761 gctgatgagt tgtatcaaag actgcctaat ggatgatcac gttccggtgc gaattgttac
     2821 agcacatact ttatcaacac tagcagaaaa ttcttatcca tatggtatcg aagttttcaa
     2881 cgttgtactg gagccattat ggaagggtat aagaagtcat cgtggtaaag tgctatcttc
     2941 ttttttaaaa gctgttggat ctatgatccc attgatggac ccagagtatg ccggttatta
     3001 taccacagaa gcaatgagaa taattagacg tgaattcgac tctcccgatg atgaaatgaa
     3061 aaagaccatt cttttagtat tacaaaaatg tagcgctgta gaatccataa cgccaaaatt
     3121 tctgagagaa gaaatcgcgc cagaattctt tcaaaaattt tgggtcagac gagtggcatt
     3181 ggatagacca ttaaacaaag ttgttacata tactactgtt acattagcaa agaaattagg
     3241 ttgttcttat accattgata aattattaac accattgaga gatgaagctg agccgttcag
     3301 aacaatggct gtccatgctg ttactagaac agtaaactta ttagggacag cagatttaga
     3361 tgagagatta gaaacgaggc ttattgatgc gctcttgata gcatttcaag aacaaacaaa
     3421 tagtgattct ataatcttta aagggtttgg ggcagtgacg gtgtcacttg acatccgtat
     3481 gaaaccgttt ttagcaccca ttgtgagtac gattttaaat catttaaagc ataaaactcc
     3541 cttggttcgt caacatgcag cagatctatg tgccatacta ataccagtca tcaagaactg
     3601 tcacgaattc gaaatgttga ataaattgaa cattatattg tatgaatcct taggcgaagt
     3661 atatccagag gtattaggtt ctataatcaa cgctatgtac tgtattacta gcgtaatgga
     3721 tttggataaa ctacaacctc ctattaatca aattttacca accttaactc caattttaag
     3781 aaacaagcat agaaaagtcg aagtaaacac tatcaaattt gttggactta ttggtaaatt
     3841 agcacctacc tatgcacctc ctaaagaatg gatgagaata tgttttgaac tattagaact
     3901 tttgaaaagt acaaataaag aaataagaag atcagcaaat gccacgtttg gattcattgc
     3961 tgaagcaatt ggacctcacg atgttcttgt tgcgttattg aacaatttaa aggttcaaga
     4021 acgtcagtta cgtgtatgca cggctgttgc tatcggtatc gttgctaaag tgtgtggtcc
     4081 ttacaacgtg ctgccagtaa tcatgaatga atacacaaca ccagagacta atgttcaaaa
     4141 cggtgttctc aaggccatgt cattcatgtt tgagtacatt ggtaacatgt ctaaggatta
     4201 catatacttc ataacaccat tattagaaga tgcactcacc gatagggatt tagttcatcg
     4261 tcagacagcg tcgaacgtaa taacccactt agctttaaac tgttcaggta caggtcacga
     4321 agatgccttc attcatttaa tgaatcttct aatacccaat atttttgaaa cttcaccaca
     4381 tgctattatg cgtattttag aaggtttgga ggcactgagt caagctcttg gccctggact
     4441 attcatgaac tacatatggg caggattgtt ccatccagcg aagaatgtaa ggaaagcatt
     4501 ttggagggtg tataataaca tgtacgtaat gtaccaggac gccatggtac ctttttaccc
     4561 cgttacacca gacaacaatg aagaatatat agaagaactg gatttagttc tgtgaatata
     4621 tatagagcat ataaataacc acaacttgta agaatatttt acttgacaca cctatatcat
     4681 cacgtcacga tgccttcata gattatgatg aaatataaac agaacctgct attggaaaaa
     4741 aaagggcagg aaaaaatttt gtgaaagtga gtaaaagaaa gatgaagagc gagacatgga
     4801 taataagaaa tagatgtcac taatggacaa ttggaagact gatatggaaa gttacgatga
     4861 aggaggagct cccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc
     4921 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatagg
     4981 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgaggtaac tcacattaat
     5041 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     5101 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     5161 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     5221 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     5281 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     5341 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     5401 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     5461 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     5521 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     5581 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     5641 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     5701 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     5761 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     5821 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     5881 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     5941 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     6001 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     6061 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     6121 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     6181 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     6241 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     6301 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     6361 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     6421 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     6481 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     6541 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     6601 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     6661 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     6721 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     6781 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     6841 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     6901 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     6961 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     7021 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgggtc
     7081 cttttcatca cgtgctataa aaataattat aatttaaatt ttttaatata aatatataaa
     7141 ttaaaaatag aaagtaaaaa aagaaattaa agaaaaaata gtttttgttt tccgaagatg
     7201 taaaagactc tagggggatc gccaacaaat actacctttt atcttgctct tcctgctctc
     7261 aggtattaat gccgaattgt ttcatcttgt ctgtgtagaa gaccacacac gaaaatcctg
     7321 tgattttaca ttttacttat cgttaatcga atgtatatct atttaatctg cttttcttgt
     7381 ctaataaata tatatgtaaa gtacgctttt tgttgaaatt ttttaaacct ttgtttattt
     7441 ttttttcttc attccgtaac tcttctacct tctttattta ctttctaaaa tccaaataca
     7501 aaacataaaa ataaataaac acagagtaaa ttcccaaatt attccatcat taaaagatac
     7561 gaggcgcgtg taagttacag gcaagcgatc cgtcctaaga aaccattatt atcatgacat
     7621 taacctataa aaataggcgt atcacgaggc cctttcgtc
//
